電泳膠 的英文怎麼說
中文拼音 [diànyǒngjiāo]
電泳膠
英文
running gel-
Extracts of the hybrids were then subjected to polyacrylamide gel electrophoresis and tested for tk activity.
用聚丙烯酰胺凝膠電泳檢測雜種提取種物的TK活力。Analytical polyacrylamide gel electrophoresis apparatus
分析聚丙酰胺凝膠電泳儀An optimization scheme for two - dimensional gel electrophoresis
一種優化的雙向凝膠電泳方法Alternatively the gels may be destained electrophoretically.
凝膠脫色的另一方法是電泳法。Ampholytes are electrophoresed through the gel prior to sample addition.
在加入樣品前，兩性電解質先通過凝膠電泳。6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page
將枯草芽孢桿菌超聲波破壁后的粗提取物進行分段鹽析、 deae - sepharose陰離子交換柱層析， blue - sepharosecl - 6b特異結合柱層析和sephadexg - 200凝膠過濾等純化步驟，得到聚丙烯酰胺凝膠電泳為單一蛋白區帶，比活為1 . 46u mg的酶制劑。Mtt value, morphological characteristics under light and electron microscopy, dna gel electrophresis results, and hoechst33342 dying results show that inosine can protect nscs against apoptosis induced by hioi significantly in a dose dependent manner
2的直接保護作用，通過mtt值的測定，光鏡及電鏡的形態觀察， hoechst33342染色后核形的觀察及dna凝膠電泳結果，發現肌昔可對hzoz誘導的c門After quantification, the protein samples were separated by isoelectric focusing electrophoresis and then by sds - page
定量后，分別進行一維等電聚焦分離和二維聚丙烯酰胺凝膠電泳分離。Dna damages caused by so2 and lead acetate were studied with the single cell microgel electrophoresis technique ( or comet assay ) in order to confirm the damaging degree of lead ( as an important component of atmosphere particle matter ) on dna from male mice exposed to so2. the migrating distances of dna of brain, lung, spleen and kidney cells of mice increased significantly, compared to the control group under conditions of single and combined poisoning of so2 ( 42mg / m3 ) and lead acetate ( 0. 2 % ), and lead could strengthen dna damage degree by so2 in nuclear dna of brain, kidney, spleen cells. damaging degree of so2 on nuclear dna of lung cell of mice was more severe than that of lead
為了明確大氣顆粒物中的重要組分? ?鉛在二氧化硫所致dna損傷中的作用程度，利用單細胞凝膠電泳技術（ singlecellgelelectrophoresis ， scge ，或稱彗星實驗， cometassay ）研究了鉛與二氧化硫的聯合污染，結果表明在42mg m ~ 3so _ 2和0 . 2醋酸摘要一abstract鉛單獨及聯合染毒條件下，小鼠腦、肺、腎、脾細胞dna遷移距離均比對照顯著增加;鉛加劇了50 :對腦、腎、脾細胞核dna的損傷程度; 50 :對肺細胞核dna的損傷程度要比鉛的損傷大，小鼠肺細胞核dna遷移距離在50 :和醋酸鉛聯合作用組與醋酸鉛單獨作用組間有極顯著性差異（ p < 0 . 01 ） ，而與502單獨作用組間沒有顯著性差異。二 、 effects of calcium concentration on the membrane - bound gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation. the membrane - bound gq a was extracted from the retina of the macrobrachium rosenbergi with protein extract and was electophoresised by sds - page. the percent of the membrane - bound gq a was analyzed by tanon gis gel image disposal system
二、鈣離子濃度對光暗適應羅氏沼蝦感光細胞膜結合gq蛋白亞基的影響用蛋白質提取液提取膜結合gq蛋白亞基並sds - page電泳，利用tanongis凝膠圖像處理系統分析其含量。In our experiment, after light and dark adaptation, the retina of the macrobrachium rosenbergi was respective incubated in high calcium solution, physiological solution and low calcium solution. we studied the effect of calcium concentration on the content and subcellular localization of gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation by sds - page technology and imunoelectron microscopy technology. our study results indicated : 一 、 effects of calcium concentration on the soluble gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation
而鈣離子對gq蛋白亞基活性有無影響還未見報道。我們以光適應和暗適應條件下的羅氏沼蝦復眼視網膜為材料，分別用高鈣溶液、生理溶液、低鈣溶液孵育后，通過sds ? page電泳技術及免疫膠體金電鏡技術，研究鈣離子濃度對光暗適應時羅氏沼蝦感光細胞gq蛋白亞基含量的影響及亞基亞細胞定位的影響。We present a preliminary study on the zymograms of esterase ( est ), lactate dehydrogenase ( ldh ), malate dehydrogenase ( mdh ) from four groups of microtus fortis by vertical discontinous polyacrylamide gel electrophoresis method. the number, mobility and dye intensity of the enzyme band were analyzed
