雜化酶 的英文怎麼說
中文拼音 [záhuà]
雜化酶
英文
hybrid enzyme-
An analysis of peroxidase and esterase isozyme derived from a generic hybridization among upland rice, barnyard grass and sorghum
高粱三屬雜交過氧化物酶和酯酶同工酶分析Plant biochemists in search of complex enzymatic systems, as a rule use a domesticated plant that is edible !
植物生物化學家在研究復雜的酶系時,通常用一種可食用的栽培植物。The results were as follows : leaching solution by hot water extraction was date in color and had mellow date aroma and the highest fusel oil content but strong bitter taste ; leaching solution by pectinase enzymolysis had the highest reducing sugar content easy for fermentation but excessively high methanol content in fermenting wine ; leaching solution by microwave extraction had the shortest extraction time and fermentation time and the highest ethyl acetate content in wine and the produced wine had special aroma
結果表明, 90熱水浸提,浸提液發酵酒顏色呈棗紅色,雜油醇含量最高,有濃郁棗香,但苦味重;果膠酶酶解浸提,浸提液還原糖含量最高,利於發酵,但發酵酒的甲醇含量過高;微波強化浸提,浸提時間和發酵時間最短,所得棗酒的乙酸乙酯含量最高,且有特殊香味。The purification and assay methods for pectic enzymes are differed with various strains
由於不同菌種產生的果膠酶成分復雜程度不同,分離純化手段和分析方法也不相同。The dopping of clo4 - had a great effect on the sensitivity of the sensor. the glucose oxidase could be immobilized in the polypyrrole membrane when the pyrrole is polymerizing on the electrode
當聚吡咯在電極上氧化聚合時,會形成帶正電荷的聚吡咯骨架,帶負電荷的酶就會通過電荷吸引摻雜到骨架中,從而得到固定。Amperometric nitrate reductase biosensors based on organic - inorganic hybrid material
無機雜化材料的硝酸還原酶電極的研究Northern blot results show that nos. 66 - 1, 84, 89 - 1, 97, 108, 152, 175 and 233 have stronger signal in sp6 - tester than in sp6 - driver ; and no. 23 has weak signal only in sp6 - tester, nos. 94, 165, 172, 185 and 191 have similar hybridization signals in both sp6 - tester and sp6 - driver ; nos. 4, 17, 18, 28, 6 9, 101, 156 - 1, 157 - 1 and 183 do not reveal hybridization signals in both sp6 - tester and sp6 - driver ; the results of sequencing and blastn and blastx on ncbi indicate that no. 23 cdna ( 846bp ) has significant alignments with nicotiana tabacum mrna for elicitor inducible beta - 1 - glucanase nt - sube76, and arabidopsis thaliana clone 7119 for glycosyl hydrolase family 17 ( protein id : at5g55180. 1, supported by cdna : 7119, supported by cdna : gi _ l 87001 54 ) and arabidopsis thaliana beta - 1 - glucanase - like protein ( gi _ 2 1594590 ) ; no. 84 cdna ( 560bp ) has significant alignment with lotus corniculatus aspartate aminotransferase mrna ( complete cds length = 1685, gi | 2605931 | gb | af029898. 1 | af029898 ) for aspartate aminotransferase ; no. 89 - 1 cdna has significant alignment with arabidopsis tha
