酶消化法 的英文怎麼說
中文拼音 [xiāohuàfǎ]
酶消化法
英文
enzyme digestion-
In the first trial, combination of enzymatic digestion was used to prepare suspensions of spermatogenic cells from adult mouse testis, and then a modified discontinuous percoll gradient centrifugation method ( 15 %, 22 %, 30 %, 40 %, 50 %, 60 % ) was introduced to isolate spermatids from the cellular suspensions. the content of spermatids in each isolated fraction by percoll method was determined by morphology ( wright - giemsa stain ) and flow cytometry analysis, and the viability of spermatogenic cells was assessed by using eosin y exclusion test
在第一部分試驗中，首先利用連續3次組合酶消化成年小鼠睪丸制備睪丸細胞懸液，然後經6層非連續percoll梯度離心法（ 15 、 22 、 30 、 40 、 50和60 ）分離，通過形態學和流式細胞術鑒定南京醫科大學碩士學位論文各個percoll組分中精子細胞的含量，並以伊紅y排斥試驗測定細胞的存活率。The main study in this paper included as follows : the content and distribution of heavy metals in sediments and benthic organisms from the sewage stream in guangzhou city ; the acute toxicity and joint toxicity of mercury and selenium to swordtail fish ( xiphophorus helleri ) ; the damage of mercury to the indexes of antioxidant system in the gills and livers in swordtail ( including the measurement of the activities of total antioxidative capacity [ t ~ aoc ], superoxide dismutase [ sod ], glutathione peroxidase [ gsh - px ] and the concentration of malondiald - ehyde [ mda ] ) and the relief effects of selenium on it, as well as the physiological damage of mercury on the tissues, namely : the antagonistic effect of na + - k + ~ atpase activity on the tissues between selenite and mercury, and the ultrastructural damage under the exposure of mercury
研究內容主要有：廣州市河涌沉積物及底棲生物體內重金屬含量及評價；汞和硒對劍尾魚的急性毒性和聯合毒性及安全濃度的評價；汞對劍尾魚鰓和肝臟中抗氧化系統的毒性，包括對總抗氧化能力、超氧化物歧化酶、谷胱甘肽過氧化物酶活力及丙二醛含量的測定及硒對其保護作用；汞對劍尾魚組織生理毒性即：汞對na ~ + - k ~ + atpase活力的影響及硒的保護作用和汞和對劍尾魚組織超微結構的損傷等。以高氯酸?硝酸消化法和火焰原子吸收分光光度法測定了廣州市河涌沉積物和底棲生物中重金屬含量。To study the suitable method for cattle oviduct simple epithelium cells culture, the epithelium cells were isolated by cutting and 0. 25 % trypsinization, the exponential phase of growth cells vigor and growth velocity was determined by mtt method, the viable count was detected by the rejection experiment of trypanblau
摘要為探討適用於黃牛輸卵管單層上皮細胞的培養方法，採用機械剪取及0 . 25 %胰酶消化的方法分離獲得上皮細胞，取對數生長期細胞進行mtt比色實驗檢測細胞活力和生長速度；臺盼藍排斥試驗檢測活細胞數。The viable cells after counting with trypan blue dye exclusion were then transferred to culture flask containing dmem medium in a density of 1 10 ^ 6
方法無菌條件下，從6月齡紐西蘭白兔的膝關節囊內剪取滑膜組織，採用組織塊培養法和酶消化法分離滑膜細胞。In this experiment, radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process. methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0. 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al. thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum, and added to 50ml cell culture flasks
方法：胰島的分離參照王玲等的方法，每次實驗取新生3 - 5天sd大鼠，無菌條件下剖腹取出胰腺，剪切為0 . 5mm ~ 3的組織塊， v型膠原酶消化30min后，離心洗滌，懸浮於完全培養基，接種入50ml培養瓶，於5 co _ 2 、 95空氣條件下培養20h ，轉板純化，接種於96孔培養板培養24h ，按實驗要求進行實驗。The results as follows : 1 ) the primary culture of qinchuan - scalper skin cells could be derived by fragment of tissue dispersed and cold digested single cell, collegenase i ( 150iu ml - 1 " ) and trypsin ( 0. 05 % ) being used at the same time is best to dissociate qinchuan - scalper skin tissue, which is appropriate to obtain qinchuan - scalper skin cells
組織塊法、分散單細胞及dispase冷消化法單細胞均能獲得好的秦川牛皮膚組織原代培養物。其中150iu ? ml ~ （ - 1 ）膠原酶i與0 . 05胰蛋白酶（ 1 ： 1 ）同時應用能更好的分離秦川牛皮膚組織，是獲得秦川牛皮膚細胞的適宜方法。At last, ctab - dna and sds - dna methods are used in this experiment. on the basis of optimizing experiment procedure and pcr system of the materials, the initial reversion transcription system and orthologus gene cloning technique are established
