雌雄同序的 的英文怎麼說
中文拼音 [cíxióngtóngxùde]
雌雄同序的
英文
androgynal-
Using a pair of degenerate primers based on the conservative region, hmg - box, of human sry gene, tow different fragments of sox gene, essox3 and essox22 were amplified from female and male eriocheir sinensis, the sequence results indicated that essox3 and essox22 shown high homology to human sox genes, and the identities to human sox genes in dna sequence and amino acid sequence are 84 % 、 85 % and 97 % 、 81 %, respectively. it might be concluded that sox gene was highly conservative in phylogenesis
二、研究論文1 、參照人sry基因hmg - box保守區的序列,設計一對兼并引物, pcr擴增了中華絨螯蟹的sox基因,並對擴增產物進行了克隆和測序。結果在雌雄個體中篩選出兩個不同的sox基因essox3和essox22 ,其dna序列和編碼的氨基酸序列與人相應sox基因的相似性分別為84 % 、 85 %和97 % 、 81 % ,顯示該基因在進化上具高度的保守性。Part two : studies ; l. the sox gene of dinodon refozonatum was amplified by using a pair of primers which can amplify the conservative motif ( hmg - box ) of human sry gene. the amplification band was observed in both male and female dinodon refozonatum, whose length was consistent with that of human sry gene, which about 220bp. the result of sscp analysis showed that there were many differences in the sox gene sequence between dinodon refozonatum and human, and there was a few differences between male and female dinodon refozonatum. 2. using a pair of degenerate primers based on the conservative region, hmg - box, of human sry gene, six different fragments were amplified from either female or male dinodon refozonatum, then cloned by using pmd18 - t vector and sequenced
結果顯示: ( 1 )赤鏈蛇基因的擴增片段與人sry基因擴增片斷大小相同,為220bp左右; ( 2 )雌、雄赤鏈蛇sox基因的擴增片段大小雖然與人的相同,但其單鏈遷移率與人的有較大差異,而且雌雄個體間有明顯差異,預示該基因的dna序列在雌雄個體中可能有差異; 2 、參照人sry基因hmg - box保守區的序列,又設計一對兼并引物,擴增了赤鏈蛇的sox基因,並對擴增產物進行了克隆和測序。
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