轉化酶原 的英文怎麼說

中文拼音 [zhuǎnhuàyuán]
轉化酶原 英文
proconvertase
  • 轉 : 轉構詞成分。
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 原 : Ⅰ形容詞1 （最初的; 原來的） primary; original; former 2 （沒有加工的） unprocessed; raw Ⅱ動詞（原...
  • 轉化 : 1. （轉變） change; transform 2. [化學] inversion; conversion
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. The principle and method for enzymatic synthesis of gallic acid, isolation and selection of the aspergillus niger strains, characteristics of this biotechnology, products quality of gallic acid and the uses in domestic food and pharmaceutical industries are briefly introduced

    摘要概述了黑麴黴單寧酶酶法轉化五倍子單寧酸生產沒食子酸的原理和方法、酶源菌種的分離和選育、工藝技術的特點、產品質量規格及在國內食品、醫藥行業相關部門的應用等。
  3. The quality of an enzyme immunoassays depends very much on the purity of the antigen or hapten used for conjugation, the specificity of the antibody and the choice of a suitable enzyme label

    酶免疫分析技術的質量依賴于抗原的純度、抗體的特異性、合適標記酶的選用，其靈敏度取決于標記酶的高度純化和高轉化率。
  4. Inhibitors of cyp51, such as azalanstat ( rs - 2i607 ) and rs - 2i745, could inhibit the synthesis of ff - mas to decrease the accumulation of ff - mas. inhibitor of a14 - reductase, such as ay9944 - a - 7, could inhibit the metabolism of ff - mas to increase the accumulation of ff - mas ; and some other reagents, such as nystatin, could combine with the downstream intermediate in the cholesterol biosynthesis pathway to accumulate mas. in this study, we investigated the role of mas by using these reagents to change the level of endogenous ff - mas

    抑制cyp51酶的抑制劑，如azalanstat （ rs - 21607 ）和rs - 21745等，均能抑制mas的合成，降低mas的積累；而抑制14 -還原酶的抑制劑，如ay9944 - a - 7等，能抑制ff - mas向t - mas的轉化而造成ff - mas的積累；還有一些物質，如制黴菌素，能阻止mas向下游的代謝而造成其積累，本文主要通過應用這些物質降低、或增加組織細胞中內源性mas的積累，來研究mas的作用。
  5. In the stomach, this acid functions to kill bacteria in foods, to soften foods and to convert the inactive enzyme pepsinate into its active from pepsin, to begin the digestion of protein

    在胃裡，這種酸的作用是殺司食品的細菌，使食物軟化，把鈍化的胃蛋白酶原轉化為有活性的能消化蛋白質的胃蛋白酶。
  6. The 11 - hsd1 expression in the fetal brain also gradually increases with gestational age at late gestation and continues to increase in the hippocampus after birth until the 15th postnatal day, which shows a pattern very similar to th secretion. furthermore, previous data show injection of thyroxine ( t4 ) to normal newborn rats up - regulates gr expression in the hippocampus. these data suggest that the role of th in neural development may be partially ( if not all ) accomplished through regulating gc ' s activity

    在海馬結構， 11 - hsd1與gr共存於同一海馬神經元內，主要呈現還原酶的特性，能使無活性的11 -脫氫皮質酮（ a ）可的松（ e ）轉化為有活性的皮質酮（ b ）皮質醇（ f ） ，從而提高組織局部的糖皮質激素濃度，這樣， 11 - hsd1的存在就為親和力較低的gr發揮作用提供了前提條件。
  7. Thioredoxins, an ubiquitous small proteins with a redox active disulfide bridge in its conserved motif - cp ( g ) pc -, are universally distributed in eucaryote and procaryote and have a molecular mass of approximately 12kda. by its disulfide / dithiol interchange reaction, this protein can transmit the regulatory signals to seleted targets ( enzymes, transcription factors etc ) and plays an important role in many plant physiological processes that includes photosynthesis, dna synthesis, transcription, protein disulfide reduction, protein repair, filamentous phage assembly, cell apoptosis and seeds germinating and so on

