鑒別病原體 的英文怎麼說
中文拼音 [jiànbiébìngyuántǐ]
鑒別病原體
英文
identify the pathogen- 鑒 : Ⅰ名詞1 (鏡子 古代用銅製成) ancient bronze mirror2 (可以作為警戒或引為教訓的事) warning; objec...
- 別 : 別動詞[方言] (改變) change (sb. 's opinion)
- 病 : Ⅰ名詞1 (疾病; 失去健康的狀態) illness; sickness; disease; malum; nosema; malady; morbus; vitium...
- 原 : Ⅰ形容詞1 (最初的; 原來的) primary; original; former 2 (沒有加工的) unprocessed; raw Ⅱ動詞(原...
- 體 : 體構詞成分。
- 病原體 : agent of disease
- 病原 : (病因) etiology; aetiology aitiology; noxa (pl noxae); cause of disease; pathogeny病原蟲 prot...
-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。Since pneumonia is associated with inflammatory features in the sputum but no gram ' s staining of microorganisms, the differential diagnosis should focus on a iral, chlamydial, or mycoplasmal infection, although this patient ' s prior exposure to antibiotics may reduce the likelihood of finding another pathogen on gram ' s staining
既然肺炎病人的痰液應伴隨炎癥特徵,而該患者的痰液革蘭氏染色檢查未見微生物,故鑒別診斷應該集中在病毒、衣原體或支原體感染,雖然患者先前的抗生素治療可能會降低革蘭氏染色檢查中其他病原體的發現率。Be based on this one principle, tuberculin is used extensively at infection of the tuberculosis epidemiology investigation, n / med tuberculosis that monitor, tuberculosis differentiate diagnose, object of have an inoculation of choice bcg vaccine and effect of assessment have an inoculation, still can use at deciding the cellular immunity ability of human body
基於這一原理,結核菌素被廣泛用於結核病流行病學調查、監測結核感染、結核病的鑒別診斷、選擇卡介苗接種對象及考核接種效果,還可以用於判定人體的細胞免疫能力。Abstract : we have investigated the ultrastructural changes of the biopsied muscle specimens from 8 cases of metabolic myopathies including 3 cases of glycogenosis, 2 cases of lipid storage myopathy and 3 cases of mitochondria myopathy. diagnosis and differential diagnosis with tem for metabolic myopathy as well as the significance of some ultrastructural changes were discussed in this study
文摘:本文對3例糖原累積病, 2例脂質沉積性肌病和3例線粒體肌病共8例代謝性肌病肌活檢標本的超微病理改變進行了觀察分析,對代謝性肌病的超微病理診斷、鑒別診斷以及某些病理改變的診斷意義進行了初步探討。The act or process of identifying or determining the nature and cause of a disease or injury through evaluation of patient history, examination, and review of laboratory data
診斷通過評價病人的病史、體檢情況和閱讀試驗數據的方式對人體機能進行檢驗或對人體引起病痛的原因進行鑒別的行為或過程The main works of this paper are presented as follows : ( 1 ) analyzed the research condition and the difficulty of multi - agent security research at present, and we gave the definition of security in a multi - agent system ; ( 2 ) analyzed and sampled involved immune mechanism, we constructed a immune agent and described its mental attribute by dfl ; ( 3 ) gave a communication form based on mental attribute ; ( 4 ) based on dfl, we proposed a diseriminating model among tias by taking into account the theories of dynamic immune network ; ( 5 ) based on the principle of clone selection, we gave a tia ’ s immune algorithm, and put forward single tia ’ s distinghuishing model and multi - tia ’ s distinghuishing model by taking this algorithm as foundation ; ( 6 ) the security model carried on an application in the detection of computer virus, for this purpose, we also developed a simple simulation program based on binary coding
主要包括如下工作: ( 1 )分析了當前多agent安全的研究現狀及其面臨的困難,給出了多agent安全的需求和定義; ( 2 )分析和抽取了相關的免疫機制,構造了一種免疫agent ,並利用動態模糊邏輯對其非空心智狀態進行了描述; ( 3 )給出了agent之間基於心智屬性的通信方式; ( 4 )以動態免疫網路理論為基礎,結合動態模糊邏輯,給出了tia的狀態鑒別模型; ( 5 )給出了基於克隆選擇原理的tia免疫演算法,並在該演算法的基礎上提出了單tia非自體識別模型和多tia協商非自體識別模型; ( 6 )將該安全模式在計算機病毒檢測中進行了應用,實現了一個簡單的基於二進制編碼的計算機病毒檢測模擬系統。分享友人