鑒定細菌學 的英文怎麼說

中文拼音 [jiàndìngxìjūnxué]
鑒定細菌學 英文
dairy bactedology
  • 鑒 : Ⅰ名詞1 （鏡子 古代用銅製成） ancient bronze mirror2 （可以作為警戒或引為教訓的事） warning; objec...
  • 定 : Ⅰ形容詞1 （平靜; 穩定） calm; stable 2 （已經確定的; 不改變的） fixed; settled; established Ⅱ動詞...
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 學 : Ⅰ動詞1 （學習） study; learn 2 （模仿） imitate; mimic Ⅱ名詞1 （學問） learning; knowledge 2 （學...
  • 鑒定 : 1 （評語） appraisal （of a person s strong and weak points） 2 （評定） appraise; identify; auth...
  • 細菌 : germ; bacterium （pl. bacteria）; fungus （pl. fungi）
  1. After further purifing the isoloated bacteria, we classified and characterized them, according to their individual form, physiological and biochemical to characterize, characterize to bacteria genus. calculated the total of aerobic bacteria in soil of each senson, the total of bacteria of degrading omethoate, as well as the percentage of each degradation bacteria genus, and measured the physico chemical nature of each seasonal soil sample

    將分離到的菌株進一步純化后，按其個體形態、生理生化特徵，進行分類鑒定，鑒定到菌屬；計算出各季節土壤中的細菌總數、降解有機磷農藥氧樂果的細菌總數以及各降解菌屬的百分比，測出各季節土壤樣品的物理化學性質。
  2. Be based on this one principle, tuberculin is used extensively at infection of the tuberculosis epidemiology investigation, n / med tuberculosis that monitor, tuberculosis differentiate diagnose, object of have an inoculation of choice bcg vaccine and effect of assessment have an inoculation, still can use at deciding the cellular immunity ability of human body

    基於這一原理，結核菌素被廣泛用於結核病流行病學調查、監測結核感染、結核病的鑒別診斷、選擇卡介苗接種對象及考核接種效果，還可以用於判定人體的細胞免疫能力。
  3. After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence

    F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。
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