電泳分離 的英文怎麼說
中文拼音 [diànyǒngfēnlí]
電泳分離
英文
electrophoretic separation-
The albumen - detected technology is based on the uv - absorption principle
依據紫外吸光度分析法，對蛋白質樣品進行電泳分離與紫外檢測。After quantification, the protein samples were separated by isoelectric focusing electrophoresis and then by sds - page
定量后，分別進行一維等電聚焦分離和二維聚丙烯酰胺凝膠電泳分離。Recovery of this photoinhibition is a complicate but orderly course, including degradation of photodamaged d1, synthesis and assembly of new one, etc. using lincomycin to block the replacement of new synthetic dl protein into photodamaged one, the spinach leaves was exposed to highlight, giving rise to photoinhibition before the thylakiod membranes were isolated
解除光抑制后， ps活性恢復是一個復雜而有序的過程，需要d1蛋白降解、新合成d1蛋白和重組裝ps等。實驗首先進行菠菜葉片光抑制處理，加入林可黴素阻斷葉綠體蛋白質合成，利用尿素sds變性電泳分離類囊體膜蛋白，藉助d1蛋白抗體westen免疫印跡、磷酸化蛋白快速檢測方法分析d1蛋白存在形式，並進行定量分析。Numerical study of the protuberance effects on capillary electrophoresis separation in the micro - channel
微通道壁面突起對片上電泳分離流動特性影響數值計算研究Molecular characteristic of salmonella serotype paratyphi a isolates in hangzhou by pulse field gel electrophoresis
杭州甲型副傷寒分離菌株脈沖場凝膠電泳分型Abstract ： the applications of chemometric approaches, such as opt imization, multivariate calibration, artificial neural network, signal processin g, resolution, parameter estimation etc., in capillary electrophoresis have bee n reviewed
摘要：綜述了最優化方法、多元校正方法、人工神經網路、曲線分辨、信號處理等化學計量學方法在毛細管電泳分離分析中的應用，展望了化學計量學在毛細管電泳中的應用前景。Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry
三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質We did the same steps for three times, so we could get the extraction using the steps mentioned. laemmli sample buffer was added to the extracted protein, which were then boiled for 5 min. the protein samples were separated by 12 % sds - page and transferred to nitrocellulose membrane
樣品蛋白經12 % sds一聚丙烯酞胺凝膠電泳分離后，轉印到硝酸纖維素膜上，與第一抗體4孵育過夜，經tbs洗滌后，再與第二抗體室溫孵育2小時， ttbs充分沖洗后，顯色觀察。A mixture of three amino acids ( arg, gly, glu ) labeled with fluorescein isothiocyanate ( fitc ) was separated in pdms microfluidic chip, the separation voltage is 200v / cm, the separation time is less than 120 seconds ; according to ccd fluorescence images, two distinct physical processes - stacking and destacking during sample injection were studied qualitatively ; rhodamine b, a kind of temperature - dependent fluorescence dye, was used as probe to develop a temperature - fluorescence intensity equation, then temperature - color map in microchannels was constructed, and temperature trait in microchannels on the pdms microfluidic chip was analysed. according to the results, we conclude that the electric field applied to the pdms microfluidic chip should not exceed 400v / cm
利用pdms微流控晶元對fitc標記的精氨酸、甘氨酸、谷氨酸混合物進行了電泳分離，分離電壓為200v cm ，分離時間不到120秒；通過拍到的熒光顯微圖像對電泳注樣過程中復雜的樣品分子積聚與解聚現象作定性的分析；以熒光染料rhodamineb為溫度熒光探針，建立了pdms微流控晶元上的溫度-熒光強度的關系公式，並利用matlab圖像處理工具箱構建出微流體溝道內的溫度色圖，對pdms微流控晶元的微流道溫度特性進行了分析，根據實驗結果，我們認為對于pdms微流控晶元來說，在進行需要外加電場作用的試驗時，外加電場不應超過400v cm 。Isolation and identification of venom of bungarus multicinctus from hunan by ce
銀環蛇蛇毒毛細管電泳分離與鑒定The pcr products were electrophoresed on 3 % agarose gel and visualized under uv light
聚合酶鏈鎖反應的產物以3 %瓊脂電泳分離並在紫外光下觀察結果。The relative theory of capillary electrophoresis has been studied. the low voltage separation models with moving grade electrical field and high voltage separation model have been discussed
