離心沖洗 的英文怎麼說
中文拼音 [líxīnchōngxǐ]
離心沖洗
英文
centrifugal elutriation-
In order to produce monoclonal antibodies, first, several v. dahliae isolates were grown in liquid czapeak medium, after rinsing mycelia and eliminating zoospores, the fungal tissue was homogenized with the pestle in liquid nitrogen and then transferred to test tubes and was centrifuged in tris - hcl buffer
在制備抗原的過程中,首先液體振蕩培養了若干株棉花黃萎病菌,經過沖洗除孢子、液氮研磨,用tris - hcl抽提,再離心制得菌絲蛋白提取液,可作為電泳樣品。Secondly, put acetone in the abstracting liquor, put it aside for one hour and slowly centrifuge. thirdly the deposit is fully dissolved and heated for 15 minutes in 50 c water, and get crude enzyme solution through centrifuge
分離純化將蘆薈的葉子洗凈、擦乾,加入tris - hcl緩沖液進行組織搗碎提取sod ,高速離心;將提取液用丙酮使sod沉澱、靜止,低速離心;將沉澱用蒸餾水充分溶解, 50水浴中加熱15min ,離心除去部分雜蛋白,獲得粗酶液。
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