轉導蛋白 的英文怎麼說

中文拼音 [zhuǎndǎodànbái]
轉導蛋白 英文
transducin
  • 轉 : 轉構詞成分。
  • 導 : 動詞1. （引導） lead; guide 2. （傳導） transmit; conduct 3. （開導） instruct; teach; give guidance to
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. The b lymphocyte stim lator ( blys ) also known as baff, tall - 1, thank, ztnf4, is the most recent addition to the tumor necrosis factor family ( tnf ) ligands. blys induces both in vivo and in vitro b cell proliferation, differentiation and immunoglob lin secretion. meanwhile, it also strongly suppresses the growth of tumor cell lines. the over - production of blys is associated with the development of certain autoimmune disease, such as systemic lupus erythaematosus ( sle ), rheumatoid arthritis, myasthenia gravis, sj greh ' s syndrome

    人b淋巴細胞刺激因子（ hblys ）是1999年發現的腫瘤壞死因子超家族成員。最早發現其可誘導腫瘤細胞凋亡，之後發現它是一個強有力的b細胞共刺激因子，它在體外及轉基因動物體內可明顯刺激b細胞的生長，免疫球蛋白分泌，並導致動物自身免疫性改變。
  3. Finally 4 right clones was obtained from 864 transformants by pcr detection. sequencing analysis showed that the hsa gene have been introduced into the right position of the human a - lactalbumin yac. furthermore, works to optimize the conditions of introducing the recombinant yac into goat f ibroblast via cell fusion was explorded

    經pcr檢測，從864個轉化子中獲得了4個陽性克隆，測序表明，人血清白蛋白基因已正確的導入到人-乳白蛋白基因yac的特定位點上，並獲得了可進行細胞融合的重組yac載體。
  4. But without rdrp transcription were tested. so it is suggested that tm - 22 is a relative to multi - protein transcriptional coactivator. mip204 and pr - la gene expression can be induced in resistant response and do not relate to rdrp gene transcription

    因此，可以認為tm一22基因的編碼蛋白與復合蛋白轉錄因子有關，在抗病反應中能夠誘導五刃滬204和屍尺一ia基因的表達，與rdrp基因的轉錄沒有直接的聯系。
  5. Translocation from endocytic compartments to the cytosol is the essential and rate - limiting step in the intoxication process of most toxins such as ricin, diphtheria toxin, shiga toxin and pseudomonas exotoxin ( pe ). a number of these toxins are transported to trans - golgi network ( tgn ), and in many cases such transport to the tgn is required for the translocation and cytotoxicity. in deed, 5 % of the ricin endocytosed by cells has been shown to reach the tgn

    蓖麻毒素進入細胞的機理不甚明了，一般認為是rtb先與細胞膜受體結合，主要經過受體介導的內吞作用進入吞噬體，然後沿著內體、高爾基體、內質網等逆向分泌途徑，有序地運輸到內質網，最後從內質網轉位進入細胞漿，在胞漿內攻擊核糖體，從而抑制蛋白質的合成，導致細胞死亡。
  6. The effect of metal ions on the conformation transition of silk fibroin

    金屬離子導致的絲素蛋白的構象轉變
  7. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  8. Moreover, they have hi common the localization in the golgi apparatus, the topology of type ii membrane proteins, and the transfer of galactose from udp - galactose onto the c4 - hydroxyl group of glcnac residues

    用iptg誘導表達的獲得- 1 ， 4 - gt與gst （谷胱甘肽轉移酶）的融合蛋白，經westernblot驗證與預想的一致。
  9. The tumor cells transfected with restin are arrested in gl phase and the cell proliferation is inhibited. it implies that restin may be correlated to cell cycle regulation. other data showed that the necdin, a homolog of restin, could suppress cell growth by interacting with transcription factor e2f1 and p53

