電泳純的 的英文怎麼說
中文拼音 [diànyǒngchúnde]
電泳純的
英文
electrophoretically pure-
Our previous studies showed existence of apoplast cam in the plant cell and cam had many extracellular functions. so it supposed cam may be one of important extracellular polypeptides and trigger the intracellular signal transduction by binding the receptor. in this study, radiolabelled ligand is used to investigate the binding characteristic of cam and a. thaliana protoplasts. and chemical crosslinking is employed to explore binding proteins in the membrane. at first, ( 35 ) ~ s - cam was produced using ( 35 ) ~ s - labeled amino acid mixture in e. coli. sds - page and autoradiograph indicated high - purified, high - specific radioactivity ( 35 ) ~ s - cam was obtained. electrophoresis of ( 35 ) ~ s - cam is the same as that of unlabeled cam with ca ~ ( 2 + ) or egta ; a quatitive of protoplasts was prepared by enzymolysis
首先，用~ （ 35 ） s標記的氨基酸混合物喂養工程菌成功地制備了~ （ 35 ） s標記的擬南芥鈣調素（ ~ （ 35 ） s - cam ） ， ~ （ 35 ） s - cam純度高、放射活度高、 ca ~ （ 2 + ）與egta存在時的電泳行為與未標記cam相同，可作為一種高靈敏性的探針用於進行受體學分析實驗；用擬南芥種子誘導愈傷，通過酶解制備了大量原生質體。To prove up ulteriorly the components of ants, the author has separated and purified the polypeptides of formica rufa linnaeus and studied their biologic and officinal activities. at the same time, the author has mensurated the molecular weight of polypeptides by sds polyacrylamide electrophoresis to analyze the polypeptides quantific - ationally. in order to provide the scientific basis for studying and empoldering the ants, we have done these studies
為進一步探明螞蟻體內的活性成分以開發和利用其藥用價值，本文對紅褐林蟻的提取物中的多肽進行了分離、純化並對其生物活性和藥用活性進行了研究，通過電泳測定了多肽的分子量，從而為進一步研究開發螞蟻提供科學的依據。6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page
將枯草芽孢桿菌超聲波破壁后的粗提取物進行分段鹽析、 deae - sepharose陰離子交換柱層析， blue - sepharosecl - 6b特異結合柱層析和sephadexg - 200凝膠過濾等純化步驟，得到聚丙烯酰胺凝膠電泳為單一蛋白區帶，比活為1 . 46u mg的酶制劑。The substance with antibacteria action obtained from forest frog is made up of alanine, aminoacetic acid, leucine, isoleucine, proline, aminoglutaric acid, threonine, serine, lysine. the substance with antibacteria action is a kind of poly peptide with a micromolecul
純化的林蛙皮膚抗菌活性物質經尿素? sds ? page電泳分析，表現為一條帶，分子量約為6 . 28kda 。In the part, the author ' s intention is mensurating the molecular weight of the polypeptides by sodium dodecyl sulfate polyacrylamide gel electro - phoresis. the result shows that one of the polypeptides which we have mensurated is about 17, 000da. the conclusion is that the components which we have separated and purified are small molecule polypeptides. 5
紅褐林蟻多肽蛋白質的分子量測定本部分旨在通過sds -聚丙烯酰胺凝膠電泳測出所得多肽蛋白質的相對分子量，測得其中一組分的相對分子量為17000da左右，結論為所分離、純化的物質是小分子的多肽。The purity was detected by sds - page, and only igg ' s heavy chain and light chain protein tope were showed up. the activity of igg was tested by technique of immune credeschs gold
