酶識別位點 的英文怎麼說
中文拼音 [zhìbiéwèidiǎn]
酶識別位點
英文
enzyme recognition site- 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
- 識 : 識Ⅰ動詞[書面語] （記） remember; commit to memory Ⅱ名詞1. [書面語] （記號） mark; sign 2. （姓氏） a surname
- 別 : 別動詞[方言] （改變） change （sb. 's opinion）
- 位 : Ⅰ名詞1 （所在或所佔的地方） place; location 2 （職位; 地位） position; post; status 3 （特指皇帝...
- 點 : Ⅰ名詞1 （液體的小滴） drop （of liquid） 2 （細小的痕跡） spot; dot; speck 3 （漢字的筆畫「、」）...
- 識別 : 1 （辯別; 辯認） discriminate; distinguish; discern; tell the difference; spot 2 [計算機] identif...
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The cbd tag of the fusion protein was cut by site - specific protease enterokinase at starting met of the target protein lt 27. it was released from the cbd fusion tag efficiently
利用融合蛋白中目的蛋白lt 27與cbdtag接頭處的腸激酶特異性識別位點，用腸激酶處理粗純化的融合蛋白，可將卜Many glycoproteins of lower and higher eucaryotes are attached to the plasma membrane by means of a glycosylphosphatidylinositol ( gpi ). gpi - anchored proteins are synthesized on membrane - bound ribosomes. upon translocation of the pro - protein across the endoplasmic reticulum membrane, gpi : protein transamidase ( gp1t ) recognize and removes the carboxy terminal gpi signal sequence and attaches a gpi molecule to the newly exposed carboxy terminal amino acid
Gpi化前體蛋白在依附於膜的核糖體上合成，當其易位穿過內質網（ er ）膜后，被gpi ：蛋白質轉酰胺基酶（ gpit ）識別， gpit在移走其羧基端gpi信號序列的同時將gpi分子連接至新生成的氨基酸位點上。Telomerase is a ribonucleoprotein complex ( rnp ) composed by its rna component and protein subunits. telomerase can synthesize telomeric dna onto chromosomal ends using its own rna component as a template, elongate the length of telomere, increase cell life and even induce cell immortalization
端粒酶（ telomerase ）是由端粒酶rna和蛋白質組成的一種核糖核蛋白復合物（ rnp ） 。端粒酶含有引物特異識別位點，能以自身rna為模板，逆轉錄合成端粒dna並加到染色體末端，使端粒延長，從而延長細胞的壽命甚至使其永生化。It is divided to extracellular and intracellular part by transmembrane domain. there are 13 n - glycosylation sites, 20 protein kinase c phosphorylation sites, 28 casein kinase ii phosphorylation sites, 4 tyrosine kinase phosphorylation sites and 15 n - myristoylation sites in the extracellular part of bt - r3 protein. an integrin recognition sequences rod lies in intracellular part of bt - r3 protein
跨膜區域（ tmd ）將它分為胞內和胞外兩個部分，它的胞外有13個潛在的糖基化位點， 20個蛋白激酶c的磷酸化位點， 28個酪蛋白激酶的磷酸化位點， 4個酪氨酸酶的磷酸化位點， 15個豆蔻（十四烷基）酰化位點；它的胞內有1個整合蛋白（ integrin ）識別位點。After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence
F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。Total rna was extracted from hepatic cells of mouse. a ecori and bamhi restriction sites were introduced into endostatin gene at specific primer f r, and endostatin was amplified by rt - pcr, this endostatin gene contained bamhi and ecori restriction sites at its 5 " and 3 " ends respectively
從小鼠肝臟細胞中提取總rna 。設計合成一對特異引物，分別帶有ecori和bamhi的限制性內切酶的識別位點。用rt - pcr法擴增endostatin的基因片段，在endostatin基因的兩側引入ecori和bamhi酶切位點。分享友人