電泳性能 的英文怎麼說

中文拼音 [diànyǒngxìngnéng]
電泳性能 英文
electrophoretic property
  • 電 : Ⅰ名詞1 （有電荷存在和電荷變化的現象） electricity 2 （電報） telegram; cable Ⅱ動詞1 （觸電） give...
  • 泳 : 動詞（游泳） swim
  • 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
  • 能 : 能名詞（姓氏） a surname
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. It manufactures various high performance industrial coatings, including coil coatings, wood coatings, general industrial coatings liquid coatings, powder coatings, electrocoat, auto refinish coatings, mirror coatings, etc

    公司生產各種高性能的工業塗料，包括卷鋼塗料、木器塗料、一般工業漆塗料（農用工業機械液體塗料、粉末塗料、電泳塗料） 、汽車修補漆、鏡面塗料等。
  3. Using diethanolamine as aminating agent and glacial acetic acid as neutralizing agent, aminated epoxy acrylic cationic resin was prepared. the effect of technology of aminated epoxy acrylic resin on properties of eletrodeposition was studied by conductivity meter and electrophoresis apparatus. it was shown that, conductivity firstly decreased, and then increased with aminating temperature increase. in contrast with putting polyacrylic resin into thin acetic acid solution, the more compact film could be achieved by neutralizing polyacylic resin with glacial acetic acid and then add it into water. when neutralizing temperature was enhanced, the speed of electrodepsidon was found to increase, and the film was also more compact. increasing the dn leads to enhanced conductivity and smaller particle size. when dn equaled to 80, the smoothest film could be achieved

    以二乙醇胺為胺化劑、冰醋酸為中和劑，合成了胺化環氧丙烯酸陽離子樹脂.採用電泳儀和電導率儀，研究了胺化環氧丙烯酸樹脂合成工藝對陰極電泳塗料電沉積性的影響.結果表明，隨著胺化溫度的增加，電泳液電導率先下降後上升.將冰醋酸加入樹脂中中和，後用水稀釋，比樹脂在醋酸稀溶液中中和，電沉積性能更好.電沉積速率隨著中和溫度的上升而增加，電沉積膜緻密性相應增加.中和度（ dn ）愈高，電泳液電導率愈大，粒徑越小，而塗膜外觀在中和度為80時達到最佳
  4. After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s

    通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。
  5. . moreover, some samples appeared several active bands in the gel, which indicated the existence of different types of sod or multi - subunits of sod in these samples. the bacterial strain 276 is a gram - negative rod bacterium and there are more than 3 polar flagella, which observed after the gram ' s staining and flagellum staining

    同時，利用非變性聚丙烯酰胺凝膠（ page ）電泳后的凝膠顯色反應，發現一些樣品出現了多條活性帶，這可能是因為在這些細菌提取物樣品中含有不同類型的sod分子，或是同一類型的sod含有多個亞基組成。
  6. We also investigated the pathological changes of mouse liver, thymus and cerebrum cortex challenged by so2 inhalation by in vivo tests. we studied the apoptotic induction on mouse spleen cells and cytotoxicity of human embryo lung fibroblasts of so2 derivatives by in vitro tests. in vivo tests of sulfur dioxide inhalation showed : ( 1 ) effects on mouse lung of so2 challenge : we found no significant apoptotic changes induced by so2 inhalation but obvious pathological changes of lung with vacuolating of osmiophilic multilamellar bodies which maybe related with the decrease of surfacant and decrease of microvillus of type ii alveolar cells ; we also found thickening of part of basement lamina between type i alveolar cells and capillary endothelium cells which may inhibit the dispersion of oxygen and contribute to lung dysfunction

    二氧化硫熏氣染毒的體內實驗結果表明，在本次實驗的濃度范圍內（ 56mg m ~ 3 、 112mg m ~ 3 、 168mg m ~ 3低、中、高三個濃度） ： （ 1 ）通過透射電鏡、 dna凝膠電泳分析和流式細胞分析發現二氧化硫吸入染毒一周對小鼠肺臟沒有明顯的凋亡誘導作用，但通過透射電鏡觀察發現二氧化硫可引起肺臟明顯的超微結構改變，引起型肺泡上皮細胞板層體空泡化，微絨毛減少，線粒體緻密化或腫脹變性；肺泡血管內皮細胞和型肺泡上皮細胞之間基膜增厚，使氧氣彌散功能出現障礙，從而降低肺功能。
  7. The results of lauryl sodium sulfate - polyacrylamide gel electrophoreses ( sds - page ) of the aggregate precipitate and supernatant and the result of high - performance size - exclusion chromatography of the supernatant indicated that, by wrongly linked intermolecular disulfide bonds soluble bi - molecular and tri - molecular egg white lysozyme aggregate could be simultaneously formed except being renatured to native and active egg white lysozymes during the refolding procedure of denatured - reduced egg white lysozyme ; the aggregate precipitate could be further formed by the non - covalent bonds interaction between the soluble hi - molecular egg white lysozyme aggregates, and the soluble tri - molecular egg white lysozyme aggregate could still stay at the supernatant

