轉化細胞 的英文怎麼說
中文拼音 [zhuǎnhuàxìbāo]
轉化細胞
英文
transformant- 轉 : 轉構詞成分。
- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 轉化 : 1. (轉變) change; transform 2. [化學] inversion; conversion
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
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Study of " ph memory " and activity water ( aw ) in processing of immobilization indicates that former factor has much effect on transformation and the latter just has little effect. permeabilization treating of baker ' s cell with alcohol and ultrasonic leads to lower transformation rate, which illustrates that permeabilization procedure has negative role on biotransformation with whole cell as biocatalyst in organic solvent. in simple dynamics research amount of biocatalysu concentration of substrate and reaction course are considered and all of them have remarkable effect on transformation
Ph記憶、水活度及通透性處理的實驗結果表明, ph記憶對轉化有明顯的影響,水活度對模型反應的影響不大;作為完整細胞生物催化,細胞的通透性可能是傳質阻力的重要組成部分,利用不同濃度的乙醇和超聲波對細胞進行處理,結果表明,在有機相完整細胞生物催化中,通透性處理對轉化產生的是負面影響。The potential for a wide range of biotransformations using either immobilized cells or extracted plant enzymes undoubtedly exists, the major restriction to development being our lack of fundamental knowledge of the general biochemistry and enzymology of natural product synthesis
盡管我們在自然產品合成方面的普通生物化學和酶學知識還很匱乏,但使用固定化細胞或植物提取酶的生物轉化得到大范圍應用的可能性無疑是存在的。Transformed cells are loosely attached, rounded and randomly oriented.
轉化細胞則不大貼壁、圓縮並呈雜亂分佈。These cells exhibited the glycopetide pattern characteristic of transformed cells when grown at the permissive temperature.
這些細胞在許可的溫度下生長時,具有轉化細胞所特有的糖肽圖譜。People ' s blood vessel and lymphatic gland are served as by certain degree squeezing and are loosened, meeting boost blood and the lymph cycling, make the body arrange poison, and energy activation cell effectively, make the obstinate fat change into fatty acid, reach the towline body effect reducing weight thereby
當人們的血管和淋巴腺受到一定程度的擠壓和放鬆,會促進血液和淋巴液循環,使身體有效地排毒,並能活化細胞,令頑固的脂肪轉化為脂肪酸,從而達到減肥纖體作用!Metformin, an antihyperglycemic agent used in type 2 diabetes, was also inestigated because it inhibits ptp opening in transformed cell lines
二甲雙胍是用於治療2型糖尿病的降糖藥,能夠抑制轉化細胞系ptp的開放,所以我們對其進行調查After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化,細胞裂解物的sds - page電泳, westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示:重組質粒轉染的細胞質中有棕褐色顆粒,而空載體轉染細胞及正常細胞無此現象;細胞裂解物sds - page電泳結果顯示:只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶,這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致; western - blot分析結果顯示:約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清,成蟲蟲體可溶性抗原免疫兔血清, ts87基因原核表達蛋白免疫兔血清( ts87血清)以及一株具保護性的旋毛蟲單抗特異識別。Apoptin ( vp, ) can selectively induce apoptosis in human transformed and malignant cells but not in normal diploid cells. apoptin induces a p53 - independent, bcl - 2 - insensitive type of apoptosis in various human tumor cells
其中, vp _ 3蛋白質是雞貧血病毒的致病因子,能選擇性的誘導腫瘤細胞和轉化細胞發生凋亡,但對人和鼠的正常二倍體細胞無影響,稱為凋亡素( apoptin ) 。Thioredoxins, an ubiquitous small proteins with a redox active disulfide bridge in its conserved motif - cp ( g ) pc -, are universally distributed in eucaryote and procaryote and have a molecular mass of approximately 12kda. by its disulfide / dithiol interchange reaction, this protein can transmit the regulatory signals to seleted targets ( enzymes, transcription factors etc ) and plays an important role in many plant physiological processes that includes photosynthesis, dna synthesis, transcription, protein disulfide reduction, protein repair, filamentous phage assembly, cell apoptosis and seeds germinating and so on
該蛋白質中含有保守的- cp ( g ) pc -氨基酸活性基序,該基序中的兩個半胱氨酸殘基可通過巰基二硫鍵的轉換實現其氧化還原狀態的變化和電子氫的傳遞,對細胞中與氧化還原相關的多種生理過程的調節起重要作用。通過同許多酶類、蛋白類、細胞內活性因子相藕連, trx能對光合作用、 dna復制、基因轉錄、細胞凋亡和生長、噬菌體組裝、蛋白質的還原和修復信號傳導等生理過程產生影響和調節。Epigenetic modification of the genome ensures proper gene activation during development and involves : genome methylation changes ; the assembly of histon and histone variants into nucleosomes and remodeling of other chromatin - associated proteins such as linker histones, polycomb group, nuclear scaffold protein, and transcription factors
