離子交換純化 的英文怎麼說

中文拼音 [lízijiāohuànchúnhuà]
離子交換純化 英文
ion-exchange purification
  • 離 : Ⅰ動詞1 （離開） leave; part from; be away from; separate 2 （背離） go against 3 （缺少） dispens...
  • 子 : 子Ⅰ名詞1 （兒子） son 2 （人的通稱） person 3 （古代特指有學問的男人） ancient title of respect f...
  • 交 : Ⅰ動詞1 （把事物轉移給有關方面） hand over; give up; deliver 2 （到某一時辰或季節） reach （a cert...
  • 換 : 動詞1. （給人東西同時從他那裡取得別的東西） exchange; barter; trade 2. （變換; 更換） change 3. （兌換） exchange; cash
  • 純 : 形容詞1 （純凈; 不含雜質） pure; unmixed 2 （純粹; 單純） simple; pure and simple 3 （純熟） skil...
  • 離子 : [物理學] ion
  • 純化 : purification; purifying; depuration; edulcoration; purify
  1. Strain bl21, and gene expression was induced by iptg. the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene. using ion exchange chromatography and gel filtration chromatography, the chimera proteins were purified, and both of them showed a single band in tricine - sds - page. the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities, but gain platelet aggregation inhibitory activities as well

    通過離子交換層析和凝膠過濾層分別對兩種嵌合體蛋白進行純化，純化產物在tricine - sds - page中都顯示為單一條帶。活性分析結果表明兩種嵌合體蛋白在保留水蛭素抗凝血酶活力的同時，還呈現抗血小板聚集活性。
  2. 6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page

    將枯草芽孢桿菌超聲波破壁后的粗提取物進行分段鹽析、 deae - sepharose陰離子交換柱層析， blue - sepharosecl - 6b特異結合柱層析和sephadexg - 200凝膠過濾等純化步驟，得到聚丙烯酰胺凝膠電泳為單一蛋白區帶，比活為1 . 46u mg的酶制劑。
  3. This enzyme was different with the ones reported in the past. a phosphatase was isolated from the chloroplast thylakoid membrane of ipomoea aquatica, by nacl extration, ammonium sulfate precipitation, ion - exchange chromatography and hydrophic chromatography through butyl - toyopearl 650m column

    使用nacl抽提、硫酸銨分步沉澱、離子交換和butyl - toyopearl650m疏水柱層析等方法，從蕹菜葉綠體類囊體膜中分離純化到一種蛋白磷酸酯酶。
  4. Based on the extensive studies of subtilisin - like protease ( prl ) of metarhizium anisopliae, extracellullar serine protease is suggested to be a key enzyme involved in the fimgal penetration to invertebrates. the investigation of serine protease in the nematode infected by owvtl may help to understand the mechanism of nematophagous fimgi as biological control agents. a 3l kda serine protease was isolated and purified from the liquid culture of h rhossiliensis owvtl challenged with nematode panagrellus redivivus

    本研究利用線蟲誘導下owvt - 1菌株液體發酵，通過粗分級分離、離子交換層析和凝膠過濾層析分離提純了一個分子量為31kda的絲氨酸蛋白酶，生物學測定表明其對大豆胞囊線蟲二齡幼蟲具有致死作用，同時測定了該酶理化特性，酶活力在75附近酶活力最高，隨著ph的增加酶的穩定性升高，與膽堿酯酶具有相似的ph曲線，對特異性底物aape （ suc - ala - ala - pro - glu - pna ）具有作用， ssi和ci - 2抑制該酶的活性。
  5. The ps ii native fractions ( 20 % and 30 % ) were loaded onto a deae column. the fraction eluted with 150 mm nacl was presented dcip reduction activity and was highly depleted in chi c and xanthophylls, and as such could be considered a ps ii core complex

    對于有dcip光還原活性的20和30層帶的復合物，進一步deae離子交換層析純化。 150mmnacl洗脫純化后的樣品經過熒光激發光譜測定發現，已經去除了葉綠素c和墨角藻黃素，並且仍然具有dcip的光活性，分析是ps核心復合物。
  6. The xerocomus spadiceus lectin, xsl, was isolated from extracts of fruiting bodies of the mushroom xerocomus spadiceus using a procedure that involved ( nh4 ) 2so4 precipitation, anion exchange chromatography on deae - cellulose, affinity chromatography on affi - gel blue gel, cation exchange chromatography on cm - cellulose, and gel filtration by fast protein liquid chromatography on superdex 75

