轉片桿 的英文怎麼說

中文拼音 [zhuǎnpiāngǎn]
轉片桿 英文
crank blade rocket
  • 轉 : 轉構詞成分。
  • 片 : 片構詞成分。
  • 桿 : 桿名詞（桿子） pole; staff
  1. The practical issue of drum brake with oversized overall stroke was resolved by experiments, and it indicates that major effect factors of air chamber overall stroke, which are the drum - shoe clearance, the elastic modulus of lining, the torsion deflection of cam shaft

    研究結果表明，氣室推桿總行程過大的主要影響因素為制動蹄與制動鼓之間的間隙、摩擦片的彈性模量、凸輪軸的扭轉變形。
  2. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  3. Obtaining transgenic male sterile tobacco in order to prove that hsp70 antisense cdna can lead to male sterility, with plasmid 3301 + 650, 3301 + 651 we transformed 207 aspetic tobacco leaves by genegun bombarding and agrobacterium mediation ( 109 by genegun bombarding, 98 by agrobacterium ). by cultivating them in blotting media containing basta 0. 4 mg / 1, we get 181 resistant leaves ( 98 by genegun bombarding, 88 by agrobacterium mediating )

    獲得轉基因雄性不育煙草為了證實hsp70反義cdna能創造雄性不育，我們將3301 + 650和3301 + 651質粒用基因槍和農桿菌介導法轉化煙草無菌發芽的葉片，共207片（基因槍109片，農桿菌98片） 。在含basta0 . 4mg l的篩選培養基上進行篩選，得到抗性葉片181片（基因槍93片，農桿菌88片） 。
  4. Regaining new stable equilibrium he rose uninjured though concussed by the impact, raised the latch of the area door by the exertion of force at its freely moving flange and by leverage of the first kind applied at its fulcrum gained retarded access to the kitchen through the subadjacent scullery, ignited a lucifer match by friction, set free inflammable coal gas by turning on the ventcock, lit a high flame which, by regulating, he reduced to quiescent candescence and lit finally a portable candle

    他重新獲得了穩定均衡，盡管因猛烈撞擊而受震蕩，卻沒有負外傷就站了起來。他使勁扳院門搭扣的那個活動金屬片，憑著加在這一支軸上的初級杠桿的作用，把搭扣摘開，穿過緊挨著廚房地下的碗碟洗滌槽，繞道走進廚房。他擦著了一根安全火柴，轉動煤氣開關，放出可燃性的煤氣。
  5. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  6. The dna sequence had a complete orf ( open reading frame ), which coded a protein of 479 amino acid residues. the protein sequence of enolase which contained the conserved domain, was homologue to enolase of other organisms. it showed 83 % ideaity and 89 % similarity compared to the enolase in chlamydomonas reinhardtii

    並將其中的一個gus基因用目的片段烯醇酶基因替換，構建了可以在植物中高效表達的載體pcambia2301g一enolase ，成功地將其轉入根癌農桿菌eha105中，為下一步進行轉基因植物的研究作準備。
  7. The materials as explant in transformation come from birch leaf, stem segment and leaf stalk, and the spider toxin gene was used as foreign gene for this transformation experiment. it showed that the best explant was the big leaf, on which the transformation frequency was 22 %. by gus detection, there were 43 percent of the plants with kanamycin resistance, and 100 percent of positive result, by pcr amplification, was gotten from random sampling

    利用雙元載體的根癌農桿菌lba4404菌株（ agrobacteriumtumefaciens ） ，含質粒pyhy （目的基因及npt 、 gus基因） ，對白樺試管苗莖段，葉柄，葉片三種外植體進行侵染，結果表明：大葉片生長勢強，為轉基因的最優外植體，轉化率能夠達到22 。
  8. The result showed that the homology rate of pila gene among the 5 avian pathogenic e. coli strains tested and one human e. coli were from 89. 8 % to 91. 1 %, and the homology rate of amino acid were from 88. 5 % to 91. 8 %. the homology rate of pila gene sequence among 5 avian pathogenic e. coli strains tested and avian pathogenic e. coli reported ( serotype o1, o2, o78 ) were from 87. 8 % to 90. 2 %, and the homology rate of amino acid were from 84. 6 % to 91. 2 %. there had homology in avian pathogenic e. coli. there had some common antigen side in type 1 pili of avian pathogenic e. coli

    結果表明：運用msha法檢測1型菌毛的檢出率為80 （ 36 45 ） ， pcr法的檢出率為95 . 5 （ 43 45 ） ， pcr方法用於1型菌毛的檢測比msha更加敏感、快速、特異性強；選擇5株優勢血清型雞源致病性大腸桿菌代表株（ o _ （ 89 ） ， o _ （ 119 ） ， o _ （ 141 ） ， o _ （ 127 ） ）的1型菌毛pila基因的pcr擴增片段經純化后，分別定向克隆到puc18質粒的多克隆位點，構建了含有目的基因片段的克隆質粒，並轉化到dh5株大腸桿菌載體菌中，篩選獲得陽性克隆菌株。
  9. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  10. Leaves of tobacco ( nicotiana tabacum ) were wounded, infected by agrobacterium tumefaciens strain lba4404 and gv3101 : pmp90 harboring expression vector pbgsb and pbglsb and co - cultured for 3 days in darkness on the culture medium ( ms + 0. 5mg / l 6 - ba + 0. 7 % agor ph 5. 7 )

