鏈增長反應 的英文怎麼說
中文拼音 [liànzēngzhǎngfǎnyīng]
鏈增長反應
英文
chain extending reaction- 鏈 : Ⅰ名詞1. (鏈子) chain Ⅱ動詞(用鏈栓住) chain; enchain Ⅲ量詞(計量海洋上距離的長度單位) cable length
- 長 : 長Ⅰ形容詞1 (年紀較大) older; elder; senior 2 (排行最大) eldest; oldest Ⅱ名詞(領導人) chief;...
- 反 : Ⅰ名詞1 (方向相背) reverse side 2 (造反) rebellion 3 (指反革命、反動派) counterrevolutionari...
- 應 : 應動詞1 (回答) answer; respond to; echo 2 (滿足要求) comply with; grant 3 (順應; 適應) suit...
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Polymers are most commonly formed by two main types of growth reaction traditionally referred to us condensation (step reaction) and addition (chain reaction) processes.
形成聚合物的最普通方式是靠兩種主要的增長反應,習慣上分別稱做縮合(逐步反應)過程和加成(鏈式反應)過程。It is the reverse of the propagation step in addition polymerization.
這一過程是加聚反應歷程中鏈增長步序的逆過程。Chain propagation reaction
鏈增長反應After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence
F _ 1長38bp , r _ 1長36bp ,其它片段均40bp長, f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應,然後用其他單鏈片段作為引物,進行pcr擴增,用dna快速純化回收試劑盒回收所得254bppcr產物,與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞,利用藍白斑遺傳學篩選法篩選陽性克隆,提取其質粒,採用nco和xho雙酶切鑒定,獲得了254bp的片段;用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定,獲得300bp的片段。Although document business is expected to decline as paper data can be transmitted via internet applications, overseas buyers tend to trim their inventory requirements by just - in - time supply chain management. thus, the demand for express services for handling last minute order, prototypes, samples and small packages would sustain the growth
不過,海外買家越來越多採用及時反應的供應鏈管理,減少存貨,因此對處理緊急訂單、原型、樣辦及小批量貨物的速遞需求將推動業界的增長。In this study, the whole e2 genes of two strains of classical swine fever virus ( csfv ) isolated from guangxi ( gx ) were amplified by reverse transcriptase polymerse chain reaction ( rt - pcr ) method and seqenced. the e2 gene fragments of csfv were 1090 base pair in length and encoded 364 amino acid residues
研究採用反轉錄?聚合酶鏈式反應( rt ? pcr )技術對兩株分別從柳州( gxlz )和南寧( gxnn )分離的廣西流行豬瘟病毒( classicalswinefevervirus , csfv )進行e _ 2全基因的擴增、克隆和測序。擴增片段長度為1090bp ,編碼364個氨基酸殘基。Finally, these departments ca n ' t establish the information system and logistic system which can quick respond to the customers relate to cost. quality deliverable our country economy system is changing, it is changing from socialism planning economy to market economy. it is very important for us to research the supply chain management, we can learn from the foreign country " s enterprise practice. our goal of use of scm is controlling inventory, improving supply quality and strengthening the competitive capability
我國航空企業特別是材料采購在傳統計劃體制與運作模式下,多數是自成一個封閉系統,不重視對供應鏈的管理;製造商、供應商和經銷商缺乏長期合作的戰略夥伴關系,相互之間缺乏以信任和共同獲利為基礎的價值鏈,沒有形成滿足顧客在成本、質量、交貨時間等方面的要求而反映敏捷的物流和信息流。我國正在由計劃經濟體制向市場經濟體制轉變,研究國外近來發展迅速的供應鏈管理理論與方法並應用於gj航空公司的實際,對控制庫存、提高服務水平、增強市場競爭能力,有重要的現實意義。分享友人