酸純正度 的英文怎麼說
中文拼音 [suānchúnzhēngdù]
酸純正度
英文
sureechtheit fastness to acid spotting-
The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。In the process of preparing mullite powder, we use teos and al ( no3 ) 3 as raw materials. the powder we prepared has high purity and its grain radius is relatively small and is distributed equality. by this method, we prepared about 600 g mullite powder and it provided basis for the next experiment
在溶膠-凝膠法制備莫來石粉末過程中，我們以正硅酸乙酯和硝酸鋁為主要原料，採用該法制得了莫來石粉末，該粉末純度很高，粒徑較小並且分佈均勻，我們按該法制得了約600g莫來石粉料，為接下來的實驗打下了基礎。In this study, n - bulanol for defatted igy was superior to water dilution ( wd ). after igy was defatted wilh n - butanol, further purification of igy wilh sodium sulphate and ammonium sulphate sailing out were compared. as a result, the salting out procedure of ammonium sulphale was superior to sodium sulphate
蛋黃正丁醇去脂后應用飽和硫酸銨和飽和硫酸鈉兩種不同鹽析法純化igy進行比較，飽和硫酸銨略好於飽和硫酸鈉鹽析法，其純化后所獲得的產量達9 . 5mgigy / ml卵黃，天津醫科大學碩士學位論文純度94
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