    該蛋白質中含有保守的- cp （ g ） pc -氨基酸活性基序，該基序中的兩個半胱氨酸殘基可通過巰基二硫鍵的轉換實現其氧化還原狀態的變化和電子氫的傳遞，對細胞中與氧化還原相關的多種生理過程的調節起重要作用。通過同許多酶類、蛋白類、細胞內活性因子相藕連， trx能對光合作用、 dna復制、基因轉錄、細胞凋亡和生長、噬菌體組裝、蛋白質的還原和修復信號傳導等生理過程產生影響和調節。
  8. Levels of fasting blood glucose and 24h urinary microcontent of albumin 24 h malb were determined dynamically ; the serum glycosyl hemoglobin hba1c was determined after the last medication ; the ultrastructural changes of kidney were observed by transmission electron microscope ; the expressions of collagen, fibronctin, laminin ln, and the ecm metabolism influencing factors, including mmp2, tissue inhibitor of metalloproteinase timp2, transfer growth factor 1 tgf 1 in renal tissue were detected by immunohistological chemistry and image collecting analytical system

    動態檢測各組大鼠空腹血糖fbg 24h尿微量白蛋白24h malb ，末次給藥后測定大鼠血漿糖化血紅蛋白hba1c透射電鏡觀察各組大鼠腎臟超微結構改變，應用免疫組化技術及圖像採集分析系統測定各組大鼠腎臟組織中型膠原c纖維連接蛋白fn層粘連蛋白ln的表達，測定影響ecm代謝的基質金屬蛋白酶2 mmp2基質金屬蛋白酶抑制劑2 timp2及轉化生長因子1 tgf 1的表達。
  9. Nitrate is converted to ammonium by nitrate reductase and ammonium is then incorporated into glutamine and gluamate, either by the glutamine synthase - glutamate synthase ( gs - gogat ) pathway or by glutamate dehydrogenase ( gdh )

    硝酸鹽在硝酸還原酶作用下被轉化為銨，接著所產生的銨在谷氨酰胺合成酶-谷氨酸合酶（ gs - gogat ）或谷氨酸脫氫酶（ gdh ）的作用下與谷氨酰胺和谷氨酸結合。
  10. Detection method of glyphosate oxidoreductase gene from genetically modified roundup - ready rapeseed

    轉基因抗草甘膦油菜籽中草甘膦氧化還原酶基因的檢測方法研究
  11. The expression vector pse380 - / iy / was constructed and transformed into e. coli dh5a, expressing hyl gene by adding iptg into the broth. the expression of hyl gene showed a 120kda protein band on sds - page gel and was found to have capability to degrade ha molecules derived from a microorganism dissolved in 0. 1 m acetate buffer solution ( ph4. 0 )

    經轉化大腸桿菌dh5a和iptg誘導表達後用sds - page電泳分析，獲得一條約120kda的表達條帶； iptg誘導表達后提取原生質膜測定透明質酸分解酶活力，表明該hyl片段的產物能夠在體外分解細菌來源的ha 。採用兩種策略滅活hyl基因。
  12. In the first part of the present work, the expression changes of angiotensinogen ( agt ) and angiotensin - converting enzyme ( ace ) as well as its time course characteristics were investigated by rt - pcr, in situ hybridization and western blotting. we also prepared the polyclonal antiserum against agt for the following work

    故本工作應用rt - pcr和原位雜交、 western印跡分析等方法對模擬失重大鼠不同部位動脈血管血管緊張素原（ angiotensinogen ， agt ）及血管緊張素轉化酶（ angiotensin - convertingenzyme ， ace ）基因表達變化的時程特徵進行了觀察，並為后續工作制備了抗agt多克隆抗血清。
  13. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  14. To prepare the wild type mbl in prokaryotic system, a pair of primers was designed and synthesized, and was used to amplify mbl gene from the recombinant vector pgem - mbl that contans wild type mbl cdna. a recombinant prokaryotic expression vector, pet28 - mbl, was constructed by inserted the mbl gene into plasmid pet28 ( b ), and after transfected it into ecoli bl21 ( de3 ) and induced with iptg, recombinant mbl protein was expressed successfully

    本實驗另選用了原核表達質粒pet28 （ b ） ，根據已構建好的含有mbl野生型基因的t載體pgem - mbl ，設計一對引物， pcr擴增mbl基因，凝膠回收，雙酶切pcr產物和pet28 （ b ）質粒， t4連接酶連接，轉化大腸桿菌dhsa ，氨芋選擇培養挑取克隆鑒定。
  15. The rat 20a - hydroxysteroid dehydrogenase ( 20ahsd ) belongs to a family member of aldo - ketoreductases used nadph as a cofactor and principally converts progesterone to 20a - hydroxyprogesterone as a lower or non - active hormone. thus 20ohsd acts as a molecular switch and makes the potent progesterone hormone into an inactive potent metabolite

