連接酶的結構 的英文怎麼說

中文拼音 [liánjiēdejiēgòu]
連接酶的結構 英文
dna ligases: structuresdna
  • 連 : Ⅰ動詞1 （連接） link; join; connect 2 （連累） involve （in trouble）; implicate 3 [方言] （縫） ...
  • 接 : Ⅰ動詞1 （靠近;接觸） come into contact with; come close to 2 （連接; 使連接） connect; join; put ...
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 的 : 4次方是 The fourth power of 2 is direction
  • 結 : 結動詞（長出果實或種子） bear （fruit）; form （seed）
  • 構 : Ⅰ動詞1 （構造; 組合） construct; form; compose 2 （結成） fabricate; make up 3 （建造; 架屋） bui...
  • 連接 : connect; fit together; link; marry; mate; joint; association trail; linkage; concatenate; concate...
  • 結構 : 1 （各組成部分的搭配形式） structure; composition; construction; formation; constitution; fabric;...
  1. The fusion protein was bactericidal active against staphylococcus aureus. in present study, we will truncate the none channel - forming do main, then attach the agrd to the pore - forming region ( k544 - i626 ) to construct a new engineered multidqmain protein machine - compact engineered peptide targeting staphylococcus aureus. such engineered peptide was constructed by linking the gene of staphylococcal agrd pheromone with the gene of c - terminal ( 1626 ) of colicin la pore - forming region ( k544 - i626 ) with site - directed mutation

    利用點突變方法將金黃色葡萄球菌信息素agrd （ i型， ystcdfim ）的基因引入到大腸菌素fa梭基端1626基因上，並將限制性內切酶sacl酶切位點基因分別引入到大腸菌素fa的p4和k544上，通過酶切、膠回收、連接獲得含大腸菌素ia水性孔道結構域和金黃色葡萄球菌信息素agrd基因的重組質粒。
  2. Levels of fasting blood glucose and 24h urinary microcontent of albumin 24 h malb were determined dynamically ; the serum glycosyl hemoglobin hba1c was determined after the last medication ; the ultrastructural changes of kidney were observed by transmission electron microscope ; the expressions of collagen, fibronctin, laminin ln, and the ecm metabolism influencing factors, including mmp2, tissue inhibitor of metalloproteinase timp2, transfer growth factor 1 tgf 1 in renal tissue were detected by immunohistological chemistry and image collecting analytical system

    動態檢測各組大鼠空腹血糖fbg 24h尿微量白蛋白24h malb ，末次給藥后測定大鼠血漿糖化血紅蛋白hba1c透射電鏡觀察各組大鼠腎臟超微結構改變，應用免疫組化技術及圖像採集分析系統測定各組大鼠腎臟組織中型膠原c纖維連接蛋白fn層粘連蛋白ln的表達，測定影響ecm代謝的基質金屬蛋白酶2 mmp2基質金屬蛋白酶抑制劑2 timp2及轉化生長因子1 tgf 1的表達。
  3. The predicted vfcpkl protein with 493 amino acids contains all the structural characteristics of reported cdpks and there are four domains of a variable domain, a kinase domain, an autoinhibitory domain and a calmodulin - like domain from n - to c - terminal end

    由編碼區推測的vfcpk1的493個氨基酸序列具有已報道的cdpk的所有典型結構特徵，由n端到c端可分為可變區、激酶區、連接區和調節區四個結構域。
  4. To construct eukaryotic expression vector of mbl gene with codon 54 point mutation, the target sequence in pgem - mbld plasmid, which conains mbl cdna with codon 54 mutant allele, was amplified by pcr. after the cdna fragement and plasmids pci - neo were prepared by digestion with sma i and sal i, the fragment was inserted into sma i and sal i site in pci - neo eukaryotic expression vector, and the recombinant vector, named pci - mbl54, was obtained. the pci - mbl54, digested with restriction enzymes, was found to contain the point mutation mbl cdna by agarose gel electrophoresis analysis

    本實驗以ggc54gacmbl突變為研究對象，選用真核表達質粒pci - neo ，根據已構建好的含54位密碼子突變型mbl基因t載體的結構，設計合成新的引物， pcr擴增54位密碼突變型mbl基因，凝膠回收，雙酶切pcr產物和pci - neo質粒， t4連接酶連接，將前者克隆至後者的sma和sal位點，轉化大腸桿菌xl - 1blue ，氨芐選擇培養。
  5. In this research, the gpv hl isolate was propagated with 13 day ' s duck embryos. a pair of primers gflgr used to arnplify vp3 gene was designed using oligo4. i software according to the whole nucleotide sequence of gpv b isolate published by zadori. the major structural protein vp3 gene was amplified from the dna of gpv hl isoiate by polymerase chain reaction ( pcr ), and then cloned into pmdl8 - t vecter

    根據zadori等發表的gpvb株全基因核苷酸序列，藉助oligo4 . 1軟體設計了1對用以擴增主要結構蛋白vp3基因的引物gf / gr ，通過pcr技術，從病毒基因組dna中擴增出病毒主要結構蛋白vp3完整基因片段，經酶切鑒定后直接與pmd18 - t質粒載體連接。
  6. In this study, the recombinant plasmid pmd - 18t - pea - h3 was cleavaged with ncoi, xhoi and inserted into the expression vector pet - 28c and subsequently subjected to restriction endonuclease analysis and sequencing, the result indicated that the prokaryotic expression vector pet - 28c - pea - h3 was constructed successfully. after the expression plasmid was extracted and transformed into expression hosts bl21 ( de3 ) of e. coli, the transformed hosts were induced by iptg, bysds - page and elisa analysis of host protein. the expression of the objective gene was detected, and it could account for 16. 28 % of the total host protein. inclusion body was prepared from the incubating expression hosts induced by iptg

    同時將原核表達載體pet - 28c用nco ， xho雙酶切，回收酶切產物，將回收的酶切產物pea ， h3 ，載體進行連接，並轉入dh5感受態細胞內，培養12 - 18小時后，挑取陽性菌落，經nco ， xho雙酶切分析及pcr檢測，篩選到陽性克隆，其質粒測序結果表明成功地構建了毒性基因缺失的pea與人組蛋白h3融合基因的原核表達載體。
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