轉甲基酶 的英文怎麼說
中文拼音 [zhuǎnjiǎjī]
轉甲基酶
英文
methyl kinase-
2 how dose betaine affect dna - methyltransferase
甜菜堿如何影響dna甲基轉移酶的活性Catechol o methyl transferase, comt
兒茶酚氧位甲基轉移酶Polymorphism of catechol - o - methyltransferase gene in relation to the risk of endometrial cancer
甲基轉移酶基因多態性與子宮內膜癌遺傳易感性的關系Other : the dyewood is known as the suppression histidine decarboxylase, the catecha phenol - o - methyl shift enzyme action
其它:染料木素有抑制組氨酸脫羧酶，兒茶酚- o -甲基轉移酶作用In addition, it was also found that this protein shares limited but significant homology with the sam - dependent methyltransferase of mesorhizobium sp. bnc1 ( 32 % similarity ), and the similarity of its 303 - 362 region to the 160 - 220 domains of l11 methyltransferases of e. coli ( prma ) is 41 %. it is suggested that methylation of l11 resulted in effects of noea on nodulation of 042bm
發現noea與中慢生根瘤菌（ mesorhizobiumsp . ） bnc1可能的sam -依賴性的甲基轉移酶相似性為32 ，而其303 - 362區域與大腸桿菌（ escherichiacoli ）的核糖體50s亞基的l11蛋白甲基轉移酶（ prma ）的160 - 220結構域的相似性達到41 。Transformtion of soybean with - tmt from brassica oleraea for - tocopherol elevtion
生育酚甲基轉移酶基因大豆的獲得When both genes were co - expressed in e. coli, the activity of ppsa varied from 2. 1 - 9. 1 fold comparing to control, but the activity of tkta was relatively stable ( 3. 9 - 4. 5 fold ). whatever the two genes were expressed respectively or cooperatively, both could promote the production of dahp, the first intermediate of the common aromatic pathway, but co - expression was more effective on forming dahp and screened ppt - and ptp - as more effective. the results demonstrate that co - expression of ppsa and tkta can improve the production of dahp, and what ' s more, when multigenes co - expressed, the recombinant which has coordinated enzymes activity is optimum
莽草酸途徑的最優化和整體調控基因csra的敲除正是上述改變的分子基礎，同時也為三種芳香族氨基酸的基因工程菌的構建打下了基礎; 7 .在國內外首次實現了共同途徑限制性底物關鍵酶ppsa刁無『及arog與分支途徑關鍵酶基因phea的串聯高效表達，所構建的重組質粒ptga ，其ppsa 、 tkta 、 arog 、 cm和pd的酶活分別比對照提高了3 、 2 、 2 ， 5 、 4 、 2 . 3倍，且其酶活比較協調一致; 8 .將ptga導入到篩選的基因敲除和基因替換菌株大腸桿菌31884 c甲b中，搖瓶發酵證實比以往所構建的基因工程菌株具有較高的phe產量和糖轉化率率，分別為0 . 448 %和22 . 4 % 。The transfermants with highest resistance to g418 ( 4 g / l in ypd ) were screened to study their expression level in 150 ml shaking flask. having been induced with methanol for 36 hours, the target enzyme could be examined in the supernatant by measuring amidolytic activity
首次將大連蛇島賅蛇毒類凝血酶成熟基回克隆到表達載體ppicgk中，經電激轉化至畢氏酵母菌株gs15中，再經甲醇誘導，在150ml搖瓶畔1獲得細胞外分泌表達產物。It was confirmed that the key enzyme of coq10 synthesis is p - hydroxybenzoate polyprenyltransferase, encoded by gene ubia in e. coli and lacks specificity to the aggregation length of its substrate ( polyisoprenyl pyrophosphate ). if gene ubia can be introduced into psb and is high expression in psb, a recombinant strain producing coq10 could be obtained. consequently, the productive cost will decrease sharply
業已證實coq _ （ 10 ）生物合成的關鍵酶為對羥基苯甲酸聚異戊二烯焦磷酸轉移酶（在大腸桿菌中該酶由ubia基因編碼） ，該類酶對底物聚異戊二烯焦磷酸（ ppp ）的聚合長度並無特殊要求。In bioconversion of a d - amino acid and its derivative, the substrate, 5 ' - monosubstituted hydantoin is firstly transferred into an intermediate, d - carbamyl amino acid by d - hydantoinase, and in turn into an optically active d - amino acid by either d - carbamoylase or chemical, nano2. nowadays, the bioconversion of d - amino acids has been industrialized by enzymes, so called one - step process