對這3種方法比較后，確定本實驗採用ctab - dna酶消化法和sds - dna酶消化法提取白樺雄花芽組織的總rna 。Since there are more things in the bud tissue of the male flower, such as phenolic compounds, polysacchrides, proteins and some other secondary metabolites, three methods used to isolate rna are tested in this assay, which are the enzyme digesting methods of ctab, ctab - dna and sds - dna
克服了常規方法和試劑盒無法提取出富含酚類化合物、多糖和一些尚無法確定的次級代謝產物的白樺花芽組織rna的障礙，提出了3種白樺雄花芽組織rna的提取方法： ctab 、 ctab - dna酶消化法和sds - dna酶消化法。The smg cell of rats were isolated and purified by pancreation digestin and then were cultured and subculfured in dmem with 20 % fetal bovine serum
應用胰酶消化法進行頜下腺細胞的分離、純化及原代培養、傳代培養。Culture of rabbit osteoblasts digested by collagenase in dmem containing fetal bovine serum
含胎牛血清膠原酶消化法培養兔成骨細胞Conclusion the enzyme digestion procedure is a stable and reliable method to obtain bovine retinal pericytes
結論：酶消化法原代培養牛視網膜周細胞是一種穩定、可靠的方法。Methods using different techniques of collagenase digestion and discontinued density gradient centrifugation, the adult swine and rat islets were prepared
方法採用不同的膠原酶消化法及不連續密度梯度純化法。In this experiment, the method of trypsin digestion was improved, and that several kinds of mouse fibroblasts and porcine fibroblasts were successfully dissociated and cultured
實驗中改進了胰蛋白酶消化法，成功地分離和培養了多種鼠成纖維細胞和多種豬成纖維細胞。This research use high sensitive, special in situ pcr technology to determine the material which is treated by the paraffin wax and formalin fixation of different time, by detecting the genotype of mn blood group of the organizes slices. at the same time, we study the research of main influence factors, such as protease digestion, etc. we hope to set up a kind of steady, practical methods to detect the genotype of the material treated by paraffin wax and formlin, offer a kind of new detecting means for the forensic appraises and iditificition with individual material evidence
本研究應用靈敏度高、特異性好的原位pcr技術，測定福爾馬林固定不同時間的石蠟切片組織mn血型的基因型，並對蛋白酶消化時間等主要影響因素進行了初步的研究，以建立一種穩定、實用的檢測石蠟組織切片dna遺傳標記的方法，為法醫物證鑒定和個人識別提供一種新的檢測手段。Cells were centrifuged for 5min at 1000rpm and removed supernatant. the cell peller was resuspended in 1 ml culture media and divided into two glass flask. cell surface makers ( cd45, cd90 ) were detected by immunocy - tochemistry
當傳到第五代時，取一瓶mscs用胰酶消化，以8x10vcm 『濃度接種在六孔板中的蓋玻片上，應用免疫細胞化學方法檢測cd45人d90的表達。The fetal liver stem cells were isolated by collagenase digestion, gravity sedimentation and density gradient centrifugation, identified by immunocytochemistry and evaluated by flow cytometry for their proliferation condition
採用膠原酶消化、重力沉降及密度梯度離心方法分離人胎肝幹細胞，通過免疫細胞化學方法對其進行初步鑒定，以及應用流式細胞儀等對其生長狀況進行評估。In the current study, we tried to isolate liver progenitor cells from retrorsine - treated mouse. after long - term culture and purification, the pure epithelial cell population was established in cobblestone fashion with high nuclear - to - cytoplasm ratios
採用兩步膠原酶消化法從損傷后的再生肝中分離細胞，經過長期的培養和不斷的純化，最終在體外建立了形態均一的連續細胞系。( l ). cultivation of dermal fibroblast : dermal fibroblasts were harvested by using enzyme digest technique from child foreskin after circumcision. 3 - 4 generation of cells were used as work cells. ( 2 ) cultivation of dermal fibroblast on collagen - chitosan spongy membrane
真皮成纖維細胞的分離培養：取包皮環切術后的幼兒包皮酶消化法分離培養真皮成纖維細胞並傳代，以3 4代細胞作為工作細胞。All study aim is to lay a foundation for clinic appliance. methods : 1 ) hpfl was isolated by enzymatic digestion derived from rat fetal liver on ed13. 5d. furthermore, erythrocyte and other cell were removed from hpfl by erythrocyte - cracking solution and different attachment method
研究方法： 1 ）取ed13 . 5d的大鼠胎肝，酶消化法離散細胞，用紅細胞裂解液去除紅細胞，差速貼壁法去除其它細胞，接種于不同的基質和培養液中， mtt法比較不同培養液和培養基質對大鼠hpfl的體外生長影響。Methods : vascular endothelial cells, smooth muscle cells and fibroblast are respectively isolated from human umbilical veins by enzyme digestion and tissue plant methods, subcultured, purified and identified, etc. phase - contrast and electron microscopy was used to analyze the cells morphological characteristics
方法：分別採用酶消化法和組織塊法培養血管內皮細胞、血管平滑肌細胞及成纖維細胞，並進行三種細胞的傳代、純化、鑒定以及形態學觀察。分享友人