間酶 的英文怎麼說

As the kid chymosin was extracted by the traditional way and the buffering way at different ph values, its activity mainly depended on the salt concentration, extraction time and temperature, the ratio of buffer and abomasums and extraction times

用傳統方法和不同ph緩沖液方法提取羔羊凝乳酶時，食鹽濃度、提取時間、提取溫度、提取液與皺胃比例、提取次數對凝乳活性有重要的影響。

The different locus outcrossing rate of adiantum reniforme l. var sinese y. x. lin has great change in the same population, and the outcrossing rate difference of the same enzyme locus is also high in the different population

荷葉鐵線蕨不同位點的異交率在同一居群內的變化很大，而不同居群間的同一酶位點的異交率差異也是較大的。

Transketolase is the key enzyme of pentose - phophate pathway, catalyses transfer of a two - carbon fragment from a ketolase ( donor substance ) to an aldose ( acceptor substance )

摘要轉酮醇酶是磷酸戊糖途徑的關鍵酶，催化二碳單元在酮糖（供體）和醛糖（受體）間的轉移。

In addition to avermectins, s. avermitilis produces oligomycin, a strongly toxic compound. gene deletion vector pxl05 was used to disrupt oligomycin polyketide synthase ( pks ) encoding genes ( olma ) in streptomyces avermitilis cz8 - 73, the producer of anthelmintic avermectins b and the cell growth inhibitor oligomycin. olma gene cluster in the chromosome was displaced by deletion allele on the plasmid via double crossover

本研究以產阿維菌素b和寡黴素的阿維鏈黴菌cz8 - 73為出發菌株，構建了基因缺失載體pxl05 ，並將其轉入cz8 - 73中，通過缺失載體和染色體之間的同源雙交換，對染色體上長達90kb的寡黴素聚酮合酶（ pks ）基因簇（ olma ）進行了缺失。

2. the antiserum of rabbit anti - ucp1 from brown adipose tissue of tree shrew was prepared successfully

2 、我們酶聯免疫實驗結果表明ucp1濃度與冷馴化時間呈正相關。

The results showed that the nearer the relationship of the species, the more similar the zymograms. the zymograms difference between species in different genes is more obvious than ones in the same one. in addition, there is a certain difference between the zymograms of different strains of the same species from different ascocarps and places

結果表明，親緣關系愈近的菌株其酶譜的相似性愈大，屬間差異明顯大於種間差異，同菌種的酶譜可因寄主、產地不同等而存一定差異。

A rabbit was infected with a cloned yntatl, blood was collecting from from the rabbit every 3 days after infection within 30 days, 10 clonal trypanosome populations were gotten, infecting a new rabbit by the last non - cloned trypanosome population. repeated above all, thus infected 5 rabbits sequentially. twenty different vats ( variant antigen type ) were monitored and characterized from those fifty mono - clonal populations by indirect immunofluorescence test ( ift ) and avidin biotin enzyme immunoassay ( abc - eia )

用伊氏錐蟲雲南水牛單克隆株yntat1感染兔，感染后30天內，每3天從兔血中分離錐蟲並單蟲克隆，最後一個未單蟲克隆的蟲株感染另一隻兔，重復以上操作，這樣順序感染5隻兔子，共獲得50個單克隆錐蟲種群（ tp ） ，經間接免疫熒光和abc酶標試驗鑒定共為20個抗原性互不相同的抗原變異體（ vats ） 。

One of the first series of controlled laboratory studies providing translational evidence for a molecular reason to maintain high levels of daily low - intensity and intermittent activity came from examinations of the cellular regulation of skeletal muscle lipoprotein lipase ( a protein important for controlling plasma triglyceride catabolism, hdl - c, and other metabolic risk factors )

