重組的可溶性 的英文怎麼說

中文拼音 [zhòngzǔdekěróngxìng]
重組的可溶性 英文
recombinant soluble
  • 重 : 重Ⅰ名詞（重量; 分量） weight Ⅱ動詞（重視） lay [place put] stress on; place value upon; attach im...
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 的 : 4次方是 The fourth power of 2 is direction
  • 溶 : 動詞（溶化; 溶解） dissolve
  • 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
  • 重組 : bpr
  1. Because, this is of chinese herbal medicine outer the organization is stimulated suddenly by high fever, instantly constrictive, caky even, its protein is formed on cellular wall cannot go against the metamorphic layer that turn, prevent moisture development, organize interior to composition dissolves hard and be released, such, the decoct of medicaments active ingredient goes out rate reduce greatly, affect curative effect badly

    這是因為中草藥的外層組織忽然受到高熱刺激，立即緊縮，甚至凝固，其蛋白質便在細胞壁上形成不可逆轉的變性層，防止水分深入，組織內部成分難以溶解並釋放出來，這樣，藥物有效成分的煎出率大大降低，嚴重影響療效。
  2. A review of the development of organic semiconductor composite photo - conductive materials and devices was followed by a proposal of the researching theme in this thesis. the effects of fabrication arts such as solvents, gradient, interfacial layer and configurations on the photoconductive properties of the single - layer chlorodiane blue azo / tiopc composite photoreceptors were systematically studied in chapter ii. the results showed that the solvent played a decisive role in the multiphase and multicomponent system composed of two photogeneration species ( chlorodiane blue azo and tiopc ), a transporting material ( hydrozone ) and polymer binder ; 1, 4 - dioxane, as an amphiphilic solvent can effectively disperse and stabilize such multiphase and multicomponent systems, the derived photoreceptors presented improved photoconductive properties superior to those of dual - layer counterparts and demonstrated the evident synergetic enhancement and complementary effects ( eg

    結果表明：在由兩種光生材料氯丹藍偶氮和酞菁氧鈦、傳輸材料萘苯腙以及聚合物介質組成的多相多組分復合體系中，分散溶劑是至關重要的因素，二氧六環作為典型的雙親性溶劑，有效地分散和穩定了該多相多組分復合體系，得到的復合單層光導體的光敏性在整體上優于雙層光導體，復合材料在可見光和近紅外光區分別表現出的偶氮和酞菁氧鈦的光敏性（如azo / tiopc = 8 / 2 ， cgm / ctm / pc = 1 / 120 / 120時，具有明顯的互補效應；在近紅外光區明顯高於酞菁氧鈦與偶氮光敏性的線性加和，表現出協同增強正效應。
  3. After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s

    通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。
  4. Goat anti - human ige antibody were used as second antibody to make sure that the positive clones were ige related. through three cycles of screening, the inserted cdna fragments of the positive clones were amplified by pcr and sequenced. the results showed that the inserted cdna fragment from one clone was 1200 bp in length, with a orf of 507 bp which encoded 169 amino acids

    Sj43b pgex 6p 1重組質粒的誘導表達、表達產物的鄉寸和免疫學性質鑒定分析為獲得可溶性的rsj43b月6gsta蟲合蛋白，對不同iptg誘導劑濃度、誘導表達溫度和誘導表達時間等因素對融合蛋白可溶性表達的影響進行了觀察。
  5. This paper summarizes the distribution law and basic features of karst caves in zhejiang province, gives a brief introduction to the specific characteristics of 8 main karst caves, and maintains that scientific tourism is the only way for the sustainable development of tourist industry of karst cave scene : exploitation of karst caves tourist resources is still in the primary stage ; individual and combinative characteristics of every karst cave scene must be made prominent ; leaders of government at different levels as well as people in charge of karst cave scene should lay stress not only on economic benefit but also on societal benefit ; main operation stags of scientific tourism ; requirement of improving tourism taste will further development of tourism resources of karst cave

    摘要對浙江省巖溶洞穴的分佈規律與基本特徵進行了總結與概括，並就浙江省8個主要溶洞的個性特徵作了簡要的介紹，最後提出並論述了倡導科學旅遊是溶洞旅遊業可持續發展的必由之路：溶洞旅遊資源的開發目前仍處于初級階段；要突出每個溶洞景區的個性及其組合特點；各級黨政領導與溶洞景區負責人不僅要重視經濟效益，也要重視社會效益；科學旅遊的主要操作步驟；遊客對旅遊品位要求的提高也將進一步促進溶洞旅遊資源的深入開發。
  6. Lymphotoxin ( lt ) is a kind of pleiotropic lymphocyte - secreted cytokine which mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. in order to increase the antitumor activity of lymphotoxin and reduce its side effects, the recombinant plasmid pet36b - lt 27 was constructed to express soluble fusion protein cbd - lt 27. the active form of lt 27 could be collected directly with several simple steps by three kind of components on the expressed fusion protein

    本研究通過構建表達n端缺失27個氨基酸的淋巴毒素融合蛋白的重組質粒，在大腸桿菌中實現融合蛋白的可溶及分泌表達，同時利用表達載體上的幾種特殊序列經簡單的分離純化步驟直接獲得大量的有生物活性的淋巴毒素缺失體lt 27 ，為尋找一種高抗腫瘤活性、低臨床毒副作用的生物抗癌藥物進行了有效的探索。
  7. The protein has the immunogenicity of human calcitonin verified by western blot analysis. after optimization of condition, fusion protein was concentrated by ultrafiltration and cleaved by factor x a, then recombinant novel hct was obtained

