重鏈基因 的英文怎麼說
中文拼音 [zhòngliànjīyīn]
重鏈基因
英文
igh-
By applying the th model theory to analyzing the cases of developed countries that mentioned in chapter 4, chapter 6 finds out the block gene and tendon compages that consists of the university - government - industry th. furthermore, it gives the pheno - types as some external expressions of the genotypes and the family - tree diagram constituted by these phenotypes. after a transition, it illuminates the scientometric, the webometric and the triple helix algorithm and their application
第六章運用th模型理論對第四章提供的案例挖掘梳理后，發現並給出了：構成大學?政府?產業三重螺旋體的完整板塊配基和鍵鏈組合，作為「基因型」外在表達的一些「現象型」屬類以及由它們所構成的圖式譜系。In this study five recombinant plasmids containing hn ( hemagglutinin - neuraminidase ) genes of ndv ( newcastle disease virus ) were constructed, hn genes were amplified by reverse transcriptase - polymerase chain reaction ( rt - pcr ) and were inserted directly into eukaryotic expression plasmid pcdna3
應用反轉錄-聚合酶鏈反應（ rt - pcr ）獲得兩株新城疫病毒（ ndv ）血凝素-神經氨酸酶（ hn ）基因cdna ，然後定向插入真核表達質粒pcdna3中，構建了含hn基因的重組質粒。The modern technology give a chance for the pattern of transmitting knowledge with network, the course based on network have opened up it ' s way in china, our pursuer paid more attention to the mode, but in america, pursuer have studied the more width aspect, thereinto the investigation and design of the information in the course based on network is very important, for the design of the information decide the pattern how the student receive the information, which will effect the way of knowledge understanding and memory, and the way is related to the study pattern, so the design of the information in the course based on network is very important to the efficiency and effect. so the core of the paper is the investigation and design of the information in the course. i have the entropy from the information science as the analyse tool to analyse the information, these work tell us the efficient pattern to transmit information, based on the result, i design the net page, of course, study theory and the character of the net itself are also the factors i employ for design a good net page. then, links and navigation is constituted for students adapt to the course based on network
當今技術的發展給知識以網路為媒介來傳播的學習方式帶來前所未有的機遇，網路課程在國內迅速開展起來，但是國內研究者的注意力主要集中在對網路課程模式的探索上，放眼國外，他們的研究觸角已經涉及到網路課程比較細致的方面，尤其是對網路課程信息的研究構成了國外網路課程研究中的比較重要的一個方面，而網路課程中信息的設計是重要的，因為信息的組織設計是為了接受者能對信息進行有效的信息加工，信息的呈現模式影響著學習者對知識的理解和記憶方式，進而決定了學習者的學習模式，因此網路信息的設計在網路課程傳遞的效率和效果中就佔有很重要的位置。基於這個觀念，本文把網路信息的組織設計作為研究的重點，引入了信息科學作為研究的主要工具對網路中的信息作以量化分析研究，主要應用了信息科學中信息熵的公式進行推導，得到學習內容信息組織的基本模式，並充分利用網路自身特性和學習理論對知識信息進行細致的設計，此外還對鏈接和導航信息進行了設計，在網路課程的適應性方面作出了努力，把交互信息與輔助學習信息的分析設計與應用和對網路頁面信息的總體調節優化作為主體信息設計部分的補充，最終形成了網路信息組織設計的方案，力求創設一個能夠有效傳遞知識信息，減少網路自身弊病，並帶有一定適應性的網路學習環境，也使更多的網路課程的設計者關注網路信息這個因素。Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。Elevation of intracellular calcium ions may be partly induced by increased influx through sarcolemma l type - calcium channels. intracellular calcium elevation, on one hand, would activate calpain, a calcium - dependent cysteine protease that degrade the myofibrillar proteins and cause muscle atrophy ; on the other hand, result in activation of calcineurin which enhance the activity of mhc i promoter and inhibit a shift of mhc isoforms from slow to fast in soleus
