陽性基 的英文怎麼說
中文拼音 [yángxìngjī]
陽性基
英文
positive group-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。( 2 ) gene flow frequency was reduced as distance from pollen donor increased and a dramatic reduction occurred at about 2 meters. the maximum distance where gene flow was not detected was 50 m for hybrid rice while it was 70 m for ms lines, with an exception that in one of the four ms lines it was detected a frequency of gene flow 2. 8 + 10 - 6 at 150 m for zhong 9a
在開花期主流風向ne的風速為0 . 2 ? 2 . 2m / sec的條件下， 2個雜交稻品種的最大漂流距離為40m ； 4個不育系的基因漂流基本上到60m為止， 70m處基因漂流頻率均降為0 ，僅中9a在150m處發現了1株basta抗性苗，經pcr檢測驗證為陽性。Transformed shoots were selected on solidified medium containing kanamycin. ten kanamycin resistant transformants were obtained by direct or induction calla. these transformants were checked by pcr, pcr - southern blot and southern blot, confirmed that two positive transformants were integrated into the genome of boechmeria nivea l guad
對所得到的抗性轉化株進行了pcr 、 pcr - southernblot 、 southernblot檢測，其中2株呈陽性，證明vp4基因已整合到苎麻基因組dna中。Explants were then transferred onto the selection medium containing 500mg / l carbenicillin and loomg / l kanamycin and incubated at 25, 16 / 8h light / dark cycle. small leaves of adventitious shoots differentiated from explants were cut and dipped into gus staining solution. positive shoots, gus tinted, were induced to root
分化出的不定芽切下半張葉片進行gus染色，呈陽性的植株從外植體上切下，在生根培養基（ ms 0刀sing lnaa 50mg幾kan ）中誘導根的形成。Finally 4 right clones was obtained from 864 transformants by pcr detection. sequencing analysis showed that the hsa gene have been introduced into the right position of the human a - lactalbumin yac. furthermore, works to optimize the conditions of introducing the recombinant yac into goat f ibroblast via cell fusion was explorded
經pcr檢測，從864個轉化子中獲得了4個陽性克隆，測序表明，人血清白蛋白基因已正確的導入到人-乳白蛋白基因yac的特定位點上，並獲得了可進行細胞融合的重組yac載體。In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli
根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。Milk and milk - based products - detection of thermonuclease produced by coagulase - positive staphylococci
乳和以乳為基料的製品.凝固酶-陽性葡萄球菌的耐熱核酸酶檢測Results the hyperplastic basal cells were characteristically larger than normal, with oval or spindle nuclei, and formation of double layer - glandlike, solid or cribriform patterns, stained positive for 34 e12
結果顯示增生的基底細胞比正常增大，核呈卵圓形或梭形，常呈雙層腺樣、實體和篩狀結構和34 e12陽性反應特徵。Examination of defecate occult blood is method of very convenient a kind of assay, the laboratory of unit of basic level medical treatment all can undertake, to doubtful large intestine carninomatosis person, should relapse in time for many times occult blood of ground assay defecate, old people is annual when medical, also should consider to have this laboratory test, experiment of patient defecate occult blood often is large intestine electropositive
大便潛血檢查是很方便的一種化驗方法，基層醫療單位的化驗室均可以進行，對可疑大腸癌病人，應該及時反復多次地化驗大便潛血，老年人每年體格檢查時，也應該考慮進行這項化驗，大腸癌的病人大便潛血試驗經常為陽性。Distribution and source of calcitonin gene - related peptide immunoreactive nerve terminal in prepuce of penis and frenulum of prepuce of rats
大鼠陰莖包皮及包皮系帶內降鈣素基因相關肽免疫陽性神經末梢的分佈與起源In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。In normal greenhouse condition, coda - transgenic wheat lines ( to ) had the same plant morphorlogy and pollen i2 - ki staining rate as untransformed control plant. after treating with 300 mm of 5 - fc, however, changes in configurations of spikelet, floret and anther have been observed in the transgenic lines but not in the control, and 50 % gus - positive lines displayed outside - opened glume, abnormal stamen, smaller and thinner anther, shorter filament, and failure of selfing. in parts of 5 - fc - treated transgenic lines, the pollen staining rate by i2 - ki was much lower than that of untransformed control
