連基因 的英文怎麼說
中文拼音 [liánjīyīn]
連基因
英文
x-linked gene-
Genes located on these chromosomes do not assort independently of sex and are said to be sex-linked.
這些染色體上的基因不能離開性別而自由組合，稱為性連鎖。Each of these color layers has its own genetic code which is determined by series of genes which combined eventually determine the color of the betta
每一顏色層都有自己的基因碼，這些基因碼受一連串的基因影響，最後決定了斗魚的顏色。The results also revealed that it was a reasonable choice to use carbenicillin as the antibiotics of inhibiting growth of remnant agrobacterium after co - culture, and when hygromycin was used as the selective agent, continuous selection at low concentrations ( 50 ~ 150mg / l ) produced the highest numbers of transgenic plants without escapes
試驗表明，在農桿菌介導高羊茅遺傳轉化中，抑菌劑宜選用梭節青霉素;以潮黴素作為選擇劑時，採用低濃度（ 50一15om留l ）連續篩選的方式比較合適，在該方式下，獲得的轉基因植株較多。Genes are linked together in cell nuclei on structurescalled chromosomes
基因位於細胞核中，它們互相連接形成的結構稱為染色體。Cercariae were collected, cultured in vitro and transformed to schistosomula in the rpmi 1640 medium with rabbit serum. the schistosomula were cultured in conditional media up to 96 hours. the number of schistosomula was counted and the death rate was calculated
將條件培養基與童蟲共培養， 96h內連續觀察計數，計算童蟲死亡率，與陰性對照比較，童蟲死亡率最高的基因池進入下一輪篩選。In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli
根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。The fusion protein was bactericidal active against staphylococcus aureus. in present study, we will truncate the none channel - forming do main, then attach the agrd to the pore - forming region ( k544 - i626 ) to construct a new engineered multidqmain protein machine - compact engineered peptide targeting staphylococcus aureus. such engineered peptide was constructed by linking the gene of staphylococcal agrd pheromone with the gene of c - terminal ( 1626 ) of colicin la pore - forming region ( k544 - i626 ) with site - directed mutation
利用點突變方法將金黃色葡萄球菌信息素agrd （ i型， ystcdfim ）的基因引入到大腸菌素fa梭基端1626基因上，並將限制性內切酶sacl酶切位點基因分別引入到大腸菌素fa的p4和k544上，通過酶切、膠回收、連接獲得含大腸菌素ia水性孔道結構域和金黃色葡萄球菌信息素agrd基因的重組質粒。Continued interchange of genes will counteract the effects of selection.
連續的基因交換將抵消選擇的效果。Changes in p - catenin expression or localization were found in cancer cells and tumor tissues, it had been reported that p - catenin translocated from membrane at the normal condition into the cytosol or nucleus in cancer cells. recently, p - catenin has been referred to an oncogene
研究發現，癌變的細胞中-連環蛋白的分佈發生了異常，由胞膜轉移到胞漿和胞核，因此，已把-連環蛋白歸為一個新的癌基因。Linkage disequilibrium of serotonin transporter gene and mood disorders
羥色胺轉運體基因的連鎖不平衡This time, using cdna of zmcdc5 as template, we amplify a sequence by means of pcr technology. and then, using restrict endoenzyme and ligase, we conjunct the 0. 8kb length dna sequence in a expression vector, pet - 30a. after induction, expression and purification, we obtained a 35. 4kda truncated fusing zmcdc5 protein which contains 267aa ( 647 to 914aa in zmcdc5 ). with the purified protein, we got its antibody and testified the antibody by means of western blotting and dot blotting
