重組核 的英文怎麼說

中文拼音 [zhòng]
重組核 英文
restitution nucleus
  • : 重Ⅰ名詞(重量; 分量) weight Ⅱ動詞(重視) lay [place put] stress on; place value upon; attach im...
  • : Ⅰ名詞1 (由不多的人員組成的單位) group 2 (姓氏) a surname Ⅱ動詞(組織) organize; form Ⅲ量詞(...
  • : 核構詞成分。
  • 重組 : bpr
  1. The second section described the accounting issues brought by cyber economy environment including the impact to the accounting suppose, accounting target, the content, the means, the process of accounting checking and the impact to the accounting information system. the third section described the ideas of the innovation of cyber economy environment emphases on the accounting business process reengineering, accounting information system innovation, assets affirmance, accounting settlement under the cyber economy environment. on basis of the third section, the fourth section analyzed the cyber accounting ' s origins, developme nt, characteristic and problems occurred in the development of the cyber accounting

    本文共分四章,第一章論述了網路經濟環境下會計環境的變遷,分析了網路經濟的產生與特點,概述了電子商務及虛擬企業的特徵,並指出對企業的織、生產、管理環境帶來的巨大變化;第二章闡述了網路經濟環境引發的會計問題,包括對會計假設、會計目標、會計算內容、方法、流程及會計信息系統的影響;第三章提出了對網路經濟環境下的會計創新的設想,點論述了網路經濟環境下的會計業務流程與會計信息系統的變革,網路經濟環境下的資產與會計確認,以及會計結算等方面的創新設想;在第三章討論內容的基礎上,第四章具體分析了網路會計的產生、發展及特點,並分析了網路會計發展過程中存在的主要問題,針對這些問題,提出了相應的對策。
  2. There are two main types of nuclear division : mitosis, which results in daughter cells identical to their parents ; and meiosis ( reduction division ), which produces daughter cells which have half the number of chromosomes of their parent cells, and in which the genetic material has been recombined

    分裂有兩種主要的類型:一種為有絲分裂,即子代細胞與親本相同,另一種為減數分裂,其產生的子細胞所含的染色體數為親本染色體數的一半,基因在分裂過程中發生了
  3. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。
  4. In this study five recombinant plasmids containing hn ( hemagglutinin - neuraminidase ) genes of ndv ( newcastle disease virus ) were constructed, hn genes were amplified by reverse transcriptase - polymerase chain reaction ( rt - pcr ) and were inserted directly into eukaryotic expression plasmid pcdna3

    應用反轉錄-聚合酶鏈反應( rt - pcr )獲得兩株新城疫病毒( ndv )血凝素-神經氨酸酶( hn )基因cdna ,然後定向插入真表達質粒pcdna3中,構建了含hn基因的質粒。
  5. Conclusions : prokaryotic and eukaryocytic expression plasmids of the shortened hepatitis b surface antigen were successfully constracted, and the target proteins expressed by iptg induced in escherichia coli. as well as in eukaryocyte ( hepg2 and cos - 7 ), then their antigenity were detected

    結論:截短的乙型肝炎表面抗原分子的原和真表達』質粒成功被構建及分別在人腸桿菌efl得到誘導表達和存貞細胞ifj表達,並檢測劍其表達產物的抗原特性。
  6. Porcine transmissible gastroenteristis is an importan contagious disease endangering the development of swine. in other to establish a rapid diagnosis method and provide effective immunogenic products, the nucleoprotein ( n ) gene of porcine transmissible gastroenteristis virus ( tgev ) was cloned. expressed and its expressed product was purified

    為建立對豬傳染性胃腸炎快速有效的診斷方法,並試圖在預防上提供有效的免疫制劑,本論文首次在我國對豬傳染性胃腸炎病毒衣殼蛋白基因進行了克隆、鑒定、表達及重組核蛋白的純化;並在細胞上對重組核衣殼蛋白抗體的中和效力進行了測定。
  7. The results show that the development rates of morula treated with serum starvation for 3d, 4d, 5d are higher apparently than nocodazole group and control group ( p < 0. 05 )

    直接注射法構建的移植胚胎的桑堪胚發育率( 25 95 )高於電融合法( 16 07 ) ,統計學分析差異顯著( p 0
  8. This fund will be adopted " core - satellitic investment is politic ", " core " be in showing in its investment is combined not the grail of invest in of stock capital fund under 80 is blue prepare, take the kind that buys hold ; " satellite " the part adopts a variety of strategy, invest in is new, in pannikin, asset recombines board piece, derive a tool to wait

    該基金將採取「心衛星投資策略」 , 「心」是指在其投資合中不低於80的股票資產投資于大盤藍籌股,並採取買入持有的方式; 「衛星」部分則採取多種策略,投資于新股、中小盤股、資產板塊、衍生工具等。
  9. Marek " s disease virus ( mdv ) phosphoprotein 24 ( pp24 ) gene was amplified from md11 strain by polymerase chain reaction ( pcr ). then we cloned it into the downstream of gst gene according to the right open reading frame ( orf ) in pgex - 6p - l vector

