隆中對 的英文怎麼說
中文拼音 [lōngzhōngduì]
隆中對
英文
longzhong plan-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。These findings are “ a very important result in resolving one of the most difficult problems related to carbon nanotube manufacture for integrated circuits, ” says kang wang, director of the center on functional engineered nano architectonics at the university of california, los angeles
美國加州大學洛杉磯分校功能性改造奈米結構體系專研中心主任王康隆表示，這些發現對解決在積體電路中製造奈米碳管的難題來說，是非常重要的結果。And she was wondrous stricken of heart for that evil hap and for his burial did him on a fair corselet of lamb s wool, the flower of the flock, lest he might perish utterly and lie akeled for it was then about the midst of the winter and now sir leopold that had of his body no manchild for an heir looked upon him his friend s son and was shut up in sorrow for his forepassed happiness and as sad as he was that him failed a son of such gentle courage for all accounted him of real parts so grieved he also in no less measure for young stephen for that he lived riotously with those wastrels and murdered his goods with whores
時值隆冬，伊惟恐亡兒凍僵，屍骨無存，遂以通稱為羊群之花的小羊羔毛制一精緻胸衣，裹于兒身。利奧波德爵士失卻嗣子后，每當目睹友人之子，即懷念往日之幸福，遂沉浸於凄楚之中。悲的固然是與心地如此善良之子嗣永別眾人皆對彼之前途寄予厚望焉，亦同樣為青年斯蒂芬哀傷，蓋彼與諸蕩兒為伍，飲酒狂鬧，將財產糟踏在娼妓身上。Many organisms produce enzymes, termed photolyases, that specifically bind to these damage products and split the via a uv - a / blue light - dependent mechanism ( photoreactivation ), thereby reversing the damage. these two photolyase are specific for either cpds ( cpd photolyase ) or 6 - 4 products ( 6 - 4 photolyase ). a gene that expresses a protein with 6 - 4 photolyase activity in vitro, was recently cloned from high organisms ( arabidopsis thaliam, drosophila melanogaster, danio rerio, xenopus laevis and homo sapiens )
目前已從高等生物擬南芥、鮐類、果蠅、人類和非洲爪蟾蜍屬中克隆到有（ 6 - 4 ）光裂合酶活性的基因，本研究從鹽生杜氏藻dunaliellasalina中克隆到（ 6 - 4 ）光裂合酶的基因，並將該基因在大腸桿菌中得以表達，這是首次在藻類中克隆到（ 6 - 4 ）光裂合酶基因，對光裂合酶的研究具有重要意義。The study results of this paper can serve the two scientific subjects of our teaching and research group as basic data calculation and elementary exploration. the two subjects are : constructing high activity - amylase genes by dna shuffling technology and studying on the evolution in vitro by mutational pcr ( with 5 - br - dutp as substitute partly ) and dna shuffling technology. - amylase ( ec 3. 2. 1. 1 ; 1, 4 - a - d - glucanohydrolase ) can catalyzes the hydrolysis of - 1, 4 - glycosidic bonds of starch from middle and liberates - maltose, - glucose and - limit dextrin stepwise
本試驗根據genbank已公布的黃單胞菌-澱粉酶基因的核苷酸序列由引物設計軟體premierprimer5 . 0輔助設計了一對引物（ primer & primer ） ，以pbluescript ks +和puc18 / puc19質粒為載體，用常規的pcr方法從xanthomonascampestrispv . malvacearum （ smith ） dye等七株黃單胞菌（ xanthomomasspp . ）的基因組dna中克隆得到8個基因片段，分別命名為zhyf001 zhyf008 。Highlights of harbin include colorful and mysterious ice lanterns, crystal - clear ice carvings and pure - white snow sculptures of exquisite craftsmanship. sport - lovers may choose to ride a wind - driven snow sledge with sail to tear along the snow - covered fields or make a jump into the icy water, or go to the yabuli ski resort by a new expressway. for newly - weds, they may attend a grand group wedding ceremony held during the joyful ice and snow festival