本實驗是以zmcdc5的cdna為模板，使用pcr獲得基因片段，再通過酶切連接，將得到的0 . 8kb的基因片段構建於pet - 30a表達載體上，經過誘導表達和純化，獲得zmcdc5的融合蛋白，其中包括了zmcdc5925個氨基酸中647 914共267個氨基酸殘基Then, we investigated the epistasis of gas using walsh - schema transform, and further evaluated the epistasis order for the < wp = 5 > continuous function optimization problems
利用walsh模式變換對遺傳演算法基因關聯問題進行了分析，並對連續函數優化問題的基因關聯階數進行了估計。According to these problems, we adopt to the method of mending material, optimize to fermentation media and partly ferment condition. finally, we excogitate a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified. with the plasmid pbv220 - ifnr, pbv220 - hgfa, pbv220 - hgfb, pbv220 - hpk5 that expresses serve as the model, adopting the biostat - c15l of b. braun company, utilize the method of mending material to ferment, through optimization fermentation media and optimization partly ferment condition ( ventilate quantity, stir speed, mend material speed ), eventually establishment a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified
以我室構建並穩定表達的重組質粒pbv220 - - ifn 、 pbv220 - hgf 、 pbv220 - hgf 、 pbv220 - hpk5為模型，分別從不同的表達宿主菌中篩選出一種適合大規模生產的菌種bl21 （ de3 ） ，該工程菌株連續傳代100代表達質粒不丟失，表達量穩定；採用b . braun公司的biostat - c15l自控發酵罐，運用分批補料技術分別進行四種工程菌的高密度發酵，通過優化工程菌發酵的培養基配方及優化部分發酵條件（通氣量、攪拌速度、補料速度） ，最終建立一種適于目的基因高效表達的高密度發酵工藝模式。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into
經限制酶消化和dna序列分析，證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列，在iptg誘導下兩種嵌合分子都獲得了分泌表達，表達產物主要集中在細胞周質空間。Reclamation, purification and linkage of them respectively, then sequencing and analyses of according gene structure were made, results show that the complete sequence length of corresponding pcr product from brussel sprouts, kohlrabi and kale are 1665bp, 1650bp and 1650bp, containing the first two exons and introns and 22bp of the third exon
對各pcr產物分別進行回收、純化、載體連接和序列測定及基因結構分析等，結果表明，該片段在抱子甘藍、羽衣甘藍和球莖甘藍三種作物中的全長分別為1665bp 、 1650bp和1650bp ，包括相應基因的前兩個外顯子和內含子以及第三個外顯子的22bp序列。Cut off beta fragment from plasmid prok. ii with hindlll and ecor i as insert, and cut pa into linear plasmid as vector fragment. link the insert and vector fragment together with t4 ligase, and the new vector with gene beta and gus was constructed
用hind和ecor雙酶切prok質粒，獲得beta基因片段作為插入片段，用hind和ecor雙酶切a質粒作為載體片段，將插入片段與載體片段相連，即構建成含有beta和gus的雙基因載體。A group of related and closely linked structural genes, together with the operator gene that controls their expression are collectively called an operon.
一組相關的、緊密連鎖的結構基因加上控制其表現的操縱基因，統稱操縱子。Operon is a unit of bacterial gene expression and regulation, including structural genes and cis - acting control elements in dna recognized by regulator gene product ( s )
控制某一代謝途徑的相關基因，緊密連鎖地排列在一起，受同一操縱子控制。2. 5 ul of 10 x reaction buffer, 1. 5 ul 25mm mgcl2, 0. 3 ul lomm dntp, 0. 5 ul taqdna polymerase ( 5 u / ul ), and lul ( = 20ng ) of primer were used in per reaction. each reaction was overlaid with one drop of paraffin oil. the initial denaturation step was used at 94 for 1 min 45 sec ; then denatured at 94 for 30 sec, annealed at 37 1 min, extended at iv b 72 for 2 min and repeated the cycle 45 times, at last, extended at 72 ' c for lomin
等（ 1995 ）利用rapd標記區分美國東部一雜交地帶的蟋蟀的兩個姐妹種， allonemobiusfasciatus和a . socius ，並於1998年使用rapd和異型酶標ic做出了這兩種蟋蟀的基因連鎖圖；國內田英芳、鄭哲民（ 20of ）首次將rapd技術運用於蟋蟀總科的分子系統學研究中，採用2種引物對7種蟋蟀進行了基困組dna多態性研究，並應用upg問a法構建樹狀圖，椎測系統發生關系分享友人