轉糖基酶 的英文怎麼說
中文拼音 [zhuǎntángjī]
轉糖基酶
英文
glycosyl transferase-
Udp galactose : n acetyl glucosamine galactosyl transferase
乙酰氨基葡萄糖半乳糖基轉移酶Udp gal nac : gm3 n acetyl glucosaminyl transferase
乙酰氨基葡萄糖轉移酶In this study, the stem segments of new shoot with axillary buds of well - growth tetraploid black locust trees were used as explants. the effects of different basic mediums, different hormone kinds and their concentrations ratios, different sucrose concentrations on calli induction, buds differentiation and rooting in the process of establishment of high frequency regeneration system of tetraploid black locust were studied. on the base of high frequency regeneration system, the effects of various factors on transformation efficiency of badh mediated by agrobacterium tumefaciens were discussed in the light of gus histochemical assays
本實驗首先以生長良好的四倍體刺槐優株上當年生新梢的帶腋芽莖段為外植體,研究了在四倍體刺槐高頻再生體系的建立過程中不同基本培養基、不同激素濃度及其配比、不同蔗糖濃度對愈傷組織的誘導、芽的分化及生根的影響;然後在得到高頻再生體系的基礎上,通過農桿菌介導法轉化甜菜堿醛脫氫酶( badh )基因,以gus染色組織分析為依據探討了影響轉化效率的各種因素,建立了高效、可重復的基因轉化體系,為四倍體刺槐目的基因的導入打下了基礎。Among the genes, there were genes directly related to liver regeneration : fetuin, cathepsin ; close related to liver function : cytoplamic aspartate aminotranferase, gutathion sulfur transferase ; related to substance and energy metabolism : atp synthetase, ribosomal protein, and related to stress response : haptoglobin, transferrin
這些基因中有和肝再生有直接關系的如:胎球蛋白、組織蛋白酶;和肝臟功能密切相關的如:胞質天冬氨酸轉氨酶、谷胱甘肽硫轉移酶;與物質能量代謝有關的如: atp合成酶、核糖體蛋白;以及與急相反應有關的如:觸珠蛋白、轉鐵蛋白。Researches of schistosomiasis vaccines have gone more than 60 years, approximately including from the stages of dead vaccine and live vaccine ( irradiated attenuated cercariae vaccine ) to gene engineered vaccine, etc. many different forms of vaccines have been tested in animal models, including gluthathione s - transferase, paramyosin, irv - 5, triose phosphate isomerase, sm23, fatty acid binding protein ; which were considered promising by who / tdr. but none of them steadily accomplished the pre - set target level of 40 % protection. in order to enhance the protective capacity further, it is essential to develop novel vaccine antigens and / or vaccine adjuvants
血吸蟲病疫苗研究已有60多年的歷史,大致經歷了死疫苗、活疫苗(照射致弱尾蚴疫苗)和基因工程疫苗等研究階段,產生了一些who / tdr推薦認為很有希望的疫苗候選分子,如谷胱甘肽- s -轉移酶( gst ) 、副肌球蛋白( sm97 ) 、照射致弱疫苗抗原5 ( irv - 5 ) 、磷酸丙糖異構酶( tpi ) 、曼氏血吸蟲膜內在蛋白( sm23 )和脂肪酸結合蛋白( fabp , sm14 )等,但其對宿主的保護作用均不甚理想,未能穩定地達到40或以上的保護力水平,因此有必要繼續尋找新的疫苗抗原分子和/或疫苗佐劑,進一步提高其保護力。Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed
本實驗以河套蜜瓜果肉基因組dna為模板,用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增,得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上,篩選反向克隆,然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游,構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上,通過花粉管通道法轉化河套蜜瓜,共獲76顆瓜,並進行了硬度和含糖量的分析。Performance of the chinese hamster ovary cell hypoxanthine guanine phosphoribosyl transferase gene mutation assay
中國倉鼠卵巢細胞次黃質鳥嘌呤轉磷酸核糖基酶基因變異鑒定的操作The results suggested that the enzymatic activity of invertase from high to low was yh 、 yn 、 yf, and the amino acid sequence of the three strains were completely identical
得出3株高糖酵母在不同生長階段的蔗糖轉換酶酶活由高到低依次為yh 、 yn 、 yf ,而其蔗糖轉換酶的氨基酸序列卻完全相同。Many glycoproteins of lower and higher eucaryotes are attached to the plasma membrane by means of a glycosylphosphatidylinositol ( gpi ). gpi - anchored proteins are synthesized on membrane - bound ribosomes. upon translocation of the pro - protein across the endoplasmic reticulum membrane, gpi : protein transamidase ( gp1t ) recognize and removes the carboxy terminal gpi signal sequence and attaches a gpi molecule to the newly exposed carboxy terminal amino acid
Gpi化前體蛋白在依附於膜的核糖體上合成,當其易位穿過內質網( er )膜后,被gpi :蛋白質轉酰胺基酶( gpit )識別, gpit在移走其羧基端gpi信號序列的同時將gpi分子連接至新生成的氨基酸位點上。In the presented study, using h7721 human hepatocarcinoma cell line transfected with sense or antisense cdna of gnt - v, the effects of gnt - v on signal transduction an d its mechanism as well as the alteration of gene expression were investigated. we expected to elucidate whether and how the transfected glycosyltransferase modulate the cell signal transduction and gene expression
