通透酶 的英文怎麼說
中文拼音 [tōngtòu]
通透酶
英文
permease-
Studies on screening of strains of producing phytase and the conditions of producing phytase the strains of producing phytase could be identified by the hydrolysis bound in differential medium. aspergillus niger an010001 secreting phytase was isolated by screening and second screening. the conditions of producing phytase was studied
植酸酶菌株的篩選及產酶條件的研究本項研究利用植酸酶的菌株能在篩選培養基上形成水解透明圈的特點而進行鑒定,通過初篩和復篩,得到一株產植酸酶較高的黑麴黴( aspergillusniger ) an00101菌株。Methods : hyperosmotic pressure animal model was established by administering 3 % sodium chloride as drinking water to rats or increasing osmotic pressure of the culture medium. osmoregulation positions in the brain, reciprocal projection pathways between the medullary visceral zone ( mvz ) and supraoptic nucleus ( son ) or hypothalamic paraventricular nucleus ( pvn ), oscillation of intracellular calcium in cultured neurons and astrocytes were studied by means of anti - fos, glial fibrillary acidic protein ( gfap ), tyrosine hydroxylase ( th ) or vasopressin ( vp ) multiple imrnunohistochemical staining, immuno - electronic microscope, wga - hrp retrogradely tracing and cell culture methods. results : ( 1 ) fos positive neurons within the mvz, parabrachial nuclei, locus ceruleus, pvn, son, subfomical organ increased markedly
方法:通過給予大鼠飲用3氯化鈉或提高培養基滲透壓濃度的方法復制高滲刺激模型,主要採用抗fos 、膠質原纖維酸性蛋白( gfap )和酪氨酸羥化酶( th ) (或加壓素? vp )免疫組織化學多重染色、免疫電鏡、 wga - hrp束路追蹤結合免疫組織化學多重染色、細胞培養等實驗方法,系統觀察了中樞參與滲透壓反射的調控部位、下丘腦視上核( son )神經元? ast超微結構的變化、延髓內臟帶( mvz )和son及下丘腦室旁核( pvn )之間往返投射通路和神經元的性質及其與ast的關系、培養神經元和ast內鈣波的變化。The page revealed the culture supernatant of the initial strain and the mutant contained different protein bands, which exactly demonstrated at protein level that a. niger j 506 was surely a mutant of a. niger m1. zymogram stained with xylan - remazol brilliant blue for detecting xylanase showed there are three different xylanases in the mutant culture, while two xylanases in initial strain. what is important, the third xylanase in a. niger j 506 have higher activity and more production levels from page and zymogram of xylanase
尤其是在木聚糖酶譜帶檢測中發現,突變株發酵液中有三種類型的木聚糖酶,而出發菌株中只有兩種類型的木聚糖酶,並且通過考馬斯亮藍g250染色和瓊脂糖板上的透明圈發現,突變株中第三種類型的木聚糖酶不僅表達量很大,活力也很高。Effects of diverse environmental factors on the growth rate ( od4oo ) and nitrogenase activity ( ara ) of the strain w12 hi nitrogen - free culture were investigated in our experiments. the results implied that the strain w12 could easily adapt to different cultural conditions : it could use various carbon sources ( especially glucose, sucrose, malic acid, mannitol ), propagate quickly and fix nitrogen at a temperature range of 15 ? to 40 ? and at 25 - 35 ? for optimum, at a ph range of 4 to 8. 5, at a saline concentration range of 0. 01 % to 1. 5 % ; low nlv " concentration had little effect on its nitrogenase activity. ara could also be detected when it grow in the culture media with 5mmol / l ntv "
W12菌株對環境因子的適應性研究:無氮培養條件下,測定溫度、碳源、酸堿度、滲透壓對w12生長及固氮能力的影響,結果表明,在15 - 40下均能生長並表達固氮酶活性,其最適生長及固氮的溫度為25 - 35 ;能利用葡萄糖、蔗糖、蘋果酸、甘露醇等多種碳源生長並固氮,當培養基中同時存在蔗糖和蘋果酸時,細菌生長和固氮活性最強;在偏酸和偏堿的條件下( ph4 . 5 - 8 . 5 )均能保持較強的生長勢和較高的固氮酶活性,並能通過調節自身代謝平衡並適應環境的酸、堿性變化,使培養液趨于中性:能耐受較高的滲透壓,培養液中卜、 5 naci濃度對其生長和固氮酶活性影響不大,當naci濃度升至2時,菌株的生長勢及固氮酶活性才有所下降:低濃度的鉸對其固氮酶活性影響不大,在0Through screening a lot of mutants with the method of transparent zones and culture filtrate, the best four were obtained with high - yield of stable phb depolymerase, named as 02, 04, 09 and 14
以青黴( penicillium . sp ) ds9701為出發菌株,通過紫外線誘變分生孢子,採用透明圈初篩和搖瓶培養復篩的方法,獲得4個能穩定遺傳的phb解聚酶高產菌株。Secondly, the hyaluronate lyase gene ( hyl ) was cloned into the vector puc19 by pcr using the total ona sample ofs. equi as template and partially sequenced, too
