限制酶定位 的英文怎麼說

中文拼音 [xiànzhìdìngwèi]
限制酶定位 英文
restriction enzyme mapping
  • 限 : Ⅰ名詞（指定的范圍; 限度） limit; bounds Ⅱ動詞（指定范圍, 不許超過） set a limit; limit; restrict
  • 制 : Ⅰ動詞1 （製造） make; manufacture 2 （擬訂; 規定） draw up; establish 3 （用強力約束; 限定; 管束...
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 定 : Ⅰ形容詞1 （平靜; 穩定） calm; stable 2 （已經確定的; 不改變的） fixed; settled; established Ⅱ動詞...
  • 位 : Ⅰ名詞1 （所在或所佔的地方） place; location 2 （職位; 地位） position; post; status 3 （特指皇帝...
  • 限制 : place [impose] restrictions on [to]; astrict; restrict; limit; confine; shut down on [upon]: 限制...
  1. Restriction enzymes and dna probes are used to determine the exact sequence, but it is possible to get a rough estimate by analyzing the frequency of recombination between the alleles of linked genes

    雖然可以利用限制性內切酶和dna探針精確的分析出特定序列，但是在分析連鎖基因等位基因間的重組序列時，會出現不準確的估計。
  2. It enables a specific gene to be located on a particular restriction enzyme fragment.

    它就能使專一的基因被定位於特定的限制性內切酶切成的片段上。
  3. After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence

    F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。
  4. Sinensis and e. j. hepuensis has been found in the sequences of the portions of 16s rdna and pcr / rflp studies of 110 samples, from six river valleys in eastern mainland of china. these subspecies - specific restriction sites allow rapid discrimination with the endonuclease dra i, and therefore can be used as a diagnostic genetic marker for identification of the two subspecies

    通過對中國大陸東部6個水系110個絨螯蟹個體16srdna部分序列的測定和pcr rflp分析，發現在合浦絨螯蟹與中華絨螯蟹之間存在3 4個固定的堿基替代，這種亞摘要種特異性的限制性位點可以通過限制性內切酶dra進行快速檢測，成為2個亞種的分子鑒定標記。
  5. 374 - nt sequence analysis between nt 47 - 420 and restriction enzyme ( re ) clevage site mapping of f gene between nt 34 - 1682 were used to compare the 18 isolates for genetic analysis. a phylogenic tree was constructed based on the 374 - nt - sequence data of eighteen isolates in the study and 37 ndv reference strains from genbank and published resources

    通過dnastar軟體對f基因47 470nt間片段進行同源性分析比較並繪制了遺傳進化樹枝狀結構發生圖，結合334 1672nt間三種限制性內切酶（ re ： hinf ， bsto及rsa ）位點的分佈情況，確定了這些分離株的基因分類地位。
  6. At the same time, vp4 gene was mutated in a certain point by pcr. the plant expression vector : pbi121 was constructed and then transformed to agrobacteriutn tumefaciens eha105 directly. a. tumefaciens eha105 harboring pbi121 / vp4 was used to transform the boechmeria nivea l. guad according to the leaf disc procedure

    為便於基因操作，對外殼抗原蛋白vp4基因進行適當的修飾和改造：通過引物設計，利用pcr反應，在基因的起始編碼前引入有助於真核生物表達的kozak序列和限制性內切酶位點；使用套疊pcr對vp4基因進行定點突變，以便於將改造后的基因插入pbi121構建植物表達載體，通過直接轉化法，把pbi121 vp4轉入農桿菌eha105 ，構建了農桿菌工程菌。
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