閱讀框 的英文怎麼說

中文拼音 [yuèdòukuàng]
閱讀框 英文
orf
  • 閱 : 動詞1. （看） read; go over 2. （檢閱） review; inspect 3. （經歷; 經過） experience; pass through
  • 讀 : 讀名詞（語句中的停頓） a slight pause in reading
  • 框 : 框名詞（框架; 框子） frame; case
  • 閱讀 : read
  1. The pcr product was inserted into expression plasmid pet - 32a ( + ) after restriction digest. then the recombinant plasmid was identified by endonuclease analysis, pcr ampliation and dna sequencing. the report showed that the recombinant plasmid had right open reading frame

    重組質粒經酶切鑒定， pcr鑒定和測序，結果證實豬肺炎支原體黏附因子p97基因的抗原決定簇r1區定向插入了質粒pet - 32a （ + ） ，且閱讀框架正確。
  2. The open reading frame of tsarg2 was obtained from human testis cdna library by pcr. using in situ hybridization on tissue section of human testis. we preliminarily studied the expression and function of tsarg2

    從小鼠睪丸cdv文庫中分離出該基因完整閱讀框cdna ， ； hu基因的cdna全長為1088hp ，包含6個外顯于，基因組跨越9
  3. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  4. Two positive clones were sequenced, and the results showed that its nuclcotidc sequence includes an open reading segment which codes for a 45 - amino acids protein and three endonuclcase sites which arc1 bgii, bamh i and bgi ii, this protein was identified as metallothionein based on its characteristic described above and its similarity ( 85 % ) to the mtn gene of drosophila : the 10 cysteine residues present occur in five pairs of cys - x - cys, x is serine, valine, ilistidine or lysine

    結果顯示：擴增的cdna片段長度為289bp ，其中含有一個編碼45個氨基酸的開放閱讀框，閱讀框所編碼的氨基酸中含有10個半胱氨酸，且在序列中均排列成cys - x - cys ，其中x為ser 、 val 、 his或lys 。這些特徵說明擴增的基因片段為家蠅mt基因序列的一部分。此基因序列片段與果蠅mtn基因序列的同源性達到85 . 0 ，擴增的基因序列中含有三個內切酶位點bg 、 bam和bg ，這一點也和果蠅mtn基因十分相似。
  5. The start reading framae and stop codons, base composition in protein - coding genes and the codon usage of amino acids in scolopendra multilane were compared with the three other myriapods

    本研究在蛋白質編碼基因起始閱讀框和終止密碼子、蛋白質編碼區的堿基組中文摘要成、氨基酸及密碼子的利用等方面把少棘蜈蚣與另三種多足類進行了比較。
  6. We conclude that the gene we studied is a new loci required for efficient nitrogen fixation and for competitive nodulation of soybeans by bradyrhizobium japonicum strain gx201

    我們認為在突變體菌株gx217中tn5gusa5所插入的開放閱讀框架是一個新的與競爭結瘤有關的基因。
  7. Orf, open reading frame

    和開放閱讀框架
  8. It will provide us to further study the function of xanthophyll cycle in photoprotection. the major results are as following : two cdna sequences encoding violaxanthin de - epoxidase were cloned from japonica rice ( jrvde ) and indica rice ( irvde ) with the full - length of 1887bp and 1647bp, respectively. the homology of the open reading frame is 98 % identity between two rvde genes, and more than 60 % identities with those of other species

    本論文從水稻和菠菜中克隆了編碼vde酶的基因，並通過轉基因植物進一步研究了葉黃素循環在熱耗散方面的作用，主要獲得了以下結果：首次從兩個水稻亞種（秈稻和粳稻）中克隆了rvde基因（分別命名為irvde和jrvde ）的全長cdna序列，分別長1647bp和1887bp ，兩者開放閱讀框的同源性為98 ，與其它已知vde基因的同源性在60以上。
  9. The results of sequencing showed that jl94 isolate complete gene 6 was 1356bp and had a complete open reading frame which encoded 397 amino acides

    對克隆的vp6基因進行序列測定，測序結果顯示jl94vp6基因全長1356bp ，含有完整的開放閱讀框架，編碼397個氨基酸。
  10. This sequence emergences fourteen times from 1000 ests library indicts that it is a middle affluently gene in cdna library. the cdna of 634 basepairs contains an open reading frame of 339 nucleotides encoding a novel nonspecific lipid transfer protein. the first 23 amino acids constitute the putative signal peptide, characteristic for targeting to the secretory pathway

    測得th - nsltp序列全長為634bp ，含有一個非特異性脂轉移蛋白與植物耐逆性的相關性研究編碼112個氨基酸的閱讀框架， n端的23個氨基酸組成一段信號肽序列，表明它可能和分泌有關。
  11. It suggested that 1118 contigs were homologous to the genes identified previously by blast from the internet while other 533 contigs matched unknown protein coding regions ( in which 101 had been found before in other organisms and 432 were completely novel )

