集成酶 的英文怎麼說
中文拼音 [jíchéng]
集成酶
英文
integrase-
We pay attention to the technical content of the product, carries on the deep processing to the product, introduces the bacteriolysis enzyme extraction technology of american group company, take the duck ' s egg as the raw material, extract the bacteriolysis enzyme that is widely applied to the medicine, the cosmetics, food anticorrosion and the bio - engineering fields of research, and we may extract 2500 kilograms every year ; we study the technology of maintaining egg product freshness with the shenyang agricultural college ; abandons with the national environmental protection agriculture using the project center, the dalian environmental science design research institute, the chwangho city environmental protection bureau jointly carries on the birds and beasts feed increase ecology conditioner expansion tests, makes zhuanghe city heidao agriculture comprehensive exploitation co., ltd the provincial level science and technology innovation enterprise
莊河市黑島農業綜合開發有限公司在產品開發中注重提高產品的科技含量，對產品進行深加工，引進美國艾蒂集團公司溶菌酶提取技術，以鴨蛋為原料，提取廣泛應用於醫學、化妝品、食品防腐及生物工程研究領域的溶菌酶，年可提取2500公斤；與沈陽農業大學聯合研究蛋品保鮮技術；與國家環保農廢利用工程中心、大連市環境科學設計研究院、莊河市環保局聯合進行禽飼料添加生態調節劑擴大實驗，使黑島農業綜合開發有限公司成為省級科技創新型企業。Abstract : numerous mushrooms are toxic to insects. to identify the chemicals involved in insecticidal activity, the toxicity of 14 species has b een studied for water solubility, thermolability, and dialysis. the data strongly s uggest that proteins are responsible for most of the insecticidal avtivity in mu shroom fruitbodies and may be a source of genes available for plant protection a gainst insects. among proteins, lectins and haemolysins are good insecticide candi dates because the toxicities are not affected by protease
文摘:許多蘑菇都對昆蟲表現出毒性.為了證實與殺蟲毒性有關的化合物，對14種蘑菇的毒性在水溶性、熱敏性和可透析性等方面進行了研究.研究數據表明，蛋白質對大多數蘑菇子實體的殺蟲活性起著重要作用，也許是一種可以用於植物抵抗害蟲的基因源.在數種蛋白質中，凝集素和溶血素因不受蛋白酶的影響而成為良好的殺蟲劑候選材料Numerous mushrooms are toxic to insects. to identify the chemicals involved in insecticidal activity, the toxicity of 14 species has b een studied for water solubility, thermolability, and dialysis. the data strongly s uggest that proteins are responsible for most of the insecticidal avtivity in mu shroom fruitbodies and may be a source of genes available for plant protection a gainst insects. among proteins, lectins and haemolysins are good insecticide candi dates because the toxicities are not affected by protease
許多蘑菇都對昆蟲表現出毒性.為了證實與殺蟲毒性有關的化合物，對14種蘑菇的毒性在水溶性、熱敏性和可透析性等方面進行了研究.研究數據表明，蛋白質對大多數蘑菇子實體的殺蟲活性起著重要作用，也許是一種可以用於植物抵抗害蟲的基因源.在數種蛋白質中，凝集素和溶血素因不受蛋白酶的影響而成為良好的殺蟲劑候選材料In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。The results of biological tests have demonstrated that allantoic fluid of the first passage virus did n ' t produce macroscopic pathogenic role to chicken embryos and after passaged for four times, gross lesions were observed in chicken embryo. the virus showed typical coronavirus under electron - microscope and it could n ' t form plaque in cef cells and could hemagglutinates chicken red blood cells after treatment with 1 % trypsin. to surprise, the virus replicated in cef cells also showed hemagglutination activity to chicken red blood cells. in addition, the spf chickens which inoculated with the virus isolated from the chicken damaged tissue showed clinical sign and grow lesion, but it ' s gross lesion did n ' t resemble to those of field outbreaks
