酶抗體法 的英文怎麼說
中文拼音 [kàngtǐfǎ]
酶抗體法
英文
enzyme antibody method-
In the experiment, the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked. it was expressed in e. coli and its protein was determined. after having been properly modified, the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing, which is the prerequisite work for genetic transformation
本實驗從抗除草劑轉基因bobwhite小麥中，利用pcr克隆的方法擴增出bar基因全長，並在原核表達系統中表達，鑒定表達蛋白的活性，將能夠正確編碼ppt乙酰轉移酶的bar基因片段，經過適當的修飾構建入真核表達載體。Detection of antibodies against caprine arthritis - encephalitis virus - protocol of enzyme - linked immunosorbent assay
山羊關節炎-腦炎抗體檢測方法.酶聯免疫吸附試驗However, amphioxus ubf80 also showed some unique characters such as its amino acid residues at sites 39, 54, 63 and 74 in the cep. it appears that amphioxus not only represents an organism tr ansitional from invertebrates to vertebrates in phylogeny but also a possible diverged form radiated from the chordate ancestor at early chordate evolution. the possibility that some mutations in ub genes have taken place in different lines early at the divergence of plants and animals is also discussed
發現文昌魚a二phi屍rxv基因是具有線粒體和過氧化物酶體信號特徵（ mitochondrialandperoxisomalsortingsignals ）的抗氧化物酶基因;通過序列比對，我們把屍rxv歸為l一cys和2一cys之外的3一cys類，從而把屍rx基因家族的兩種分類方法統一了起來。The main study in this paper included as follows : the content and distribution of heavy metals in sediments and benthic organisms from the sewage stream in guangzhou city ; the acute toxicity and joint toxicity of mercury and selenium to swordtail fish ( xiphophorus helleri ) ; the damage of mercury to the indexes of antioxidant system in the gills and livers in swordtail ( including the measurement of the activities of total antioxidative capacity [ t ~ aoc ], superoxide dismutase [ sod ], glutathione peroxidase [ gsh - px ] and the concentration of malondiald - ehyde [ mda ] ) and the relief effects of selenium on it, as well as the physiological damage of mercury on the tissues, namely : the antagonistic effect of na + - k + ~ atpase activity on the tissues between selenite and mercury, and the ultrastructural damage under the exposure of mercury
研究內容主要有：廣州市河涌沉積物及底棲生物體內重金屬含量及評價；汞和硒對劍尾魚的急性毒性和聯合毒性及安全濃度的評價；汞對劍尾魚鰓和肝臟中抗氧化系統的毒性，包括對總抗氧化能力、超氧化物歧化酶、谷胱甘肽過氧化物酶活力及丙二醛含量的測定及硒對其保護作用；汞對劍尾魚組織生理毒性即：汞對na ~ + - k ~ + atpase活力的影響及硒的保護作用和汞和對劍尾魚組織超微結構的損傷等。以高氯酸?硝酸消化法和火焰原子吸收分光光度法測定了廣州市河涌沉積物和底棲生物中重金屬含量。Correlation between signal cutoff ratios of anti - hcv enzyme immunoassay and their true positivity in blood donors
獻血員丙型肝炎病毒抗體酶免疫法檢測試劑的測量值與其真陽性的相關性This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected
本研究以蛋白質分子設計的理論和方法研究避孕疫苗，將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合，獲得新抗原- 35肽序列；並在合成、純化後分別與弗氏佐劑、免疫刺激復合物（ iscoms ）混合后免疫不同遺傳背景的雌性小鼠，觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變：以及在ivf下，新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。The determination of human thymidine kinase ( htk ) in human serum, which is a key indicator of cancers can give information for the diagnosis and treatment of the malign diseases. the protein a layer was first self - assembled onto the gold electrode surfaces of quartz crystals, the monoclonal antibodies were then orientedly immobilized through the specific binding between the fc terminals of the antibodies and the self - assembled protein a. with this sensor, the affinity constant of antigen - antibody binding was estimated to be 1. 85 106 l / mol according to the scatchard ’ s plotting method, which proved the high bioactivity of antibody. finally, an amplified piezoelectric immunosensor was designed to determine the htk in
實驗中將蛋白a吸附於鍍金壓電石英晶體電極表面，用於定向固定htk單克隆抗體，成功研製了檢測htk的壓電石英晶體傳感器，並基於標準scatchard繪圖法，計算出免疫反應的親和常數為1 . 85 106l / mol ，證明該單克隆抗體具有較高的免疫活性;同時基於酶催化沉澱技術，設計了的檢測htk的質量放大壓電石英晶體傳感器，該傳感器可在0 . 1 - 10ng范圍內對htk進行定量檢測，應用此傳感器成功地對5種癌癥病人血清中htk的濃度進行了測定，實驗結果為癌癥的臨床診斷與治療提供了參考。In this paper, the relationship between cyanide - resistant respiration and expression of alternative oxidase ( aox ) using polyantibodies prepared by synthetic polypeptide in mung bean seedling, as well as the effects of vernalization on cyanide - resistant respiration and expression of aox in winter wheat were studied respectively. in the first part, twelve peptides, including eight conservative amino acid residues in the amino acid sequence of hydrophilic s helix of aox. were synthesized by solid - phase method