D -型氨基酸及其衍生物的生物轉化，經歷兩個反應歷程，首先是在海因酶的作用下，催化5 -單替代海因形成氨甲酰類氨基酸中間物，後者再在酶或化學的作用下，生成相應的光學活性氨基酸或其衍生物。A. niger m - l which was screened in our laboratory produced a strongly a - transglucosidase. in this paper, studies on the fermentation conditions, purification and characterization of a - transglucosidase and its necessary groups was carried out in this dissertation. the main reports were as following : the fermentation conditions in shaking flasks were investigated by the method of single - factor analysis, the suitable main medium was achieved : which contained 4 % a, 2 % b and 1 % g ; the a. niger m - l was inoculated into 100ml medium in flask, shaking in 33 c at 140r / min for four days, with initial ph6. 5 and 6 % inocula volume ; adding 0. 1 mmol / l methyl a - d - glucopyranoside had inductive effect on enzyme formation, the a - transglucosidase activity amounted to 296. 05u / ml
本研究以黑麴黴m - 1為出發菌株，對其-葡萄糖轉苷酶的產酶影響因素、純化、酶學性質以及必需基團進行系統的研究，結果如下：通過對影響黑麴黴m - 1產-葡萄糖轉苷酶的單因素分析，得液態發酵生產-葡萄糖轉苷酶的最適產酶條件為： 4 a ， 2 b和1 g ；在33 ，起始ph值為6 . 5 ，轉速為140r min ，接種量為6 ，裝液量100ml條件下，發酵4 . 0d ，酶活力達296 . 05u ml ，添加0 . 1mmol l的酶作用底物甲基- - d -葡萄糖苷對產酶的誘導作用最大。The objective of this research is to transform the cloned phytase gene into pichia pastoris in order to obtain high - yield phytase - producing strain and to optimize the engineered yeast media recipes for the scale - up production of phytase. main results of this research are as follow : 1, xba i - linized recombinant plasmid ppic9k - phya was transformed into pichia pastoris by gene pulser. 98 positive transformants showing measurable phytase activities were screened on md plates and ypd plates containing g418. they all grew quickly on both md plates and mm plates, which proved their phenotypes of methanol utilization were mut +
主要實驗結查如下：西南農業大學碩士學位論文1 、用乃ai酶切攜帶植酸酶基因表達片段的重組質粒ppicgk夕句） a ，回收dna ，用genepulser電擊轉化畢赤酵母，塗布md平板，又用含不同濃度g418的ypd平板進行抗性篩選，得到98個可檢測到植酸酶活力的陽性轉化子，它們在md 、 mm平板上均表現快速生長，說明其甲醇利用表型是mut干。In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals
首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。As far as the enzymatic activity is concerned, on the one hand, strain yz - ii6 has higher d - hydantoinase activity and lower d - carbamoylase activity so as not to be suitable for one - step bioconversion of d - amino acids, on the other hand, the higher hydantoinase activity, engineered strain in particular, may be convenient to be as a biocatalyst to produce n - carbamyl d - amino acids which hard to find in the markets
Yz - 6菌具有較高的海因酶活性，但n -氨甲酰基d -氨基酸酰氨水解酶活性很低，仍不適合用於d -型氨基酸生產工藝中的一步法轉化。另一方面，含海因酶基因的人工菌株不但酶活性高，也排除了天然菌株轉化生產d -型氨基酸過程中的一些副產物。Expression of o6 - methylguanine - dna methyltransferase in breast cancer
甲基轉移酶的表達Preparation and clinical application of the monoclonal antibodies against the human o6 - methylguanine - dna methyltransferase
抗人甲基轉移酶單克隆抗體的制備及臨床應用研究分享友人