    本研究將型mdvmd11株的pp24基因的完整orf克隆入原表達載體pgex - 6p - 1中,質粒pgex - pp24轉化bl21宿主菌后,經iptg誘導表達。
  10. The recombinant expression plasmids prt - p450nor and pet - p450nor were constructed by inserting p450nor gene into the bamh i / hind iii site of the prokaryotic expression vector prset and pet28, then they were transformed into e. coli bl21

    本研究將已克隆的真菌細胞色素p450nor基因插入原表達質粒載體prset和pet28的bamhi / hind位點,成功構建表達質粒prt - p450nor和pet - p450nor ,並轉化到e . colibl21 。
  11. And at the same time, enterprises should firmly realign and regulate themselves around the raise of their core competitive power

    並圍繞提高心競爭力對企業進行堅決的和調整。
  12. ( genbank submission no. ay190323 ) eukaryotic recombined expression vector, pcdna3

    1和pegfp上,獲得真表達載體pcdna3
  13. The purpose of this study was to clone the major structural protein vp3 gene of gpv hl isolate after pcr, and express by protokaryotic and eukaryotic expressing system, then develop molecuiar diagnostic reagent of goose piaque and construct recombillat fowlpox vina life vector vaccine

    利用pcr方法擴增和克隆gpvh1分離株主要免疫原性蛋白基因vp3 ,並對其進行原和真表達,是建立小鵝瘟分子診斷方法、構建vp3基因禽痘病毒活載體疫苗的基礎,具有極為要理論和實踐意義。
  14. Study on in viro drug delivery and repairing large segmental infected bony defect with massive reconstituted bovine xenograft aided by calcium phosphate cement drug core

    磷酸鈣骨水泥載藥心的塊型合異種骨體內緩釋及修復兔長段感染性骨缺損的研究
  15. Construction of baculovirus transfer vectors for expression of core antigens of newcastle disease virus

    心抗原桿狀病毒轉移載體的構建
  16. Hi our study, dendritic cells ( dcs ) were derived from the cultivation of peripheral blood monocytes in vitro successfully. then to observe whether dcs transfected with carcinoembryonic antigen ( cea ) - vaccinia recombinant virus ( rv - cea ) can induces cytotoxic t lymphocyte - mediated cea - specific immunity in vitro

    體外成功地完成了cd14 +單細胞來源的樹突狀細胞( dc )的培養,並進一步研究人癌胚抗原痘苗病毒( rv - cea )轉染dc后體外誘導的cea特異性細胞免疫。
  17. This paper is a study on the expression of the protective gene of bont / a in the prokaryotic and eukaryotic. it indicates the possibility to produce the recombinant protein in quantities. it has also laid down a good foundation for the further research on vaccine or antibody of bont / a

    本論文進行了人工合成的a型肉毒毒素hc段基因在原和真表達系統中的表達研究,使制備大量bont a保護型抗原的蛋白成為可能,為進一步進行a型肉毒毒素的疫苗或抗體研究奠定了一定的基礎。
  18. After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s

    通過細胞的免疫化,細胞裂解物的sds - page電泳, westem - blot分析檢測目的基因的表達情況。免疫化結果顯示:質粒轉染的細胞質中有棕褐色顆粒,而空載體轉染細胞及正常細胞無此現象;細胞裂解物sds - page電泳結果顯示:只有質粒轉染的細胞在約38kd處有明顯的蛋白帶,這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致; western - blot分析結果顯示:約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清,成蟲蟲體可溶性抗原免疫兔血清, ts87基因原表達蛋白免疫兔血清( ts87血清)以及一株具保護性的旋毛蟲單抗特異識別。
  19. Effects of different electrofusion parameters on activation and early development of mouse embryos reconstructed by cumulus cell nuclear transfer

    不同電融合條件對小鼠卵丘細胞移植胚融合和早期發育的影響
  20. The research adopts that hu - - ifn gene were introduced into the nuclei of oocytes or cytoplasm of grass carp to develop anti - disease transgenic grass carp breeding researches, combing the adva ntag e of hu - - ifn gene and breeding by genetic engineering, with an aim of finding out an effective way of solving antivirus of hemorrhagic virus of carp completely. in research of transgenic fish, hu - - ifn gene ( recombination gene ) is cutdown and introduced into the nuclei of oocytes or cytoplasm of grass carp at one - cell or two - cell stage via micyoinjection by narashige micyoinjection apparatus

    本研究的目的在於以人的-干擾素基因( ifn - )作為目的基因,與鯉魚-肌動蛋白基因啟動子在體外,利用原顯微注射轉基因技術將人-干擾素基因導入草魚基因而開展的抗病轉基因草魚育種研究,其結合了干擾素和基因工程育種抗草魚出血病病毒的優點,以期獲得對草魚出血病具有天然抗性的轉基因魚,並在此基礎上培育出草魚抗病新品系。
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