    而與restin的同源蛋白的necdin ，可以與細胞周期促進蛋白sv40大t抗原、腺病毒eia 、轉錄因子ezfi等相互作用，抑制細胞生長;也可以和轉錄因子p53作用參與生長抑制和抑制p53誘導的細胞凋亡。
  10. By observing the effect of acidity on the uv difference spectra of ehpg or hbed, the spectral characteristics of apoovotransferrin binding to metals and anions at ph 7. 4 show that the reaction of apoovotransferrin with metals is related to deprotonation of the phenolic group of tyrosine of the metal binding sites, while the binding of apoovotransferrin to anions is associated with the protonation of the phenolic group of tyrosine of the anion binding sites

    摘要通過觀察酸度對模擬轉鐵蛋白小分子ehpg和hbed紫外差光譜的影響，結合脫鐵伴清蛋白與金屬離子、陰離子結合的光譜特性，推斷脫鐵伴清蛋白與金屬離子的結合導致金屬離子結合位點處酪氨酸酚羥基的去質子化，而與陰離子的結合導致陰離子結合位點處酪氨酸酚羥基的質子化。
  11. The e. coli strain jm109 was transformed with resultant plasmid pgex - 4t - 1 / 6 - 4. 4. the transformation was induced with iptg, then the total protein from cell extract was analyzed by electrophoresis on a 8 % sds - page in order to validate the gst fusion protein, and the fusion protein is about 90kd

    4 .用工ptg誘導含pgex一4t一1 / 6一4的轉化菌，提取初提物中的總蛋白，進行sds一聚丙烯酞胺凝膠電泳，檢測表達的融合蛋白大小越為gokd 。
  12. Now it has been one of the most important aquatic products in the freshwater cultivation. however, this prawn ca n ' t survive at a water temperature lower than 14c, which has seriously limited its cultivation expanding. in order to obta in a new breed of this prawn with increased cold - resistance, we investigated the cloning of a synthetic gene ( sbwafp ) based on the primary sequence of the mature spruce budworm ( choristoneura fumiferana ) antifreeze protein ( sbwafp ) and the integration of sbwafp into the embryo genomes of giant freshwater prawn by spermatophore - microinjection ( smi ), a sperm - mediated gene transfer technique

    本研究的特色和創新之處在於，針對羅氏沼蝦不耐低溫，但體型相對較大，精莢明顯的特點，首次將目前已知具有最強抗凍活性的雲杉卷葉蛾（ sprucebudworm ， choristoneurafumiferana ）抗凍蛋白（ sbwafp ）基因（ sbwafp ） ，通過精子介導的轉基因技術整合到羅氏沼蝦的胚胎中，以期培育出耐低溫的羅氏沼蝦新品系。
  13. The regeneration system of soybean cytoledon node and agrobacteriunr mediated transformation method is the first selection at present. in the second part of this experiment, the expression vector prok2 containing npt ii and ssnhx1 ( na + / h + antiporter ) gene from suaeda salsa was introduced into soybean cytoledon nodes by gene transformation mediated by agrobacterium tumefaciens, and kanamycin resistant transgenic p lants were obtained by screening in selective condition

    本實驗第二部分通過農桿菌介導法將含npt -和鹽地堿蓬na ~ + h ~ +反向轉運蛋白基因（ ssnhx1 ）的表達載體prok2導入大豆子葉節中，經過含km的篩選培養基連續篩選，獲得了ssnhx1轉基因植株，篩選劑卡那黴素的適宜濃度是50mg . l ~ （ - 1 ） 。
  14. Furthermore, more and more evidence indicate that various rab proteins concertedly and concordantly regulate the cytosolic protein transport. at present, more than 40 rab gtpases were found, but according to the human genome analysis, it is estimated that about 60 rab ests exist totally

    Rab蛋白和另浙江大學碩士學位論文單核細胞系分化相關小g蛋白rab7b一種調節蛋白snare （ n一ethyl一maleimidesensitivefactor一solublensfattachmentprotein ）在介導蛋白轉運上可以說是一對功能互補的調節蛋白，只是側重點不同。
  15. Immune effect induced by s. japonicum dna vaccine with different vectors in mice