與同源性最高的擬南芥類似晚期胚胎發生高豐度蛋白比較,二者都具有lea 2結構域、保守分泌蛋白cog5608結構域和低復雜度區,都具有pkc磷酸化位點、酪蛋白激酶磷酸化位點、 n十四酞化位點和酚胺化位點,所不同的是: ( )在結構功能域上, 152全長cdna編碼的蛋白質序列中多了1個lea 2結構域、 l個保守分泌蛋白cog5608結構域和1個低復雜度區; ( 2 )在功能位點上, 152全長cdna編碼的蛋白質具有酪氨酸硫酸化位點、多了l個酪氨酸激酶磷酸化位點和1個可能的天冬氨酸富集區,但沒有n糖基化位點; ( 3 )擬南芥類似晚期胚胎發生高豐度蛋白的lea 2結構域具有顯著性( eBased on the previous studies, the research in this paper was carried out, mainly including two parts as follows : ( 1 ) anammox bacteria and aerobic ammonia oxidizers were detected in situ in 6 sediment samples taken from jiangsu province. molecular techniques, such as fish, pcr, dna cloning and sequencing etc. were used for this purpose. ( 2 ) the continuous cultivation of anammox bacteria from sediment samples were studied, which provides experimental basis for the bioaugamentation of eutrophicated sediment applying anammox process
本論文在前人研究的基礎上,開展了以下兩個方面的工作: ( 1 )採用分子生物學技術熒光原位雜交( fish ) 、多聚酶鏈式反應( pcr ) 、 dna克隆和測序等對采自江蘇省蘇州市、東太湖、新沂河等6個底質樣品進行了厭氧氨氧化菌和傳統氨氧化菌的原位檢測; ( 2 )探討了以底質作為接種體進行厭氧氨氧化菌富集培養的可行性,為天然底質環境中厭氧氨氧化過程的強化,富營養化底質微生物修復的可行性提供一定的依據。Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ,反轉錄成cdna ;利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因,並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因;將mg _ 7單鏈抗體基因插入pcantab5e ;將連接產物轉化感受態tg1大腸桿菌,制備細菌形式的mg _ 7重組噬菌體抗體庫;通過菌落計數和限制性酶切分析( ecor和hind )評估mg _ 7重組噬菌體抗體庫的容量和重組率。These interactions are primarily dependent upon the coordinated actions of ovarian progesterone and estrogen, moreover, many other factors, such as growth factors, cytokines, ecm, adhension molecules, oligosaccharides and proteases, regarded as local mediators, endometrium and embryo have also expressed some specific receptors, via intracellular signal transduction chains and express some key genes, making receptivity of the uterus and synchronized development of the embryo to the blastocyst stage
成功的植入是處于接受態的子宮內膜和具有侵入性的胚胎間的同步協調反應。植入過程受多種生長因子及其受體、細胞因子、粘附分子、蛋白水解酶、寡糖等的精細調控,通過胞內信號轉導及關鍵基因的表達使子宮內膜發生一系列復雜變化。The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii. two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium. the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome. at the same time ; compared agrabactenum - mediated method with gene gun method, the transformation frequency of the former was 16. 7 %, while the latter was 50 %, so gene gun transformation method was suitable for long ya liiliwn
用攜帶有幾丁質酶基因和- 1 、 3葡聚糖酶基因的工程菌,通過農桿菌介導法和基因槍轉化法轉化龍牙百合,經pcr和點雜交檢測證明外源基因已經整合到植物染色體中。同時對農桿菌介導法和基因槍法進行比較,發現農桿菌介導法的轉化率為16 . 7 ,基因槍法的轉化率為50 ,因此可能基因槍轉化法更適于龍牙百合的遺傳轉化。Carville dg, dirmtrijevic n, walsh m, et al. thrombus precursor protein : marker of thrombosis early in the pathogenesis of myocardial infarction [ j ]. clin chem, 1996, 42 ( 9 ) : 1537
諸俊,何曉東,葉書來,等.急性心肌梗死與不穩定心絞痛患者血栓前體蛋白和肌酸激酶的檢測[ j ] .中國動脈硬化雜志, 2002 , 10 ( 2 ) : 165 - 166Although this method gives high sensitivity, the radioactive labels present many problems such as a potential hazard to analyst and environment, which limited its application in dna diagnostic laboratories. in order to overcome these problems a serious of non - radioactive dna probes such as fluorescent, chemiluminescent and electrochemical probes have been developed. although these new methods display many advantages, they have not been used to take place completely the traditional method because of low sensitivity or complex equipment or other shortcomings
