酶標記探針 的英文怎麼說

中文拼音 [biāojìtànzhēn]
酶標記探針 英文
enzyme labelled probe
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 標 : Ⅰ名詞1 [書面語] （樹梢） treetop; the tip of a tree2 （枝節或表面） symptom; outside appearance; ...
  • 記 : Ⅰ動詞1 （把印象保持在腦子里） remember; bear in mind; commit to memory 2 （記錄; 記載;登記） writ...
  • 探 : Ⅰ動詞1 （試圖發現） try to find out; explore; sound 2 （看望） call on; visit; see 3 （向前伸出）...
  • 針 : Ⅰ名詞1 （縫衣物用的工具） needle 2 （細長像針的東西） needle like things 3 （針劑） injection; sh...
  • 標記 : （標志; 記號; 貨物標記） tab; sign; stamp; peg; label; mark; flag; blip; notation; fleck; track; ...
  • 探針 : probe; sound; filling fork; feeler; explorer; probing pin; touch needle; wire probe
  1. Our previous studies showed existence of apoplast cam in the plant cell and cam had many extracellular functions. so it supposed cam may be one of important extracellular polypeptides and trigger the intracellular signal transduction by binding the receptor. in this study, radiolabelled ligand is used to investigate the binding characteristic of cam and a. thaliana protoplasts. and chemical crosslinking is employed to explore binding proteins in the membrane. at first, ( 35 ) ~ s - cam was produced using ( 35 ) ~ s - labeled amino acid mixture in e. coli. sds - page and autoradiograph indicated high - purified, high - specific radioactivity ( 35 ) ~ s - cam was obtained. electrophoresis of ( 35 ) ~ s - cam is the same as that of unlabeled cam with ca ~ ( 2 + ) or egta ; a quatitive of protoplasts was prepared by enzymolysis

    首先，用~ （ 35 ） s標記的氨基酸混合物喂養工程菌成功地制備了~ （ 35 ） s標記的擬南芥鈣調素（ ~ （ 35 ） s - cam ） ， ~ （ 35 ） s - cam純度高、放射活度高、 ca ~ （ 2 + ）與egta存在時的電泳行為與未標記cam相同，可作為一種高靈敏性的探針用於進行受體學分析實驗；用擬南芥種子誘導愈傷，通過酶解制備了大量原生質體。
  2. Along with the development of the cytobiology and the molecular biology, and thoroughly research of the biophysics, the biochemistry, the genetics and immunology, it has cultivated the modem biological technology, such al genetic engineering, cellular engineering, enzyme engineering, fermentation engineering and so on, to change biology characteristic to carry on the material transformation, has formed the front biological examination technology : the dna probe, the pcr technology, the molecular mark, the bioluminescence technology, genechip technology and so on the widespread application of these advanced biotechnologies in dairy industry baa impelled the dairying technical transformation, and has been having vital significance to dairy production, research and dairy product security

    摘要隨著細胞生物學和分子生物學的發展及對生物物理、生物化學、遺傳學和免疫學研究的深入，培育了基因工程、細胞工程、酶工程、發酵工程等改變生物特性進行物質轉化的現代生物技術，形成了dna探針、 pcr技術、分子標記、生物熒光技術、基因晶元技術等前沿性的生物檢測技術，其在乳品工業中的廣泛應用，推動了乳業的技術變革，對乳品生產、研究和乳品安全意義重大。
  3. Selected one of the 14 strains - s93, s93 dna was digested partially with sau3a i and 2 ~ 3kb fragments were collected and inserted into puc 18, then transformed into dh5 a. filtering the clone with hybridization in situ, a 1 kb frament clone has been cloned

    使用sau3ai對基因組dna進行不完全酶切，回收2 3kb片段，與puc18質粒連接轉化大腸桿菌，利用地高辛標記探針，使用菌落原位雜交篩選轉化子；篩選到包含有約1kb外源片段的轉化子。
  4. Anti - igg antibody was immobilized onto the surface of gold electrode modified with a thin layer of protein a. biotin labeled anti - igg antibody and hrp - streptavidin were premixed to form network complex to be used as probes for amplifying the sensing response of antibody - antigen interaction

    抗體通過單層蛋白a固定到金電極表面，預先處理好的生物素化抗體和辣根過氧化物酶（ hrp ）標記的親和素形成的網狀復合物作為免疫反應信號的放大探針。
  5. Halobacillus trueperi accumulates glycine betaine under condition of high osmolarity. a partial fragment of the glycine betaine transporter beth gene was obtained from the genome of h, trueperi with degenerate primers. through southern blot hybridization and inverse pcr, a 5. 1 kb ecori fragment containing the beth gene was sequenced

    將擴增片段用地高辛標記成探針，與用不同限制性內切酶完全酶切的h . trueperi總dna片段作southern雜交，結果顯示在ecori酶切片段的5 . 1kb處有陽性信號。
  6. With h. trueperi genomic dna and degenerate primers, a 560 bp pcr fragment was obtained and labeled as a probe. after h. trueperi genomic dna was digested with different endonucleases, southern blot result showed a 2. 6 kb positive fragment digested by ecori and ipcr was carried out to obtain the flanking sequence

    將擴增片段用地高辛標記成探針，與用不同限制性內切酶完全酶切的h . trueperi總dna片段作southem雜交，結果顯示在ecori酶切片段的2 . 6kb處有陽性信號。
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