鏈絲菌素 的英文怎麼說
中文拼音 [liànsījūnsù]
鏈絲菌素
英文
streptothricin-
Fr - 008 is a strain producing a complex of heptaene macrolide antifungal antibiotics, one of which, previously named fr - 008 was regarded to bis similar to candicidin, but different in sugar moiety
Hplc分析研究表明，它與灰色鏈黴菌imru3570產生的殺假絲菌素是同一種物質，均含有相同的幾種主要組分。First, after investigation of two original strains " biological characteristics, we studied the main influence factors on protoplasts formation and regeneration in s. mycarofaciens and s. erythreus, and determined the best protoplasts formation and regeneration conditions of two original strains. the former shake - cultured in s " medium at 28 ?, 220r. min ~ ( - 1 ) for 24h, lysised by 3mg / ml lysozyme, keeping warm at 32 ? for 50 ~ 60min, regenerated on r _ ( 5 " ) medium, 28 ? for 5 ~ 6d. the latter used two - step culture, then used img / ml lysozyme keeping warm at 37 ? for ih ; the protoplasts were plated on r5 " regeneration medium at 28 ? for 5d
首先在對兩親株的生物學特性進行了鑒定后，考察了影響兩親株原生質體形成和再生的主要因素，確定了生米卡鏈黴菌和紅黴素鏈黴菌原生質體形成及再生的最佳條件：前者用s培養基，在28 、 220r . min ~ （ - 1 ）培養24h后，用3mg ml的溶菌酶在32恆溫酶解50 60min ，得到的原生質體在乾燥的r5培養基上28倒置培養5 6天，可得到再生率在20左右的再生菌落；後者採用二級菌絲培養，用1mg ml的溶菌酶在37恆溫酶解1h左右，得到的原生質體也在乾燥的r5培養基上28倒置培養5天，即可得到再生率在20左右的再生菌。16 replacement plasmids for two different regions were obtained, but have n ' t been introduced into 10 - 22 successfully. 42 cosmids in this region were introduced into a heterogeneous host zx64, one transformant exhibited a very faint inhibit activity against fusarium oxysporum when growed on a medium with thiostrepton
將300kb區域的柯斯質粒進行異源表達，發現有一文庫質粒5h4的轉化子在硫鏈絲菌素存在時表現出對棉花枯萎病菌微弱的抑制活性；無硫鏈絲菌素存在時，則檢測不到。
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