酶結構分析 的英文怎麼說

中文拼音 [jiēgòufēnxī]
酶結構分析 英文
enzymic structure analysis
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 結 : 結動詞（長出果實或種子） bear （fruit）; form （seed）
  • 構 : Ⅰ動詞1 （構造; 組合） construct; form; compose 2 （結成） fabricate; make up 3 （建造; 架屋） bui...
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 析 : Ⅰ動詞1. （分開; 散開） divide; separate 2. （分析） analyse; dissect; resolve Ⅱ名詞（姓氏） a surname
  • 結構 : 1 （各組成部分的搭配形式） structure; composition; construction; formation; constitution; fabric;...
  1. Abstract : several - aromaticamino ketones, which were designed according to some hypothetical models of the cyclooxygenase and 5 - lipoxygenase active sites, were synthesized by an amino exchange reaction. the structures of the eight new compounds were confirmed by ir, 1h - nmr and elemental analysis. the results of the pharmacological tests showed some of the investigated compounds had significant anti - inflammatory activity on croton oil - induced ear edema of mice

    文摘:根據環氧化酶、 5 -脂氧化酶活性中心結構模型設計了一組-芳胺酮類化合物，並用胺交換反應合成了這些化合物.經紅外光譜、核磁共振氫譜及元素分析證實了8個未見文獻報道的化合物的結構.藥理實驗結果顯示.部分受試化合物在巴豆油誘發小鼠足趾腫脹模型中表現出一定的抗炎活性
  2. Second, the population genetic structure and genetic diversity of e. mollis were studied by using allozyme eletrophoresis and the electrophoretic data for 6 loci from 3 populations being xiangning, yicheng and pinglu populations in shanxi were got. the level of polymorphism was relatively higher than that of the insect - pollinated outcrossing species ( he = 0. 375 )

    用等位酶電泳法和biosys - 2軟體對山西翅果油樹種群的遺傳結構和遺傳多樣性進行了研究，通過對3個種群的6個等位酶位點的電泳分析，結果表明： 5個位點為多態位點， 1個單態位點。
  3. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  4. Levels of fasting blood glucose and 24h urinary microcontent of albumin 24 h malb were determined dynamically ; the serum glycosyl hemoglobin hba1c was determined after the last medication ; the ultrastructural changes of kidney were observed by transmission electron microscope ; the expressions of collagen, fibronctin, laminin ln, and the ecm metabolism influencing factors, including mmp2, tissue inhibitor of metalloproteinase timp2, transfer growth factor 1 tgf 1 in renal tissue were detected by immunohistological chemistry and image collecting analytical system

    動態檢測各組大鼠空腹血糖fbg 24h尿微量白蛋白24h malb ，末次給藥后測定大鼠血漿糖化血紅蛋白hba1c透射電鏡觀察各組大鼠腎臟超微結構改變，應用免疫組化技術及圖像採集分析系統測定各組大鼠腎臟組織中型膠原c纖維連接蛋白fn層粘連蛋白ln的表達，測定影響ecm代謝的基質金屬蛋白酶2 mmp2基質金屬蛋白酶抑制劑2 timp2及轉化生長因子1 tgf 1的表達。
  5. By investigate the primary structure of strawberry ent - kaurene oxidase, we found that it has four conserve amino acid sequence. it suggested that these conserved regions are important in gene function

    對一級結構分析發現4段非常保守的區域，推測是在此酶行使功能時的重要組成部分。
  6. And the intron had a lot of gt repeated sequence. the dna and protein sequence of this gene was analyzed using the bioinformatics tools. two functional domains were found in the protein

    運用生物信息學手段對3一磷酸甘油脫氫酶基因核酸以及蛋白質序列做出了分析，發現這個基因編碼兩種功能的結構域，磷酸化酶結構域和3一磷酸甘油脫氫酶結構域。
  7. Ie protein have multiple functions, such as conversion of the host cell into a metabolically activate states, activation of viral early genes in the early phase, repression of their own transcription. ie 180 proteins can stimulate transcription from both homologous such as the icp4 of hsv ( herpes simplex virus ) and heterologous viral and cellular promoter such as - globe protein

    經分析偽狂犬病病毒立即早期蛋白（ immediate - earlyprotein180 ， ie180 ）的蛋白質結構，發現ie180具有轉錄激活因子的結構特徵，能與類rna聚合酶結合， ie180基因缺失突變體對sv40及cmv啟動子調控作用的研究，是考察ie180能否作為轉錄激活因子應用的關鍵。
  8. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  9. This research began with detecting the rpob gene mutations of 37 mycobacterium tuberculosis clinical isolates by polymerase chain reaction - single strand conformation polymorphism ( pcr - sscp ) to study the application value of this method as a new molecule testing method for drug susceptibility

    本研究首先通過聚合酶鏈反應?單鏈構象多態性分析技術對37株結核分枝桿菌臨床分離株的rpob基因進行突變檢測，目的在於探討pcr - sscp作為新的分子藥敏試驗方法的應用價值。
  10. The market occupation of retapase project has been over appraised and the market orientation is based on self - reference while less weight has been put on market survey ; the project fund comes inadequately from self - raised resources or by transferring the techniques. in the organization framework of the project, the conventional linear system is adopted, which inhibit imbibing the wisdom of majority ; on personnel management, the r & d professionals have been weighed much more against the management professionals and little effort has been put on building the professionals reservoir, while the personnel incentive tactics are not conducive to the long benefit of the project in addition, the cost management is maldeveloped and the project progress controlling has been neglected

