離體細胞核 的英文怎麼說

中文拼音 [lítǐxìbāohé]
離體細胞核 英文
isolated cell nuclei
  • 離 : Ⅰ動詞1 （離開） leave; part from; be away from; separate 2 （背離） go against 3 （缺少） dispens...
  • 體 : 體構詞成分。
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 核 : 核構詞成分。
  • 細胞核 : nucleus; caryon; cyteblast; cell nucleus
  • 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
  1. We hope that our study will provide us with more comprehensive knowledge of the mechanisms of immune regulation and the roles that dc and complement play in innate and acquired immunity, as well as to lay a foundation for further exploration of the roles dc play in antigen - specific immune responses and immune tolerance from a new perspective. part i expression of complement receptors and complement - associated molecules on dendritic cells derived from distinct - origin at different stages of development two subsets of dendritic cells were generated from precursor cells isolated by means of magnetic cell separation system

    曰補體受體及其相關分子在modc上的表達不同分化階段的單核細胞衍生性dc （ monocytes一deriveddc ， modc ）的誘導:將新鮮分離的單核細胞mo ，在含有gm一csf和工l一4培養體系中誘導5一7d ，即分化為未成熟modc ;對培養至sd的未成熟modc ，用tnfa刺激zd ，即分化為成熟modc ;此時再用lps刺激24h ，即為活化的modc 。
  2. We focused on the following aspects ; 1 ) we first assayed the expression of complement receptors and complement - associated molecules on distinct subsets of dendritic cells during their development in order to understand the physical basis of the sensitivity of dendritic cells to complement and its split products ; we next studied the effects of complement activation on the survival of dendritic cells during their development ; and finally examined the effects of the whole complement system, focusing on the ability of one of the split products of complement activation, c5a, and its first subcomponent - c1q, to influence chemotaxis of dendritic cells, as well as allo - t cell stimulatory activity of dc

    我們通過免疫磁珠分離了兩種人dc前體，即髓系來源的單核細胞（ monocytes ， mo ）和淋巴系來源的漿細胞樣dc （ plasmaeytoiddendriticeells ， pdc ） ，對這兩個不同dc亞群進行體外誘導培養，使其處于不同的分化發育階段，然後檢測了其表達補體受體一cd35 （ cri ） 、 cd21 （ crz ） 、 cdilb （ cr3 ） 、 cdlle （ cr4 ） ，補體調控蛋白一cd46 、 cd55 、以及部分補體片斷分子受體一c3ar 、 csar 、 clqrp的水平。
  3. The ribosomes, made up of rna and protein, are found either lining the endoplasmic reticulum or free within the cell.

    核糖體由蛋白質和RNA組成，有的排列在內質網上，有的則在細胞中呈游離狀態。
  4. Primary culture of rat preadipocyte were prepared from the epididymal, inguinal and perirenal the fat pads of male normal, healthy, 15 - 20 days sprague - dawley rats. the preadipocyte grew better under the condition of 37, 95 % humidity, 5 % co2, ph 7. 0 - 7. 2, centrifuged at 1000r / min, m199medium, and 10 % fetal bo vine serum, seeded at a density of 4 l04, 5 l04, / cm2. oil red o staining was the special method to distinguish adipocyte from other cells, gimsa and he could determine the stage of the adiopcyte differentiation through the number of lipid drop, size and the position of the nucleolus of the staining fat cell

    經過多次實驗，確定本實驗室大鼠前體脂肪細胞的最佳培養條件是：溫度為37 ，濕度為95 ， co _ 2濃度為5 ， ph值為7 . 0 7 . 2 ，離心力為1000r / min ，培養基為m _ （ 199 ）培養基，胎牛血清濃度為10 ，合適細胞接種密度為4 10 ~ 4 、 5 10 ~ 4個/ cm ~ 2 ，染色結果表明：油紅o染色是鑒定脂肪細胞的特異方法， gimsa和he染色可根據不同區域染色程度、著色差別判斷細胞核的位置及脂滴大小、多少，觀察大鼠前體脂肪細胞分化過程中的形態變化，進而確定脂肪細胞的分化階段。
  5. Since the success of dolly, the first cloned sheep with the adult somatic cells as karyoplast donor, new approaches have been developed for nuclear transfer technology. here we describe a handmade cloning method which combines the chemical induced enuleation and zona - free technology in embryo culture. enuleated oocytes were derived by exposing the oocytes to demecolcine and cytoheximide supplemented mdium sequently and its chromosome was depleted to the first polar body

    將培養10h的化學去核卵母細胞與供體成纖維細胞融合后lh 、 2h 、 3h ，分別有77 . 6 % 、 70 . 6 % 、 58 . 9 %重構胚的染色質發生凝集，其餘胚胎的染色體則處于原核期;而只在融合后3h ， 27 . 9 %重構胚被標記出組裝的紡錘體，且其中的同源染色體己經分離。
  6. The changes of above show that the damage to the chicken embryo caused by the chicken parvoviruses were widly. the pathogeny of infectious stunting syndrom was prolifirated in cells nucleus, it was a dna virus