這樣，可能使得萎縮比目魚肌細胞內鈣離子水平升高，細胞內鈣離子靜息濃度的增加一方面激活calpain ，增加收縮蛋白的降解，使肌肉萎縮；草四軍醫大月卜祠成士學位論文另一方面通過激活鈣調神經磷酸酶，增加快型mhc基因的表達，使骨骼肌肌球蛋白重鏈（ mhc ）發生由慢型向快型的轉化。To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei, and to study the function and mechanism of mint - mediated transcription repression, yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5, amino acids 2226 - 2959 ). from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo, fifty - one were positive for nutritional screening and p - galactosidase assay. restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments, which are mint, alpha a - crystallin - binding protein i ( alphaa - crybpl ), and nuclear receptor co - repressor 1 ( n - corl ), respectively
鑒于mwt基因編碼區較長，共有10799個堿基，故此我們將mint分為六段，分別命名為fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段為研究對象，將h者分別插入pgb盯7載體中，結果顯示： mintfs可與核受體輔助抑制因子1階cori ） ， o晶狀體蛋白結合蛋白1hlphatcrybp入及mw加互作用，而f6可與igm的重鏈恆定區、泛素結合酶2l6和一個未知功能的新的蛋白基因進行結合。Thus, immunologists have sought smaller molecules with the antigen - recognition capability of antibodies. the variable domains of the heavy ( h ) and light ( l ) chains are sufficient for antigen recognition but the non - covalent complex of the two variable domains ( fv ) is unstable. it is possible to genetically engineer a single - chain fv ( scfv ) with the h chain v region connected to the l chain v region via a 15 amino acid linker composed of serine and glycine amino acid residues
二、日本血吸蟲單克隆杭獨特型抗體np30的單鏈狐（ scf ）的構建、表達及對balbic小鼠誘導保護性作用研究l 、日本血吸蟲單克隆杭獨特型抗體np0的單鏈抗體歸cfv ）的構建、表達通過pcr方法體外擴增並經測序驗證的重鏈、輕鏈可變區（ vh 、 vl ）基因先後重組入原核表達質粒ptha90相應的位點上，中間通過一連接肽（ gly在er ） 。Recovery estimated from the safh4 plant line indicates that 9 ( jl g of pure active scfv can be obtained per gram of fresh leaf material, on a laboratory scale. the production of the scfv antibody proteins in plant root exudates was also addressed. the scfv antibody protein was continuously secreted from the transgenic tobacco roots into a simple hydroponic medium at 630 to 760 ng g - 1 dry weight of root day - 1
在水培條件下，轉基因煙草根可連續分泌具有活性的重組抗乙肝病毒表面抗原pres1 （ 20 - 47 ）單鏈抗體進入到液體培養液中，不須破壞植物即可連續獲得重組單鏈抗體，為利用植物生物反應器連續生產單鏈抗體開辟了新途徑。The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size. computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70. 3 % that would encode a protein of 552 amino acids ( aa ). the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp
對phz1754進行外切核酸酶（ exonuclease ， exo ）順序缺失，獲得單向長度漸減重疊的系列突變體，核苷酸序列測定顯示出該ecor - sal片段的精確大小為2299bps ， frameplot程序分析揭示出該區域一個完整的開放閱讀框（ orf ）的存在，其大小為1656bps ， g + c含量為70 . 3 ，編碼552個氨基酸，利用blastsearch程序將orf的核苷酸序列及推導的氨基酸序列與因特網上基因及蛋白質數據庫進行綜合比較，發現無論在核苷酸水平還是在蛋白水平上，該orf均與膽固醇氧化酶表現出同源性，而且與鏈黴菌膽固醇氧化酶同源性最高，說明該orf編碼膽固醇氧化酶基因。A 40 - base polymorphic repeat sequence located in the 3 ' - untranslated region of the dat gene was purified and amplified by polymerase chain reaction ( pcr )
將位於多巴胺運輸器基因3 '端未轉譯區段的40 -堿基多形性重復序列予以純化、經聚合酶鏈鎖反應放大。Based on a 3. 1kb pst i fragment of genomic dna of a wild s. avermitilis, a 1. 5 kb apramycin resistance fragment was inserted into sph i site of avec gene in the 3. 1 kb fragment, then a recombinant plasmid pc05 was obtained by introducing above inactivated avec fragment into mcs region of phjl401. competent cells of et12567 were transformed by recombinant plasmid pid03 and pc05 respectively
以含有avec基因的3 . 1kb基因組dnapsti片段為基礎，將1 . 5kb的安普黴素抗性基因片段插入到avec基因中的sphi酶切位點，再將此插入失活的avec基因片段連接到具有接合轉移功能（含有orit基因）的鏈黴菌-大腸桿菌穿梭質粒phjl401的多克隆位點區，由此得到重組質粒pc05 。As well as in eukaryocyte ( hepg2 and cos - 7 ), then detect their antigenity as a basis study and explore of the choice of immunogen for preventive and therapeutic vaccines of hepatitis b. methods : the gene fragments coding 152aa ( si ) and 124aa ( s2 ) of the carboxyl terminus of hbsag were amplified by pcr from plasmid pecob6 with a pair of primers containing different endonuclease sites and were cloned into multiple cloning sites of plasmid pbks ( + )