    瑞特普酶項目屬生物制藥項目，本文對fdzj公司瑞特普酶項目的管理現狀進行了詳細分析，具體有待改進地方有：市場份額估計過高，市場定位本位化，市場調查注重不足；項目融資僅採用自籌資金及科技成果轉讓的方式；項目組織結構為傳統的直線制，不利於集思廣義，人員配備重研發人才，輕管理人才，人才儲備工作欠缺，人員激勵策略與項目長遠利益不銜接；成本管理欠缺，忽視進度管理等。
  11. Enzymatic synthesis and structure analysis of the monosubstituted derivative of - cyclomaltoheptaose

    環糊精的酶促合成和結構分析
  12. 374 - nt sequence analysis between nt 47 - 420 and restriction enzyme ( re ) clevage site mapping of f gene between nt 34 - 1682 were used to compare the 18 isolates for genetic analysis. a phylogenic tree was constructed based on the 374 - nt - sequence data of eighteen isolates in the study and 37 ndv reference strains from genbank and published resources

    通過dnastar軟體對f基因47 470nt間片段進行同源性分析比較並繪制了遺傳進化樹枝狀結構發生圖，結合334 1672nt間三種限制性內切酶（ re ： hinf ， bsto及rsa ）位點的分佈情況，確定了這些分離株的基因分類地位。
  13. The work on physical mapping of the chromosome of s. nanchangensis ns3226 was initiated. nearly a full set of chromosomal asei - bamhi fragments of s. nanchangensis ns3226 were cloned and used as probe to hybridized against its genomic library. thirty four asei linking cosmids were observed from 162 hybridizing cosmids and 20 of them showed no obvious overlapping each other by bamhi digestion, suggesting distinct identifications

    此外，還開展了南昌鏈黴菌ns3226染色體物理圖譜構建的前期研究工作：基本克隆到了南昌鏈黴菌ns3226染色體上全套的ase - bamh片段，以它們為探針從南昌鏈黴菌ns3226的基因文庫中釣到164個陽性克隆，並從中篩選到34個ase linkingcosmids ，用bamh進行初步的酶譜分析，結果表明其中有20個cosmids的bamh酶譜相互間沒有明顯的重疊性。
  14. Conclusion : by restriction enzyme secting, ligating, transforming, restriction enzyme analysis, and final dna sequencing, the pbd - i and pbd - ii gene were proved to be recombinated with the expression vector and the recombinated vector ppd - 1 and ppd2 were transformed successfully

    結論：經過酶切、連結，構建成重組質粒ppd上、 ppd上，再經轉化、抽提質粒及酶切分析，最後經dna測序證實， rticr擴增的pbd i 、 pbd 11基因與piflpdt 」 xsi表達載體構建成功。
  15. However, pprl protein has three potential functional motifs : neutral zinc metallopeptidase ( zinc - binding signature ), bacterial regulatory protein ( lacl family signature ), and duf955

    另外綜合幾個工具分析了該蛋白的結構域，發現了三個潛在的信號，它們分別是duf955 ， hth _ laci和zn依賴性肽酶。
  16. 2. sequence comparison of alpha - amylase gene of bombyx mori and bombyx mandarina the alpha - amylase gene structures of bombyx mori and bombyx mandarina are very similar, both comprised of five exons and four introns, with comparable length. the mean at contents of exons and introns are not much different

    家蠶和野桑蠶-澱粉酶基因序列比較分析家蠶和野桑蠶-澱粉酶基因具有相似的基因結構，都包含5個exon和4個intron ，各內含子、外顯子長度在家蠶和野桑蠶間較類似，外顯子和內含子at的平均值差異不大。
  17. One is phosphatase - like domain and the other is glycerol - 3 - phosphate dehydrogenase domain. the former domain may catalog the dephosphation of the glycerol - 3 - phosphate and the later presumably synthesize the glycerol - 3 - phosphate according to similarity searching, domain localization and structure comparison. these should give an evidence for the functional research of this gene

    通過相似性搜索，結構域定位以及結構比較分析我們認為磷酸化酶結構域很可能是催化3一磷酸甘油水解，而3一磷酸甘油脫氫酶結構域是催化3一磷酸甘油合成的，為這個基因功能的鑒定提供了根據
  18. The tree based on the results of allozyme and issr data shows that d. pleiantha is on one branch distinguished from all populations of d. versipelis

    根據等位酶分析和issrs結果構建的系統樹，相對於八角蓮各居群，六角蓮都單獨分為了一支。
  19. In this study, the recombinant plasmid pmd - 18t - pea - h3 was cleavaged with ncoi, xhoi and inserted into the expression vector pet - 28c and subsequently subjected to restriction endonuclease analysis and sequencing, the result indicated that the prokaryotic expression vector pet - 28c - pea - h3 was constructed successfully. after the expression plasmid was extracted and transformed into expression hosts bl21 ( de3 ) of e. coli, the transformed hosts were induced by iptg, bysds - page and elisa analysis of host protein. the expression of the objective gene was detected, and it could account for 16. 28 % of the total host protein. inclusion body was prepared from the incubating expression hosts induced by iptg

    同時將原核表達載體pet - 28c用nco ， xho雙酶切，回收酶切產物，將回收的酶切產物pea ， h3 ，載體進行連接，並轉入dh5感受態細胞內，培養12 - 18小時后，挑取陽性菌落，經nco ， xho雙酶切分析及pcr檢測，篩選到陽性克隆，其質粒測序結果表明成功地構建了毒性基因缺失的pea與人組蛋白h3融合基因的原核表達載體。
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