    以上變化說明了分離病毒對機體損傷的廣泛性。核內包涵體的出現進一步說明，雞傳染性生長障礙綜合征的病原在細胞核內增殖，為dna病毒。
  7. At the beginning, the cytoplasm is evently distributed, then it is polar - distribution and at last it only remains between the apical cap and the sperm nucleus. during the period of spermatogonium, chromatin in the nucleus is in the shape of conglomeration and the nucleus is big ( i. e. the proportion of the nucleus to cytoplasm is bigger than spermatocyte ). at the stage of primary spermatocyte, chromatin accumulates to sc complexity. the nucleus of the mature sperm takes the shape of a plate and filmentous chromatins exist freely in uncondensed nucleus

    精子發生過程中，細胞器由少到多，到最後解體；細胞質從均勻分佈，到精細胞期極性分佈，最後殘留于成熟精子的頂帽和精核之間；細胞核在精原細胞期，染色質異固縮，胞核大，即核質比大於后續的精母細胞；在初級精母細胞中，染色質凝聚為sc復合體，到成熟精子，精核呈碟狀，染色質呈絲狀游離于非濃縮的核中。
  8. Mutated plasmid was transformed into e. coli tg1 cells to produce engineered peptide, then the peptide was purified by cm sepharose ion - exchange column. in vitro bactericidal assay and drug withdrawal were used to identify the bioactivity of the engineered peptide. the planar lipid bilayer membrane was used to assay the electrophysiology of the engineered peptide. toxicity studies on mammalian cells were used to assay the toxicity of the engineered peptide

    將重組質粒轉化入大腸桿菌tgi工程菌中，生產構建的工程多膚，離子交換純化后獲得工程多膚初步純化產物，體外抗菌試驗、藥物撤離試驗檢測工程多膚的抗菌活性，在人工脂質膜上測定其形成離子通道的特性以初步研究抗菌機理， ?並觀察其對真核細胞的毒性作用。
  9. Results showed : ( 1 ) cbt cell death in low tempratures is accompanied by characteristic changes, such as, reduced cell size, distorted nucleus, chromatin condensation and margination and cell ( cytoplasmic ) vacuolization ; cell mortality and ca2 * concentration increase along with time passed in low temperature. mitochondrial membrane potential and 02 increased at first, and then decreased. activities of sod decreased at first, followed by significant increasing and finally depressed

    結果表明： （ 1 ） cbt在低溫協迫下，細胞圓縮，細胞核變形，染色質濃縮且邊位，細胞質空泡狀；細胞死亡率隨處理時間的增加而增加；細胞內鈣離子濃度隨處理時間延長而遞增；線粒體膜電位差在低溫處理早期急速上升，隨后一直下降；細胞內超氧陰離子（ o _ 2 ~ - ）在低溫處理前期出現高峰，接著呈下降趨勢；細胞內sod活性在低溫處理前期減弱，接著上升，然後持續下降。
  10. 3 ) the results of studying on the chromosomal mutation and mitosis of the wheat irradiated by uv - b indicated that the rate of mitosis was inhabited by uv - b radiation ( 10. 08 kj ? m " 2 ? d " 1 ) in wheat cells, and some types of chromosome aberration were induced

    （ 3 ）對染色體及細胞分裂的研究結果表明，增強uv - b輻射能抑制小麥細胞的有絲分裂率，產生落後染色體、染色體橋、游離染色體、核變形等畸變。
  11. Section four : effects of copper and cadmium on ultrastructure of myocardial cell in sinopotamon yangtsekiense the effect of copper and cadmium on ultrastructure of myocardial cells of sinopotamon yangtsekiense was studied by us

    銅、鎘聯合作用30d后，細胞核形態進一步改變，內外膜分離程度加大，異染色質疑集加重。線粒體膜破裂，內容物外流，嵴斷裂消失。
  12. The ribosomes, made up of rna and protein, are found either lining the endoplasmic reticulum or free within the cell

    核糖體由蛋白質和rna組成，有的排列在內質網上，有的則在細胞中呈游離狀態。
  13. Recently, human elongator complexes have been isolated. the human complex is a fragile six - subunit complex that interacts with rnap ii and has hat activity directed against histone h3 and h4. depletion of elongator from hela nuclear extracts reduces the ability of these extracts to transcribe chromatin templates, providing biochemical support for the proposed role of elongator during transcript elongation in chromatin

