重疊克隆 的英文怎麼說

中文拼音 [zhòngdiěkèlōng]
重疊克隆 英文
overlapping clones
  • 重 : 重Ⅰ名詞（重量; 分量） weight Ⅱ動詞（重視） lay [place put] stress on; place value upon; attach im...
  • 克 : 克i 動詞1 （能） can; be able to 2 （克服; 克制） restrain; control 3 （攻下據點; 戰勝） overcome...
  • 隆 : 隆Ⅰ形容詞1 （盛大） grand2 （興盛） prosperous; flourishing; thriving 3 （深厚; 程度深） deep; in...
  • 重疊 : 1 （一層層堆積） one on top of another; overlapping 2 [電學] [計算機] overlap; overlay; superimpo...
  1. In summary, we constructed and characterized a bac library of silky, a unique chinese native breed of chicken. the library has high genome coverage ( 13 - fold ), chimerism ( 6 % ) and overlapped bacs, which made it a valuable resource to complete chicken physical map, study functional genes and construct bac contigs. we analyzed the whole genomic sequence and snps of tyrp1 and found that silky tyrp1 is different with other breeds both in microsatellite and transcription regulation site

    中國農業人學博卜學位論文摘要本研究構建了中國特有雞種絲羽鳥骨雞基因組bac文庫並進行了文庫質量鑒定，它所具有的13倍高基因組覆蓋率、 6 %的嵌合率和部分重疊的克隆使其成為完善雞的基因組圖譜、研究基因功能和構建bac重疊群的優質資源。
  2. After analysis fingerprinting of 16 bac clones. we obtained a 387kb bac contig containing 13 bacs

    通過bac指紋分析，構建起包含13個克隆，覆蓋了387kb的bac重疊群。
  3. Pfge analysis of 6 ecor ] digested bacs clones which were derived from the library and contained lmbr1 sequence proved the overlap among the bacs

    對篩選得到的6個含lmbr1序列的bac克隆酶切分析的結果表明bac克隆之間是相互重疊的。
  4. It has made the strong basis for further studying mechanism of replication, virulence and determinant, attenuation, pathogenesis, functions of genetic products, specific diagnosis, cell and host tropism, development of dna vaccine and marker vaccine of csfv, and provided an excellent tool for molecular virology. main research contents include : based on published nucleotide sequences of csfv and by the help of computer analysis software, high conservative regions and single restriction enzyme sites of genome were selected. utilizing rt - pcr and nested - pcr techniques, 7 overlapping cdna fragments covering the full genome of csfv c - strain were successfully amplified

    中國豬瘟兔化毒（脾淋毒）基因組cdna文庫的構建、序列分析：根據已發表的豬瘟病毒（ csfv ）核苷酸序列，藉助計算機軟體分析，選擇高保守區段和基因組中的單一限制性酶切位點，利用rt - pcr及nested - pcr和helf - nestedpcr技術，成功地擴增出了覆蓋c -株全基因組的7個cdna重疊片段f1 f7 ，分別克隆到pmd - 18t或pgem - teasy載體進行測序后，拼接出了其核苷酸序列。
  5. The work on physical mapping of the chromosome of s. nanchangensis ns3226 was initiated. nearly a full set of chromosomal asei - bamhi fragments of s. nanchangensis ns3226 were cloned and used as probe to hybridized against its genomic library. thirty four asei linking cosmids were observed from 162 hybridizing cosmids and 20 of them showed no obvious overlapping each other by bamhi digestion, suggesting distinct identifications

    此外，還開展了南昌鏈黴菌ns3226染色體物理圖譜構建的前期研究工作：基本克隆到了南昌鏈黴菌ns3226染色體上全套的ase - bamh片段，以它們為探針從南昌鏈黴菌ns3226的基因文庫中釣到164個陽性克隆，並從中篩選到34個ase linkingcosmids ，用bamh進行初步的酶譜分析，結果表明其中有20個cosmids的bamh酶譜相互間沒有明顯的重疊性。
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