連通序列 的英文怎麼說
中文拼音 [liántōngxùliè]
連通序列
英文
connected sequence-
In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli
根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。Summary the front chapter, discuss the exercisable method of urban design, dissertate it from several aspects : space sequence, interface consecution and environment facilities
通過對以上章節的總結，探討線性休閑空間與動態景觀創造可操作性的城市設計手法。從線性休閑空間整體序列、界面連續、環境設施等幾個方面進行論述。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。Ghrelin regulates pituitary growth hormone ( gh ) secretion. in the study, we obtained the cdna sequences of preproghrelin and the cdna encoding the ghrelin of duck, goose and emu by using rt - pcr and 3 " - race methods. and then deduced the amino acid sequences of preproghrelin and ghrelin in duck. goose and emu. sense and antisense primers were designed on the basis of chicken ghrelin cdna sequence ( dest accession no. ab075215, 836bp ) and proventriculus cdna was performed as the template
Ab075215 ， 836bp ）設計引物，以鴨、鵝、鴯鶓腺胃cdna為模板，通過rt - pcr及3 - race的方法，將各個產物經過回收，再經連接轉化，克隆測序，拼接后得到鴨、鵝、鴯鶓preproghrelin基因的cdna序列及ghrelin的cdna序列，並由此推測出preproghrelin及ghrelin的氨基酸序列。The c - phycocyanin ( cpc ) operon of blue - green alga ( or cyanobacteria ) arthrospira platensis fachb341 was cloned, sequenced and characterized by using chromoseme walking method. the sequence includes cpcb gene ( 519 bp ), cpca gene ( 489 bp ), cpch gene ( 357 bp ), and upstream sequence of cpcb ( 427 bp ) and upstream sequence of cpch genes ( 184 bp ), 111 bp of phycocyanin intergenetic spacer ( pc - igs ). upstream sequence of cpcb gene was ligated into promoter - probe vector pegfp - 1 and transformed into three systems : e. coli, synechocystis pcc 6803 and a. platensis fachb341 by supersonic and electrophoresis methods
根據genbank中報道的節旋藻藻藍蛋白基因序列設計引物，首先克隆了鈍頂節旋藻（ arthrospiraplatensisfachb341 ）藻藍蛋白操縱子中亞基基因、亞基基因部分序列及二者之間的間隔區序列（ pc - igs ）並進行序列測定，然後根據此測序結果設計引物，通過染色體步移法克隆得到藻藍蛋白操縱子長度為2086bp的基因片段，其中包括藻藍蛋白亞基基因（ cpcb ， 519bp ） ，亞基基因（ cpca ， 489bp ） ，連接蛋白h基因（ cpch ， 357bp ） ，亞基基因上游啟動子序列（ 427bp ）以及各基因之間的間隔區（ pc - igs ， 111bp ； cpch與cpca間隔區， 184bp ） 。2. an up - dated method was employed to purify tumv in this research. using the protease k method, we acquired the viral genome - rna. a pair of specific primers was designed and synthesized based on the nucleotide sequences of tumv coat protein genes reported before and rt - pcr was used to clone the cp genes of the six tumv isolates
應用改進的蕪菁花葉病毒的提取方法從病葉中提取病毒粒體，應用蛋白酶k法從病毒粒體中提取病毒rna基因組，根據已報道的tumv的cp基因序列，設計併合成了一對特異引物，利用rt - pcr法克隆了6個分離物的外殼蛋白基因，與克隆載體puc19連接后通過熱激法轉化大腸桿菌dh5 。Connected sequence of functors
函子的連通序列The framework of topology is based on apriori with the idea of " isomeromorphism ", using the techniques of graph sequential expression and label - connectivity determination. topology can analyze the complex relations among the objects in th
這是一個以apriori思想為主體，以先同分后異構為框架，以圖的序列化及矩陣表示和標號連通判定等技術為手段的一個綜合演算法。Chapter two study iteration of a serial of polynomial, discussed the sufficient and necessary conditions and denseness of the julia set, the relative random dynamical system is constructed by some high degree polynomial. in addition, it discuss the mandelbrot set of a kind of polynomial
本文的第二章主要研究多個函數的特定迭代序列，討論了高次多項式的隨機復動力系統的julia集的連通的充分必要條件以及稠密性問題，同時還討論了一類多項式函數的mandelbrot集。We can obtain the sequence of speckle interferograms by continuously recording the temporal speckle interference field caused by the object temporal deforming using the imaging system