為乙型肝炎的預防和治療性疫苗免疫原的選擇進行初步的研究和探討。方法：本研究利用聚合酶鏈反應（ pcr ） ，通過設計帶有不同酶切位點的一對引物，從質粒pecob6特異性擴增hbsag蛋白羧基末端152個氨基酸（ s1 ）和124個氨基酸（ s2 ）的基因片段，分別將二者克隆到質粒pbks （ + ）的多克隆位點，篩選重組克隆。Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells, we design a pair of particular primers, and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic. the expression plasmid was identified with pcr and dna sequencing. pbv220 - r hpf4 was transformed into e. coli dh5a, bl21 ( de3 ) and induced by increasing the temperature to 42. we identified the expression protein by sds - page and western - blotting
目的1人血小板因子4 （ hpf4 ）原核高效表達克隆的構建2重組hpf4的表達及分離、純化工藝研究3重組hpf4的特性研究方法根據原核細胞表達真核蛋白的基因表達調控特點，設計合成一對特異引物，在pt7 - 7 - rpf4表達質粒的基礎上，應用聚合酶鏈式反應（ pcr ）對其cdna進行改造，通過dna重組技術構建成重組hpf4原核表達質粒pbv220 - rhpf4 ，用快速pcr檢測法、 dna測序分析，鑒定重組hpf4表達質粒的正確性。They inhibits the growth of fungi ( filamentous fungi especially ) while are non - toxic to plant cells. the main results were as follows : 1. obtaining of spcema ( signal peptide modified cema ) spcema ( 187bp ) was amplified with two long complementary primers ( p2 and p3 ) and two primers ( pl and p4 ) containing restriction enzyme recognition site
帶信號肽cema基因的pcr合成以兩條部分重疊長鏈引物p _ 2和p _ 3延伸產物為模板， p _ 1和p _ 4為正、反引物進行pcr擴增，獲得了改造的抗菌肽基因spcema （ 187bp ） 。Application of rapid detection for rifampin resistance in clinical isolates of mycobacterium tuberculosis by phage amplified biologically assay
等位基因特異性多重聚合酶鏈反應方法用於快速檢測結核分枝桿菌利福平耐藥株This is important in attempting to use antibodies as anti - tumor reagents, for example
基因為日本血吸蟲單克隆抗獨特型抗體np30輕鏈和重鏈可變區基固。The complicated life cycle of streptomyccs is one of the most important reason that attracts researchers on its genetics. a great deal of valuable information on the morphological, physiological differentiation of streptomyccs coclicolor will emerge after genome sequencing is completed. based on the s. coelicolor dna sequences that have been finished and focusing on three ftsk homologous, which may be closely related with cell division, this study tried to find out the functions of three ftsk homologues genes by gene replacement or disruption
鏈黴菌復雜的生活史是研究其遺傳學的一個重要原因，天藍色鏈黴菌全基因組測序將會為其形態及生理分化發育的研究提供大量有用的信息，本研究基於天藍色鏈黴菌已測出的98序列，對一組可能與細胞分裂有關的基因? ftdk同源基因? ?進行基因置換或中斷並對其功能進行初步研究。The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library. one of positive scfv clones, named pcsal, was selected with phage - elisa after panning and screening by bull sperm three times. scfv fragment, amplified from pcsa1, was ligated to pmd18 - t vector for sequencing analysis
取陽性重組噬菌體抗體克隆株pcsa1 ， pcr擴增其scfv基因，篩選重組子進行序列測定，發現其序列符合小鼠抗體基因的一般特徵，並且與幾株抗磷酸膽堿的抗體重鏈和輕鏈可變區序列的同源性達80以上；推測pcsa1scfv針對的抗原是磷酸膽堿類物質。In the present study, the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis. total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies. after obtained using rpas system, vh and vl genes were used to assemble scfv gene fragment with a linker primer
應用重組噬菌體抗體庫技術，從分泌小鼠抗牛精子sp18抗體的雜交瘤細胞系中分離總rna ，克隆抗體重鏈和輕鏈可變區基因，加入連接肽引物（ linkerprimer ）組裝成單鏈抗體scfv （ singlechainfragmentvariable ）基因並用rs引物進行擴增， sfi 、 not酶切，回收后與pcantab5e載體相連，轉化e . colitg1宿主菌，構建單鏈抗體文庫。It was also proved that the biosynthestic genes of apramycin was linked to the apramycin resistant gene in s. tenebrarius
Pcr實驗證明基因重組菌株中接合轉移質粒同源整合到黑暗鏈黴菌h6的染色體上。分享友人