    沉澱和上清液的不連續十二烷基硫酸鈉聚丙烯酰胺凝膠電泳（ sds - page ）和高效凝膠排阻層析分析結果表明，還原脲變性蛋白溶菌酶在稀釋復性過程中除了能夠復性成天然態蛋白溶菌酶分子外，還會形成可溶的蛋白溶菌酶分子二聚體和三聚體，二聚體和三聚體主要是靠分子間二硫鍵的錯配連接而成的；可溶的蛋白溶菌酶分子二聚體之間通過非共價鍵相互作用而形成集聚體沉澱，而可溶的三聚體溶菌酶分子則仍處于復性液上清液中。
  8. The general analysis of dip - dropping, spin - coating method and electrophoretic deposition techniques shows that the compaction degree of alumina film fabricated by the former two methods are higher than those prepared by the last one. besides, spin - coating method is the most efficient and fast way to raise film thickness

    綜合分析提拉法、旋覆法和電泳沉積工藝，採用前兩種工藝制備的氧化鋁膜的緻密性優于末者，並且三者中旋覆法最能快捷有效地提高膜厚。
  9. This understand of stored nitrogen compounds restricted seriously the progress in the investigation of vegetative storage proteins. in the dissertation, we studied more extensively the cytology, biochemical properties and biological roles of vegetative storage proteins in swietenia macrophylla and in hevea brasiliensis by light - and electron microscopy, sds - page, page, immuno - blotting, indirect immunohistochemical localization and colloidal gold labelling and cdna clone techniques

    採用光鏡和電鏡技術、 page 、 sds - page和免疫印跡技術、電泳凝膠過碘酸? schiff試劑染色、間接免疫熒光和電鏡免疫細胞化學定位技術以及cdna克隆技術，較深入地研究了大葉桃花心木和巴西橡膠樹的營養貯藏蛋白質的細胞學、生物化學性質和生物學功能。
  10. All of our products are manufactured with advanced chemical techniques high class directions for producing and part of raw materials imported from other countries and through strict quality management. at the same time, with a view to protecting environment and customer s health, all of raw materials we apply are environment - protecting, low or no noxious. we band ourselves to most advanced producing techniques for our customers

    本公司生產的br - bt表調劑具有表調效果好，處理面積大1500平方米公斤，穩定性高，抗硬水能力強等優點，與各種配方的鋅系磷化液均有優良的配伍性，適合於噴漆噴塑電泳的前處理，已大量應用於汽車冰箱空調自行車等行業，獲得廣泛好評。
  11. One 66kd band appeared except 44kd main band when go isozyme above was subjected to sds - page and ce - sds, indicating this go isozyme was similar to that from spinach leaves which contained 40kd and 66kd simultaneously. whether b - mercaptoethanol was added or not when go isozyme was subjected to in sds - page and ce - sds, 40kd main band and 66kd band still appeared, indicating two subunits were not linked by covalent disulfide. amino acid analysis shew that the ratios of basic to acidic amino acid of go isozyme and its 40kd acidic subunit were 0

    菜心go同工酶的sds - page和sds -毛細管電泳（ ce - sds ）顯示，該酶除了含40kd主帶外，還有很淺的66kd帶，和之前我們提出的菠菜go同工酶含40kd酸性亞基和66kd堿性亞基相似； sos - page和ce - sds電泳中，無論加入-巰基乙醇與否， go同工酶都只有40kd主帶和66kd淺帶，表明菜心go同工酶中40kd酸性亞基和66kd堿性亞基不是以共價二硫鍵相連；用制備性sds - page法獲得菜心go同工酶的40kd亞基，並和菜心go同工酶一起測定其氨基酸組成，該go同工酶及40kd亞基的堿酸性氨基酸的比例分別為0 . 66和0 . 54 ，表明40kd亞基可能是個酸性蛋白，而66kd帶則是個堿性蛋白。
  12. Isoelectric focusing a technique used in electrophoresis to separate amphoteric molecules ( able to combine with either acids or bases )

    等電點聚焦：是一種利用電泳技術分離兩性分子（能與酸或堿結合）的技術。
  13. Organic surface modification of titanium dioxide and electrophoretic properties of the resulting materials

