鏈黑黴素 的英文怎麼說
中文拼音 [liànhēiméisù]
鏈黑黴素
英文
bruneomycin-
Arginine feeding experiment showed that nitrogen metabolism in the s. tenebraius was obviously affected by arginine through two possible ways : ( l ) pronase activity in vitro could be influnced by arginine, as a result, the catabolism of nitrogen - containing macro - molecule was promoted and the nitrogen element in the broth was increased. ( 2 ) arginine could be transformed into glutamic acid, so that the biosynthesis of apramycin was promoted
因而我們認為gln可能是安普黴素生物合成氮元素的供體。 arg添加實驗結果表明， arg可能通過兩種途徑影響黑暗鏈黴菌體內的氮代謝： （ 1 ） arg可能影響胞外蛋白酶的活性，進而促進含氮大分子物質的分解代謝，補充發酵過程中的氮素來源。S. lividans mutant strains zx1 ( dnd cluster deleted ) and zx64 ( dnda disrupted ) had pleiotropic mutations including low mel expression and poor sporulation. it was speculated that dnda together with its downstream dna ( 2. 5kb ) might be involved in these two phenotypes because dnda together with its downstream dna could restore normal sporulation and mel expression to zx64, while dndb and dndc had no such effect because lai and la2 showed no obvious difference in these two phenotypes from wild type s. lividans 1326
另外通過比較這幾個突變株及野生型菌株在產孢和黑色素基因（ mel ）表達方面的差異，推測dnda及其下游區域與變鉛青鏈黴菌的產孢和刺激外源黑色素基因的表達有關，而dndb和dndc則與之無關，因為la1和la2在這兩種表型上與野生型菌株無明顯差異。To study the biosynthetics genes of apramycin in streptomyces tenebrarius, the apramycin resistant gene was isolated by gunshot cloning firstly
為了研究黑暗鏈黴菌安普黴素生物合成基因，首先通過鳥槍克隆的方法克隆其安普黴素抗性基因。Plasmid psyxl, containing a 12kb insert of s. tenebrarius dna, was isolated from one of six colonies. apramycin resistant gene was located in 1. 5 - kb sphl - kpnl fragment of plasmid psyxl though further studies
陽性克隆中重組質粒psyx1含有12kb黑暗鏈黴菌的dna ，對12kb片段進一步分析將安普黴素抗性基因定位在1 . 5kbsphi - kpni片段上。分享友人