最初的一系列對照性實驗室研究提供了一些來自於骨骼肌脂蛋白脂肪酶翻譯水平的證據，從分子機制解釋了每日低強度和間歇性運動的細胞調節機制。

In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv

理化學研究表明，該病毒為rna病毒，對氯仿、乙醚敏感；胰酶試驗中，經37 、 1小時處理的病毒，仍然能夠在貓源細胞fcwf細胞上生長，並且毒力基本保持不變；耐酸性試驗中，病毒分別在ph5 . 0和ph3 . 0經37作用2小時，毒力僅下降一個滴度；耐熱性試驗中，該病毒在恆定溫度50 ，設定不同時間，從5分鐘到150分鐘，毒力均有不同程度下降，其中， 50作用30分鐘，病毒平均滴度下降2個單位； 50 ， 60分鐘， cpe消失；恆定時間1小時，設定不同溫度（ 50 - 60 - 70 - 80 ） ，病毒在細胞上完全喪失增殖能力， cpe消失。生物學試驗，利用實驗室現有條件，選擇不同的細胞系對該病毒進行培養，發現該病毒對貓源細胞fcwf最敏感； mdck細胞次之； f81細胞經多次傳代，亦可出現cpe ；而vero細胞則不敏感。血凝試驗表明，該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。

Chlamys farreri, which belongs to mollusca, bivalvia, pterioidae pectinidae, are widely distributed on the china from donghai sea to bohai sea, korea and japan. this species has been the main aquacultrue shellfish for many years in northchina

利用同工酶技術，對中國櫛孔扇貝和日本櫛孔扇貝的遺傳差異進行了比較分析，並對它們的正反交後代的酶表型及遺傳型進行了分析，探討了亞種間雜交的機制。

Compared to control, retention times of digesta in whole alimentary tract of immunized animals inc reased by 20 hours ( to use cumlative excretion of 5 % marker as reference ). immunoneutralization of ss significantly augmented activities of digestive enzymes ( proteolytic, trypsin, chymotrypsin, amylase ) in pancreas and the small intestine ( control and immunized animals were 1693. 67unit / g, cp, 2728. 33 unit / g, cp, 3055. 50 unit / g, cp, 12. 9x106 unit / g, cp ; 2. 57x 102unit / g, cp, 1. 20x103unit / g, cp, 1. 12x 103unit / g, cp, 2. 98x 107unit / g, cp ft 2451. 33 unit / g, cp, 2904. 17 unit / g, cp, 4279. 33 unit / g, cp, 20. 61 x 106 unit / g, cp ; 6. 45 x 102unit / g, cp, 2. 53 x 103unit / g, cp, 1 - 83 x 103unit / g, cp, 5. 77 x 107unit / g, cp, respectively, p < 0. 05 or p < 0. 01 )

12ng ml ， 0人su vg ，各指標比較均差異不顯著， p 0刀5人兔疫組動物的食糜消化道滯留時間明顯增加（以指示劑累計排出50為標準，兔疫組較對照組大約增加20小時） ，與此同時， ss免疫中和也提高了胰腺和消化道各種消化酶的比活力（對照組和免疫組胰腺，小腸食糜總蛋白酶，胰蛋白酶， 」糜蛋白酶和澱粉酶比活分別為1693石7unit g ， cp ， 2728

The complex formed by cnbr - activated alginate and antibody is aggregated to the surface of the paraffin - graphite - chitosan electrode by electrostatic adsorption ( coacervation ). the concentration of sjag can be detected by determining the redox current of o - aminophenol, which oxidized by h2o2 in the presence of hrp. moreover, the immunosensor shows some improved performances including high sensitivity, selectivity and less non - specific adsorption

褐藻酸鈉?抗體復合物通過靜電吸附作用被凝集到含石墨?石蠟?殼聚糖組分的電極表面，然後與抗原和酶標抗原進行競爭反應，以鄰氨基酚為電子媒介，通過測定酶催化下雙氧水對其氧化的電流大小來間接測定抗原的濃度。

With 24 c or 4 c, the change trends of the content of the salidroside was basically consentaneous in the consecutive cultural eras of the callus. in the callus from the different explants, the influence was maximal to the the activity of pal enzyme and the influence was lowest to the the activity of ca4h enzyme, and the influence to the the activity of tal enzyme was ascertained according to the explant of the callus. so in the same explant with the different temperature or in the different explant with the same temperature, there was no incident between the content of the salidroside and the activity of enzymes pal, ca4h and tal, and we presumed that there may be emphasized particularly on different metabolic pathway of salidroside

