達重 的英文怎麼說
中文拼音 [dázhòng]
達重
英文
tatsuju-
Conclusions : prokaryotic and eukaryocytic expression plasmids of the shortened hepatitis b surface antigen were successfully constracted, and the target proteins expressed by iptg induced in escherichia coli. as well as in eukaryocyte ( hepg2 and cos - 7 ), then their antigenity were detected
結論:截短的乙型肝炎表面抗原分子的原核和真核表達』重組質粒成功被構建及分別在人腸桿菌efl得到誘導表達和存貞核細胞ifj表達,並檢測劍其表達產物的抗原特性。Expression of b lymphocyte stimulator from pet plasmid using lactose as inducer
載體表達重組蛋白的研究We obtained the full length gene of hbfgf coding sequence with pcr, and adjusted the g + c content according the software dnasisv2. 5, and replaced the cys78 and cys96 with serines by site - directed mutagenesis. 2. sequence result suggested one of the recombinant is correctly synthezied and cloned
用pcr方法合成了hbfgf編碼區全長,其中前20個氨基酸的g + c含量按暨南大學碩士學位論文:大腸桿菌表達重組hbfgf結構和功能優化摘要照計算機軟體計算的結果進行了調整,第78和96位上的半瞇氨酸被突變為絲氨酸。The plasmid plncxhlf which is combining with 2366bp human lactoferritin ( hlf ) cdna complete sequence and 800bp regulating sequence upsteam by the - lactoglobulin gene promoter is prepared by normal steps. then it is used in the following experiments. the recombinant plasmids plncxhlf were introduced into mouse mammary tumor cell line ma - 782 by liposome entrapment
本研究將含有人乳鐵蛋白( hlf )基因2366bpcdna序列完整序列和800bp的奶山羊-乳球蛋白基因5 』端調控序列的乳腺特異表達重組載體plncxhlf按常規方法提取、純化、 pcr 、酶切鑒定后,用於細胞表達和精子介導轉基因動物的研究。Port from taicang port, go up stream along yangtze river directly to chongqing
碼頭從太倉港沿長江黃金水道溯流而上,可直達重慶。Razor scooters. - return to wolfenstein
-速可達-重返虎穴We are going to arrive at chongqing jiangbei airport 15 minutes behind schedule because of strong headwinds
因遇上強烈頂風,我們到達重慶江北機場將晚點十五分鐘。Objective : to construct prokaryotic and eukaryocytic expression plasmids of the shortened hepatitis b surface antigen, and express the target proteins by iptg induced in escherichia coli
目的:構建截短的乙型肝炎表面抗原分子的原核和真核表達重組質粒,然後分別在大腸桿菌中誘導表達並純化表達蛋白及在真核細胞中表達目的基因,並檢測其抗原特性。Conveying important information by means other than sound
用非聲音方法傳達重要信息The first fule of leadership is to save yourself for big decision. don ' t allow your mind to become cluttered with the trivia. don ' t let yourself become the issue
領導的第一個法則是讓自己能夠下達重大的決定。因此不可為瑣碎的事物分神,也不能使自己成為爭辯的問題之一。Once the recombinant virus has been i - dentified, we amplified the p - 1 stock to attain the large scale, high - titer viral stock in order to initiate expression studies. the recombinant angiostatin was produced from spodoptera frugiperda 9 ( sf9 ) insect cells infected by the high titer virus stock we have prepared. the time course for expression of recombinant protein was detected by sds - page and western blot, which can determine the optimal multiplicity of infection ( moi ) and the appropriate time of harvest for the protein
表達重組蛋白angiostatin :用制備好的帶有矗s標記的融合性angiostatin基因的高滴度重組桿狀病毒貯存液感染草地貪夜蛾sfg細胞,用sds page電泳和hsternblot對感染不同時間後分泌的重組蛋白做時間表達分析,依此確定最適的感染復數( moi )和感染時間,以達到重組蛋白表達水平最適化,而後大規模進行重組蛋白的表達, sds page用來分析重組蛋白, westernblot用來在蛋白表達水平低的情況下檢測表達的特異重組蛋白。Construction of recombinant pichia pastoris containing cdna encoding mature peptide of human bone morphgenetic protein - 7 bmp
7成熟肽畢赤酵母表達重組子的構建High - density expression of recombinant porcine insulin precursor by pichia pastoris
畢赤酵母高密度表達重組豬胰島素前體的研究An expression vector with fragment 9 in antisense orientation was constructed to block the expression of the relevant gene ( fragment 9 related gene, fnr gene ) in vero cells. interestingly, we found that the nontargeted mutation frequency induced by mnng was increased significantly, implicating that the product of the blocked gene may be involved in the inhibition of nontargeted mutation
利用反義核酸技術構建含反向插入9號片段的真核細胞表達重組體並轉染細胞,以獲得反義rna阻斷vero細胞中相應基因的表達,發現mn 』 ng誘發的非定標性突變頻率顯著增高,提示被阻斷的相關基因的表達產物可能參與抑制非定標性突變的發生。Expressions of fas fasl in colorectal carcinoma and adjacent mucosal tissues
1基因真核表達重組質粒的構建及免疫效果Four of the six resulting recombinants contained two genes arog and qutb, the other contained three genes arog, qutb and arob. the catalytic activities of both ds and qdhase increased diffierently, but enzyme activities of the recombinant harboring pbv - prpl - qutb - pl - arog were best
六種重組子都實現了目的蛋白的表達,表達產物ds和qdhase酶活性有不同程度的提高,其中pbv - prpl - qutb - pl - arog串聯表達重組子表達產物二種酶活性ds和odhase都比較高,是優化的重組子。Chloe : sending redeployment orders now
正在下達重新部署的命令I have training, optimistic heavy feeling rich love healthily well
我端正健康有修養,豁達重情富愛心。Expression vectors of whole il - 6r gene and its carboxyl terminal partial gene sequence were constructed by pcr and gene recombination technique, respectively
6r基因全長和矮基端部分編碼序列的表達載體,並導入大腸桿菌dhsq中,通過iptg誘導表達重組融合蛋白。With the development of dna recombination technology, there are also some reports about the expression of recombined ifn protein by prokaryotic expression system, but the expressed proteins often consist in the form of inclusion body = the operations about extracting and purifying proteins are very complicated, biological activity of proteins are not high
隨著dna重組技術的發展,國內外亦有利用原核表達系統表達重組ifn蛋白的報道,但表達的目的蛋白常以包涵體形式存在,提取純化操作步驟復雜、蛋白活性不高。分享友人