生物學特性：雞胚尿囊液經離心、磷鎢酸負染后，電鏡觀察該病毒為典型的冠狀病毒；該毒株的第一代尿囊液對雞胚無肉眼可見的致病作用，當繼代到第5代后，胚體嚴重病變；病毒在雞胚中隨著接種時間的延長，其效價增高， 96h可達到48h的2倍；該毒株可在cef上生長，但不能形成明顯的蝕斑；經1胰酶處理后可凝集雞紅細胞；雞胚的第四代尿囊液病毒回歸動物體，病死雞腎臟呈典型的花斑腎，腺胃則未見肉眼可見的病變。The research on bioconversion of cellulosic materials to produce fuel alcohol around the world mainly focused on the pretreatment of raw materials, the approaches to reduce cellulase cost, the optimization of fermentation course, appropriate use and transformation of microbial species, and the comprehensive use of waste distiller ' s materials etc
各國對纖維質原料生產燃料酒精的研究主要集中在原材料的預處理、如何降低纖維素酶的成本、發酵過程的優化、菌種的合理運用和改造、酒精廢糟的綜合利用等方面。Throughout the culture period, most cells within the colonies continued to be alkaline phosphata se positive and specific stage embryonic antigen ( ssea - 1 ) positive which has been used routinely to characterize embryonic stem and pgcs cells
經過4 7天培養， pgcs形成典型的鳥巢狀集落。集落在傳代過程中保持堿性磷酸酶活性，胚胎階段性特異抗原1 （ ssea - 1 ）免疫熒光染色顯示陽性。Results jiangguiyin capsules can improve activities of glutathion peroxidase and superoxide dismutase ( sod ), accelerate fibrinolysis, while inhibit increasing of malondialdehyde level and platelet aggregation, and slow down thrombosis in ischemic heart muscle
結果薑桂飲可提高缺血區心肌谷胱甘肽過氧化物酶、超氧化物歧化酶活性，抑制丙二醛的含量升高，並可加快纖維蛋白溶解，抑制血小板凝集，延緩血栓形成。The results of lauryl sodium sulfate - polyacrylamide gel electrophoreses ( sds - page ) of the aggregate precipitate and supernatant and the result of high - performance size - exclusion chromatography of the supernatant indicated that, by wrongly linked intermolecular disulfide bonds soluble bi - molecular and tri - molecular egg white lysozyme aggregate could be simultaneously formed except being renatured to native and active egg white lysozymes during the refolding procedure of denatured - reduced egg white lysozyme ; the aggregate precipitate could be further formed by the non - covalent bonds interaction between the soluble hi - molecular egg white lysozyme aggregates, and the soluble tri - molecular egg white lysozyme aggregate could still stay at the supernatant
沉澱和上清液的不連續十二烷基硫酸鈉聚丙烯酰胺凝膠電泳（ sds - page ）和高效凝膠排阻層析分析結果表明，還原脲變性蛋白溶菌酶在稀釋復性過程中除了能夠復性成天然態蛋白溶菌酶分子外，還會形成可溶的蛋白溶菌酶分子二聚體和三聚體，二聚體和三聚體主要是靠分子間二硫鍵的錯配連接而成的；可溶的蛋白溶菌酶分子二聚體之間通過非共價鍵相互作用而形成集聚體沉澱，而可溶的三聚體溶菌酶分子則仍處于復性液上清液中。The reproductive organ blister measles therefore recur, is because blister measles virus deep hiding in ganglion " the establishment gram kj medicinal preparation " series medicineis one kind of structure medicine, it ordinary disease - resistant poisonous medicine composition member is younger than several hundred times, can seep the nerve and the ganglion from the extroversion which suffers injury, is same along with it to sponge absoring water, layer upon layer strips the adsorption in the ganglion the crazy duplication viral body, the destruction virus s nucleotide duplication enzyme, causes it to be separated from the nerve is separated from the virus can massive gathering in the reproductive organ hypodermic, by now again coordinated the establishment gram venereal diseases kj medicinal preparation formidable anti - virus function, comprehensively struck kills the virus, caused the virus not to hide the place, thus achieved thoroughly permanently cured goal