    4以煙草（品種sr ）無菌苗葉片為外植體，採用農桿菌浸染的葉盤轉化法，用構建好的植物表達載體對煙草進行遺傳轉化。外植體置於無抗生素的誘芽培養基（ ms 0
  11. Transgenic plants were comfirmed by molecular determination including pcr, southern blot and rt - pcr. 4. functional analysis and iron content of transgenic plant crude proteins of transgenic plant were extracted from leaves, and then used to test its antibiotic activities

    4 .轉基因植株抗菌能力加強，鐵含量得到提高提取轉基因植株葉片粗蛋白，以大腸桿菌dhsq和酵母菌ahiog為試驗靶標，側定了轉基因植株粗蛋白的抑菌活性。
  12. The experimental results show that the wave shaper can control the moving direction of the rod fragments after the warheads exploded and ensure the integrity rate of the rod and that there is a close relationship between the flight attitude and the positioned angle, which is mainly reflected by the rotation velocity of the rod

    試驗結果表明：採用波形控制器可以較好地控制戰斗部爆炸后桿形破片的飛散方向，以及保證桿條的完整率；桿條的飛行姿態與放置角有密切關系，主要通過桿條旋轉速度表現出來。
  13. The expression vector pse380 - / iy / was constructed and transformed into e. coli dh5a, expressing hyl gene by adding iptg into the broth. the expression of hyl gene showed a 120kda protein band on sds - page gel and was found to have capability to degrade ha molecules derived from a microorganism dissolved in 0. 1 m acetate buffer solution ( ph4. 0 )

    經轉化大腸桿菌dh5a和iptg誘導表達後用sds - page電泳分析，獲得一條約120kda的表達條帶； iptg誘導表達后提取原生質膜測定透明質酸分解酶活力，表明該hyl片段的產物能夠在體外分解細菌來源的ha 。採用兩種策略滅活hyl基因。
  14. Locking the rotation of the laminar coupling ( 2 ) with the reaction lever 99370317 ( 1 ) and using a suitable wrench unscrew the retaining screws ( 3 ), remove the laminar coupling and the flange

    利用止動桿99370317 （ 1 ）鎖定迭片式聯軸節（ 2 ）不讓其轉動，並且使用適用的扳手鬆開固定螺釘（ 3 ） ，將迭片式聯軸節和法蘭卸下。
  15. According to the high precision and rotation stability requirement of negative scan unit of cylinder photography typesetting machine, the three levels of cylindrical gears transmission separately, the two - stage cylindrical gears - worm drive, and the worm drive was chosen through calculating its velocity ratio, the checking transmission precision, carrying on the movement precision analysis to the sweep unit, and computing overall transmission error

    摘要針對圓筒型照相排版機底片掃描裝置要求傳動精度高，轉動平穩的特點，分別選擇三級圓柱齒輪傳動，雙級圓柱齒輪蝸桿傳動，蝸桿傳動3種常見的傳動方式，計算其傳動比，驗算傳動精度，對掃描裝置進行運動精度分析，計算總體傳動誤差。
  16. Boring bars for indexable inserts. dimensions

    帶可轉位鑲嵌刀片用鏜桿.尺寸
  17. Cells were then harvested by centrifugation and the pellet was resuspended in phosphate - buffered saline ( pbs ) containing 5 mm edta. after sonication, debris was removed by centrifugation at 10000 x g for 15 min at 4

    挑取轉化后的大腸桿菌提取質粒， ecori和hindln酶切質粒進行鑒定，瓊脂搪電泳顯示含有大約800kb的目的片段。
  18. Taken in the 1930 ', these is a valuable picture of roasting a duck. and now it is collected by the beijing capital museum

    這是一張六十年前烤鴨師傅挑桿轉動烤鴨的珍貴照片，現藏於北京首都博物館。
  19. Based on a 3. 1kb pst i fragment of genomic dna of a wild s. avermitilis, a 1. 5 kb apramycin resistance fragment was inserted into sph i site of avec gene in the 3. 1 kb fragment, then a recombinant plasmid pc05 was obtained by introducing above inactivated avec fragment into mcs region of phjl401. competent cells of et12567 were transformed by recombinant plasmid pid03 and pc05 respectively

    以含有avec基因的3 . 1kb基因組dnapsti片段為基礎，將1 . 5kb的安普黴素抗性基因片段插入到avec基因中的sphi酶切位點，再將此插入失活的avec基因片段連接到具有接合轉移功能（含有orit基因）的鏈黴菌-大腸桿菌穿梭質粒phjl401的多克隆位點區，由此得到重組質粒pc05 。
  20. Then, the plasmid was transformed into jm109. the full length pstvd cdna recombinant plasmid was further identified by restriction mapping analysis and the nucleotide sequence of cdna cloned in pmd 18 - t vector was analyzed with ab1 377 dna sequence. test showed that the cdna of this chinese pstvd isolate was the same as pstvd - kf - 6 mild " type " isolate which originated from naturally infected potatoes in field of cornell university potato breeding program

    利用rt - pgr技術，設計一對引物，對田間採集的經鑒定含pstvd的陽性樣品進行全序列擴增，並將所得產物純化回收，連接到pmd18t - vector中，並轉化至大腸桿菌jm109中，挑選白斑進行雙酶切（ saci / ecorv ）鑒定證明插入片段為359bp大小，進行序列測定，所得克隆基因為pstvd全序列負鏈，大小為359bp 。
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