陽明病 的英文怎麼說
中文拼音 [yángmíngbìng]
陽明病
英文
yangming disease-
According to coming from spanish report, the person that medicaments of the abuse inside hemophiliac, vein becomes addiction and blood are dialytic patient, rate of positive of antibody of third hepatitis virus is respectively 64 %, 70 % reach 20 %, more average crowd is apparently tall
據來自西班牙的報告，血友病患者、靜脈內濫用藥物成癮者及血液透析患者，丙型肝炎病毒抗體陽性率分別為64 % 、 70 %及20 % ，較一般人群明顯為高。While disease had thus become an inhabitant of lowood, and death its frequent visitor ; while there was gloom and fear within its walls ; while its rooms and passages steamed with hospital smells, the drug and the pastille striving vainly to overcome the effluvia of mortality, that bright may shone unclouded over the bold hills and beautiful woodland out of doors
就這樣，疾病在羅沃德安了家，死亡成了這里的常客圍墻之內籠罩著陰郁和恐怖房間里和過道上散發著醫院的氣味，香錠徒勞地掙扎著要鎮住死亡的惡臭。與此同時，五月的明媚陽光從萬里無雲的天空，灑向陡峭的小山和美麗的林地。You if tuberculin is electropositive, can explain you had affected n / med tuberculosis bacterium only, whether to get tuberculosis, still need by the doctor integrated your clinical expression, phlegmy fluid is bacteriological examine and x line examination undertake an analysis, make final judgement
您假如結核菌素陽性，只能說明您已經感染了結核菌，是否得了結核病，還需由醫生綜合您的臨床表現、痰液細菌學檢驗和x線檢查等進行分析，作出最後判定。The high specificity of the dot - ppa - elisa was confirmed by specific blocking test and also by cross - reaction test. the diaphragm did not react with the antibodies against salmonellosis, streptococcosis, colibacillosis, chlamydiosis, hcv, ppv, prv, brucellosis. erysipelas, suis and chlamydiosis in cross - reaction test. the diaphragm has good sensitivity and could detect some pasteurella - positive test serum which has been diluted to2 - "
試驗證明所建立的dot - ppa - elisa具有較好的特異性，與豬瘟、仔豬副傷寒、豬丹毒、豬細小病毒病、豬偽狂犬、豬布氏桿菌病、豬衣原體病陽性血清無交叉反應。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。If you decide exterior antibody is masculine gender, that is too good, explain you do not have sicken at all, and you still have certain immune power to second liver
假如你確定表面抗體是陽性的話，那就太好了，說明你根本沒有患病，而且你對乙肝還有一定的免疫力。The result showed that the homology rate of pila gene among the 5 avian pathogenic e. coli strains tested and one human e. coli were from 89. 8 % to 91. 1 %, and the homology rate of amino acid were from 88. 5 % to 91. 8 %. the homology rate of pila gene sequence among 5 avian pathogenic e. coli strains tested and avian pathogenic e. coli reported ( serotype o1, o2, o78 ) were from 87. 8 % to 90. 2 %, and the homology rate of amino acid were from 84. 6 % to 91. 2 %. there had homology in avian pathogenic e. coli. there had some common antigen side in type 1 pili of avian pathogenic e. coli
結果表明：運用msha法檢測1型菌毛的檢出率為80 （ 36 45 ） ， pcr法的檢出率為95 . 5 （ 43 45 ） ， pcr方法用於1型菌毛的檢測比msha更加敏感、快速、特異性強；選擇5株優勢血清型雞源致病性大腸桿菌代表株（ o _ （ 89 ） ， o _ （ 119 ） ， o _ （ 141 ） ， o _ （ 127 ） ）的1型菌毛pila基因的pcr擴增片段經純化后，分別定向克隆到puc18質粒的多克隆位點，構建了含有目的基因片段的克隆質粒，並轉化到dh5株大腸桿菌載體菌中，篩選獲得陽性克隆菌株。The epidemiological investigation of deer brucellosis in part of province and area demonstrated that brucellosis was universal in raising sika deer and wapiti in china, the positive ratio in female deer was higher than male deer ; the positive ratio respectively was 10. 06 % 、 4. 77 % in sika deer and 11. 15 % 、 4. 99 % in wapiti