    從磚紅絨蓋牛肝菌（ xerocomusspadiceus ）子實體粗提物中，經過deae -纖維素陰離子交換層析、 affi - gelbluegel親和層析、 cm -纖維素陽離子交換層析和superdex75fplc凝膠過濾，純化了磚紅絨蓋牛肝菌凝集素。
  7. Medium experiments were arranged under uniform design, and then an optimum medium was got accordingly. the culture liquid was centrifugalized at 3, 500r / min for 30min, then ammonium sulfate was added into the supernatant to a final concentration of 30 % to precipitate the others

    通過硫酸銨分級沉澱、 deaesephadexa - 50陰離子交換凝膠層析和sephadexg - 75凝膠柱層析對發酵液進行分離和純化，並得到電泳純的酶。
  8. A neurotoxic peptide ( named huwentoxin - v ) was purified from the venom of the spider by a combination of ion exchange chromatography and reverse phase hplc. hwtx - v has 35 amino acid residues, with the molecular mass 4111. 4da. the amino acid sequence has been determined as nh2 - ecrwylggcsqdgdcckhlqchsn - yewcvwdgtfs - cooh, which consists of 6 cys, formed three pairs of disulfide bridges

    本文報道從虎紋捕鳥蛛（ selenocoimahuwena ）粗毒中，結合陽離子交換和反相高效液相色譜分離純化出一種昆蟲毒素，命名為虎紋捕鳥蛛毒素- （ huwentoxin - ， hwtx - ） ；其天然突變體（ themutantofhuwentoxin ， mhwtx - ）也同時純化出來。
  9. Using i - dopa as a specific substrate, phenoloxidase ( po ) from penaeus chinensis hemolymph was purified by gel - filtration and ion - exchange chromatography, and characterized in terms of its molecular weight and enzymatic properties in this study

    本文以中國對蝦（ penaeuschinensis ）的血淋巴為材料，利用凝膠過濾和離子交換等方法，對酚氧化酶（ phenoloxidase ）進行了分離純化和生物化學性質研究。
  10. Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution

    3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵，用緩沖液抽提后，經硫酸按沉澱， deae一纖維素離子交換層析，聚丙烯酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸酶，用sds一page檢測為一條均一譜帶，其分子量約為78kd 。
  11. Mm ). mg2 +, mn2 +, zn2 +, fe2 +, fe3 +, cu2 + have enabled effect on enzyme activation and edta produce a strong inhibitory effect on the enzyme. embranch amino acid have no effect on the enzyme

    在離子交換柱層析中，採用不同的ph值及不同類型的緩沖液對純化條件進行優化，最終選擇了ph6 . 0mes緩沖液，並得到了酶蛋白洗脫點為0 . 24 0 . 32mol lnacl 。
  12. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  13. Fusion expression of m - centrin in e. coil bl21 was performed by induction of fptg. fusion protein was digested by ppase and was purified by gst chelating affinity chromatography and ion exchange chromatography. the final products were checked by sds - page gel

    融合蛋白gst - m - centrin菌體經過超聲波裂解后得到的上清夜經過gst親和層析後用prescissionprotease （ ppase ）酶切，酶切產物再次經過gst親和層析和hitrapq陰離子交換層析兩步柱層析純化后，得到純度較高的的m - centrin 。
  14. As we know tpk is the important key enzyme in cell growth and metabolism. so what we have done is of great significance to study further the biological functions of the new cloned protein related to bsp proteins

    我們從大鼠子宮中通過deae 52離子交換柱等純化得到tpk樣品，收集有活性部分，一切操作在4t中進行，然後觀察通過基因工程得到的hbrp對tpk活性的影響。
  15. When being incubated at 60, 70, 80, chlase could lose its half activities in 21 minutes, 22 minutes, 18 minutes. with different chl, chlase showed different kinetic properties. different ions were found having different effects on chlase activities