這里：冰燈，五光十色，神奇詭秘冰雕，玲瓏透剔，光彩照人雪塑，潔白無瑕，晶瑩如玉體育健兒駕著冰帆在雪原飛馳冬泳健兒在冰水中揮臂前進一對對新娘新郎在歡樂的冰雪節游園會上舉行隆重的集體婚禮喜愛滑雪運動的遊人正乘車沿著高速公路直奔亞布力滑雪場處處豪情壯舉，處處歡聲笑語，譜寫著春天的序曲。Furthermore, the wing imaginal disc clonal analysis showed that in the homozygous sll mutant clones, the extracellular wg was dramatically reduced. the reduction of extracellular wg indicated that the wg signaling in the wing imaginal disc was disrupted. the reason leaded to this phenomenon is that sll encodes a paps transporter, so the disruption of the sll gene would generate the unsulfated heparan sulfate proteoglycan ( hspg ), which most likely would lose normal function
進一步結合運用flp - frt系統和minute ~ - ，用缺損myc蛋白的表達來標記純合的sll突變基因克隆，用minute ~ -來相對的擴大含sll突變基因的克隆，對翅成蟲盤所作的克隆分析表明，在含sll突變基因的克隆中，細胞外的wg蛋白分佈明顯減少，這種細胞外的wg蛋白減少表明在翅成蟲盤中wg信號傳導受到了明顯抑制。At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged
在本實驗中，利用隨機12肽庫對抗豬瘟病毒（ classicalswinefeverviruscsfv ）糖蛋白me2的單抗a11進行表位篩選，經過四輪篩選以後，隨機挑取11個克隆作競爭- elisa檢測，結果表明，所挑11個克隆中，有9個克隆能對me2蛋白和a11反應產生抑制作用，抑制率最高可達64 ； dna測序以後經過dnastar軟體分析，發現它們的核心序列為anwralsl ，該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別；人工合成含核心序列的多肽經間接elisa試驗證實，也能被a11識別。On the hh signal receiving cells, the function of hspg in hhn movement might be that it competes with ptc for binding of hhn, following the releasing from the disp, hhn binds to the hspg to prevent the hhn from being captured by the ptc, hence facilitate it ' s transfer to the more distantly located cells
進一步結合運用flp frt系統，用缺損gfp蛋白的表達來標記純合的oxt突變基因克隆，針對眼睛成蟲盤的克隆分析結果表明，在oxt突變克隆中， hh下游的以蛋白的表達區域完全消失。The total rna was purified from the germ in the liquid by the guanidine isothiocyantehod method, then the total rna digested by dnase that had not rnase was used for rt - pcr. i change the magnesium ion dencity in the pcr system in order to optimize the pcr condition. at the end i selected the magnesium ion density as 1. 25 mm. the production of rt - pcr was inserted directionally into pgem ? z ( ampr ). the pgem ? z ( ampr ) was used to transform e coli jm109. i got a positive clone through culling and identificatin. the dna sequence inserted into pgem ? z ( ampr ) was sequenced and blasted with the cdna sequence of the # - mannanase mature peptide that got from genbank
分取誘導培養液中的菌體，用異硫氰酸胍法提取總rna ，總rna再經無rna酶的dna酶處理後用于rt ? pcr 。在pcr擴增目的基因時，通過優選擴增體系，使鎂離子濃度為1 . 25mm時rt ? pcr可順利地獲得目的基因，並能定向克隆到載體pgem ? 3z （ amp ~ r ）中。用克隆載體轉化宿主大腸桿菌jm109 ，通過篩選獲取陽性克隆子，對陽性克隆子進行酶切與pcr鑒定，並對載體中插入的目的基因進行測序。Benjamin t sou, tom lai, samuel chan, and lin hing lung have argued against the traditionally held view that there is no consistency in human textual summarization. in their experiment, they derived a measure and showed there is a fairly reasonable degree of consistency in 150 chinese subjects from both sides of the taiwan straits when summarizing argumentative discourse
鄒嘉彥、黎邦洋、陳偉光和連興隆在文章中對認為人為文本摘要不存在一致性的觀點提出了他們的不同看法。他們以１ ５ ０名海峽兩岸華人作為實驗對象，分組進行社論摘要的抽取實驗，及給出了評價的方法和數學模型。A. d. 207 liu bei repeatedly asies, zhuge liang explains ideas of the three minute worlds to liu bei, namely famous " long zhong dui " 隆 中 對, becomes an official immediately assists liu bei
公元207年劉備三顧茅廬，諸葛亮對劉備陳說三分天下之計，即著名的「隆中對」 ，隨即出山輔助劉備。This includes the boot - floppies installation which has been translated to a number of languages. there is extensive support for french, german, italian, japanese, portuguese, spanish, catalan and danish, and there are more than fifteen active translation teams
