重載列表 的英文怎麼說

中文拼音 [zhòngzǎilièbiǎo]
重載列表 英文
overload list
  • 重 : 重Ⅰ名詞（重量; 分量） weight Ⅱ動詞（重視） lay [place put] stress on; place value upon; attach im...
  • 載 : 載Ⅰ名詞（年） year : 一年半載 six to twelve months; six months to a year; 三年五載 three to five ...
  • 列 : Ⅰ動1 （排列） arrange; form a line; line up 2 （安排到某類事物之中） list; enter in a list Ⅱ名詞1...
  • 表 : Ⅰ名詞1 （外面;外表） outside; surface; external 2 （中表親戚） the relationship between the child...
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  3. The artificial antibacterial peptide - ceme gene was designed according to the codon with the highest frequency in pichia pastoris, then the ceme gene was integrated into the chromosome of pichia pastoris strain - gsl 15 by electroporation

    我們根據人工設計的新型抗菌肽ceme序列設計ceme基因，重組到酵母胞內表達型的整合載體phild2中，通過電轉化作用將ceme基因整合至酵母宿主gsll5染色體上。
  4. The plasmid plncxhlf which is combining with 2366bp human lactoferritin ( hlf ) cdna complete sequence and 800bp regulating sequence upsteam by the - lactoglobulin gene promoter is prepared by normal steps. then it is used in the following experiments. the recombinant plasmids plncxhlf were introduced into mouse mammary tumor cell line ma - 782 by liposome entrapment

    本研究將含有人乳鐵蛋白（ hlf ）基因2366bpcdna序列完整序列和800bp的奶山羊-乳球蛋白基因5 』端調控序列的乳腺特異表達重組載體plncxhlf按常規方法提取、純化、 pcr 、酶切鑒定后，用於細胞表達和精子介導轉基因動物的研究。
  5. On the basis of the study of the theory and appraise method on land use in the small towns from home and abroad, this paper at first conducts a deep study on the development and role of the small towns, indicating that its development has sawn an uneven development phrase and becomes a carrier of the enterprises, a pool of surplus laborers, a hub of material exchanges between the rural and urban areas, a base of spiritual civilization, an important way to achieve urbanization. second, it conducts a study on the situation and features and the problems the land use, indicating that the efficiency of the land use is low, which has a direct influence on the development of agriculture and the role of the small towns. and the study of the demand of the land indicates the shortage of land is serious, and the small town must rationally use the land and increases its intensive role and the economical efficiency to meet the demand

    在分析國內外已有關于小城鎮土地利用的理論與評價方法的基礎上，首先對小城鎮在我國的發展、地位和作用進行了深入的分析，判明我國小城鎮發展經歷了一個曲折向上的發展階段，已成為鄉鎮企業的載體，農村剩餘勞動力的蓄水池，城鄉物資交流的樞紐，農村精神文明的基地，是我國城市化的重要途徑；其次，對小城鎮土地資源利用現狀和特徵進行了探討，並對發展小城鎮建設導致的土地利用問題進行了剖析，表明目前我國大多數小城鎮土地效益和規模效益低下，佔用耕地過多，直接影響農業的發展，影響小城鎮的地位和作用；通過小城鎮土地供需分析研究表明，我國土地短缺十分嚴峻，小城鎮土地需求缺口較大，小城鎮必須合理利用現有土地，增強集約功能和土地經濟效益，從而緩解需求壓力；最後，論文通過運用特爾菲法，描述統計分析法、多元統計分析（主成分分析）法和系統分析法中的層次分析法（ ahp ）等一系列方法，結合定性和定量兩方面，從土地質量、土地資源數量與結構、土地經濟效益、環境效益、社會效益等五個方面進行分析，篩選、建立了土地資源利用評價指標體系，在因子評價的基礎上，建立了土地利用綜合評價模型，並給出了評價過程和方法。
  6. Therefore, blys, its receptor or related antagonists may find medical utility in the treatment of b cell disorders associated with autoimmunity, neoplasia, or immunodeficiency syndromes. in this study, epo signal peptide sequence and hsblys gene were linked by soe method. the fusion product was cloned into eukaryotic plasmids. pcdna3, pcdna3. 1, pefneo, respectively. meanwhile, the epo signal peptide sequence was mutated so as to form a restriction enzyme cut site : bin i. thus the recombinant plasmid can be used as secreting plasmid expressing other gene