    以菠菜為材料，分離了pao活力檢測所需的還原性劑fd ，經丙酮沉澱、透析脫鹽、 deae離子交換柱層析等純化步驟進行了純化， fd純化了6 . 6倍。
  16. - acetolactate decarboxylase is purifed from cell extract by 50 % - 80 % ammonium sulfate - fractionation, 50, 2min heat treatment and deae - sepharose fast flow column chromatography, which we study the different ph and different buffer of deae - sepharose fast flow column chromatography and conclude ph 6

    對其酶學性質進行了研究。 -乙酰乳酸脫羧酶經50 80硫酸銨分級沉澱、 50 ， 2min熱處理、 deae - sepharosefastflow離子交換柱層析方法分離純化。
  17. - acetolactate decarboxylase are widely found among bacterial strains but not in other groups of organisms. the enzyme has been demonstrated to be effective for removal of acetolactate and widely used in beer product. in this paper, - acetolactate decarboxylase from bacillus subtilis was purifed to homogeneity from cell extract by ammonium sulfate - fractionation, heat treatment, deae - sepharose fast flow column chromatography

    本文對來源於枯草芽孢桿菌（ bacillussubtilis ） 3226 - 5的-乙酰乳酸脫羧酶經硫酸銨分級沉澱、熱處理、 deae - sepharosefastflow離子交換柱層析等分離純化步驟，得到sds - paeg電泳純，通過n末端氨基酸序列分析驗證酶蛋白的純度。
  18. Ion exchange is used extensively for the separation and purification of amino acids

    摘要離子交換法廣泛用於氨基酸的分離純化。
  19. According the key factors we find, we bring forward a new conception : multilevel suppressor and design a new high performance suppressor whose ion - exchange membrane has bigger areas and using three electrodes including one cathode ( anode ) and two anodes ( cathode ), at the same time we fill the suppression compartment with one kind of ion exchange resin which has moderate exchange capacity. according to our experiment ' s results, we find the new type suppressor has quite high working current efficiency and suppressing capacity. in most cases, the suppressor ' s current efficiency is over 90 % ; the suppressor can transform the naoh ( concentration : 200mmol / l, flow rate : i. oml / min, conductance : over 10000 i - i s cm " ) to pure water ( conductance : 8. 9 it s cm in chapter 3, the high performance suppressor is applied in determination some trace - amounts ions in plating solution, sewage. in this chapter, we also have a research on the gradient ion chromatography

    第二章首先以xyz - 1型電化學抑制柱為例，分析了電化學抑制柱的抑制過程得出影響抑制容量的主要因素主要是抑制柱的電流效率和離子交換膜的極限電流密度，因此採用中等交換能力的離子交換樹脂作為抑制室的填料以提高電流效率，在通常情況下電流效率可達到90以上；在選用同種離子交換膜的前提下，可通過增加離子交換膜的有效面積達到提高極限電流的目的從而提高抑制柱的抑制容量，因此提出了多級抑制的概念並據此研製了共電極式高容量電化學抑制柱，該抑制柱最高可將流速為1 . 0ml / min ，濃度為200mmol / l電導率超過10000 s ? cm ~ （ - 1 ）氫氧化鈉溶液抑制為電導率低至8 . 9 s ? cm ~ （ - 1 ）的純水，並且具有穩定性高、分析結果準確等優點。
  20. Mutated plasmid was transformed into e. coli tg1 cells to produce engineered peptide, then the peptide was purified by cm sepharose ion - exchange column. in vitro bactericidal assay and drug withdrawal were used to identify the bioactivity of the engineered peptide. the planar lipid bilayer membrane was used to assay the electrophysiology of the engineered peptide. toxicity studies on mammalian cells were used to assay the toxicity of the engineered peptide

    將重組質粒轉化入大腸桿菌tgi工程菌中，生產構建的工程多膚，離子交換純化后獲得工程多膚初步純化產物，體外抗菌試驗、藥物撤離試驗檢測工程多膚的抗菌活性，在人工脂質膜上測定其形成離子通道的特性以初步研究抗菌機理， ?並觀察其對真核細胞的毒性作用。
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