    同型信號轉導蛋白誘導小鼠保護性免疫的研究
  16. The hnadcs ( human na + / dicarboxylate cotransporter 3 ) mediates the transport of krebs cycle intermediates, such as succinate, citrate and a - ketoglutarate, across the plasma membrane of many epithelial cells

    人鈉二羧酸協同轉運蛋白3 （ humanna ~ + dicarboxylatecotransporter3 ， hnadc3 ）基因的編碼產物是位於多種上皮細胞膜表面的一類介導琥珀酸、枸櫞酸及-酮戊二酸等三羧酸循環中間產物轉運的蛋白。
  17. About 25 salt - induced proteins were identified by maldi - tof / ms, and finally the function of 17 induced proteins were determined, including heat - shock proteins ( groes, clp ). abc transporter, rna polymerase ( p - subunit ) and enzymes involved in signal transduction and metabolism. meanwhile, 84 proteins were idifferentially expressed after salt shock 5 min and 50 min in late exponential phase

    應用maldi - tof ms對30個鹽脅迫蛋白進行分析，已初步確定17個誘導蛋白的功能，其中包括與鹽脅迫相關的熱激蛋白（ groes 、 clp ） 、與代謝途徑和信號傳導等有關的酶，以及abc轉運蛋白、轉錄調節蛋白、 rna聚合酶的亞基等。
  18. Abc transporter, the elongation factors ( ef - tu ) and the other enzymes involved electron transduction and metabolism. finally, comparing long - termed salt stress with transient salt shock, there are 12 proteins induced in common, which means different stress regulatory mechanisms are inextricably linked to each other

    應用maldi - tof ms分析，已初步確定25個誘導蛋白的功能，其中包括親和溶質脯氨酸合成必需的酶、 abc轉運蛋白、熱激蛋白（ hsp60 ） 、 dnak及蛋白合成的伸長因子ef - tu 、與代謝途徑、信號傳導和電子傳遞系統有關的酶。
  19. In addition, the well retained stability and integrity of cell membrane of boea leaves might also be an important mechanism which make them resurrect well. by using mrna differential display, 5 desiccation sensitive cdnas, 52 desiccation - induced cdnas, 21 up - regulated cdnas, 14 down - regulated cdnas and 16 phosphate induced cdnas were obtained. the cloning, sequencing, homological blasting and northern blotting results of 5 desiccation - induced cdnas and 3 phosphate induced cdnas implied that signal transduction induced by desiccation, regulatory gene cascades and functional genes such as g protein, protein kinase, vp3 - and mad3 - like genes might be involved in dehydration in the resurrection plant boea hygrometrica

    對其中5個脫水特異誘導表達牛耳草光合作廠j的脫水保護和復甦機理的cdna （包括可能與復甦能力有關的cdna ）和3個磷酸鹽處理誘導表達的cdna進行克險測序、同源性探測和northern雜交檢測表明，牛耳草脫水過程中誘導表達的基因可能涉及到脫水脅迫的信號轉導「蛋白、蛋白激酶等） 、調節基因的級聯作用（ vp3 ， mad3樣基因等） 、結構基因產物調節細胞結構（包括細胞質膜）在脫水脅迫中的穩定性等。
  20. Studying the significant role of transporters in drug blood - brain barrier can provide deep analysis of the drug kinetics in the blood - brain barrier transport with effective regulation and helpful for understanding the interaction between the transporters and substrate ; and furthermore with guidance to the research and development of drugs including brain - targeting and nonbrain - targeting

    對轉運蛋白在藥物血腦屏障轉運中的重要作用進行研究，可深入地解析藥物在血腦屏障上的動態並更有效地調控藥物轉運行為，有助於了解血腦屏障上轉運蛋白與底物藥物的相互作用，指導腦靶向與非腦靶向藥物的研發設計。
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