研究了毛細管電泳的相關基礎理論，從理論上較深入地研究分析了傳統的毛細管高電壓分離模式與本文所採用的低電壓運動梯度場分離模式。After being concentrated and desalted, the samples were separated by isoelectric focusing on first dimension and sds electrophoresis on second dimension
樣品濃縮除鹽后，進行一維等電聚焦分離和二維sds電泳分離提取人腎組織磷酸化蛋白。I and hindlll respectively. after the digested products were run via agarose gel electrophoresis and transferred into nylon membrane, the southern blot was carried out using the cdna of rubber tree etrl as probe. the result of the southern blot showed that a hybridization band ( - 3. 0kb ) turned up from the ecor i digested product and another band ( - 4. 8kb ) turned up from the hindi ]
從橡膠樹pr107品系的嫩葉提取基因組dna ，用限制性內切酶ecor 、 hinofll分別酶切，瓊脂糖凝膠電泳分離並轉膜后，用克隆的橡膠樹etri基因的cdna片段作探針進行southern雜交分析，結果表明， ecor酶切在約3 okb處有一條雜交帶出現， hi 。The pcr amplification products were separated on 6 % vertical denaturing polyacrylamide gels. numerous and distinct bands could be detected by silver staining
Pcr擴增產物經6變性聚丙烯酰胺凝膠垂直電泳分離后，銀染能檢測到多而清晰的條帶。A method of separating substances, especially proteins, and analyzing molecular structure based on the rate of movement of each component in a colloidal suspension while under the influence of an electric field
電泳分離法分離物質，尤指分離蛋白質的方法，以及基於在電場作用下處于膠粒懸浮狀態的各個成分的運動速度基礎上進行的分子結構分析方法Abstract : technical progress of uranium extraction in recent years is introduced, including new processes such as uranium extraction by supercritical co2 fluid, calixarene, capillary electrophoresis, oscillation ; application of novel extractants and absorbents ; and three wastes disposal
文摘:介紹了近年來鈾提取方面的技術進展，包括提鈾新工藝:超臨界co2流體提取鈾，杯芳烴提鈾，毛細管電泳分離鈾，振蕩法萃取鈾；各國新型萃取劑和吸附劑的應用及三廢治理。Indentificatiort is also the first step towards studies on protein co - and post - translational modification, and ultimately, function. in the present study, the total proteins of the photo - thermo sensitive genie male - sterile rice ( oryza sativa, peiai64s ) spikelet at meiosis stage were used as the material. by optimizing crucial factors and procedures such as sample treatment, electrophoresis parameters, and gel concentration, 2 - d maps with high quality and reproducibility were obtained
用兩種方法對經雙向電泳分離的凝膠上的蛋白質點進行了初步鑒定，一是通過電印跡轉移把蛋白質轉到pvdf膜上，再用edman降解的方法測得部分相對分子質量在10000 - 30000da的蛋白質點的n -端序列，通過網上搜索其同源性對其進行鑒定，並確定該點在凝膠上的位置。With advantages of high efficiency, real time, low volume and automation, capillary electrophoresis separation techniques have been widely used in industrial analysis, biomedicine, molecule biology etc. with the pretence of lab on the chip and the development of micro total analysis system, electrophoresis chip, capillary electrophoresis technology on a chip, became the research hot of micro electromechanical system
毛細管電泳分離技術以其高效、快速、微量、易自動化等優點在生命科學、醫學藥物、環境保護等領域得到了極其廣泛的應用。隨著「晶元上的實驗室」思想的提出和微型全分析系統的發展，使得電泳晶元?在晶元上進行電泳分離的技術成為了當今國內外的重要研究熱點。Micro - channel electrophoresis chip ( mce chip ) system is a newly developed research field. the micro - channel network on the chip is manufactured by the technology of micro opto electro mechanical system ( moems ) on the substrate of glass, fused silica, pdms etc. sample separation is performed in the micro - channel and detected by opticcal or chemical methods
微通道電泳晶元（ micro - channelelectrophoresischip ， mcechip ）系統是利用微光機電系統（ moems ）技術在玻璃、石英、有機聚合物等基片上刻蝕出預設計好的微通道網路，並在其中進行樣品的電泳分離，利用光學或化學等方法進行檢測。分享友人