集落形成測定 的英文怎麼說

中文拼音 [jílàxíngchéngcèdìng]
集落形成測定 英文
colony forming assay
  • 集 : gatherassemblecollect
  • 落 : 落名詞[方言] （北方對蓮花落的俗稱） a kind of folk song
  • 成 : Ⅰ動詞1 （完成; 成功） accomplish; succeed 2 （成為; 變為） become; turn into 3 （成全） help comp...
  • 測 : 動詞1. （測量） survey; fathom; measure 2. （測度; 推測） conjecture; infer
  • 定 : Ⅰ形容詞1 （平靜; 穩定） calm; stable 2 （已經確定的; 不改變的） fixed; settled; established Ⅱ動詞...
  • 測定 : determine; determination; setting-out; admeasurement; assignment; assay; finding
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. The author investigated the community of grasshoppers in changbai mountain area during the period of july to september 2001, complementally collected specimen and systematically collected and measured environmental factors from july to october in the following year, and consulted to specimen collected by fengling zhang and yanlong - yang in 1979, 1980 and 1981. by analyzing and classifying the data of five years, the author analyzed the community construction and ecological adaptability of the grasshoppers in the area. the main aspects dealing with the grasshoppers are as follows : ( 1 ) the community construction and faunal geographical elements ; ( 2 ) characteristics of their ecological distribution ; ( 3 ) vertical distribution of grasshoppers in the northern slop ; ( 4 ) the relationship between environmental factors and differences in shape of grasshoppers in different vertical belt of northern slop, etc. in terms of faunal geographical elements of grasshopper communities, 48 species of grasshopper were recorded, belonging to 31 genera, 7 families

    本文作者於2001年7 9月間系統地調查了長白山地區的蝗蟲，並於2002年的7月到10月間對標本進行了補充採集，並對環境因子數據進行了系統的測量和收集，同時參考張鳳嶺、楊彥龍先生1979 、 1980和1981三年採集的標本，通過對標本的鑒定和整理，採用五年的野外工作數據，對長白山地區蝗蟲群落結構及生態適應特性進行了研究，主要研究內容包括：蝗蟲的群落結構及區系地理成分；蝗蟲生態分佈特點；保護區北坡蝗蟲垂直分佈及不同垂直帶內蝗蟲的形態差異和環境因子之間的關系等方面內容。
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