自20世紀80年代以來,各種非同位素如酶、熒光素、生物素、地高辛標記的化學發光法和熒光分析法以及以電活性物質做標記的電化學方法相繼問世,這些方法雖然在一定程度上克服了同位素標記的缺陷,但由於存在靈敏度不夠高或檢測系統龐雜或儀器價格昂貴或標記物不穩定等缺陷,還不能完全取代傳統方法。The development of research on synthesis of butyl butyrate and the catalysis of sulfonic acid, inorganic salt, solid superacid, heteropoly acid, enzyme catalysis compound are summarized
摘要綜述了磺酸、無機鹽、固體超強酸、雜多酸、酶催化體系等對合成丁酸丁酯的催化作用及研究進展。Martens p, raabe a, johnsson p. serum s ? 100 and neuron ? specific enolase for predictor of regaining consciousness after global cerebral ischemia j. stroke, 1998, 29 : 2363
何婭,張桂蓮,蘇清華,等.腦卒中患者血清神經元特異性烯醇化酶動態變化及其臨床意義j .臨床神經病學雜志, 2003 , 16 : 143The preparation of functionalized mesoporous molecular sieves were introduced, which are including the formation of the acid active sites, redox active sites and used the support for immsobilizing enzyme catalysts by heteroatomic substitution ; the formation of polymerization active sites, acid active sites or chiral catalysis sites by the organic - inorganic graft ( or hybridization ) ; as the carriers, the transition metals, transition metal oxides and acid catalysts are supported
摘要介紹了介孔分子篩經雜原子取代,引入酸功能、氧化還原功能;經有機無機嫁接(雜合) ,引入聚合催化功能、酸催化功能、手性催化功能;經修飾的介孔分子篩,用作固定化酶催化劑的載體;作為催化劑的載體,用於負載過渡金屬及其氧化物和制備負載化的固體酸催化劑。In the first part of the present work, the expression changes of angiotensinogen ( agt ) and angiotensin - converting enzyme ( ace ) as well as its time course characteristics were investigated by rt - pcr, in situ hybridization and western blotting. we also prepared the polyclonal antiserum against agt for the following work
故本工作應用rt - pcr和原位雜交、 western印跡分析等方法對模擬失重大鼠不同部位動脈血管血管緊張素原( angiotensinogen , agt )及血管緊張素轉化酶( angiotensin - convertingenzyme , ace )基因表達變化的時程特徵進行了觀察,並為后續工作制備了抗agt多克隆抗血清。An amine oxidase - modified polyaniline electrode was formed by immobilization of amine oxidase in polyaniline film by electrochemical doping method
採用電化學摻雜方法首次將胺氧化酶固定在聚苯胺膜中製成聚苯胺用安氧化酶電極。As heterologous probe and subsequently show to code for desired enzymatic activity. after a serial of subcloning coupled with southern hybridization and enzymatic activity assay, the functional s. griseus atcc14811 cholesterol oxidase gene ( chog ) was localized onto 2. 3kb ecori - sall fragment
對phz1140和phz1141進行bamh及bgl的酶譜分析及與choa探針的雜交,將膽同醇氧化酶基因初步定位在8 . 8kbbamh和9 . 9kbbgl片段上。Air of this advanced development provides efficient methods for protein engineering. in this study, a new two - step procedure has been developed for the detection of cholesterol oxidase activity in streptomyces strains. streptomyces griseus atcc14811 was confirmed to produce cholesterol oxidase and it also showed homologies with the cholesterol oxidase gene ( choa ) from streptomyces sp
本實驗設計了簡單易行的鏈黴菌膽固醇氧化酶活性測定指示平板,結合用鏈黴菌sa - coo ( streptomycessp . strainsa - coo )膽固醇氧化酶基因( choa )為異源探針的southern雜交,確證了灰色鏈黴菌atcc14811 ( streptomycesgriseusatcc14811 )具備產生膽固醇氧化酶的能力。分享友人