安立克kj劑"系列藥物是一種微分子結構的藥物，它比普通抗病毒藥物的組成分子小幾百倍，能夠從外向內滲透進受損的神經和神經節，隨之就向海綿吸水一樣，層層剝離吸附在神經節里瘋狂復制的病毒體，破壞病毒的核苷酸復制酶，使其脫離神經.脫離出來的病毒會大量的聚集在生殖器皮下，這時再配合安立克性病kj劑強大的抗病毒作用，全面擊殺病毒，使病毒無藏身之地，從而達到徹底根治的目的In this thesis, a strain of associative nitrogen fixing bacteria with high nitrogenase activity was selected and the further studies for it were conducted, the results obtained are as follows : selection and identification of associative nitrogen fixing bacteria with high nitrogenase activity. the nitrogenase activities detected by means of the acetylene reduction method and growth rates tested by optical density of germ suspension under the x 400nm ( od4oo ) of all the strains tested except for the strain w12 reduced gradually due to frequent subculture during a year. however, the strain w12 has been showing high and stable nitrogenase activity and growth rate since it was isolated five years ago
固氮菌株的篩選與鑒定：測定從不同來源分離、收集和保藏的12個固氮菌株或分離物在不同保存時期的固氮酶活性和生長能力，發現多數菌株在初分離得到時固氮酶活性較高，但隨著轉接次數的增多卻逐漸喪失固氮酶活性，生長也隨之減弱，只有w12在五年前從埃及分離得到至今一直保持旺盛生長勢和較高的固氮酶活性， 48h內乙烯生成量可達1800nmol乙烯ml菌液以上，遠遠高於其它菌株，其生長量（ od _ （ 400 ）值）也遠遠高於其它菌株。A novel aqueous two - phase system can be formed by the mixtures of a polymer and cationicanionic surfactants. such a system can be used as a partitioning system of proteins. in this work, we investigated the formation, phase behavior and protein partitioning in aqueous two - phase systems formed by dodecyltriethylammonium bromide / sodium dodecylsulfate / peg and dodecyltriethylammonium bromide / sodium dodecylsulfate / dextran. the ligands with affinity were attached to the polymers and the affinity partitioning of proteins was investigated. it was shown that the surfactants and polymers are enriched in different phases of aqueous two - phase systems. phase separation are promoted by increasing temperature and adding inorganic salts. different proteins are partitioned in different phases. the selectivity of protein partitioning is increased by adding ligands with affinity
報道了由正負離子表面活性劑與高聚物混合溶液形成的一種可用於蛋白質的分離及分析的新型雙水相萃取體系.研究了正負離子表面活性劑（溴化十二烷基三乙銨/十二烷基硫酸鈉）分別與葡聚糖和聚乙二醇混合雙水相體系的形成規律、相行為及牛血清蛋白和溶菌酶在雙水相體系中的分配.通過在高聚物分子中接上親和配基，研究蛋白質在雙水相體系中的親和分配.結果表明，在該體系中，表面活性劑與高聚物分別富集於不同相中.升高溫度及加入無機鹽均可促進雙水相體系的形成，不同蛋白質可分配于不同的相中.親和配基的引入極大地增強了蛋白質分配的選擇性Anti - p21 mouse monoclonal antibody from beijing zhongshan biotechnology anti - mouse or anti - rabbit igg secondary antibody from santa cruz biotechnology ly294002 from sigma biotechnology tritonx - 100 from boehringer mannhein gmbh fluorescein isothiocyanate ( fitc ) conjugated anti - mouse igg antibody was purchased from beijing zhongshan biotechnology hepes from e. metck darmstadt methods superovulation and collection of eggs for superovulation, female kunming mice 4 - 5 week old were injected with pregnant mare serum gonadotropin ( pmsg ), and after 46 - 48 hours with human chorionic ginadotropin. ( hcg ). one - cell fertilized eggs were collected on the next day from oviduct of females