溫室栽培的轉基因小麥苗（ t _ 0 ）未噴5 - fc處理時植株外部形態和花粉碘-碘化鉀染色的著色率與未轉基因的對照沒有差異；用300mm的5 - fc處理后，發現有50 gus陽性株系與對照有明顯的區別，表現為小穗穎殼外張，花絲短縮，花藥發育不良，較小、黃白色且花粉粒少，自花不授粉，無外來花粉授粉則不結實。Based on the residue monitoring results in the previous year, especially on the feedback information of the positive ( non - compliance ) results, and taking account of world - wide alert notifications, samples of some items to be taken will be duly increased year by year ; based on the reasonable suggestions in fvo inspection report and the problems of chloramphenicol and nitrofuran which had been notified by eu in recent years, the monitoring items are being added in order to meet the residue requirement of importing countries and regions such as the eu, japan, korea, switzerland and hk
國家質檢總局根據上一年度殘留監控結果反饋情況，特別是陽性結果的檢出情況，結合各國預警通報的情況，每年適當增加對應監控的檢測項目的取樣數量；尤其是針對歐盟每次考察報告中對我殘留監控計劃提出的合理化建議和近年來歐盟通報的氯黴素和硝基呋喃問題，增加了滿足歐盟、日本、韓國、瑞士、香港等國家和地區殘留監控要求的項目。Standard test method for measurement of thickness of anodic coatings on aluminum and of other nonconductive coatings on nonmagnetic basis metals with eddy - current instruments
用渦流儀器測量鋁陽極鍍層及其它非磁性基底金屬絕緣鍍層的厚度標準試驗方法Clinical symptoms associate cerebellous ataxia, pyramidal syndrome with babinski sign, abolition of reflexes, disorder of profound sensitivity, dysarthria, oculomotor disorders and cardiomyopathy
臨床癥狀伴有：小腦共濟失調，椎體綜合征，如巴賓斯基陽性，反射消失，深度反應失調，構音障礙，眼球運動障礙，以及原發性心肌疾病。The result showed that the homology rate of pila gene among the 5 avian pathogenic e. coli strains tested and one human e. coli were from 89. 8 % to 91. 1 %, and the homology rate of amino acid were from 88. 5 % to 91. 8 %. the homology rate of pila gene sequence among 5 avian pathogenic e. coli strains tested and avian pathogenic e. coli reported ( serotype o1, o2, o78 ) were from 87. 8 % to 90. 2 %, and the homology rate of amino acid were from 84. 6 % to 91. 2 %. there had homology in avian pathogenic e. coli. there had some common antigen side in type 1 pili of avian pathogenic e. coli
結果表明：運用msha法檢測1型菌毛的檢出率為80 （ 36 45 ） ， pcr法的檢出率為95 . 5 （ 43 45 ） ， pcr方法用於1型菌毛的檢測比msha更加敏感、快速、特異性強；選擇5株優勢血清型雞源致病性大腸桿菌代表株（ o _ （ 89 ） ， o _ （ 119 ） ， o _ （ 141 ） ， o _ （ 127 ） ）的1型菌毛pila基因的pcr擴增片段經純化后，分別定向克隆到puc18質粒的多克隆位點，構建了含有目的基因片段的克隆質粒，並轉化到dh5株大腸桿菌載體菌中，篩選獲得陽性克隆菌株。The individuals of rhd - positive phenotype with intact exons carried generally insert fragments and boxl box2 and box3 and this proved that inserts or rh box could n ' t affect the express of rh d gene. in 2 of the 5 wei nationality pedigrees whose proband were rh d - negative, rhc / e phenotype of all the rh negative individuals was ccee. rhd exon 4nsert and rh box did not be found in all individuals
在7個先證者為rhd陰性的漢族家系中，大部分成員均出現插入片段和rhbox ，且在遺傳上符合孟德爾遺傳定律， d外顯子完整且表型為rhd陽性的家系個體成員廣泛帶有插入片段和box1 、 box2或box3 ，插入片段或rhbox並未影響d基因的表達。Using the hprt gene as a positive control, our result suggested that both the testis tissue and the male embryos from which sry transcription can be detected failed to yield any positive results of xist. female embryos at the pronucleus stage and 2 - cell failed to produce any positive result of sry and xist too. while since the 4 - cells period, xist is constantly transcribed until blastocyst stages
然後利用實驗一確定的pcr條件，以hprt為陽性對照，用巢式rt - pcr對小鼠早期胚胎進行xist基因的轉錄分析，結果發現，轉錄sry基因的睪丸組織以及雄性胚胎，從受精卵發育到囊胚的過程中，基本上不轉錄xist基因；不轉錄sry基因的雌性卵母細胞和雌性胚胎，從出現原核開始，到發育至2 -細胞期的過程中， xist基因一直不轉錄，但是，從4 -細胞期開始，一直到孵化前囊胚階段，雌性胚胎都轉錄xist基因。In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported
在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。Results 1. the expression of nf b p65 and i e in skin contusion repair in the control specimens, the positive staining of nf b p65 and i ba were in the basal cell layer, spinous layer, granular layer and sweat epithelium, sebaceous gland epithelium
實驗結果1 . nfkbp65與ikba在挫傷修復過程中的表達變化正常對照組皮膚中， nfkbp65與ikba在表皮基底細胞層、棘細胞層、顆粒細胞層和真皮層皮脂腺、汗腺上皮細胞呈陽性表達。分享友人