（ 2 ） 、愈傷組織的外植體來源、培養溫度條件和不同的繼代培養數都影響著其中紅景天甙的含量和苯丙氨酸解氨酶（ pal ） 、肉桂酸解氨酶（ ca4h ）和酪氨酸解氨酶（ tal ）這3種酶的活性；不管是葉來源還是莖來源，不管是24培養還是4培養的愈傷組織，在連續繼代培養中紅景天甙含量的變化趨勢基本上是一致的；無論是葉來源的愈傷組織還是莖來源的愈傷組織，培養溫度對pal酶的酶活性影響最大，對ca4h酶的酶活性影響最小，對tal酶的酶活性影響視不同的外植體來源而定；在相同外植體來源的愈傷組織中及不同的培養溫度的條件下，或是在不同外植體來源的愈傷組織中及相同溫度的培養條件下，其紅景天甙含量與pal酶、 ca4h酶和tal酶的酶活性之間沒有完全一致的對應伴隨關系。

Studies on the activities of enzymes of protective system during diapause of sawfly chinolyda flagellicornis

滯育期間鞭角華扁葉蜂保護酶系統活力

Because of the cleavage site of enterokinase and cnbr was designed in the middle of thioredoxin and cmiv, the expressed peptides of the mutation of cmiv could be cuted down from the fusion protein by enterokinase or cnbr

由於硫氧還蛋白和抗菌肽之間設計了腸激酶（ enterokinase ）切割位點和cnbr切割位點，通過對該表達的融合蛋白的切割，可得到目標抗菌肽cmiv突變體多肽分子。

Enzymatic assay were performed using recombinant proteins expressed in e. coli cells. tilapia 17 - hsd1 is nadp ( h ) dependent and it can catalyze the interconversion from estrone to estradiol efficiently and also from androstendion to testosterone but less efficiently

酶活性實驗結果顯示，在e . coli細胞中表達的17p一hsdi / petbluez重組蛋白既能高效地催化a和t之間的相互轉化，同樣又能更高效地催化e1和ez之間的相互轉化。

In this research, the plant esterase of wheat and - ethanoic acid naphthalene ester were taken as materials to inspect the relation between the wheat esterase and the organic phosphorus, ten linearity equations were established which expressed the esterase vigor and the organic phosphorus density logarithm with the change of the numerical value of ultraviolet resorption, and the monotony range and the extremum of the function were confirmed

摘要本研究以小麥為植物酯酶的酶源， -乙酸萘酯為底物，考察了小麥酯酶與有機磷農藥之間作用的關系，建立了10個以吸光值變化表示的酶活性與有機磷農藥濃度對數之間的線性方程，並確定了函數的單調區間和極值。

The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。

The effect of some factors, including the appropriate materials for isolating protoplasts, the concentrations of enzyme, period of digestion and temperatures, and osmotic pressure stabilizers on the isolation and regeneration of protoplasts in penicillium digitatum were studied. the results demonstrated that the purified protoplasts could regenerate through double layers of czapek medium containing 0. 7mol / l nacl. the regeneration rate could reach 24. 9 %

通過對制備材料、酶液濃度、酶解時間、酶解溫度、滲透壓穩定劑的種類和濃度等因素的實驗研究，得到了一套制備指狀青黴（ penicilliumdigitatum ）原生質體的有效方法，並在雙層培養基上初步實現了原生質體的再生，再生率可達24 . 9 。

Differences of zymograms between families were more significant than those between genera. although zymograms within genera were rather similar, all the species could be unequivocally distinguished from each other. the feasibility about using isoenzymes in systematics was also discussed

蝽類昆蟲科之間酶譜差異大於各屬間的差異；各屬間的酶譜也有顯著差異；而屬內各種間的酶譜比較接近，但每種都有自己的特徵酶譜，彼此易於區別。