雞胚細胞 的英文怎麼說

中文拼音 [jīpēixìbāo]
雞胚細胞 英文
chick-embryo cell
  • 雞 : （家禽的一種, 也叫家雞） chicken
  • 胚 : 名詞[生物學] （初期發育的生物體） embryo
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. From dead chickens, one virrus was isolated by using eggs and chicken embryo fibroblast. lt was able to agglutinate chicken ' s erythrocytes and this heamagglutination could be inhibited by newcastle disease antiserum. this strain ' s biological property was tested by barren spot, cross - enutralization and cross - heamagglution inhibited and it was found that it was homological with the standard newcastle disease virus ( ndv ) virulent strain and avirulent strain but it had some diference with the standard strain

    本實驗採用spf雞胚及雞胚原代成纖維細胞，從河北省某雞場新城疫免疫抗體很高的病死雞的腦組織中分離得到一株病毒。此株病毒能凝集雞的紅細胞，並且這種凝集可以被特異性抗血清所抑制。
  3. Like many embryonic cells, myoblasts from prenatal rats or chicks are easy to culture.

    正如許多胚胎細胞一樣，胚胎大鼠或雞的成肌細胞是易於培養的。
  4. Cultivation of avian encephalomyelitis virus in cef

    利用雞胚成纖維細胞培養禽腦脊髓炎病毒的研究
  5. Differentiation of chicken primordial germ cells in vitro

    雞胚原始生殖細胞體外的分化
  6. The results of biological tests have demonstrated that allantoic fluid of the first passage virus did n ' t produce macroscopic pathogenic role to chicken embryos and after passaged for four times, gross lesions were observed in chicken embryo. the virus showed typical coronavirus under electron - microscope and it could n ' t form plaque in cef cells and could hemagglutinates chicken red blood cells after treatment with 1 % trypsin. to surprise, the virus replicated in cef cells also showed hemagglutination activity to chicken red blood cells. in addition, the spf chickens which inoculated with the virus isolated from the chicken damaged tissue showed clinical sign and grow lesion, but it ' s gross lesion did n ' t resemble to those of field outbreaks

    生物學特性：雞胚尿囊液經離心、磷鎢酸負染后，電鏡觀察該病毒為典型的冠狀病毒；該毒株的第一代尿囊液對雞胚無肉眼可見的致病作用，當繼代到第5代后，胚體嚴重病變；病毒在雞胚中隨著接種時間的延長，其效價增高， 96h可達到48h的2倍；該毒株可在cef上生長，但不能形成明顯的蝕斑；經1胰酶處理后可凝集雞紅細胞；雞胚的第四代尿囊液病毒回歸動物體，病死雞腎臟呈典型的花斑腎，腺胃則未見肉眼可見的病變。
  7. Development of chicken embryo fibroblast cell line resistant to j subgroup avian leukosis virus infection

    亞群禽白血病病毒的雞胚成纖維細胞系建立
  8. The steady dead generation and time that was caused by the isolated virus was certain by chicken embryo which was inoculated on seven or nine days. the histopathological changs of the infectious stunting syndrom were studied by the way of ordinary paraffin section and he dying. the experimental result were as follows : the test proved that the changes of the chicken embryo were different in different stage. the chicken embryo dead in a week after it inoculated. the body was dropsy and hemorrhage. dead before it hatched out, the embyo body were dropsy, pale and slime. the liver was yellow and swolled, gallbladder ( vesica fellea ) was filled with bile. bursa and glandula thymus analosis. the kindey dropsy. bowel lamina were humble, dilatation. gas and yellow foam were filled the bowel. histopathological changes were that, in early stage, obvious changes of liver and kindey were dropsy, hemorrhage and necrosis. two types eosinophilic intranuclear inclusion bodies including large round and little granular were present in cells of the above organs. the obvious changes of bursa were dropsy, adverse folliiculated growth and little lymphocytes proliferating, 19 - 21 days chicken embryo, one or two big empty vacuoles were prensent in cells of liver and kindey. the number of the folliculi was growing, the vacuoles between cells were larger

    膽囊充盈、其內充滿稀薄的膽汁；法氏囊、胸腺萎縮，腸道擴張、腸壁菲薄、內充滿氣體及黃色泡沫狀物；腎臟腫大。病理組織學變化方面，早期肝臟、腎臟、腸主要以出血、水腫和壞死為主，且肝細胞核及腎小管的上皮細胞核內均發現有核內包涵體，包涵體呈嗜酸性，為大型圓形包涵體或不規則的顆粒狀；法氏囊則以水腫、濾泡發育不良、小型淋巴細胞數量增多為主。 19 21日齡雞胚肝細胞、腎小管上皮細胞的胞漿內出現1 2各大的空泡，法氏囊濾泡數目增多細胞間有較大空隙。
  9. Spf chickens with 21 - day - old were infected subcutaneously with oil - emulsion vaccine of ibdv of germinal or cellcular and injected intramuscularly with different dosages bursin which gain through ultrafilter. lt is proved that bursin of chickens and ducks can both shorten the time of antibody induced against ibdv, raise the level of serum antibody. they make chickens obtaining strong immunocompetente in a short time. agp liters of the group of infecting 0. 4mlcbs + ibdv of germinal and 0. 8mlcbs + ibdv of cellcular or 0. 8mldbs + ibdv of germinal and cellcular are higher than immune control group about 2 liters averagely. the chickens were inoculated with ibdv live vaccine mixed with the different dosages of lyophilized bursin by the eye drop method. the results sugest that cbs or dbs of different dosages can both improve the antibody inducation to different age chickens against ibdv. they may also alleviate the immunological injury of activated virus to bursa of fabricius. and promote the repairation of the lesion. it can be found that bs can raise body weight gain and feed coversion ratio

