載體化合物 的英文怎麼說
中文拼音 [zǎitǐhuàgěwù]
載體化合物
英文
carrier compound-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ ( 95 )株接種spf雞胚繁殖病毒,經處理后提取病毒的基因組rna ,參考國內外發表的ndv融合蛋白基因序列,設計一對特異性引物,經反轉錄聚合酶鏈式反應( rt - pcr )擴增出約1700bp大小的特異性片段,將此片段回收純化后,利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中,再轉化大腸桿菌jm109感受態細胞,轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。Chiral quaternary ammonium salts were synthesized from cinchonine in cinchona alkaloids using chloromethylated polystyrene - polyethylene glycol, diethylene glycol, triethylene glycol and glycol as polymer - supported phase transfer catalysts, finsl products were characterized by ftir, elemental analysis, scanning electron microscopy, x - ray photoelectron spectroscopy ( xps )
摘要以金雞納生物堿中的辛可寧為原料,氯甲基化聚苯乙烯聚乙二醇、一縮二乙二醇、二縮三乙二醇、乙二醇等為載體合成了幾種聚合物負載的手性季銨鹽,並對它們的結構用紅外光譜、元素分析、掃描電鏡和x射線光電子能譜等測試手段進行了表徵。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。Macromolecules cannot approach the ligand owing to steric hindrance of the matrix.
由於載體的立體位阻的關系,大分子化合物不能接觸到配基。Construction of an expressing vector for antisense rna - ribozyme chimeric dna sequence against tomato acc synthase gene and transformation of tomato
核酶嵌合基因植物表達載體的構建及對番茄的轉化As a popular vegetable, tomato is rich in vatamin a, b, p and other nutrients ; among them, lyxopene can prevent prostate gland tumor. so introducing stilbene synthase gene into tomato to get new health - protection tomato will have important social and economic effect. our research is made up of four parts ; the cloning and sequencing of stilbene synthase ; tomato genetic transforming mediated by agrobacterium - lba4404 ; quantification of resveratrol in transgenic tomato by hplc analysis
本研究包括四個方面的內容:芪合酶基因( stilbenesynthasegene )的克隆與全序列分析;芪合酶基因( stilbenesynthasegene )植物表達載體的構建;農桿菌lba4404介導的番茄遺傳轉化: hplc技術分析轉基因植株中表達白藜蘆醇的含量。The perovskite - type metal oxides supports and the corresponding supported pd catalyst were prepared by microwave, and characterized by xrd, sem and used in the direct synthesis of diphenyl carbonate ( dpc )
摘要利用微波法制備了鈣鈦礦型復合氧化物載體及把負載鈣鈦礦型催化劑,用xrd和sem對載體及催化劑進行了表徵,並將微波法制備的催化劑用於一步氧化羰基化合成碳酸二苯酯( dpc )實驗。2. an up - dated method was employed to purify tumv in this research. using the protease k method, we acquired the viral genome - rna. a pair of specific primers was designed and synthesized based on the nucleotide sequences of tumv coat protein genes reported before and rt - pcr was used to clone the cp genes of the six tumv isolates
應用改進的蕪菁花葉病毒的提取方法從病葉中提取病毒粒體,應用蛋白酶k法從病毒粒體中提取病毒rna基因組,根據已報道的tumv的cp基因序列,設計併合成了一對特異引物,利用rt - pcr法克隆了6個分離物的外殼蛋白基因,與克隆載體puc19連接后通過熱激法轉化大腸桿菌dh5 。Then introduce the present situation service of cosco - logistics ltd : appliance logistics, car logistics, electric power logistic, petrifaction logistics, exhibit logistics and retail logistics. on the base of swot analysis of cosco - logistics ltd, chapter four gives suggestion to logistics service innovation of cosco - logistics ltd : developing electronic commerce, integrated marketing, customization logistics and 4pl ? increment service. then give some supports strategy for logistics service innovation of cosco - logistics ltd
第四章通過對中遠物流的swot分析,結合物流服務發展的趨勢,提出了中遠物流企業開展服務創新應該以電子商務為載體、以一體化營銷為基礎、以客戶訂制和第四方物流為發展方向、以增值服務為中遠物流的核心競爭力,在此基礎上,提出了開展物流創新所需要的物流設備的現代化、物流資源整合、物流信息化、物流人才的培養等方面的支持政策。Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays
為了便於轉基因棉花後代的篩選,在pap基因的3 』端融入了綠色熒光蛋白gfp )基因,然後將融合基因克隆在植物表達載體pbi121上,再進行遺傳轉化,得轉基因棉花種子5000餘粒,將種子播種長到于葉展開時,先在黑暗中用紫外燈照射,查找表現綠色熒光的幼苗,然後再用地高辛( dig )標記的pap基因特異性探針對這些棉花進行點雜交,最後發現有8株棉花表現陽性反應,說明pap基因的確己經轉到了棉花的基因組中,其棉花黃萎病的抗性鑒定正在進行之中。The factors affecting catalytic properties of supported heteropoly compounds and the interaction between the heteropoly compounds and the supports are also discussed
對雜多化合物與載體之間的相互作用以及雜多化合物催化劑的反應機理進行了討論。The enhanced photoconductive effect from small amount of tnf facilitates the preparations of new organic photoconductive devices under the drive of low fields. in the fourth chapter, inclpc nanoparticles embedded in poly ( n - vinylcarbzaole ) ( pvk ) were prepared successfully by dissolving inclpc in aprotic organic solvent / lewis acid with great concentration for the formation of electron donor - acceptor complexes, i. e., the method of complexation - mediated solubilization. the fabricated inclpc nanoparticles were characterized by means of uv / vis absorption, x - ray diffraction pattern, and tem