這些染色質的再程序化包括dna的甲基化和核小體組蛋白的乙酰化等。組蛋白乙酰化在染色質結構調節、 dna復制、基因轉錄、細胞分化及癌細胞發育都有研究,但在克隆動物中卻是一個有待深入探索的領域。In our laboratory, a unique mutation detection system using a shuttle vector plasmid has been established to demonstrate that a low concentration of mnng ( 0. 2 m ) can induce nontargeted mutation in mammalian cells : the mammalian cells were exposed to 0. 2m mnng for 2. 5h, then a shuttle plasmid pz189 carrying supf trna gene was transfected into cells after 24h culture. we found a 5 - fold higher mutation frequency of the plasmid replicated in pretreated cells than the spontaneous mutation frequency of the plasmid replicated in control cells. this kind of mutation did not occur immediately after mnng exposure
我們實驗室曾用一特殊的突變檢測系統,直接證明dna損傷劑可在哺乳動物細胞誘發非定標性突變:首先用低濃度( 0 . 2 m )的短壽烷化劑mnng (半壽期為1 . 1hr )處理細胞2 . 5h后,繼續培養24h ,將重組有用作突變檢測的靶基因supftrna基因的穿梭質粒pz189轉入細胞復制,發現在未受致癌物直接攻擊的穿梭質粒中有較自發突變率高5倍以上的靶基因突變。Agrobacterium rhizogenes ( rhizobiacease ) is a kind of gram - negative bacterium, which contains a big ri plasmid ( 200kb ) - root inducing plasmid. there are three types of ri plasmid, mannopine type, cucumopine type and agropine type, according to the type of opine synthesized in transgenic cell
根據轉化細胞合成的冠癭堿( opine )的類型不同, ri質粒分為3種類型:甘露堿型( mannopinetype ) 、黃瓜堿型( cucumopinetype )和農桿堿型( agropinetype ) 。The most striking illustration of this is the isolation of untransformed revertant cells from populations of transformed cells.
這方面最顯著的例證乃是從轉化細胞群體中分離出非轉化的返轉細胞。There are two egfr signaling pathways, one is the classical ras / raf / mek / mapk pathway and the other is pi - 3 - k / pkb pathway. in this part, the phosphorylation and / or activity of some key molecules in these two signaling pathways in differently transfected h7721 cells were investigated. by means of specific antibodies combined with weste
Egf刺激后不同轉染細胞中的pkb活性、 p42 / 44mapk和mek磷酸化都明顯增加,其中gntv一s / h7721細胞pkb比活性、 p42 / 44mapk和mek磷酸化程度較mock細胞增加明顯,而gntv一as / h7721細胞中上述指標的增加程度則不及mock細胞。Restin was a novel gene isolated by our lab from differentiation tumor cell induced by all - trans retinoic acid in 1999, and its molecular functions were not well understood. it mainly expresses in terminally differentiated cells. by transfection of restin into tumor cell, the cells were arrested in g1 phase and the cell proliferation was inhibited
Restin是1999年本室在研究全反式維甲酸誘導細胞分化時克隆得到的一個人類未知功能的新基因,該基因主要表達于終末分化細胞,穩定轉染該基因的細胞出現g1期阻滯,抑制細胞的增殖,提示restin與細胞周期的調控有關。Except for 1. 0 kb hybridization band, other different hybridization bands were also detected which demonstrated that lea3 gene integrated into strawberry genome in different loci or copies
Okb雜交帶外,還有其他片段人小不等的雜交帶。不同的株系形成不同的雜交譜,表明這些轉化植株來自不同的轉化細胞。( 6 ) pdgf may initiate neuronal differentiation in nsc. nsc induced by pdgf, transcription and translation level of different thrs genes were up - regulated compared with the nsc induced by serum. the results made a great foundation for the further research of the important role of thrs during the cns development, and have much clinical significance on therapy of cretinism ^ subcretinism a
Thr各亞型在不同分化細胞中表達不一致: ( 0l ;能誘導nsc向少突膠質細胞分化,並通過thr各亞型的時空表達來發揮其重要生理作用門印叩樸能引發vc向神經幾分化,與10血清誘導分化相比能在轉錄與翻譯水平上提高nsc分化后thr各亞型的表達本研究結果將為深入研究t在腦發育中的作用提供寶貴的實驗依據,並對克汀病、亞克汀病以及ms脫髓鞘病等的臨床治療提供新途徑,具有重要指導意義。Transfection of 3t3 - l1 preadipocytes with a c - crkii antisense rna expression vector markedly reduced c - crkii levels and prevented differentiation into adipocytes
將c - crkii的反義rna轉化細胞能夠顯著的降低細胞內c - crkii的表達水平並抑制脂肪細胞的分化。It was proved that the protein was got by sds - page. the successful cloning and expressing of vp3 gene become the basis for the study and exploiture of apoptin
目前,對凋亡素的研究主要集中在其促進腫瘤和轉化細胞選擇性凋亡的功能方面,而對其作用機理和空間結構方面的報道較少。The results showed that the salt tolerance of transgenic strawberry was increased, the development of damage symptoms ( wilting ) caused by salt stress was delayed, and the recovery improved when salt stress conditions were removed
結果表明, lea3基岡導入草譴細胞后,與對照相比轉化細胞再生植株抗鹽性明顯增強,並且在受到鹽脅迫時,轉化株萎蔫癥狀出現晚,受害程度輕,一口分享友人