取4一5周齡成熟雌性昆明系小白鼠，腹腔注射pmsg （孕馬血清促性腺激素） 10iu ， 46一48小時后腹腔注射hcg （人絨毛膜促性腺激素） 1oiu ，將注射hcg后的雌鼠與8周以上的成熟雄鼠合籠交配，次日檢察陰栓，將查到陰栓的雌鼠處死，取輸卵管于mz培養液中，解剖鏡下撕開壺腹，釋放細胞團，然後用300林歲nil透明質酸酶消化去除顆粒細胞，口控吸管將卵細胞在m :中反復清洗，然後置於孵箱中，根據時間點收集g2期細胞。The market occupation of retapase project has been over appraised and the market orientation is based on self - reference while less weight has been put on market survey ; the project fund comes inadequately from self - raised resources or by transferring the techniques. in the organization framework of the project, the conventional linear system is adopted, which inhibit imbibing the wisdom of majority ; on personnel management, the r & d professionals have been weighed much more against the management professionals and little effort has been put on building the professionals reservoir, while the personnel incentive tactics are not conducive to the long benefit of the project in addition, the cost management is maldeveloped and the project progress controlling has been neglected
瑞特普酶項目屬生物制藥項目，本文對fdzj公司瑞特普酶項目的管理現狀進行了詳細分析，具體有待改進地方有：市場份額估計過高，市場定位本位化，市場調查注重不足；項目融資僅採用自籌資金及科技成果轉讓的方式；項目組織結構為傳統的直線制，不利於集思廣義，人員配備重研發人才，輕管理人才，人才儲備工作欠缺，人員激勵策略與項目長遠利益不銜接；成本管理欠缺，忽視進度管理等。L. a total of 312 fetuses were collected from 27 wuzhishan inbreeding pigs ( wzsp ) and white minitype pigs to study the factors influencing the establishment of porcine embryonic germ cells, and we draw the following conclusions : ( 1 ) we can get embryonic fibroblasts from d26 - 28 fetuses ( day of first mating is day 0, that is d 0 ) ; in this thesis, sto and mef1 were inactivated by incubation in medium containing 10 g / ml of mitomycin c for 1. 75h ~ 2h ; while pef were inactivated by the same medium for 2. 25h ~ 2. 5h
本實驗以中國近交系五指山小型豬（ wzsp ）和微型白豬為材料，先後從27頭豬採集了312枚早期胎兒用於進行豬胚胎幹細胞（ eg ）建系影響因素研究，得出如下結論： （ 1 ） d26 - 28豬胎兒可用於製作胎兒成纖維細胞。製作飼養層時， sto 、 mef ~ 1細胞用10 g ml絲裂酶素c處理1 . 75h 2h ； pef細胞用絲裂酶素c處理2 . 25h 2 . 5h 。The blastocyst was obtain from the mouse after 3. 5 days of pregnancy, and was cultivated on the mouse embryonic flbroblast feeder layer. the blastocyst usually attach to the feeder layer after 48 hours, then the inner cell mass began to grow and form a big cell mass of embryonic stem cells. these cells can form cell clones with the conformation of embryonic stem cell
囊胚一般在48小時后貼壁並脫去透明帶，由囊胚中的內細胞團增殖形成一個胚胎幹細胞球，待其生長至一定程度后對其進行酶解傳代，在傳代后的細胞中重新生成es集落形態的細胞集落，在集落生長至一定程度后再進行傳代，此後大約每5 ? 7天傳代一次。Escs can be induced into neuron - like cells by sequential neural induction the undifferentiated escs grew in colonies, with clear bound. alkaline phosphatase staining showed dark brown positive particles were distributed in every esc colony. after the sequential neural induction, the cells converted into neuron - like cells, with homogeneous forms, which had round bright cell bodies and thin long bipolar or multipolar processes
5誘導分化前後細胞端粒酶活性檢測（ trap ）結果1 .使用序貫誘導法可將escs大比例誘導分化為神經元樣細胞未分化的escs聚集成團狀生長，細胞排列緊密，集落邊界清楚，堿性磷酸酶染色呈強陽性，胞漿布滿棕黑色顆粒。分享友人