    網上blast比對顯示其中1118個序列與已知基因相似，其餘533個為未知功能的開放閱讀框（其中101個已在其它生物中發現， 423個為新發現的序列） 。
  12. Badh cdna ( 1901bp ) included a 66 bp 5 " utr, a 329 bp 3 " utr and a 1506 bp orf encoding a 501 - ammo - acid polypeptide which showed 88 % sequence identity to badh from spinach, sugar beet and atriplex hortensis respectively. the deduced amino acid sequence included a decapeptide sequence " vtlelggksp ", which is highly conserved among general aldehyde dehydrogenases ( aldh ), and a cysteine residue

    Badhcdna全長1901bp ， 5端非編碼區66bp ， 3端非編碼區329bp ，含有2個可能的加polya信號： aataa ，開放閱讀框架1506bp ，編碼一個由501個氨基酸構成的多肽，與菠菜、甜菜、山菠菜badh的氨基酸序列同源性均為88 ，其中有醛脫氫酶的保守序列vtlelggksp和半胱氨酸殘基。
  13. The result of the agarose gel electrophoresis showed that the length of the full - length cdnas in the library was pooled mainly between 500 and 2 000 base pairs

    結果表明獲得的ejoi基因的cdna長度為876hp ，開放閱讀框長度為759hp ，編碼252個氨基酸。
  14. The full - length cdna sequence was finally generated by 5 ' race and 3 ' race respectively. at the same time, structure and function of ejol gene were primarily analyzed by bioinformatics method

    結果表明獲得的ejo3基因的cdna長度為1514hp ，開放閱讀框長度為1368hp ，編碼456個氨基酸。
  15. Human gnt - v contains 741 amino acids with six potential sites for n - glycosylation and bears high homology to gnt - v of rat. its gene is located on chromosome 2q21 containing 17 exons. gnt - v protein is encoded by exons 2 - 17 as open reading frame

    人類gnt - v由741個氨基酸組成，有6個潛在的n -糖基化位點，基因定位於染色體2q21 ，含有17個外顯子，其開放閱讀框架由外顯子2 - 17進行編碼。
  16. In order to investigate the genomic organization of the single - nucleocapid nucleopolyhedrovirus of helicoverpa armigera, the ecori - n fragment located at 54. 8 - 59. 3 kbp of the viral genome was sequenced. the fragment contained 3762 bp helicase gene potentially encoding a protein with a molecular mass of 146 kda

    對棉鈴蟲單核衣殼核多角體病毒（ helicoverpaarmigerdsingle - nucleocapsidnucleopolyhedrovirus ， hasnpv ）基因組中ecori ? n片段進行序列分析，獲得了完整的解螺旋酶基因（ hel ） ，其開放閱讀框大小為3762bp ，編碼一個分子量為146kda的蛋白質。
  17. The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size. computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70. 3 % that would encode a protein of 552 amino acids ( aa ). the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp

    對phz1754進行外切核酸酶（ exonuclease ， exo ）順序缺失，獲得單向長度漸減重疊的系列突變體，核苷酸序列測定顯示出該ecor - sal片段的精確大小為2299bps ， frameplot程序分析揭示出該區域一個完整的開放閱讀框（ orf ）的存在，其大小為1656bps ， g + c含量為70 . 3 ，編碼552個氨基酸，利用blastsearch程序將orf的核苷酸序列及推導的氨基酸序列與因特網上基因及蛋白質數據庫進行綜合比較，發現無論在核苷酸水平還是在蛋白水平上，該orf均與膽固醇氧化酶表現出同源性，而且與鏈黴菌膽固醇氧化酶同源性最高，說明該orf編碼膽固醇氧化酶基因。
  18. Pcr product was cloned to the downstream of gst gene according to the right open reading frame ( orf ) in pgex - 6p - l vector, and e. coli bl21 was transformed by the recombinant plasmid for expression

    Pcr產物經純化、酶切后，按正確的閱讀框架定向克隆到表達性載體pgex - 6p - 1中谷胱甘肽轉移酶（ gst ）基因的下游。
  19. Nucleic acid sequences of azoreduclase were searched and blasted in genbank. a pair of primers based on the conserved regions were designed. a specific fragment was amplified by pcr from the plasmid of rhodopsedomonas palustris and sequenced. the sequence contained a complete 471bp orf ( open reading frame )

    脫色實驗證明沼澤紅假單胞菌（ rhodopsedomonaspalustris ）對偶氮染料有較強的降解能力，我們通過genbank搜索，對所獲得的所有偶氮還原酶基因在ncbi進行比對並設計引物，從沼澤紅假單胞菌質粒中擴增獲得了一條含471bp完整開放閱讀框架的序列。
  20. Three kinds of integron were detected from 7 s. typhimurium with a size of 638bp, 1913bp and 2013bp respectively. the products were cloned and sequenced. the results showed that they contained two aada2 gene, one dhfrxii gene associated with the resistance

    序列分析表明，這7株鼠傷寒沙門氏菌的型整合子可分為三種類型，一種大小為638bp ，存在1個閱讀框；另一種大小為1913bp ，含1個aada2 、 1個dhfr基因盒和1個閱讀框；第三種大小為2013bp ，有1個aada2基因盒和1個閱讀框。
分享友人
分享到 Line

分享到臉書