用sds - page進行igg的純度檢驗，電泳結果出現兩條蛋白帶，是igg的重鏈和輕鏈，說明純化的程度較高。However according to the view of acoustics, click includes mixed frequencies, so further studies are necessary to investigate the physiological significance of amygdaloid modulating effect on the ascending auditory information at the cortical level. in this paper we firstly observe the characteristics of the acoustic response of neurons in a - i evoked by pure tone ; then investigate the influence of la stimulation on the acoustic response of these neurons and the physiological significance of such influence ; revea l the neural pathway mediating this effect with the neurohistological method ; and study the neurotransmitter and its receptor participating in this effect
本文採用電生理學方法考察大鼠皮層a區神經元純音反應特徵，觀察杏仁外側核（ la ）對a區神經元聲反應的影響，運用神經組織學方法揭示介導這一影響的神經環路，採用多管微電極記錄結合微電泳技術的方法研究參與這種影響的神經遞質及受體，進一步探討了這種調制性影響的生理學意義。Medium experiments were arranged under uniform design, and then an optimum medium was got accordingly. the culture liquid was centrifugalized at 3, 500r / min for 30min, then ammonium sulfate was added into the supernatant to a final concentration of 30 % to precipitate the others
通過硫酸銨分級沉澱、 deaesephadexa - 50陰離子交換凝膠層析和sephadexg - 75凝膠柱層析對發酵液進行分離和純化，並得到電泳純的酶。Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry
三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質The expressed product was lysised with supersonic wave and purified by sds - page. elisa analysis revealed that the antigenicity of the vpi protein has been detected. the forth part - detection of aev - nh937 strain by in situ hybridization ( 1sh ) - probe was labelled with digoxigenin ( dig ). then the probe hybrid with 5d, 10d, and 20d postinfection brain tissue of chicken. the results of the ish showed that the positive signal was found in 3 cases, while control group was negative. there has been a reasonable correlatien between this method and other detection test
經超聲波裂解，用尿素溶解包涵體，電泳純化后，利用elisa檢測vp _ 1外殼蛋白，表明具有一定抗原性；第四部分：應用原位雜交檢測aevi用dig標記的探針與sd 、 10d 、 20d的攻毒雞腦組織雜交，來檢測那v的rna 。By sds - page and immuno - blotting, the monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain could recognize the 67 kda protein in purified golgi apparatus fraction from lily pollen. subsequently by immuno - gold labeling and transmission electron microscopy, we found that the dynein intermediate chain - like protein bound mainly to the membranes of golgi - associated vesicles. statistics analysis of dynein intermediate chain - like protein on golgi - associated vesciles showed the nearly equal chance of distribution on either cis - or trans - golgi - associated vesciles
對分離純化的百合花粉及花粉管中高爾基體組分進行sds -聚丙烯酰胺凝膠電泳和免疫印跡發現，抗雞腦細胞質力蛋白中間鏈單克隆抗體在67kda處有較強的免疫交叉反應；進而通過免疫金標結合電子顯微鏡觀察發現，大多數類細胞質力蛋白中間鏈存在於高爾基體附近的囊泡膜上；統計結果表明，類細胞質力蛋白中間鏈在順面和反面高爾基體附近囊泡膜上的分佈機率大致相等。Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution
3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵，用緩沖液抽提后，經硫酸按沉澱， deae一纖維素離子交換層析，聚丙烯酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸酶，用sds一page檢測為一條均一譜帶，其分子量約為78kd 。Our efforts were taken to lay the foundation of further studies on cloning and function of new genes in fish. the construction and evalution of the a, gtlo cdna library of grass carp leukocytes are described. total rna was extracted from head - kidney leukocytes using single - step method of rna isolation by acid guanidinium thiocyanate - phenol - chloroform extraction