系統中對法文德文義大利文日文葡萄牙文西班牙文加泰隆尼亞文以及丹麥文都有廣泛的支援，而現在則有超過十五個團隊正在積極進行翻譯的工作。The routine method for inducing protein function evolution is imitating and speeding protein gene mutation. in this research - amylase gene was mutated in vitro by pcr with dntp in which deoxythymidine triphosphate ( dttp ) was partially replaced by 5 - bromo - 2 ' - deoxyuridine - 5 ' - triphosphate ( 5 - bdu ). the higher the concentration is, the stronger its inducing ability is in a certain range
本研究採用5 -溴脫氧尿苷三磷酸（ 5 - bdu ）部分取代脫氧胸苷三磷酸（ dttp ） ，在pcr的過程中對克隆的野油菜黃單胞菌的-澱粉酶基因進行了體外誘變，誘變結果表明： 5 - bdu濃度越高，誘變越強；濃度越低，誘變越弱。Meanwhile, there was different in the development of embryos reconstructed with various strain oocytes. the activation methods, passages and types of es cells were comparing in mouse cloning
在胚胎幹細胞異種克隆中，比較了激活重構胚的不同方法、不同代數胚胎幹細胞以及不同類型的供核細胞對異種重構胚的影響。Plasmid psyxl, containing a 12kb insert of s. tenebrarius dna, was isolated from one of six colonies. apramycin resistant gene was located in 1. 5 - kb sphl - kpnl fragment of plasmid psyxl though further studies
陽性克隆中重組質粒psyx1含有12kb黑暗鏈黴菌的dna ，對12kb片段進一步分析將安普黴素抗性基因定位在1 . 5kbsphi - kpni片段上。By applying an extended dynamic colonal selection ( dynamics ) approach and the niching technique, this paper presents a maturation detector evolution algorithm to improve the diversity and efficiency of the detectors
摘要基於擴展的動態克隆選擇的入侵檢測模型，在動態克隆中引入小生境技術對成熟檢測器進行進化，以增加檢測器的有效性和多樣性。The 3. 4kb ecori dna fragment of the pgxn201 and the mutant ecori dna fragments of the three mutant plasmids were subcloned into the ecori sites of the clone vectors ml3 and the pgem3z - f ( + ) respectively. through dna sequencing and dna sequence analysis by comparing with the bradyrhizobium japonicum dna sequences reported, we found that there are four intact open reading frames in the 3. 4kb fragment and it was highly homologous to the bradyrhizobium japonicum dna sequence reported, and the tn5gusa5 containing in the pgxn217 was inserted in one of the four open reading frames. this open reading frame located on about 5. 5kb upstream of the nfec gene, which includes 207bp nucleotides, coding 68 putative amino acids and this is completely identical to that of the bradyrhizobium japonicum dna sequence reported
本研究首先利用轉座子tn5gusa5對重組質粒pgxn201進行誘變，獲得3 . 4kbecori片段插入了tn5gusa5的突變質粒pgxn215 、 pgxn216 、 pgxn217 。對這些突變質粒進行亞克隆及測序分析並與基因庫中已報道的慢生型大豆根瘤菌的dna序列作比較，發現突變質粒pgxn217中tn5gusa5恰好插入在一個未知功能的開放閱讀框架內。將pgxn201的3 . 4kbecori片段與m13作連接，獲得亞克隆pgxn201c ，對pgxn201c進行測序分析，發現與基因庫中已報道的慢生型大豆根瘤菌的dna序列有95的同源性。The total dna of exoconjugants acquired dna degradation phenotype during electrophoresis, which suggested that the dnd gene cluster was heterologously expressed. the phz1358 was used sucessfully as a vector for gene replacement to identify the biosynthetic pathway genes for nanchangmycin in s. nanchangensis ns3226. however, the gene replacement in another potential pks cluster has not succeeded till now
將利用phz1358構建的基因置換結構（孫宇暉，未發表）導入南昌鏈黴菌ns3226中進行了基因置換實驗，協助完成了南昌黴素生物合成基因簇的克隆，但對另一潛在pks基因簇的基因置換實驗目前還未能完成。Assaying of the protective effect of mimotopes we divided the mimotopes into three groups based on their amino acid sequences " homology, with phage ml3 as the control group, then immunized 6 - week balb / c mice, 10 mice per group. after amplifing positive phages and quantification, we used them to immunizing mice subcutaneously at 2 - week intervals. the dose given is 100ug phage per mouse at first immunization and 30ug phage per mouse at the latter two immunizations
並且在這12個陽性克隆中，有8個可特異性的與hini多抗結合，可能為二者共有的抗原表位口2 .模擬表位的免疫保護作用分析根據模擬表位的序列相似性分3組免疫6周齡的雌性balb / c小鼠， 10隻/組，以野生噬菌體為陰性對照。分享友人