論文的最後一章中,我們合成了具有較好的電子傳輸性能的化合物』一二苯基四竣酸花酚亞胺( ddp ) ;研究了其溶解性、熱穩定性、晶體結構、紅外光譜、紫外吸收光譜和蒸鍍薄膜的屬性,並用量子化學計算方法模擬其單分子的空間構型;載流子遷移率測試的結果約為ix10 「 、 m 』 v 」 』 ? s 「 』 。( 9 ) by studying matching between catalytic converter and exhaust gas system, the conclusions having been gotten as follow : " purifying step by step and driving step by step " and " mental substrate and chinaware substrate being used in same engine " being the laying principles in exhaust gas system, which can solve the contradiction between high converting efficiency of exhaust emission and small loss of power, fuel economics, the rapid light - off and laying difficulty of catalyst
( 9 )進行了催化轉化器與排氣系統的匹配研究,對于催化劑的布置,提出了「分級凈化,逐級推動」的原則;對于載體形式的選擇,提出了「金屬載體和陶瓷載體復合」使用的原則。這樣可以良好的解訣催化轉化器與發動機匹配時,排氣污染物高凈化率與動力性經濟性損失小之間的矛盾、快速起燃和結構布置困難的矛盾。This thesis studies the interaction of dna with some metal schiff - base complexes with fluorescence spectra, the model of the interaction were investigated in details, and two specific anticancer drugs were obtained. the detailed materials are shown as follows : this first part : renewable amperometric imn ~ iunosensors have been developed with paraffin as carrier
在本論文中,我們採用熒光光譜法研究了一些席夫堿類金屬配合物傳感載體與dna的相互作用,對其相互作用模式作了詳細的探討,篩選出二種與dna有強烈相互作用的化合物。The complexes of schiffbase are not only the carriers of cation on potentiometric sensors but also anticancer drugs. it can induce or cleave dna in the present of cooxidant, and its anticancer effect increase when metal ions complex it
席夫堿化合物可以作為許多陰離子電位型傳感器的載體,同時也是一類具有抗癌活性的化合物,在氧化物存在的條件下,能損傷或者斷裂dna ,與金屬離子結合以後,其抗癌效果增強。Polymer networks have been produced in a variety of liquid crystal phases in cells without surface treatment. the liquid crystal / monomer mixtures are prepared using a ferroelectric liquid crystal and a diacrylate monomer. the polymerisation of the monomer is carried out by uv curing the mixture when it was kept in a specific phase which can be obtained by controlling the temperature of the ferroelectric liquid crystal. both the monomer and the polymer network will depress phase transition temperatures. the effects of the polymer network on the packing arrangement of the ferroelectric liquid crystal molecules are examined by means of optical microscopy. the molecular packing arrangement of the ferroelectric liquid crystal in a specific mesogenic phase is frozen on the formation of a polymer network in the ferroelectric liquid crystal in that phase. the existence of the diacrylate based polymer network can improve the electrooptical switching of the ferroelectric liquid crystal
在灌裝于未經表面處理的樣品盒內處于不同狀態的液晶中制備聚合體網路.液晶/單體混合物由鐵電液晶和雙丙烯酸單體製成.單體的聚合通過維持該混合物處在一定的液晶態的條件下經紫外線固化來完成.不同的液晶態可通過控制鐵電液晶的溫度來獲得.雙丙烯酸單體或聚合體網路的存在會導致鐵電液晶載體的相變溫度降低.通過光學顯微鏡可觀察聚合體網路對鐵電液晶分子組合排列的影響.結果發現:在某一液晶態中鐵電液晶分子的組合排列可被在該液晶態中聚合而成的聚合體網路所穩定,雙丙烯酸基聚合體網路的存在可改良鐵電液晶的電光開關特性Kaolinite - organic intercalation compound possesses not only the unique adsorptivity, dispersivity. porosity, rheological behaviour and surface acidity of the clay mineral but also the multivariate functional groups and the reactivity of the organic compound. as a new type of mineral materials, it has extensive potential application in catalysts, functional supporter, adsorbents and advance ceramics
高嶺石有機插層復合物既具有粘土礦物特有的吸附性、分散性、流變性、多孔性和表面酸性,又具有機化合物的多變功能團和反應活性,作為新型礦物材料,在催化劑、功能載體、吸附劑、先進陶瓷材料等方面具有廣闊的應用前景。The ci ~ - c : h film was prepared by the means of plasma assistance chemical vapor deposition with hydrocarbon n - butylamine ( ch3ch2ch2ch2nh2 ) as carbon source. the material of carbon source was carried into chemical vapor deposition chamber under pure hydrogen
採用等離子體輔助化學氣相沉積方法,以碳氫化合物正丁胺( ch _ 3ch _ 2ch _ 2ch _ 2nh _ 2 )作為碳源物質,用高純氫氣作為載氣,將碳源物質攜帶進入反應室。Abstract : a review with 6 references is given on some formatities of trace element supplement for people including natural foods, inorganic chemicals, organic chemicals, complex trace element drugs and trace element ferments
文摘:論述了人體補充微量元素的幾種主要方式,其中包括天然食物、無機化合物、有機化合物、復合微量元素藥物和微量元素載體酵母等。Searching for novel porphyrin derivatives, including corrole, with high selectivity as the molecular recognition ionophores for chemical sensors, therefore, remains task of theoretical and practical significance. the contents of the thesis are presented as follows : 1. the syntheses of series of porphyrin derivatives were presented in the second charpter
以卟啉及其金屬配合物為分子識別載體的化學傳感器件研究是一個十分活躍的領域,尋找具高選擇性的包括可咯在內的新型卟啉類化合物用於化學傳感研究仍然是一項非常有意義的工作。分享友人