離體細胞培養 的英文怎麼說

中文拼音 [lítǐxìbāopéiyǎng]
離體細胞培養 英文
cell culture in vitro
  • 離 : Ⅰ動詞1 （離開） leave; part from; be away from; separate 2 （背離） go against 3 （缺少） dispens...
  • 體 : 體構詞成分。
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  • 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
  1. We hope that our study will provide us with more comprehensive knowledge of the mechanisms of immune regulation and the roles that dc and complement play in innate and acquired immunity, as well as to lay a foundation for further exploration of the roles dc play in antigen - specific immune responses and immune tolerance from a new perspective. part i expression of complement receptors and complement - associated molecules on dendritic cells derived from distinct - origin at different stages of development two subsets of dendritic cells were generated from precursor cells isolated by means of magnetic cell separation system

    曰補體受體及其相關分子在modc上的表達不同分化階段的單核細胞衍生性dc （ monocytes一deriveddc ， modc ）的誘導:將新鮮分離的單核細胞mo ，在含有gm一csf和工l一4培養體系中誘導5一7d ，即分化為未成熟modc ;對培養至sd的未成熟modc ，用tnfa刺激zd ，即分化為成熟modc ;此時再用lps刺激24h ，即為活化的modc 。
  2. Isolation and culture of bovine fetal fibroblasts

    牛胎兒成纖維細胞分離與體外培養
  3. We focused on the following aspects ; 1 ) we first assayed the expression of complement receptors and complement - associated molecules on distinct subsets of dendritic cells during their development in order to understand the physical basis of the sensitivity of dendritic cells to complement and its split products ; we next studied the effects of complement activation on the survival of dendritic cells during their development ; and finally examined the effects of the whole complement system, focusing on the ability of one of the split products of complement activation, c5a, and its first subcomponent - c1q, to influence chemotaxis of dendritic cells, as well as allo - t cell stimulatory activity of dc

    我們通過免疫磁珠分離了兩種人dc前體，即髓系來源的單核細胞（ monocytes ， mo ）和淋巴系來源的漿細胞樣dc （ plasmaeytoiddendriticeells ， pdc ） ，對這兩個不同dc亞群進行體外誘導培養，使其處于不同的分化發育階段，然後檢測了其表達補體受體一cd35 （ cri ） 、 cd21 （ crz ） 、 cdilb （ cr3 ） 、 cdlle （ cr4 ） ，補體調控蛋白一cd46 、 cd55 、以及部分補體片斷分子受體一c3ar 、 csar 、 clqrp的水平。
  4. Other spiroplasmas have been isolated and cultured on cell-free media.

    其它螺原體已被分離出來，並在無細胞的培養上培養。
  5. Floral organs have been successfully induced from the regenerated floral buds of ffyacinthus orienralis l. by precisely controlling exogenous hormones in the medium. under high concentration of cytokinin and auxin, the regenerated floral bud produces only tepals

    以風信子花被片為外植體，通過控制培養基中細胞分裂素和生長素的濃度，可誘導花芽的再生和控制產生花器官的種類，建立起離體花器官定向控制系統。
  6. Eg cells of the 2th and 4th passage were akp ( alkaline phosphate ) positive. when cultured on degenerated feeder layers or in suspension, eg c ells formed embryoid bodies ( ebs ) in vitro

    當eg細胞脫離飼養層懸浮培養，或在衰老的飼養層上延遲培養時，發現eg細胞或單個存在，或聚集成團，形成類似於早期胚胎的囊狀胚體結構
  7. Thus, in order to investigate the developmental pathways not only involved in the regulation of growth and patterning, but also in the determination of cell lineages and differentiation, we utilized the fluorescent immunohistochemical methods, flow cytometry analysis sorting ( facs ) and molecular methods to investigate the developmental law of mammary gland at the different developmental stages, distribution of the stem cells in mammary gland, the methods of isolation, culture and evaluation for the stem cells, the multipotent abilities in vivo and in vitro, and the efficient cultural system for stem cells enriched in vitro. the results showed below : 1

    我們以小鼠為模型，運用組織化學、免疫熒光組織（細胞）化學、流式細胞儀分選方法（ facs ）以及分子生物學手段，研究了小鼠乳腺的發育規律：小鼠乳腺組織中類乳腺幹細胞：小鼠乳腺細胞的分離、培養以及類乳腺幹細胞的鑒定；小鼠類乳腺幹細胞分化的潛能；小鼠乳腺類腺體體外短期培養富集類乳腺幹細胞體系的優化等。研究結果表明： 1
  8. Isolation and long term culture of human primordial germ cells

    人原始生殖細胞的分離和體外培養
  9. Expanded bed adsorption ( eba ) is a novel bioseparation technique, which integrates clarification, concentration and initial purification into a single unit operation. it enables proteins to be recovered directly from unclarified cultivations of microorganisms or cells and homogenates of disrupted cells, without the need for prior removal of suspended solids. matrix is the principal " hardware " pillar supporting the successful application of eba

    擴張床吸附（ eba ）技術是一種新型的生化分離技術，它集成了固液分離、濃縮和初期純化於一步單元操作之中，可以直接從含有細胞和細胞碎片的發酵液或培養液中提取目標蛋白，而不必事先除去懸浮的固體顆粒。
  10. Plantlet regeneration by isolated microspore culture of somatic hybrid of eggplant

    茄子體細胞雜種游離小孢子培養獲得再生植株
  11. The isolation and cufure of the ductal cells of smg in vitro

    實驗結果一、領下腺細胞的分離純化與體外培養、傳代。
  12. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  13. Cotyledon and true leaves of stem mustard were cultured on ms medium supplemented with different concentrations of various cytokinins ( ba, cppu, tdz or kt ) alone or in combination with naa or iba, and the adventitious buds regeneration rate was evaluated.