通過攝像系統連續地採集這一時變散斑場，可獲得一系列時間序列散斑干涉圖。Benzyl chloride were used for extracting genomic dna of aspergillus. niger 14, about 1. 5kb specific fragment was obtained from genomic dna of aspergillus. niger 14 by pcr amplification with primers ( forward primer5 " ataggcatcatgggcgtctct3 " reverse - primer5 " cagctaagcaaaacactccgc 3 designed according to the known sequences of the phytase gene in the gene bank and pyrobest ? dna polymerase, after ligated with pmd18 - tvector, transformated into e. colidh5a competent cell successfully. 3. nucleotide sequence analysis of the cloned fragment revealed the presence of the whole phya gene in pcr product
用氯化芐法提取了aspergillus . niger14 ~ #基因組dna ，根據genebank中已知的黑麴黴植酸酶基因序列設計出一對特異性引物（上游引物： 5 ataggcatcatgggcgtctc3下游引物： 5 cagctaagcaaaacactccgc3 ） ，採用pyrobest ~ （ tm ） dnapolymerase （高保真dna聚合酶） ，通過pcr方法從aspergillus . niger14 ~ #基因組dna中擴增出了預期的1 . 5kb左右的特異性產物，將其與pmd18 - tvector連接后，轉化e . colidh5菌株的感受態細胞，經質粒抽提、酶切鑒定，確認該目的產物已得到成功克隆。The main contributions of this dissertation are as follows : 1 ) forms a scheme of network maintance as well as realiztion of the problem testing software after a thorough analysis of the regular network problems and a pertinent comment on the merits and demerits of the network detection and maintainance methodology. 2 ) presents approaches to the realization of principles of network health testing, network resources list and connectivity testing in the network problem testing software, and gives a in - depth analysis of the key issues in design process with vivid flow charts and convincing testing results. 3 ) ponits out possible problems and offers feasible solutions in the migration and development of the embedded software from six aspects, touching upon byte order, semaphore, coding optimization, etc. 4 ) discusses the exception handling mechanisms and debugging process for the sake of improving software robustness
本文的主要工作如下: 1 ）研究了以太網常見故障並對網路探測維護方式的優缺點進行了詳盡分析，根據項目要求確定了本系統的網路維護方案，並對故障測試軟體的總體實現方案進行了設計; 2 ）闡明了網路故障測試軟體中網路健康測試、網路資源列表、連通性測試的原理，提出了相應的實現方案，分析了設計過程中的關鍵問題，並給出軟體流程圖和測試結果; 3 ）從位元組序、信號量、代碼優化等六個方面談論了嵌入式軟體在移植開發中的注意事項; 4 ）為提高軟體的健壯性，討論了本嵌入式系統軟體採用的異常處理機制以及軟體調試過程中的。On the basis of displacement - time series of the slope, a nonlinear dynamic model is set up according to backus generalized linear inversion theory in this paper. due to the equivalence beween autonomous gradient system and catastrophe model, a standard cusp catastrophe model can be obtained through variable substitution. the method is used in analysis of displacement data of huangci landslide and wolongsi landslide and in understanding how slopes evolve before sliding. the result shows that the nonlinear dynamic model can make satisfactory prediction result. is it most important that there is a sudden fall of d, which indicates the occurrence of catastrophe ( when d = 0 )
研究表明，滑坡變形失穩過程具有混沌和分維特性，可以用分形理論來研究滑坡預測問題，基於對任一連續函數，至少在較小的鄰域內可以用多項式任意逼近的數學理論，運用改進的backus廣義線性反演理論，以斜坡位移時間序列為基礎，反演了斜坡演化的非線性動力學模型。並利用自治梯度系統與突變模型的等價性，通過變量代換得到標準的尖點突變模型。On the basis of sequence stratigraphy analysis of bayindulan sag, wuliyasitai depression, jiergalangtu sag and saihantala sag, the integrated sequence stratigraphy section of lower cretaceous has been established and the vertical sequence of sandbodies distribution in three - order sequence has been concluded
