酶法分析 的英文怎麼說

中文拼音 [fǎfēnxī]
酶法分析 英文
enzymatic analysis
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 法 : Ⅰ名詞1 （由國家制定或認可的行為規則的總稱） law 2 （方法; 方式） way; method; mode; means 3 （標...
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 析 : Ⅰ動詞1. （分開; 散開） divide; separate 2. （分析） analyse; dissect; resolve Ⅱ名詞（姓氏） a surname
  1. It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity

    本研究中，木耳屬2個種的2個菌株在its區域表現出較高的保守性， 4種限制型內切酶的酶切圖譜沒有顯示出多態性；增加內切酶種類及供試菌株數量，有可能獲得具有多態性的限制性內切酶酶切圖譜； 9本實驗中， its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異，擴增片段長度均為650bp左右； 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析，紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法，兩者的關系是相輔相成，互為驗證
  2. In this study, the stem segments of new shoot with axillary buds of well - growth tetraploid black locust trees were used as explants. the effects of different basic mediums, different hormone kinds and their concentrations ratios, different sucrose concentrations on calli induction, buds differentiation and rooting in the process of establishment of high frequency regeneration system of tetraploid black locust were studied. on the base of high frequency regeneration system, the effects of various factors on transformation efficiency of badh mediated by agrobacterium tumefaciens were discussed in the light of gus histochemical assays

    本實驗首先以生長良好的四倍體刺槐優株上當年生新梢的帶腋芽莖段為外植體，研究了在四倍體刺槐高頻再生體系的建立過程中不同基本培養基、不同激素濃度及其配比、不同蔗糖濃度對愈傷組織的誘導、芽的分化及生根的影響；然後在得到高頻再生體系的基礎上，通過農桿菌介導法轉化甜菜堿醛脫氫酶（ badh ）基因，以gus染色組織分析為依據探討了影響轉化效率的各種因素，建立了高效、可重復的基因轉化體系，為四倍體刺槐目的基因的導入打下了基礎。
  3. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  4. Methods the influence of fluoride on the rat first passage osteoblast were evaluated by histochemistry, enzymehistochemistry and electron microanalysis in vitro

    方法應用組織化學、酶組織化學和電子顯微分析等手段觀察氟對體外培養的鼠第一代成骨細胞的影晌。
  5. We report here the cloning of a homolog of this gene from dunaliella salina. this cloned gene produces as protein with photolyase activity when expressd in escherichia coli

    運用blast方法對est序列進行分析，初步預測該est序列為光裂合酶藍光受體蛋白質家族中一個成員的部分cdna 。
  6. Some progresses in the research of enzyme - linked immunoassay

    酶聯免疫分析法研究進展
  7. The separation of nattokinase in leavening agent

    發酵液中納豆激酶鹽析法分離的研究
  8. Methods for quantitative analysis of lipase

    脂肪酶的定量分析方法
  9. Second, the population genetic structure and genetic diversity of e. mollis were studied by using allozyme eletrophoresis and the electrophoretic data for 6 loci from 3 populations being xiangning, yicheng and pinglu populations in shanxi were got. the level of polymorphism was relatively higher than that of the insect - pollinated outcrossing species ( he = 0. 375 )

    用等位酶電泳法和biosys - 2軟體對山西翅果油樹種群的遺傳結構和遺傳多樣性進行了研究，通過對3個種群的6個等位酶位點的電泳分析，結果表明： 5個位點為多態位點， 1個單態位點。
  10. In this article the methods for purification and assay for pectic enzymes were reviewed with 28 references

    本文對果膠酶分離純化手段及其分析方法進行了綜述。
  11. The purification and assay methods for pectic enzymes are differed with various strains

    由於不同菌種產生的果膠酶成分復雜程度不同，分離純化手段和分析方法也不相同。
  12. Methods for analysis of animal feeding stuffs - determination of soluble nitrogen content after treatment with pepsin in dilute hydrochloric acid

    動物飼料分析方法.第15部分:經含胃蛋白酶稀釋水解酸處理后溶解氮含量測定
  13. These results demonstrate that the heat dissipation ability in the transgenic plants decreases, suggesting that xanthophyll cycle has the function in photoprotection. besides, a dna minipreparation method suitable for screening and identification large amounts of transgenic plants was established. using this rapid and efficient method, one person can prepare d

    另外，還建立了一種適合於篩選轉基因植株的dna微量提取法，此方法操作快捷方便，一個人在一天內能制備50多個樣品， 100mg的植物鮮樣平均可獲得40卜g的dna ，提取的dna可直接用於pcr反應、酶切分析及southern分析。
  14. Then, we find out the write clones through the chemis hib. during the working, three probes which we used are from the published sequences of spidroin. after this, a genie library of subclone of dragline was constructed

    然後我們從構建蛛絲蛋白的亞克隆文庫著手，採用鳥槍法測序的策略，取從蜘蛛cosmid文庫中經過證實的陽性克隆scos - ds1 ，大，小為40kb左右，用cai ， eco47 ，和hinf三種酶分別對其進行酶解分析。
  15. The results showed mn and ni complexes possibly bind to dna by the mode of interaction, whereas zn complex possibly bind to dna by the modes of interaction and electrostatic binding. 5. in addition, we conjugated cleavage system with recognize system and analyzed joint products by hplc, which provide experimental basic for design of dual effects cleavage

    此外，本文還選用咖啡酸純品來突破切割體系與識別體系（用氨基臂修飾的寡聚脫氧核苷酸）的連接，並用高效液相色譜法分析其偶聯產物，為今後設計併合成一種具有特異識別和高效切割雙重功能的人工核酸酶提供了實驗基礎。
  16. A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge

    用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接；用連接產物轉化大腸桿菌jml09感受態細胞，置含amp 、 x - gal和iptg的lb平板上培養12 20小時；挑取白色菌落於選擇性培養基擴大培養，堿裂解法小量提取質粒dna ，並進行酶切分析鑒定，結果獲得整合有prvge基因的重組質粒pugedna ，並與其它prv分離株進行ge基因序列同源性分析。
  17. Ten isozymes and proteins were investigated by cellulose acetate membrane electrophoresis, the phylogenetic relationship among four groups of microtus fortis was analyzed based on the alleles frequency at these biochemical loci

    應用乙酸纖維素膜電泳法分析了四類東方田鼠的10種同工酶及蛋白，並觀察了它們在這些生化基因位點上的基因分佈特徵以研究它們之間的遺傳關系。
  18. The aggregate of egg white lysozyme molecules formed during the refolding procedure of denatured - reduced egg white lysozymes was analyzed by protein electrophoreses and high - performance size - exclusion chromatography

    本文利用蛋白電泳和高效凝膠排阻層析法分析了還原脈變性蛋白溶菌酶稀釋復性過程中的集聚體。
  19. The distribution and accumulation of three kinds of heavy metals including lead, copper and mercury in tissues and cells of several organs, and their effects on activity of digestive enzymes and alkaline phosphatase of macrobrachium nipponense were studied with light microscopy, transmission electron microscopy, cytochemical method, atom absorption spectrum, mass spectrum analysis and enzyme analytical method

    本文應用組織學、組織化學、透射電鏡、原子吸收光譜分析和酶學分析等方法，研究了鉛、銅、汞等三種重金屬在日本沼蝦各主要器官細胞內的分佈和積累以及對日本沼蝦消化酶和堿性磷酸酶活性的影響。
  20. The acetylcholinesterase membrane fixed by activated carrier had higher enzyme activity and longer life - span

    為建立快速的痕量有機磷類的酶法分析方法奠定了基礎。
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