    本實驗通過3 』端互補，進行引物延伸合成epo信號肽序列：信號肽和hsblys基因採用重疊延伸拼接法形成融合基因；融合基因分別插入pcdna3 . 0 、 pcdna3 . 1 、 pefneo真核載體：引物延伸合成信號肽時，利用亮氨酸同義密碼，將信號肽基因的倒數第二個密碼突變，在重組載體上的信號肽序列之後，形成bln酶切位點，使三種載體成為分泌表達載體。
  7. Methods : the rearranged gene fragment coding tcr y v region of the jurkat cell line was obtained by rt - pcr technique the pcr product was cloned into the eukaryocytic expressive vector pcdnas to construct pcdna3 / tcr y. after confirmed by sequncing. pcdnas / tcr y plasmids were amplified in bacteria extracted by alkaline lysismethod

    方法：本文採用rt ? ? pcr的方法擴增jurkatt淋巴瘤細胞特異性重排的tcr可變區基因片段，克隆到真表達載體pcdna _ 3中，經序列測定無誤后，堿裂解法大量提取質粒，制備dna疫苗。
  8. In the research of transgenic fish, green fluorescent protein gene was sub - cloned to downstream of carp p - actin gene promoter that was cloned in pucusa by molecular recombination technology. thus pagfp plasmid was constructed successfully. the recombination was determined by digestion of restriction enzyme and sequencing

    實驗通過分子重組技術，採用定向克隆法將綠色熒光蛋白基因亞克隆到puc118a上鯉魚-肌動蛋白基因啟動子下游，構建成能在真核生物體內表達的表達載體pagfp ，經雙酶酶切法序列鑒定后，回收帶啟動子和目的基因片段。
  9. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  10. Abstract : the time - variant reliability analysis of ship hull girders subjected to the deg r adations of fatigue and corrosion is quantitatively carried out in this paper. th e analytical formulations are derived and simple program is compiled. as a case s tudy, the time - variant reliability of a vlcc tank is calculated. it shows that th e degradation effects of fatigue and corrosion are very important for the load - c a pacity calculation of thsip hull girders. after the second special inspection ( 10 years ), the detailed inspection should be done in order to guarantee the safety of ship navigation

    文摘:定量考慮船體構件疲勞裂紋擴展和環境腐蝕對船體梁承載能力的雙重衰減因素的影響，對船體梁的時變可靠性進行了分析，列出了時變可靠性分析公式並編制了相應的計算程序.以一艘大型油船的結構可靠性為例，表明疲勞裂紋和環境腐蝕在第二個特檢年（ 10a ）之後對船體梁承載能力有顯著的影響，此時開展詳細的船體結構檢查對保證船舶營運的安全性具有重要的意義
  11. Forms authentication provider overload list

    重載列表
  12. Lymphotoxin ( lt ) is a kind of pleiotropic lymphocyte - secreted cytokine which mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. in order to increase the antitumor activity of lymphotoxin and reduce its side effects, the recombinant plasmid pet36b - lt 27 was constructed to express soluble fusion protein cbd - lt 27. the active form of lt 27 could be collected directly with several simple steps by three kind of components on the expressed fusion protein

    本研究通過構建表達n端缺失27個氨基酸的淋巴毒素融合蛋白的重組質粒，在大腸桿菌中實現融合蛋白的可溶及分泌表達，同時利用表達載體上的幾種特殊序列經簡單的分離純化步驟直接獲得大量的有生物活性的淋巴毒素缺失體lt 27 ，為尋找一種高抗腫瘤活性、低臨床毒副作用的生物抗癌藥物進行了有效的探索。
  13. This expression vector plbcas - hsa - lgl has the following advantages : i ) the 1. 7kb promoter is able to drive cell - specific and hormone - dependent expression ; ii ) the inclusion of intron - 1 can increase expression level of fusion genes ; iii ) the 5 ' utr of bovine p - casein mrna may have a positive role in both transcriptional and post - transcriptional regulation ; iv ) the gfp gene make the selection of positive clone among embryos possible ; v ) the gfp gene can be easily excised via cre - mediated recombination between the two loxp sites after the expression vector has been integrated into chromosome ; vi ) the two incompatible lox sites, loxp and lox2272, would facilitate cre - recombinase mediated cassette exchange ( rmce ), which in theory will leading to develop a technology of site - specific gene expression in animal mammary glands