Equi的總dna為模板,通過pcr方法,克隆了透明質酸分解酶基因( hyl ) ,測序后連接到表達載體pse380的trc啟動子下游,構建表達質粒pse380 - hyl 。The reproductive organ blister measles therefore recur, is because blister measles virus deep hiding in ganglion " the establishment gram kj medicinal preparation " series medicineis one kind of structure medicine, it ordinary disease - resistant poisonous medicine composition member is younger than several hundred times, can seep the nerve and the ganglion from the extroversion which suffers injury, is same along with it to sponge absoring water, layer upon layer strips the adsorption in the ganglion the crazy duplication viral body, the destruction virus s nucleotide duplication enzyme, causes it to be separated from the nerve is separated from the virus can massive gathering in the reproductive organ hypodermic, by now again coordinated the establishment gram venereal diseases kj medicinal preparation formidable anti - virus function, comprehensively struck kills the virus, caused the virus not to hide the place, thus achieved thoroughly permanently cured goal
安立克kj劑"系列藥物是一種微分子結構的藥物,它比普通抗病毒藥物的組成分子小幾百倍,能夠從外向內滲透進受損的神經和神經節,隨之就向海綿吸水一樣,層層剝離吸附在神經節里瘋狂復制的病毒體,破壞病毒的核苷酸復制酶,使其脫離神經.脫離出來的病毒會大量的聚集在生殖器皮下,這時再配合安立克性病kj劑強大的抗病毒作用,全面擊殺病毒,使病毒無藏身之地,從而達到徹底根治的目的Then, a piece of degenerate primer was designed according to the conserved amino acids of glycine betaine abc transporter system glycine betaine - binding protein as a reversed primer. combined with the opuaa - up, a 2. 3 kb fragment was obtained through pcr. blast result showed a fragment which contained the partial opuaa, the whole opuab and partial opuac sequences were obtained
再次,根據甘氨酸甜菜堿atp轉運系統底物結合蛋白的氨基酸保守序列設計下游簡並引物,與atp結合蛋白的上游簡並引物組合,經pcr擴增獲得2 . 1kb的條帶,測序后通過blast比較,結果顯示獲得atp結合蛋白基因的部分序列、通透酶的全部編碼序列和部分甘氨酸甜菜堿結合蛋白基因的核苷酸序列。Under the stimulation of inactivated vibrio anguillarum, the number of all hemocytes, the number of semigranular cells, the amount of po synthesized and po activity increased about 111. 3 %, 102. 9 %, 40. 4 % and 46. 3 %, respectively. the highest percentege of semigranular cells in all kinds of hemocytes after stimulation implies that it plays a key role in th e innate immune system of penaeus chinensis. morever, under the stimulation of polysaccharides and vibrios, the amount of po increases greatly, but the unit activity of po remains almost unchanged
本文利用脂多糖( lps ) 、 p一葡聚糖( p一1 , 3一glucan ) 、滅活哈維氏弧菌和滅活鰻弧菌4種免疫促進劑對中國對蝦進行了免疫刺激,通過分離純化、光鏡和透射電鏡觀察等技術對刺激前後中國對蝦酚氧化酶的產量、活性以及血細胞的數量、超微結構的變化進行了研究。A method for preparation of antithrombin ( at - ) concentrated from human plasma ' s fraction has been described. after preliminary treatment of plasma with the cold ethanol, the isolation of at - iii from the precipitate was performed by affinity chromatography on heparin - sepharose cl - 6b ; desalted by dialysis and concentrated
從人體血漿中提取較高純度的抗凝血酶( at - )的,通過低溫乙醇處理血漿,得到的沉澱經肝素-瓊脂糖兩次親和層析,並透析、濃縮獲得at -的初品。The basic function of the na, k - atpase is to maintain the high na and k gradients across the plasma membrane of animal cells. it has an important role in regulating the cell volumn, cytoplasmic ph and ca2 + levels through the na + / h + and na / ca exchangers, respectively, and in driving a variety of secondary transport processes such as na + - dependent glucose and amide acid transport
鈉鉀atp酶是維持生物體功能的最基本的酶之一,它通過形成細胞內外鈉鉀離子的濃度遞度來維持細胞膜靜息電位、滲透壓的穩定、營養物質的主動運輸與吸收等多種生理功能。Results the level of - ep, mda, mpo and pulmonary microvascular permeability were significantly higher in the group of iir than sham - operation group
分別測各組大鼠血漿-內啡肽及肺組織丙二醛含量、髓過氧化酶活性和肺毛細血管通透性。To confirm the approval of recombinant pcdnas - tgfjtf, gene and the results of its transfection, we used immuhistochemical staining ( sabc ). the department of or a logy 4 multiplication and differentiation of bmscs were observed by flow - cytometry ( tcm ), transilluminating electron microscope ( tem ) and other methods. the bmscs in controlled group was transfected with pcdna3 only
採用電泳檢測pcdna3 - tgf _ 1構建是否成功;通過tgf _ 1免疫組化染色檢測轉染是否成功;運用形態學觀察、透射電鏡( tem ) 、流式細胞儀( fcm ) 、堿性磷酸酶( alp )染色、型膠原免疫組化染色等方法,觀察轉染后bmscs增殖與分化情況。The effect of some factors, including the appropriate materials for isolating protoplasts, the concentrations of enzyme, period of digestion and temperatures, and osmotic pressure stabilizers on the isolation and regeneration of protoplasts in penicillium digitatum were studied. the results demonstrated that the purified protoplasts could regenerate through double layers of czapek medium containing 0. 7mol / l nacl. the regeneration rate could reach 24. 9 %
通過對制備材料、酶液濃度、酶解時間、酶解溫度、滲透壓穩定劑的種類和濃度等因素的實驗研究,得到了一套制備指狀青黴( penicilliumdigitatum )原生質體的有效方法,並在雙層培養基上初步實現了原生質體的再生,再生率可達24 . 9 。分享友人