採用垂直板不連續聚丙烯酰胺凝膠電泳法對四類東方田鼠的酯酶（ est ） 、蘋果酸脫氫酶（ mdh ）和乳酸脫氫酶（ ldh ）進行了初步研究。First, three isozymic systems ( lactate dehydrogenase ( ldh ), esteraes ( est ), malate dehydrogenase ( mdh ) ) extracted from threepopulations of mandarinfishes were detected by discontinuous vertical plate polyacrylamide gel electrophoresis ( page ). several loci were tested but none polymorphic locus was detected in qiupu river population. the results showed that : ldh can be used as biochemical markers to identify these three populations of mandarinfishes
首先，本試驗採用聚丙烯酰胺凝膠電泳（ page ）對我省三個水域鱖魚群體3種同工酶（ ldh 、 est 、 mdh ）的不同基因座位進行了檢測，結果表明： ldh同工酶可以作為區分秋浦河鱖魚、長江鱖魚兩個群體與萬佛湖鱖魚群體的生化遺傳標記；其次，運用rapd技術分析了三群體鱖魚的基因組dna的多態性。( 2 ) the mortality of cp cell in 10 were increasing directly related with the time in the low temperature. after 120d some cp cells died in the way of apoptosis. most nucleus of cells were condensed, and chromosome was marginated beside the nucleus inner membrane, cell sizes were reduced, dna ladder showed in dna gel electrophoresis
（ 2 ） cp細胞系在10低溫下細胞死亡率與時間成正比， 120d的細胞具典型的凋亡現象，即細胞核固縮、染色體邊集在核膜內側；細胞體積變小；瓊脂糖凝膠電泳上顯示特徵性的「梯狀」帶。Ip3 - ip3 receptor ( ip3r ) interaction mediates the release of ca2 + from the endoplasmic reticulum in response to many different extracellular stimulus. for higher plants, however, though it is now generally accepted that ip3 participates in signal transduction in many important cellular processes, only limited evidence is available for the presence and properties of the ip3r - like protein so far. here, using the immunological methods with an antibody raised against a mammalian inositol 1, 4, 5 - triphophate receptor ( type 1 ), we found that, 1 ) the antibody across - reacted the proteins with about 200kd in microsomes from oryza sativa and about 200kd from arabidopsis thaliana respectively
本實驗用sds - page電泳和免疫印跡的方法，用哺乳動物大鼠三磷酸肌醇受體的多肽做抗體對類三磷酸肌醇受體蛋白鑒定，結果表明：抗體與水稻和擬南芥微粒體蛋白分子量大約為200kd的蛋白交叉反應，同時還發現在水稻微粒體蛋白62kd和擬南芥微粒體蛋白45kd處有交叉反應的蛋白條帶存在，表明在植物中有類三磷酸肌醇受體蛋白的存在；用免疫膠體金方法，發現類三磷酸肌醇受體蛋白主要分佈於液泡膜和細胞質膜上。In the part, the author ' s intention is mensurating the molecular weight of the polypeptides by sodium dodecyl sulfate polyacrylamide gel electro - phoresis. the result shows that one of the polypeptides which we have mensurated is about 17, 000da. the conclusion is that the components which we have separated and purified are small molecule polypeptides. 5
紅褐林蟻多肽蛋白質的分子量測定本部分旨在通過sds -聚丙烯酰胺凝膠電泳測出所得多肽蛋白質的相對分子量，測得其中一組分的相對分子量為17000da左右，結論為所分離、純化的物質是小分子的多肽。Conclusion : phosphoprotein enrichment technique combined with two - dimensional gel electrophoresis is an effective approach to study phosphoproteome, laying a foundation for further investigation of human kidney phosphoproteins
結論：磷酸化蛋白富集技術與二維凝膠電泳技術的結合是研究磷酸化蛋白質組的有效方法，為進一步研究人腎組織磷酸化蛋白奠定基礎。The e. coli strain jm109 was transformed with resultant plasmid pgex - 4t - 1 / 6 - 4. 4. the transformation was induced with iptg, then the total protein from cell extract was analyzed by electrophoresis on a 8 % sds - page in order to validate the gst fusion protein, and the fusion protein is about 90kd
4 .用工ptg誘導含pgex一4t一1 / 6一4的轉化菌，提取初提物中的總蛋白，進行sds一聚丙烯酞胺凝膠電泳，檢測表達的融合蛋白大小越為gokd 。There are at least three kinds of ep in petal : thiol - proteinase, metallo - proteinase, serine proteinase. they gather at the site between 43 kd and 66. 2 kd ( about 52 kd ) on the substrate gel electrophoresis. the optimum ph of thiol - proteinase, metallo - proteinase is ph 6, which are looked as acid ep, the serine proteinase has two forms, one is acid ep ( ph 6 ), and the other is alkali ( ph 10 )
花瓣中都至少有3種內肽酶：巰基蛋白酶、金屬蛋白酶、絲氨酸蛋白酶，三者聚集在底物膠電泳膠條的43kd 66 . 2kd之間的位置，約為52kd ；前兩種最適ph為6 ，是酸性內肽酶，后一種有酸（ ph6 ） 、堿（ ph10 ）兩種存在形式，既是酸性內肽酶又是堿性內肽酶。With highly testing precision and autoraaticity, this system can correct the defects of current image detection methods
該系統測試精度和自動化程度高，較好地解決了電泳膠片圖像檢測手段的不足之處。分享友人