摘要對我國部分省區鹿布氏桿菌病進行流行病學調查表明，我國鹿場飼養的梅花鹿、馬鹿群中普遍存在鹿布氏桿菌病，母鹿陽性率明顯高於公鹿，梅花鹿陽性率分別為10 . 06 % 、 4 . 77 % ；馬鹿陽性率分別為4 . 90 % 、 11 . 15 % 。Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays
為了便於轉基因棉花後代的篩選，在pap基因的3 』端融入了綠色熒光蛋白gfp ）基因，然後將融合基因克隆在植物表達載體pbi121上，再進行遺傳轉化，得轉基因棉花種子5000餘粒，將種子播種長到于葉展開時，先在黑暗中用紫外燈照射，查找表現綠色熒光的幼苗，然後再用地高辛（ dig ）標記的pap基因特異性探針對這些棉花進行點雜交，最後發現有8株棉花表現陽性反應，說明pap基因的確己經轉到了棉花的基因組中，其棉花黃萎病的抗性鑒定正在進行之中。The env protein deduced from env gene encodes the hydrophilic surface protein ( su ) and the hydrophobic transmembrane domain ( tm ) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry. the su of retroviruses is a highly variable genetic element, containing receptor binding sites and major antigenic determinants. exjsrv - specific dna probes were derived. by using these dna probes in tissue hybridization. we successfully identified jsrv mrna expression and proviruses dna in sheep lung tissues infected with jsrv and control group has no postive signals, validating the use of exogenous virus - specific dna probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences
用地高辛隨機引物法標記exjsrv特異的env片段，制備探針，原位雜交檢測spa肺組織中的rna及前病毒dna ，結果表明spa患羊肺組織內有jsrvenv基因mrna的表達，同時也檢測到了前病毒dna ，而相應的陰性對照卻無陽性信號，證實外源性病毒特異的dna探針在致瘤性前病毒的整合位點和整合的外源性前病毒的檢測中具有可信度。At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged
在本實驗中，利用隨機12肽庫對抗豬瘟病毒（ classicalswinefeverviruscsfv ）糖蛋白me2的單抗a11進行表位篩選，經過四輪篩選以後，隨機挑取11個克隆作競爭- elisa檢測，結果表明，所挑11個克隆中，有9個克隆能對me2蛋白和a11反應產生抑制作用，抑制率最高可達64 ； dna測序以後經過dnastar軟體分析，發現它們的核心序列為anwralsl ，該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別；人工合成含核心序列的多肽經間接elisa試驗證實，也能被a11識別。Results showed that the temperature distribution of the traditional embankment was asymmetrical ; the asymmetrical status was changed after ripped - rock revetment was installed and the soil under the ripped - rock was cooled obviously ; the ripped - rock revetment could cool embankment and adjust the temperature difference between the north - facing slope and the south - facing slope ; the ripped - rock revetment was an available technique to protect embankment from thawing settlement and longitudinal cracks in permafrost regions
結果表明：普通路基的陰陽坡兩側溫度分佈極不對稱；鋪設拋石護坡后，這種狀況得到了很大改善，並且拋石護坡下土體溫度明顯降低；拋石護坡能夠降低路基溫度和調節路基陰陽坡的溫度差異；拋石護坡是多年凍土區防治路基融沉和縱向裂縫病害的一種可行措施。Two strains of prrsv were isolated from the swine infected with prrsv in shangdong province and daqing area, in order to clarify the source and genetic background of porcine reproductive and respiratory syndrome virus ( prrsv ) from different parts of china, thus providing theoretic basis for the study of vaccine against it. the prrsv was cultured on mark - 145 cells for 5 ~ ~ 6 passages. when the cpe was obvious, the virus was harvested and purified