    大鼠20 -羥類固醇脫氫酶（ 20 - hydroxysteroiddehydrogenase ， 20 hsd ）屬于醛酮氧化還原酶系成員，在大鼠卵巢中以nadph為輔酶將孕酮轉變為低或無活性的20 -羥孕酮。
  16. Selected one of the 14 strains - s93, s93 dna was digested partially with sau3a i and 2 ~ 3kb fragments were collected and inserted into puc 18, then transformed into dh5 a. filtering the clone with hybridization in situ, a 1 kb frament clone has been cloned

    使用sau3ai對基因組dna進行不完全酶切，回收2 3kb片段，與puc18質粒連接轉化大腸桿菌，利用地高辛標記探針，使用菌落原位雜交篩選轉化子；篩選到包含有約1kb外源片段的轉化子。
  17. This study was to investigate the effects of sulfur dioxide inhalation at different concentrations on some glutathione - related enzymes such as glutathione s - transferase ( gst ), glucose 6 - phosphate dehydrogenase ( g6pd ) and glutathione reductase ( gred ) in brain, lung, heart, liver, kidney and spleen of mice by the technology of biochemical toxicology. the results were showed as follows, so2 exposure at different concentrations caused the changes of glutathione redox system. moreover, the activities of antioxidative enzymes and the contents of reduced glutathione ( gsh ) were decreased significantly in different tissues at higher concentrations of soa

    本研究利用生化毒理學技術研究了不同濃度二氧化硫吸入（ 22 2mg m ~ 3 ， 64 3mg m ~ 3 ， 148 23mg m ~ 3 ）對純系昆明小鼠腦、肺、心、肝、腎、脾六種組織的谷胱甘肽還原酶（ glutathionereductase ， gred ） 、谷胱甘肽硫轉移酶（ glutathiones - transferase ， gst ）和葡萄糖- 6 -磷酸脫氫酶（ glucose6 - phosphmedehydrogenase ， g6pd ）活性的影響，結果表明so _ 2吸入使小鼠不同組織的谷胱甘肽氧化還原系統發生了改變，表現為隨著so _ 2吸入濃度的增加，該系統中的抗氧化酶活性的顯著變化和抗氧化物質水平的顯著降低，且存在著組織差異性。
  18. A 1. 7kb fragment encoding ge of prv fa strain was obtained by pcr from plasmid ppge templated using a pair of the designed primers containing ecori and bamhi ' sites. the ge gene fragment cutted with ecori and bamhi was inserted into the expression plasmid pbv220 including these two endonuclease sites for constructing the recombinant plasmid pbvge. strain dh5a of e. coli contain pbvge was induced at 42 for 4 - 6hr after incubation with vigorous shaking at 30 for 3hr or so

    以質粒ppgedna為模板， pcr擴增出1 . 7kb的ge基因完整片段，將擴增產物以ecori和bamhi雙酶切后，插入原核表達載體pbv220的p _ rp _ l啟動子下游的ecori和bamhi位點間，得到重組表達質粒pbvge ，轉化了pbvge的大腸桿菌dh5a經溫敏誘導表達后，用sds - page和western - blot ，以及瓊脂雙擴散來檢測，結果表明prvfa株ge基因在原核載體上得到高效表達，表達產物約占總蛋白的17 。
  19. At the same time, vp4 gene was mutated in a certain point by pcr. the plant expression vector : pbi121 was constructed and then transformed to agrobacteriutn tumefaciens eha105 directly. a. tumefaciens eha105 harboring pbi121 / vp4 was used to transform the boechmeria nivea l. guad according to the leaf disc procedure

    為便於基因操作，對外殼抗原蛋白vp4基因進行適當的修飾和改造：通過引物設計，利用pcr反應，在基因的起始編碼前引入有助於真核生物表達的kozak序列和限制性內切酶位點；使用套疊pcr對vp4基因進行定點突變，以便於將改造后的基因插入pbi121構建植物表達載體，通過直接轉化法，把pbi121 vp4轉入農桿菌eha105 ，構建了農桿菌工程菌。
  20. The pretreating methods, the enzymatic hydrolysis of cellulose and hemicellulose, the breeding of effective strains for fermentation, and the development of the preparation technology for ligncellulose are mainly introduced

    重點介紹了木質纖維素轉化為乙醇的原料預處理方法、纖維素和半纖維素的酶法降解、有效可靠的發酵菌種的選育及木質纖維素乙醇制備工藝的開發。
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