    以榨菜子葉、真葉為外植體進行離體培養，用ba 、 cppu 、 tdz和kt等細胞分裂素和生長素naa 、 iba組合誘導子葉、真葉再生不定芽試驗。
  14. Primary culture of rat preadipocyte were prepared from the epididymal, inguinal and perirenal the fat pads of male normal, healthy, 15 - 20 days sprague - dawley rats. the preadipocyte grew better under the condition of 37, 95 % humidity, 5 % co2, ph 7. 0 - 7. 2, centrifuged at 1000r / min, m199medium, and 10 % fetal bo vine serum, seeded at a density of 4 l04, 5 l04, / cm2. oil red o staining was the special method to distinguish adipocyte from other cells, gimsa and he could determine the stage of the adiopcyte differentiation through the number of lipid drop, size and the position of the nucleolus of the staining fat cell

    經過多次實驗，確定本實驗室大鼠前體脂肪細胞的最佳培養條件是：溫度為37 ，濕度為95 ， co _ 2濃度為5 ， ph值為7 . 0 7 . 2 ，離心力為1000r / min ，培養基為m _ （ 199 ）培養基，胎牛血清濃度為10 ，合適細胞接種密度為4 10 ~ 4 、 5 10 ~ 4個/ cm ~ 2 ，染色結果表明：油紅o染色是鑒定脂肪細胞的特異方法， gimsa和he染色可根據不同區域染色程度、著色差別判斷細胞核的位置及脂滴大小、多少，觀察大鼠前體脂肪細胞分化過程中的形態變化，進而確定脂肪細胞的分化階段。
  15. Since the success of dolly, the first cloned sheep with the adult somatic cells as karyoplast donor, new approaches have been developed for nuclear transfer technology. here we describe a handmade cloning method which combines the chemical induced enuleation and zona - free technology in embryo culture. enuleated oocytes were derived by exposing the oocytes to demecolcine and cytoheximide supplemented mdium sequently and its chromosome was depleted to the first polar body

    將培養10h的化學去核卵母細胞與供體成纖維細胞融合后lh 、 2h 、 3h ，分別有77 . 6 % 、 70 . 6 % 、 58 . 9 %重構胚的染色質發生凝集，其餘胚胎的染色體則處于原核期;而只在融合后3h ， 27 . 9 %重構胚被標記出組裝的紡錘體，且其中的同源染色體己經分離。
  16. One major obstacle for commercialization is the limited availability of sufficient quantities of defined sponge starting material. in vitro sponge cell cultures appear to be very attractive because they are completely controlled and can easily be manipulated for optimal production of the target metabolites

    為解決在海綿藥物開發過程中的海綿生物量的「供給問題」以及其它研究領域對海綿細胞培養的需求，最近十年來，國際上開始了海綿細胞離體培養的研究。
  17. The aims of this work are to study the mechanisms for cell aggregation, division / propagation, and metabolism in in vitro sponge primmorph culture system ; and to further optimize the primmorph culture toward the establishment of a continuous sponge cell line. primmorph cultures from mcp were established using several marine sponges species, collected from china oceans and mediterranean sea

    本論文圍繞海綿細胞離體穩定培養體系的建立，對海綿細胞離體培養的技術和規律進行系統的研究和優化，提出了一種新的富集海綿中全能細胞原細胞成團培養的技術adcp - primmorph （ primmorphfromarchaeocyte - dominant - cellpopulation ） 。
  18. Dissociation and culture research of porcine donor cell and cell subculture in somatic cell nuclear transfer

    豬核移植供體細胞的分離培養及傳代的研究
  19. All study aim is to lay a foundation for clinic appliance. methods : 1 ) hpfl was isolated by enzymatic digestion derived from rat fetal liver on ed13. 5d. furthermore, erythrocyte and other cell were removed from hpfl by erythrocyte - cracking solution and different attachment method

    研究方法： 1 ）取ed13 . 5d的大鼠胎肝，酶消化法離散細胞，用紅細胞裂解液去除紅細胞，差速貼壁法去除其它細胞，接種于不同的基質和培養液中， mtt法比較不同培養液和培養基質對大鼠hpfl的體外生長影響。
  20. Culture a population of microorganisms or dissociated cells of a tissue grown on or within a solid or liquid medium for experimental purposes

    培養：以試驗為目的在固體或液體培養基上生長的微生物種群或解離的細胞或組織。
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