    表達條件優化后，可溶性蛋白的表達量占胞內總蛋白的10 。經超濾濃縮后，融合蛋白在xa因子作用下產生重組的新型降鈣素，進一步分離純化得到該蛋白的初級純品。
  8. These include cell growth characteristics, expression levels, intracellular and extracellular expression, posttranslational modifications, and biological activity of the protein of interest, as well as regulatory issues in the production of therapeutic proteins. in addition, the selection of a particular expression system requires a cost breakdown in terms of process, design, and other economic considerations. section i : construction of pet22b ( + ) / hpk - 5 vector the hpk - 5 gene encoding 82 amino acid residues from c462 to c543 was recombined with the sequence of plasmid pet22b ( + ) for constructed a new expressed vector pet / hpk - 5

    方法在對hpk - 5 （ humanplasminogenkringle5 ， hpk - 5 ）因子的基因序列和蛋白質序列進行分析的基礎上，利用pcr技術分別構建其可溶性原核表達載體和不溶性原核表達載體；用pcr快速檢測法及其基因測序儀測序以鑒定pet22b hpk - 5和pbv220 hpk - 5重組質粒，用不同的感受態大腸桿菌（ e
  9. Huctla4 - lg, " one of the most potent in1n1unosuppressive molecules, is a soluble recombinant fusion protein ( molecu1ar weight of approximately 92 kd ) consisting of the extracellular domain of human ctla - 4 and a fragment ( hinge and constant region ) of the fc portion of human iggl. it strongly adheres to the b7 molecule to block the cd28 - mediated costimulatory signal, resulting in inhibition of in vitro and in vivo immune response

    可溶性重組融合蛋白huctla4 - ig是一種新穎的免疫抑制劑，分子量約為92kd ，由人ctla - 4分子的胞膜外區與人igg1的fc段（鉸鏈區與恆定區）融合形成，能牢固地與b7分子結合，阻斷cd28介導的共刺激信號，導致免疫反應的抑制。
  10. In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection

    此外，為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況，我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中，與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒，將其感染sf9細胞制備p1種子液，同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定，確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。
  11. The result demonstrated that the ctbvpl fusion gene was inserted into the c. reinhardtii chloroplast genome and after three rounds of spc selection the recombinant chloroplast dnas were the majority, and the wild - type chlorop last dnas

    W七sternblot和elisa免疫雜交分析表明ctbvpi融合蛋白在衣藻葉綠體中得到表達，表達量占可溶性總蛋白量的3一4 % （ lmg可溶性總蛋白含有30一40pg的重組蛋白） 。
  12. As an important innate immune system, and as an important arm of the humoral immune response, the complement system is immediately ready to target and eliminate virus particles, to lysis those virions that have lipoprotein membranes, or to prevent it from entering host cells, or to marker them for destruction by other branch of the immune response. at the same time, the host normal tissue are protected from damaging by complement through recognizing the regulators of complement activation ( rca ) expressed on self cells

    作為機體重要的天然免疫防禦系統及特異性體液免疫應答的重要效應系統，補體系統除了具有溶解、清除病毒等致病微生物，阻止病毒進入靶細胞，調理病毒的吞噬等重要功能外，還可通過「識別」自身組織細胞表面的補體活化調節蛋白來對自身細胞加以保護，使之不受侵害。
  13. After analyzing all kinds of uncertainty in determination, it was found that the uncertainty of results was mainly introduced by those of repeatability, working curve and standard solution, but other uncertainty was very trivial and can be neglected in real evaluation

    通過對測定過程中的各種不確定度分析，得到了測定結果的不確定度主要由測量的重復性、工作曲線和標準溶液引入的不確定度組成，其他方面引入的不確定度較小，在實際評定中可以忽略。
  14. 5. expression and detection of soluble mg7 scfv the positive dones possessing apparent inhibitory effect were individually transfected into eco / / hb2151 ; after induction by iptg, perplasmic extracts was prepared from the induced transfectant ; the yields of soluble mg7 scfv produced by the transfected eco / / hb2151 were evaluated via immunodoting assay

    7 u mg7scf64kilsrj將上述竟爭eu趴中篩蹦u的mo重組咱箔列莉b體陽性克隆感染hbzb2151大腸桿菌，經iptg誘導表達可溶11mg7schv ：牛憤iptgm菌的周質提取物。
  15. Though the expression level of bl21 ( de3 ) / pt221 - hyuh was lower than that of m15 / pqe60 - hyuh, the target protein of bl21 ( de3 ) / pt221 - hyuh was principally in soluble form while the objective protein of m15 / pqe60 - hyuh was principally in insoluble form. both of the products in the two strains showed biological activity, but the former is 2 times higher than the latter. the hyuc dna fragment was inserted into ppic3. 5k plasmid to construct the ppic3. 5k - hyuc recombinant plasmid which was then transduced into pichia pastoris gs115 cells after being linearized by bgl ii digestion

    結果表明， sds - page分析在50kd處有一較強的表達帶，融合有分子伴侶的重組菌株bl21 （ de3 ） pt221 - hyuh與非融合表達的重組菌株m15 pqe60 - hyuh相比，乙內酰脲水解酶的表達量低一些，但其表達蛋白主要以可溶性形式存在，而m15 pqe60 - hyuh中表達蛋白則主要以包含體形式存在，且前者的酶活性是後者的2倍多。
  16. Enhancement of expression efficiency and solubility of recombinant protein in gene engineering by a new method

    提高基因工程中重組蛋白質表達效率及可溶性產物的一種新方法
  17. The recombinant virus adhuctla4 - ig prepared in this study efliciently inltcted l - o2 " cells, and the infected cells expressed a - nd excreted soluble rccolllbina11t protein huctla4 - ig

    該重組病毒在體外能有效感染正常肝細胞株l - o2 ，受感染細胞能表達、分泌可溶性的重組融合蛋白huctla4 - ig ； 2
分享友人
分享到 Line

分享到臉書