本文第一部分取病毒感染36小時后的草魚頭腎並分離白細胞，用改進的異硫氰酸胍一步法從其中提取總rna ，磁珠法分離純化mrna ，電泳檢測其質量。In recent measuring method, protein electrophoresis is rapid, accurate, steady and economic, not affected by environment condition, we improve reactive system of our national tentative com protein gelatinous electrophoresis, and that which is more adapted to imaging process
在玉米種子純度檢測的方法中，蛋白質凝膠電泳法具有快速、準確、穩定、經濟、不受環境條件影響等特點，因此，選取此方法作為種子純度檢測方法。對國家試行的玉米蛋白凝膠電泳法的反映體系進行了改進，使得到的圖像更清晰。- acetolactate decarboxylase are widely found among bacterial strains but not in other groups of organisms. the enzyme has been demonstrated to be effective for removal of acetolactate and widely used in beer product. in this paper, - acetolactate decarboxylase from bacillus subtilis was purifed to homogeneity from cell extract by ammonium sulfate - fractionation, heat treatment, deae - sepharose fast flow column chromatography
本文對來源於枯草芽孢桿菌（ bacillussubtilis ） 3226 - 5的-乙酰乳酸脫羧酶經硫酸銨分級沉澱、熱處理、 deae - sepharosefastflow離子交換柱層析等分離純化步驟，得到sds - paeg電泳純，通過n末端氨基酸序列分析驗證酶蛋白的純度。Gst - eorabl fusion protein and truncated gst - deeorabl fusion protein were purified by gst affinity chromatography and analyzed by cleaving with thrombin protease
全長型融合蛋白經凝血酶酶切和進一步親和純化得到達電泳純的游仆蟲rab蛋白（ eorab1 ） 。The isolation and purification of dnaase in the earthworm the earthworm dnaase was purified from the tissue extract of earthworm by denaturing the protein with low ph buffer, high temperature, ammonium sulfate precipitation, deae - cellulose ( de52 ) chromatography and ultra - filter membrane
雙胸蚓組織中dna酶的分離純化雙胸蚓組織粗提取液經過選擇性酸變性、選擇性熱變性、硫酸按分段鹽析、 deae一纖維素（ de52 ）柱層析、超濾膜分級分離后得到一個電泳純的dna酶。Under these conditions the fibrinolytic activity of the supernatant can reach 820 urokinase units per milliliter broth. ba - dfe was purified from the supernatant of b. amyloiquefaciens dc - 4 culture broth by ammonium sulfate precipitation, ion - exchange chromatography on cm - and deae - sepharose fast flow, hydrophobic interaction chromatography on phenyl sepharose 6 fast flow and gel filtration on sephadex g - 50. the purified enzyme displayed thermophilic, hydrophilic and strong fibrinolytic activity
通過硫酸銨分級沉澱、 cm - sepharosefastflow和deae - sepharosefastflow離子交換層析、 phenylsepharose6fastflow疏水層析和sephadexg - 50凝膠過濾等方法，從解澱粉芽孢桿菌dc - 4的發酵液中分離純化出電泳純的ba - dfe 。The brine shrimp he was prepared and purified by 67 % ammonium sulfate precipitation, deae - sepharose fast flow ion - exchange chromatography, and sephacryl column gel - filtration, and its biochemical and enzymological properties were identified in this study. it was found that the deduced molecular weight of he in sds - page is about 98. 5 kda, and its proteolytic activity was optimized at ph of 7. 5 - 8. 5 and at temperature of 40, respectively
我們利用67硫酸銨沉澱、 deae - sepharosefastflow陰離子交換柱層析和sephacryl凝膠過濾柱層析，並以酪蛋白為其蛋白酶水解活性的檢測底物、以卵膜為其卵殼裂解活性的特異性底物，從鹵蟲胚胎孵化液中分離純化出了鹵蟲的孵化酶分子，其在sds - page電泳中的分子量約為98 . 5kda 。The crude cellulases from liquid fermentation of b - 6 and ass. 3711 were isolated and purified by ( nh4 ) so4 precipitation, sephadex g - 100 and deae - sepharose cl 6b column chromatography. the cmcase components were purified and some of their physical and chemical properties were studied
本文將液體發酵的酶液經硫酸銨分級沉澱、柱層析后得電泳純cmcase組分，並對as3 . 3711和b - 6來源的cmcase酶解動力學和理化性質作了比較研究。分享友人