    將超濾獲得的法氏囊活性肽分別以不同劑量肌肉注射21日齡spf雞，同時頸部皮下注射ibd胚毒或細胞毒滅活苗，結果表明：雞、鴨法氏囊活性肽都能夠縮短ibd油苗誘導產生抗體的時間，提高抗體水平，使雞可以在比較短的時間內獲得堅強的免疫。 0 . 4mlcbs胚毒組和0 . 8mlcbs細胞毒組或0 . 8mldbs胚毒和細胞毒組的agp抗體滴度平均比免疫對照組高2個滴度。將法氏囊活性肽與ibd活苗聯合免疫雞，結果表明：不同劑量的cbs和dbs都可以對不同日齡雞ibd抗體的產生有不同程度的促進作用；還可以減少弱毒對雞法氏囊組織的損傷，加快其修復。
  10. Ganoderma lucidum polysaccharides inhibit angiogenesis and cell adhesion in chicken chorioallantoic membrane model

    靈芝多糖抑制雞胚尿囊膜模型中的血管生成及細胞黏附
  11. Culture of the attenuated a66 strain of duck hepatitis virus in chicken embryo fibroblast

    66弱毒株在雞胚成纖維細胞上的培養
  12. Effect of removal of external calcium on phosphoinositide hydrolysis in cultured myotubes of embry - onic chicken

    去細胞外鈣對培養雞胚肌管磷脂酰肌醇水解的影響英文
  13. Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )

    利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠，然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞（ cef ）做免疫熒光試驗（ fa ） ，進行其分泌抗meq單克隆抗體（ mcab ）能力的篩選。
  14. In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv

    本研究採用脂質體轉染方法，將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞，經6輪蝕斑克隆、篩選、表達， pcr鑒定和dot - elisa檢測，證明該重組病毒已構建成功，並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。
  15. Pre - clinical studies showed that needleless injected fluids follow the path of least resistance and does not pass into the substance of bone, media of blood vessels or into the fibre substance of the nerve trunks

    臨床研究顯示，無針美槊療法所注入的液體不會滲入骨頭、血管或神經內，它會沿著最少抗體的路徑進入肌膚組織，並且加速皮膚細胞的新陳代謝，讓雞尾酒中胚層綜合物質更容易於肌膚所吸收。
  16. In young chickens aev induces paralysis, ataxia and muscular dystrophy, while in older chickens, infection is usually subclinical, resulting in a decline in egg production and hatchability. infectivity was shown to remain unaffected by chloroform, low ph, pepsin, trypsin and deoxyribonuclease. magnesium cations were shown to stabilise preparations of the virus against heat inactivation. the buoyant density of virions are 1. 31g / ml. the diameter of the virion was estimated to be 22 to 30nm. the aev can be adapted to grow in chicken embryo. the inability of aev to grow effeciently in most cell cultures

    幼雞感染該病毒后，引起麻痹、頭頸震顫甚至共濟失調，而成雞常呈亞臨床感染或導致產蛋量和孵化率下降。病毒的感染性不受氯仿、低ph 、胃蛋白酶、胰酶和脫氧核糖核酸酶的影響，鎂離子可增強病毒對熱的穩定性，病毒的浮密度為1 . 31g ml ，直徑為22 - 30nm ，該病毒主要在雞胚中增殖，在大多數細胞培養物中不生長。
  17. At primary culture, pgcs co - cultured with their gonadal stromall cells were well grown. when subculture, we used primary chicken embryonic fibroblast ( pcef ), primary mice embryonic fibroblast ( pmef ) and snl cells to make feeder cells. forward research founded that the pcef cells were the most suitable for the growth of putative eg cells when having various cytokines

    繼代培養時，經過反復實驗比較雞胚原代成纖維細胞飼養層（ pcef ） 、小鼠原代成纖維細胞飼養層（ pmef ） 、 snl細胞飼養層等三種不同飼養層的作用效果，最終發現以雞胚原代成纖維細胞製作飼養層同時添加各種生長因子最適合雞類eg細胞的生長，但pcef與pmef之間的差異不顯著。
  18. The eg cell culture media consisting of dmem medium supplemented with fbs, chicken serum, beta - mercaptoethanol, l - glutamine. hepes, chicken embryonic extract and cytokines etc. after 24 hours culture, the isolated pgcs were selectively attached on the gonadal stromall cells in the plates

    加入新鮮的eg細胞培養液（含dmem 、胎牛血清、雞血清、 -巰基乙醇、 l -谷氨酰胺、 hepes 、雞胚浸出液以及細胞因子等成分）培養24小時以後， pgcs開始部分貼附於共培養的生殖原基細胞上。
  19. In addition, the bone marrow - deried stem cells, which could be differentiated into neurons and other cells making up the central nerous system, spread far and wide and behaed like neural stem cells when transplanted into the brain tissue of chicken embryos

    除此以外，骨髓源性幹細胞能夠分化為神經元和其它細胞共同構成中樞神經系統，當其植入到小雞胚胎的腦組織中後分布非常廣泛，其特性同神經幹細胞類似。
  20. Chick embryo cell culture

    雞胚細胞培養
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