    2的有機改性及其電泳性能
  14. This paper presents comparative systematic research of problems in the preparation of economical mould pressing bonded ndfeb permanent magnets, which include the fabricating of economical rapidly - quenching ndfeb magnetic powder and mould pressing bonded ndfeb permanent magnets ; the effect of the ndfeb magnetic powder on the magnetic properties of the magnets and the effect of the cathode electrophoretic coating on the corrosion resistance of the ndfeb magnets

    為了充分發揮我國稀土資源優勢，發展我國的ndfeb稀土永磁產業，本文比較系統地研究了高性價比的模壓成型粘結ndfeb永磁體制備過程中的相關問題，包括高性價比快淬ndfeb磁粉制備、模壓成型粘結ndfeb磁體制備、 ndfeb磁粉特性對粘結磁體磁性能的影響、 ndfeb磁體陰極電泳塗層工藝及其塗層對磁體抗蝕性的影響。
  15. The pcr amplification products were separated on 6 % vertical denaturing polyacrylamide gels. numerous and distinct bands could be detected by silver staining

    Pcr擴增產物經6變性聚丙烯酰胺凝膠垂直電泳分離后，銀染能檢測到多而清晰的條帶。
  16. The homologious comparison proved the cloned gene had 96 % homology to the sequence of the omp gene, and the alignment of the amino acid sequence was 98 %. the recombinant plasmid was constructed with the target gene and the expressing vector pgex - 4t - l and then was transformed into e. coli bl21 ( de3 ) the fusion protein was expressed under the iptg inducing condition, and exhibited about 62kda in size, very close to the predicted molecular weight of gst - momp. furthermore, the fusion protein was specifically recognized by anti - serum which raised against the major outer membrane protein of ahl316

    Sds - page電泳分析顯示誘導表達的基因產物分子量約為62kda ，與預測的gst -外膜蛋白重組融合蛋白的分子量極為相似， western - blot進一步證實，表達產物能被嗜水氣單胞菌l316主要外膜蛋白特異性抗血清所識別，產生明顯的染色條帶，說明所表達的基因產物與天然的外膜蛋白抗原性一致。
  17. The enzyme was purified from cell extract by ammonium sulfate fractionation ( 30 % - 80 % saturation ) and consecutive column chromatography using deae - cellulose and sephadex g - 100. the sod activity for purified enzyme could reach 4966 iu / mg proteins, and the molecular weight of the sod was about 20 kda which determined by sds - page electrophoresis. when the non - denaturing polyacrylamide gel stained with substrate for sod in the present of 0. 2mmol / l kcn or 0. 5 mmol / l h2o2, the active band was still showed at the same position, which indicated the sod could not be inhibited by the treatments with kcn or h2o2 and it was the mn sod

    通過sds一聚丙烯酸胺凝膠電泳可知該sod酶的分子量約為20kda .在非變性聚丙烯酞胺凝膠（ pagb ）電泳后，在凝膠son顯色反應液中加入0 . 2耐01 / lkcn或0 . 5inmol / lh202 ，凝膠sod顯色反應后在原來的sod酶部位仍然含有sod活性帶，這表明利用kcn和h20 :處理並不能抑制500的活性，該sod屬于mn一sod 。
  18. With most advanced manufacturing equipment and facilities, tremendous strength and sophisticated testing equipment, we can manufacture magnet with a variety of shapes, sizes and dimensions at the requests of customers, have nickel, zinc, tin, epoxy and aurum coatings applied to magnet. some of them are highly anti - corrosive

    公司設備先進，實力雄厚，擁有現代化的生產檢驗設備。能生產各種大小、尺寸、形狀的釹鐵硼磁體產滿足不同用戶的需求。磁表面電鍍有：鍍鋅、鎳、錫、電泳環氧、金等，產品抗氧化性極強。
  19. This gives huanyu most advantages in paint industry in china. huanyu is engaged in the environment protection at all times, for example, in the area of reuse of waste polymerisates, in the area of making aqueous paint to restrict voc let out. especially in the reuse of polymerisates, by degradation, the macromolecule become oligomer which can be used as materials in paint resin manufacturing, both pet flack, grain, silk, bottle, block appearance and ps espccially in bubble form be widely used in huanyu. besides, huanyu has been using waste pe, pvc, pp to make aqueous paint package, the output reached 1 million size 5 gallons in 2000

    公司主要產品包括：醇酸硝基聚酯聚氨酯氨基環氧陰極電泳氯化橡膠丙烯酸苯乙烯瀝青內外墻乳膠漆等12大系列， 1000多個花色品種，年生產塗料油漆2 . 6萬噸，產品的各項性能指標均達到或超過國家標準，並先後榮獲「中國綠色建材產品」 「中國環保產品質量信得過重點品牌」 「山東省名牌產品」 「山東省技術監督局免檢產品」 「濰坊市技術監督局免檢產品」等榮譽稱號。
  20. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
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