本文通過對巴音都蘭、烏里雅斯太、吉爾嘎朗圖和賽漢塔拉等凹陷層序地層分析，建立了二連盆地下白堊統層序地層綜合剖面，並總結了三級層序框架內砂體分佈的垂向序列。In chapter 5, the complex envelop simulation block diagrams of fh transmitter and receiver are presented at first. then key techniques of simulation system are discussed, including frame processing structure, fh sequence generator, etc. finally, simulation models of fh transmitter, receiver and jammer are presented. the influence of frequency excursion on performance of multi - tone continuous wave jamming is analyzed
第五章首先設計了跳頻發信機成員和接收機成員的復包絡模擬框圖;其次討論了跳頻模擬系統實現的關鍵技術，包括幀處理結構、跳頻序列發生器等;最後給出了跳頻通信發信機、接收機以及干擾機成員的模擬模型，分析了頻率偏移對多頻連續波干擾性能的影響。Because of its fully sequenced genome and the most complete, contiguous nucleotide sequence of centromeric dna in plants, arabidopsis was chose as the base to construct plant artificial chromosome. we identified two candidate yac clones, cic8h8 and cic6f6, which are both proved to have the 178 bp arabidopsis centromeric repeat sequences
我們通過pcr和序列分析，選擇了含有擬南芥著絲粒區178bp串連重復序列的yac克隆作為構建人工染色體的基礎，同時也克隆了擬南芥的端粒和ars等序列。Methods : a set of oligonucleotide primers were designed and used to amplify the vh and vl gene from anti - hbsag fab antibodies screened from phage antibody library. the products were cloned into puc19 vector and their sequences were analysed. the vh and vl gene fragments were tethered by a peptide linker and a leader sequence coding region, with the leader sequence added at 5 " terminus of each gene ( l - vh - linker - vl ) and designated as l - scfv
方法以從噬菌體抗體庫中篩選獲得的抗hbsag的fab抗體基因為模板，分別擴增出其輕、重鏈可變區（ v _ l 、 v _ h ）基因，通過重組pcr方法將輕、重鏈可變區基因用連接肽（ gly _ 4ser ） _ 3的編碼序列連接，並引入前導肽編碼序列，構建具有l - v _ h - linker - v _ l結構的單鏈抗體基因。Fuzzy connectedness based segmentation of color image slice sequence
基於模糊連通性的彩色圖像切片序列分割方法In this article, the misgurin gene and adaptor are synthesized according to the amino acid sequence reported in the genebank and the need of construction and expression. adopted a new strategy, multiple copy gene is ligated in the same direction. and then it is cloned into expression vector plasmid ppic9k
本文根據genebank登錄的氨基酸序列，同時考慮構建和表達的需要，化學合成了misgurin基因和接頭，採用一種新的策略，在體外將基因多拷貝同向串連，並將其克隆于表達質粒ppic9k中，通過鑒定並測序正確后，電轉化真核表達宿主? ?畢赤酵母菌株gs115 ，通過營養缺陷型培養基篩選重組子，再利用g418抗性篩選出整合有多拷貝外源基因的重組子。The order of our discussions " about these tasks is as follows : firstly, we pay more attention to the characteristics and difficulties of its environment including the concept, typical system model, main challenges, mobile network connection and soft application. secondly, according to mobile specialties of the environment we make the sort of data into four kinds : general data, time series, spatial data and time - spatial data, and present general processing of data mining. lastly, we discuss the methods of data mining of these four kinds respectively : after the introduction of the actuality of data mining of every kind, an algorithm of rule updating based on rough set is given, then put forward the processing of data related to mobile users and flow chat according to characteristics of the other three kinds
本文對以上任務的討論順序安排如下：首先是對移動計算環境的技術特點和難點進行討論，包括移動計算的概念和典型系統模型、主要挑戰、移動聯網以及軟體應用這幾個大的方面；其次根據移動環境的移動特性把移動計算環境中的數據分為普通數據，時間數據，空間數據以及時空數據，提出了在移動計算環境中數據挖掘的一般流程；接下來分別對這四類數據進行挖掘演算法的討論：每一部分都是先介紹該類數據的挖掘方法研究現狀，對于普通數據，針對我們已提出的一種挖掘演算法-粗糙集演算法（ rs ） ，提出了對應的規則更新演算法，對於後三種數據，本人根據其在移動計算環境中的特點分別提出了與移動用戶相關的該類數據的一種具體的處理方法和演算法流程圖，包括基於移位連接方法的多屬性時間序列的挖掘演算法，基於apriori演算法的空間關聯規則數據挖掘方法以及關于移動用戶移動模式的時空數據挖掘方法，並用matlab對其中的規則更新演算法和時間序列的挖掘演算法這兩方面進行了實例模擬。分享友人