    該載體的特點是：具有可以調控外源基因在乳腺中特異表達的牛-酪蛋白基因5 `端側翼區和包括第一外顯子及內含子在內的5 `端調控區；將人血清白蛋白cdna準確地置於牛-酪蛋白基因第二外顯子中的翻譯起始密碼子atg之後，而且沒有增加額外的序列和使人血清白蛋白cdna移碼；引入標記基因gfp ，便於在胚胎期鑒定陽性胚胎，減少受體；引入cre lox重組系統： （ ? ）標記基因gfp的兩端的兩個loxp位點可以在表達載體整合到基因組后，刪除標記基因； （ ? ）餘下的一個loxp位點可以和前面的lox2272位點組成cre重組酶介導的盒式交換系統。
  14. These include cell growth characteristics, expression levels, intracellular and extracellular expression, posttranslational modifications, and biological activity of the protein of interest, as well as regulatory issues in the production of therapeutic proteins. in addition, the selection of a particular expression system requires a cost breakdown in terms of process, design, and other economic considerations. section i : construction of pet22b ( + ) / hpk - 5 vector the hpk - 5 gene encoding 82 amino acid residues from c462 to c543 was recombined with the sequence of plasmid pet22b ( + ) for constructed a new expressed vector pet / hpk - 5

    方法在對hpk - 5 （ humanplasminogenkringle5 ， hpk - 5 ）因子的基因序列和蛋白質序列進行分析的基礎上，利用pcr技術分別構建其可溶性原核表達載體和不溶性原核表達載體；用pcr快速檢測法及其基因測序儀測序以鑒定pet22b hpk - 5和pbv220 hpk - 5重組質粒，用不同的感受態大腸桿菌（ e
  15. They found that np30 had partial cross - reaction with sea and maa and could be used as " antigen reagent " in the immunodiagnostic assays based on antibody detection of schistosomasis japonica. it is difficult to obtian gaa by genetic engineering methods, because gaa is glycoprotein

    基固，擴增產物鑒定后，將其克隆apuc19載體，重組子用san驢r 』 s雙脫氧鏈終止法和自動測序法測定其序列，序列與genebank中及已發表的抗體序列進行比較分析。
  16. The plant expression vector pbin19 - rok219 was constructed, which contains cauliflower mosaic virus ( camv ) 35s promoter and noster. introduction of ibdv vp2 gene under control of camv 35s promoter resulted in the construction of binary expression vector pbr - vp2. then the vp2 gene was in the left and right border regions, which denote the limits of the dna that is integrated into the plant genomic dna via agrobacterium fwme / ac / ms - mediated transformation

    構建了表達載體pbin19 - rok219 ，以ibdv甘肅天水株的抗原基因vp2為目的基因，將vp2基因置於植物組成型表達啟動子camv35s之下，構建了一個ibdvvp2基因的植物表達載體pbr - vp2 ，這樣使vp2基因位於農桿菌t - dna的左右邊界重復序列之間。
  17. These load, speed and mass is reference of choice only

    表中所列基本額定載荷、極限轉速和重量，僅供選擇參考用。
  18. However, removing and reloading modules after initial boot will affect this order

    但是，在開機后移除或重新載入模組將會影響這份列表的順序。
  19. Keyword. not all operators can be overloaded, however, and others have restrictions, as listed in this table

    但不是所有的運算符都可被重載，下表列出了不能被重載的運算符：
  20. As you can see, we have all the bare essentials - a description of gentoo linux, a features list, a daily changelog automatically updated thanks to python, and a bunch of important links to the download sites, to our mailing list sign - up pages, and to cvsweb

    可以看到，我們有了所有最基本的要素gentoo linux的描述、功能部件列表、每日「更改日誌」 （由python自動更新） ，以及許多重要鏈接（鏈接到下載站點、郵件列表注冊頁面和cvsweb ） 。
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