    近來人elongator也被分離，它也是個不穩定的六亞基復合物，可與rnap相互作用，具有hat活性，能乙酰化h3和h4 ，在hela細胞核抽提物中去除elongator可降低抽提物對染色質模板的轉錄延伸速率，提供了該復合物在染色質為模板的轉錄延伸中有功能的生化證據，但是上面的信息都來自體外研究。
  14. In the second trial, this modified discontinuous percoll gradient centrifugation method was introduced to isolate spermatids from the semen of fifteen male infertile patients. then the effect was identified by wright - giemsa stain, flow cytometry analysis, immunocytochemistry and fluorescence in situ hybridization ( fish ). similary, the 22 % percoll fraction contained mostly haploid cells [ ( 91. 85 ? 5. 18 ) % ] ( p < 0. 005 ) and the mean density in this fraction was ( 1. 010 ? 0. 786 ) x 105 / ml

    C法，對15例各種類型不育患者的精液細胞進行分離，並利用瑞姬染色法、流式細胞術、免疫細胞化學和熒光原位雜交oisffi等方法，從細胞形態特徵、 dna倍體、細胞表面標i己與分化抗原，以及原位雜交信號的數目和位置結合細胞核特有的形態等方面加以鑒定。
  15. To make clear the hypothesis, a middle cerebral artery occlusion ( mcao ) and hypoxia and glucose - deprivation ( hgd ) ischemic models were used in in vivo and in vitro study, respectively. we first studied the cellular localization of kvl. 2 and the co - localization of kvl. 2 protein and vegf receptors flk - 1 and flt - 1, observed the effect of mcao on kvl. 2 expression and phosphrylation in the rat brain in vivo, then investigated the effect of vegf on ischemia / hypoxia cell damage and tyrosine phosphorylation of kvl. 2 in sh - sy5y cells. finally, in order to further elucidate the relationship between vegf ' s neuroprotection and its regulation on kvl. 2 phosphorylation, we used a specific antisense oligodeoxynucleotide ( odn ) to knockdown the expression of endogenous vegf to observe its role in ischemia / hypoxia cell damage and regulation of kvl. 2 phosphorylation

    為了驗證上述假設，本文分別在整體和離體水平，採用大腦中動脈缺血（ middlecerebralarteryocclusion ， mcao ）和體外氧?糖剝奪（ hypoxiaandglucose - deprivation ， hgd ）缺血模型，首先了解了kv1 . 2蛋白的細胞定位及與vegf受體flk - 1和flt - 1的共存情況，觀察了整體mcao后缺血再灌不同時間大鼠腦內kv1 . 2蛋白的磷酸化水平變化，然後通過外源性給予vegf蛋白，在sh - sy5y細胞株上觀察其對缺血細胞存活率及kv1 . 2蛋白磷酸化水平的影響，最後利用vegf反義脫氧寡核苷酸（ oligodeoxynucleotide ， odn ）特異阻斷內源性vegf蛋白的表達，觀察內源性vegf蛋白在缺血細胞損傷及調節kv1 . 2蛋白磷酸化中的作用，以進一步明確vegf缺血保護效應與其調節kv1 . 2蛋白磷酸化之間的關系。
  16. Dissociation and culture research of porcine donor cell and cell subculture in somatic cell nuclear transfer

    豬核移植供體細胞的分離培養及傳代的研究
  17. The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action, thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained. in this study, we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template. the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons

    本研究設計以c tamrna為模板的反義cdna片段，從c ta基因5 』端第94位到500位核苷酸段設計引物，目的片段407bp ，覆蓋第116和188位兩個aug密碼子，也包含了第一外顯子和第二外顯子間的剪接位點：用常規分子生物學方法構建了反義片段的腺病毒表達載體（ padeasy - 1系統） ；腺病毒載體經hek293細胞包裝產生含反義片段的重組腺病毒，用氯化銫密度梯度離心法獲得純化的高滴度腺病毒；進行體外基因轉移，分別用反義片段真核表達載體轉染p388d1細胞和用重組腺病毒感染hela細胞，觀察導入的c ta基因反義rna抑制細胞內組成型或誘導型c ta基因表達的作用，從而達到調控mhc -類分子表達的目的。
  18. In order to establish the technique of isolating es cell, and test the developmental ability of reconstructed embryos derived from es cell and oocytes of intra - or inter - species, mouse es cell were isolated from mouse blastocysts, and were used as donor nuclear, and were transferred into oocytes of various strains. the factors that influenced the development of reconstructed oocytes were determined. the developmental ability of embryos reconstructed with mouse es cells and rabbit oocytes was observed and analyzed

    為了建立胚胎幹細胞分離技術及了解其在同種和異種卵母細胞中的發育能力，首先從小鼠囊胚分離胚胎幹細胞，並以它作為核供體，移植到同種不同品系去核的小鼠卵母細胞內，觀察不同品系的卵母細胞對胚胎幹細胞重構胚發育的影響。
分享友人
分享到 Line

分享到臉書