本文以穩定轉染gnt - v正義或反義cdna質粒的h7721人肝癌細胞為材料,從下列四個方面對gnt - v影響信號轉導及其機制以及引起基因表達的變化進行了研究,企圖闡明糖基轉移酶轉染是怎樣調節細胞信號轉導的Fos contain mixture of gf2, gf3 and gf4 sugars ( where g = glucose molecule and f = fructose molecule ) and a dp ( “ degree of polymerization ” ) of 3 - 5 ( “ neosugar ” ), are not naturally - occurring but are enzymatically synthetized from sucrose by action of an enzyme from the fungus aspergillus niger
詳細說明:是以蔗糖做底物,採用呋喃果糖苷酶轉果糖基作用,在蔗糖分子上以( 1 2 )糖苷鍵上與1 - 3個果糖分子結合,形成的蔗果三糖( gf2 ) 、蔗果四糖( gf3 ) 、蔗果五糖( gf4 )屬于果糖和蔗糖構成的直鏈雜低聚糖,在形成的產物中還有果糖、葡萄糖和未反應完全的底物蔗糖,採用色譜法除去單糖和雙糖制得高純度的低聚果糖。This contributes to make clearly position and function of - glc in forming course of tea aroma, substantiate the theory that the aroma of tea forms and also establish the foundation that utilize genetic engineering techniques to improve the cultivar of tea plant, quality of tea and insect - resistance and disease - resistance afterwards. in the paper, the complete cdna squence of - glc of tea was cloned in the tea leaf at the first time
因此,嘗試從茶葉中獲取-葡萄糖苷酶基因的cdna序列,將有助於進一步搞清-葡萄糖苷酶在茶葉花果香氣形成中的地位和作用,充實茶葉香氣形成的理論,也為以後利用基因工程手段改良茶樹品種,提高茶葉品質、抗病蟲能力以及該酶的轉化、調控的研究奠定基礎。Northern analysis revealed that p3galt - l was exclusively expressed in the brain while that p4galt - l was expressed highly in testis, moreover, sperm surface p4galt - l was suggested to play a significant role in signal transduction involving the acrosome reaction in the mouse. in the present study, we mainly detected the developmental expression and function of p - galactosyltransferases in mouse brain and testis. we first characterized the developmental expression of 3galt - l in mouse brain
- 1 , 3 -半乳糖基轉移酶- 1 ( 3galt - 1 )主要在腦中表達,而- 1 , 4 -半乳糖基轉移酶- 1在睪丸中表達較高,且精子細胞表面的- 1 , 4 -半乳糖基轉移酶- 1在精卵結合后的頂體反應中發揮了重要的生理作用。Study on characteristics and thermostability of immobilized fructosyltransferase
固定化果糖基轉移酶的理化性質及穩定性研究The gpa1 gene was obtained via pcr amplification and was cloned in the two - hybrid dna binding domain vector pgbkt7 the combinant plasmid was designated as pgbkt7 - ga. - galactosidase assay indicated that got did not have the property of self - activation. after pgbkt7 - ga was transformed to yeast pj69 - 4a, we transformed arabidopsis vegetative tissue two - hybrid cdna library plasmids to yeast pj69 - 4a containing pgbkt7 - ga
通過pcr擴增得到gpa1基因並將其克隆到雙雜交dna結合域載體pgbkt7中,得到的重組質粒命名為pgbkt7 - g , ?半乳糖苷酶活性鑒定表明g不具有自激活特性,將其轉化到酵母菌pj69 - 4a中,再以此為受體菌轉化擬南芥綠色營養組織cdna文庫質粒。The complex of glycan structure is synthesized by glycosyltransferases and the differential expression of glycans during development is due to the developmental expression and activity of glycosyltransferases
復雜的糖鏈結構是由糖基轉移酶依此加工合成的,糖基轉移酶在發育過程中的表達水平及生物活性決定了某一特定糖鏈在發育中的表達變化。Objective : polypeptide : n - acetylgalactosaminyl transferase is the initiation enzyme catalyzing the linkage of o - glucan chain. recent study shows that o - glucosylation is closely related to molecular recognition, tumor formation, development and metastasis, as well as embryonic development. due to the initial study on function of o - glucosylation in china, this thesis aims to obtain stably expressed pp - galnac - t2 gene clones for further study
目的多肽: n乙酰氨基半乳糖轉移酶是合成o糖鏈的起始酶,而目前的研究認為, o -糖基化與分子及細胞識別、腫瘤的發生發展和轉移以及胚胎發育等功能密切相關。In our experiments, we transformed the grifola frondos ( bacidiomycete ) trehalose synthase gene ( tsase ) into tobacco to make further study to verify the function of the gene
本研究利用擔子菌灰樹花海藻糖合酶基因( tsase )遺傳轉化煙草,以進一步驗證該基因的功能。And then a 378bp acci fragment in pbtk2. 6 was replaced by a fragment containing the immediate early promoter of cytomegalovirus and bovine growth hormone polyadenylation signal derived from pcr3 - uni, a eukaryotic expression plasmid
在此基礎上,進一步用tthlll酶切后,補平插入帶有sv40啟動子的大腸桿菌?半乳糖苷酶報一告基因( lacz ) ,獲得通用轉移載體pltk - uni 。Gene population encoding proteins that involve in plant defensive reactions and adversity resistance, such as p1a5 gene encoding a non - specific lipid - transfer protein, p1b4 gene encoding a raucaffricine - o - p - d - glucosidase and p2d4, encoding a proline - rich protein ; ( 2 ) gene populations with antagonistic effects occurred simultaneously in sprouting bract, e. g.,
如pib4基因編碼的葡萄糖昔酶、 pias基因編碼的植物脂肪轉移蛋白、 pzd4基因編碼的一種富含脯氨酸的蛋白都有參與植物的抗逆、防禦或應激反應的功能八2 )幼嫩苞片中有互為桔抗作用的基因同期表達。分享友人