重構胚 的英文怎麼說

中文拼音 [zhònggòupēi]
重構胚 英文
reconstructed embryo
  • : 重Ⅰ名詞(重量; 分量) weight Ⅱ動詞(重視) lay [place put] stress on; place value upon; attach im...
  • : Ⅰ動詞1 (構造; 組合) construct; form; compose 2 (結成) fabricate; make up 3 (建造; 架屋) bui...
  • : 名詞[生物學] (初期發育的生物體) embryo
  • 重構 : bpr
  1. Chromosome analysis confirmed that blastocyst cells were derived from es donor cell

    細胞經染色體分析,證實其染色體來源於小鼠胎幹細胞。
  2. 6 - dmap + ccb + ethanol can effectively activate the reconstructed rat - mouse embryos. 5 ) 225 reconstructed embryos from 2 - cell to morula were transferred into 46 recipients

    23 l6 dmap乙醇ccb能有效地激活種間核移植重構胚
  3. The results show that the development rates of morula treated with serum starvation for 3d, 4d, 5d are higher apparently than nocodazole group and control group ( p < 0. 05 )

    直接注射法建的核移植胎的桑堪發育率( 25 95 )高於電融合法( 16 07 ) ,統計學分析差異顯著( p 0
  4. 4 ) after the activated nuclear transfer embryos had been cultured in m199 ^ and czbm for 48h, the cleavage rate can reach at 16. 41 % and 17. 41 %, and the morula rate were 7. 46 %, % and 6. 74 %, respectively

    08融合率:血請饑餓法和nocodazole法處理的供體細胞建的胎,經6 dmap乙醇ccb激活,分別獲得17 33o和17 02o的卵裂率以及6
  5. These observations show that reprogramming is easier in interspecific embryos reconstructed with es cells than that reconstructed with somatic cells, and that es cells have the higher ability to direct the reconstructed embryo development normally than fibroblast cells. oocytes were reconstructed with outbreeding kunming albino mouse es cells and enucleated rabbit oocytes, and the effects of the passages of es cells and 6 - dmap on the development of interspecific reconstructed oocytes were analyzed. the interspecific reconstructed es - rabbit oocytes were activated either by combined two set electric pulses and 6 - dmap or by two set electric pulses

    以上結果顯示, 6 dmap能增加胎幹細胞異種卵的卵裂率,對卵囊的發育率影響不大;高培養代數和低培養代數的胎幹細胞用於異種核移植不影響異種卵的卵裂率和囊發育率;用胎幹細胞作為供核細胞;比體細胞作為供核細胞所建的異種重構胚更容易進行編程,並且胎于細胞指導異種克隆胎正常發育的能力強于體細胞。
  6. Then the zona and polar body of oocytes treated with drug were removed by transferring into the m2 containing 0. 5 % protease. the mouse fetal fibroblast cells were glued to the oocytes memb

    該結果與重構胚的體外發育相符,表明正是這些己恢復微組裝功能的化學去核卵母細胞才對其供體核具有編程的支持能力。
  7. Since the success of dolly, the first cloned sheep with the adult somatic cells as karyoplast donor, new approaches have been developed for nuclear transfer technology. here we describe a handmade cloning method which combines the chemical induced enuleation and zona - free technology in embryo culture. enuleated oocytes were derived by exposing the oocytes to demecolcine and cytoheximide supplemented mdium sequently and its chromosome was depleted to the first polar body

    將培養10h的化學去核卵母細胞與供體成纖維細胞融合后lh 、 2h 、 3h ,分別有77 . 6 % 、 70 . 6 % 、 58 . 9 %重構胚的染色質發生凝集,其餘胎的染色體則處于原核期;而只在融合后3h , 27 . 9 %重構胚被標記出組裝的紡錘體,且其中的同源染色體己經分離。
  8. 2 ) the rat - mouse reconstructed embryos activated were been cultured in the maturation mediums of tcm199, ml6, czb and czbm ( czb modified ) for 48h

    直接注射法和融合法建的桑椹發育率分別可達9 . 84 、 9 . 48 。
  9. 2 ) we got 55. 08 % of fusion rate when reconstructed embryos were fused with 1500 - 1800v / cm voltage and 50ms impulse. in addition, a same result can be obtained with low voltage + length impulse or strong voltage + short impulse

    41的卵母細胞達到成熟:在1500 1800v cm , 30 40sin條件下,大鼠牛核移植重構胚能達到50廠0 55
  10. There was no significant difference between the fusion rates of reconstructed embryos. the cleavage rate of embryos reconstructed with c57bl / 6j oocytes was significant high than that of kunming albino, but the former only developed to 4 - cell stage ; the later could develop to blastocyts. it is implied that the oocytes of inbred mouse cannot support the long - term development of kunming es cell