為了弄清我國不同地區prrsv的來源以及其遺傳學背景，為疫苗學研究提供理論根據，本研究在ch - 1a株完整的基因組獲得以後，從流行於我國山東（ sd ）和黑龍江大慶（ dq ）地區疑似prrs的豬體內分離到prrsv ，在mark - 145細胞上盲傳5 6代，細胞出現明顯病變以後，收獲病毒液，然後提純，提取全病毒rna ，經過反轉錄、 pcr擴增獲得結構基因orf2 7的目的基因片斷，然後與pmd - t載體連接，轉化，得到陽性質粒后進行測序，並將其與ch - 1a株進行了比較分析，同時對這兩個毒株的結構基因組的理化性質進行分析。The centre for health protection of the department of health today november 18 received a report from the university of hong kong that laboratory tests using reverse transcription polymerase chain reaction rt - pcr method on three out of the seven specimens taken from patients of the caritas medical centre outbreak yielded positive results to parainfluenza virus, but all the seven specimens were negative for other common respiratory pathogens
生署生防護中心今日十一月十八日收到香港大學的報告，明愛醫院病童的七個樣本經大學完成反轉錄-聚合鏈反應rt - pcr測試后，其中三個樣本證實對副流感病毒呈陽性反應，但是所有七個樣本均對其他常見的呼吸道感染病原體呈陰性反應。The interesting gene fragment with ecori and noti were amplified by overlapping pcr, which inserted into vector plasmid ppic9k after degisted by ecori and noti, and the recombinant plasmid was transformed into competent dh5cc. positive clones were screened by pcr from the lb plate with amp. digesting analysis resulte shows that the interesting gene were inserted into the vector ppic9k with correct direction
目的基因經雙酶切后連接載體ppic9k ，然後導入大腸桿菌dh5中，在含氨卞青霉素（ amp ）的lb板上用pcr反應篩選出陽性菌落，雙酶切結果表明目的基因已插入載體中，且方向正確，測序結果進一步證明人巨細胞病毒重組基因表達質粒成功地克隆了目的基因片段。Re - thinking of yangming diseases
對陽明病的再認識Relationship between yangming disease and epidemic febrile disease in practical treatise on febrile diseases
陽明病與溫病關系的探討The expression conditions of e2 gene in p. pastoris were optimized, the results indicated that the peak obtained after 72 hours ; pattern of inhibition / induction could improve expression level ; the best ph value were between 7. 5 and 8. 0 and the optimized methanol - induced concentration was 2 % - 3 % the e2 genes of the prevalent strain ( guangxi yulin strain ) and c strain derived from rabbit spleen tissue were amplified and cloned into e. coli the expression vector pproex - htb respectively, the recombinant plasmids pproex - gxyl and pproex - c were obtained and then were transformed into the dh5a e. coli competent bacteria respectively, the recombinant bacteria could express the major antigen region of e2 gene, the expression yields amount to 35 % and 38 % repectively
豬瘟病毒ez基因的原核表達： pcr擴增出當前豬瘟流行野毒株，中國豬瘟兔化弱毒（ c株）兔脾組織毒ez基因的主要抗原區，將其克隆到原核大腸桿菌表達載體pproex htb中誘導表達，經sds page檢測表明，重組質粒能表達ez基因主要區蛋白， westernblot檢測表明，誘導表達蛋白與豬瘟陽性血清發生特異性反應，表達量為35和38 ，可用於基因工程診斷抗原。With simple materials like leaves, cottons and swab, is it possible to create amusing photo work ? local well - known photographer tseming - chong has shared such experience to the patients in the alice ho miu - ling nethersole hospital and the queen elizabeth hospital during a series of sun print workshops. the sun prints created by these patients are displaying their imagination as well as how the process of sun prints is pleasantly surprised
藝術在醫院邀請資深攝影工作者謝明莊先生，于伊利沙伯醫院及雅麗氏何妙那打素醫院擔任攝影工作坊導師，教導病人如何利用各類隨手可得的材料，如樹葉、紗布、綿花等及利用太陽光創作出別出心裁的sun print攝影作品。The specificity of the nucleic acid probe was very strict. lt reacted positively with iltv dna only and it react negatively with the nuleic acid of ndv, bv and ibdv. the sensitivity of this kind of probe is very high. ilt could even detect 20pg ' s iltv dna
結果表明：該種核酸探針具有高度的特異性，它僅與iltv的dna呈現陽性反應，而與新城疫病毒、傳染性法氏囊病病毒和傳染性支氣管炎病毒的核酸等均呈陰性反應。該種探針具有高度的敏感性，能夠檢測到20pg的iltv的dna 。分享友人