第一部分，我們按照交替氧化酶（ alternativeoxidase ， aox ）位於線粒體內膜外側親水區s螺旋的氨基酸序列，用固相法合成由12個氨基酸殘基組成的多肽，將此多肽與-牛胰凝乳蛋白酶原a相連，用作半抗原，免疫家兔制備人工合成12肽的抗體。Study on elisa method to detect the antibody of aev
酶聯免疫吸附試驗檢測禽腦脊髓炎抗體方法的研究In this paper, wheat cultivars lovrin 10 ( resistant ) and 5389 ( susceptible ) were selected as materials in this system. the mesophyll protoplasts of wheats ( mpw ) were isolated using cellulase and pectolase digestion. by the indirect immune fluorescent labeling, microtubules ( mts ) pattern in mpw were showed clearly under the confocal laser scanning microscope ( clsm )
本試驗以抗（洛夫林10 ） 、感（ 5389 ）不同的兩小麥（ triticumaestivuml . ）品種為材料，採用酶解法制備小麥葉肉原生質體，利用間接免疫熒光方法結合激光共聚焦掃描顯微技術對小麥葉肉細胞原生質體的微管骨架進行了清晰的標記，並探索了微管骨架標記的影響因素。In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company
實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。Research interests : organic synthesis, including synthetic methodology, divergent oriented organic synthesis, asymmetric synthesis, asymmetric synthesis of small molecules with biological significance ( alkaloids, amino acids, pheromones, nucleosides analogues, enzyme inhibitors, receptor agonists and antagonists, bioactive heterocycles etc ) and chiral drugs
研究興趣：主要從事有機合成化學及相關的化學生物學研究，包括合成方法學、多樣性導向有機合成、不對稱合成、有機小分子調節劑（生物堿，氨基酸，昆蟲信息素，酶抑制劑，受體拮抗劑，核苷類似物等）和手性藥物的不對稱合成。Calli were induced from internode segments of the methionine resistant plantlets of astragalus melilotoides. the highest yield of protoplasts ( 2. 1 x 106 / g f. wt. ) was obtained from 8 - day - old friable calli after subcultured on fresh medium
用草木樨狀黃芪甲硫氨酸抗性變異系植株莖切段誘導的松軟愈傷組織為材料，通過酶法分離出大量有活力的原生質體。Protocol of detection antibodies against avian viral arthritis virus - enzyme - linked immunosobert assay
雞病毒性關節炎抗體檢測方法.酶聯免疫吸附試驗Abstract : the determination of aminoglycoside antibiotics in the biological fluid has been reviewed. the methods mainly included bioassay, thin - layer chromatography, high - performance liquid chromatography ( hplc ) and a variety of immunoassay ( ia ), for example, enzyme immunoassay, fluorescence polarization immunoassay, quenching fluorescence immunoassay, chemiluminescent immunoassay ， etc., in which hplc and ia were used more often
文摘:綜述了氨基糖苷類抗生素的體內分析方法.主要有：生物學分析法；薄層色譜法；高效液相色譜法；差示脈沖極譜及各種免疫法，如酶免疫法，熒光偏振免疫法，膠乳凝集抑制速率比濁法等.其中高效液相色譜法和免疫法應用較多A dot - enzyme - linked immnosorbent assay ( dot - elisa ) for the detection of antidodies against newcastle diesease virus ( ndv ) was established by using purified ndv and self - made enzyme - labeled anti - chicken igg, the mehod was also evaluated through it ' s application
本研究用提純的新城疫病毒和自製的酶標抗體建立了檢測雞新城疫抗體的dot - elisa方法。試驗中用f _ （ 48 ） e _ 9株和lasota株提純抗原並對兩者進行了比較。Preparation of monoclonal antibodies against troponin i and development of one - step sandwich elisa
單克隆抗體制備及酶免疫法的建立Methods : sandwich elisa assay was used, w6 / 32 mcab serving as solid phase antibody and 3 2m antibody as the first antibody. the second antibody - hrp conjugate was added for coloration. standard curve was obtained by shla - i standard reagent in serial dilution. the amount of shla - i in the samples was determined : 1
方法：以w6 32包被酶標板，捕捉樣品中可溶性hla ，加入一抗2m抗體，再加酶標二抗及底物顯色。根據可溶性hla -的不同濃度標準品顯色后的od值繪制標準曲線： 1To develop a rapid diagnostic test based on serum antibody to sars virus. coupled with clinical observations, the antibody test and the rt - pcr test could help in making rapid diagnosis, and thus allowing effective treatment using ribavirin and steroids at an early stage
發展出一套sars抗體的快速血清測試方法，並配合臨床觀察，此抗體測試及逆轉錄-多聚酶測試（ rt - pcr ）能作出快速而準確的診斷，有助於發病初期使用利巴韋林及類固醇進行有效的治療；A eukaryotic expression vector pcdna3. 1 - cptl was constructed by insert cp77 gene into the vector pcdna3. 1 which is used in nucleic acid immunization. the vector was immuned the balb / c mice by the method intramuscular injection after extracted and purified in great deal. immu - nological reaction was induced by the expression of cptl after the vector entered into the mice body
本研究通過限制酶將cpti基因片段從載體pbluel3上切下，插入真核表達載體pcdna3 . 1 ，構建了用於核酸免疫的真核表達載體pcdna3 . 1 - cpti ；質粒大量提取和純化后，通過肌肉注射的方法免疫balb c小鼠，基因表達產物刺激小鼠機體產生免疫反應，從而獲得了抗cpti蛋白的抗體。分享友人