    兩種重構胚的融合率之間沒有顯著差異( p 0 . 05 ) , c57bl 6j重構胚的卵裂率( 60 . 5 )明顯高於昆明白小鼠( 33 . 7 ) ,但是前者只發育到4細胞期,而後者可以發育到桑椹期和囊期( 2 . 3 ) 。
  11. Meanwhile, there was different in the development of embryos reconstructed with various strain oocytes. the activation methods, passages and types of es cells were comparing in mouse cloning

    胎幹細胞異種克隆中,比較了激活重構胚的不同方法、不同代數胎幹細胞以及不同類型的供核細胞對異種重構胚的影響。
  12. Two types of fibroblast cells were used as donor control, one derived from ear skin of an adult kunming albino mouse, and the other derived from a mouse fetus. three types of cells were transferred into perivitelline space under zona pellucida of rabbit oocytes respectively. the reconstructed oocytes were fused and activated by electric pulses, and cultured in vitro

    用二次電脈沖加6 - dmap聯合激活或單獨用二次電脈沖激活小鼠胎幹細胞-兔卵母細胞異種卵時,聯合激活組的卵裂率是86 . 2 ,明顯高於單用二次電脈沖組( 64 . 2 , p 0 . 05 ) ,囊形成率分別為17 . 0和13 . 4 ,兩組間沒有顯著差異( p 0 . 05 ) 。
  13. The ovaries were examined and the oviducts and uteri were flushed in vitro by laparotomy for maturated oocytes recovery. the results were as follows : in comparison with control group, fsh group and fsh + 30 % pvp group are more efficient ( p < 0. 01 ), whereas the two treatment groups in equal dosage have no significant difference ( p > 0. 05 )

    76 ( p 0刀5 ) ; nocodazole處理組與對照組卵裂率差異顯著( p 0刀5 ) ,而桑堪發育率無顯著差異t o刀引;經川、叩、扣血清饑餓處理建的重構胚,卵裂率和桑堪發育率無顯著差異o 0刀引。
  14. There were great effects of the genetic constitution of es cell on the development of embryos reconstructed with es cells in intraspecies

    在以胎幹細胞為核供體,進行同種克隆時,胎幹細胞的遺傳內涵對重構胚的發育有很大影響。
  15. In order to know if the genetic constitution of recipient oocytes influenced the development of embryos reconstructed from es cell, oocytes of c57bl / 6j and kunming albino were used as recipient, respectively

    為了了解受體卵的遺傳內涵是否對胎幹細胞重構胚發育也會產生影響,分別以c57bl 6j和昆明白小鼠的m期去核卵為受體,胎幹細胞重構胚
  16. Superovulation was induced by injection intramuscular of different dosages of fsh once daily for 5 days or fsh + 30 % pvp once, and then injection intravenous of 100 iu hcg on the 6th day

    經血清饑餓3d 、 4d 、 sd同步化處理建的,其桑堪發育率分別為20 22 、 18
  17. 3 ) the donor of sd rat fibroblast cells, which had been treated with serum starvation for 3 - 5d, the 10 u g / ml of nocodazole and control, were used to recombine rat - mouse nuclear transfer embryos

    未經處理、血清饑餓3 - 5d 、 10 5 g mlnocodazole處理的大鼠成纖第四軍醫大學碩士學位論文雛細胞做供體胎,分別得到4 14 、 6 45 、 2
  18. This study were designed to investigate development potency of the reconstructed embryos of black bear - cat inter - species nuclear transfer in vitro / vivo, to develop the principle and technology of somatic nuclear transplantation in inter - species of mammalian and to study the mechanism of coordination between cell nuclear and cytoplasm. somatic nuclear transplantation technology has been used to establish stem cell from different animals, which can provide materials to treat various diseases

    本研究運用體細胞核移植的原理和技術,以六月齡雌性黑熊的成纖維細胞為供核體,去核未受精家貓成熟卵母細胞為受體,建種間核移植胎,研究種問體細胞核移植胎的建方法和胎的體內外發育潛能及其影響因素。
  19. In order to establish the technique of isolating es cell, and test the developmental ability of reconstructed embryos derived from es cell and oocytes of intra - or inter - species, mouse es cell were isolated from mouse blastocysts, and were used as donor nuclear, and were transferred into oocytes of various strains. the factors that influenced the development of reconstructed oocytes were determined. the developmental ability of embryos reconstructed with mouse es cells and rabbit oocytes was observed and analyzed

    為了建立胎幹細胞分離技術及了解其在同種和異種卵母細胞中的發育能力,首先從小鼠囊分離胎幹細胞,並以它作為核供體,移植到同種不同品系去核的小鼠卵母細胞內,觀察不同品系的卵母細胞對胎幹細胞重構胚發育的影響。
  20. Abnormal development and expressing characteristics of quot; imprinting gene quot; in reconstituted embryos from porcine somatic nuclear transfer

    豬體細胞核移植重構胚發育異常及印跡基因表達特徵研究的進展
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