霉蛋白 的英文怎麼說
中文拼音 [méidànbái]
霉蛋白
英文
mucorin-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。Contribution of mucor proteases to sufu maturation
毛霉蛋白酶在腐乳成熟中的作用Studies on property of protease by mucor spp
毛霉蛋白酶特性研究Study on the preparation of mucor protease by solid state fermentation
固體發酵制備毛霉蛋白酶的研究To see what was going on, he labelled the doxycycline - resistant strain with a yellow protein and the sensitive strain with a blue protein
為了觀察進行情況,他用黃色蛋白質標記耐脫氧土霉素的大腸埃希菌,用藍色的蛋白標記對脫氧土霉素敏感的細菌。The experimental results indicated that geotrichum candidum was an ideal microbe species which could produce 18. 6 % protein content after fermentation
試驗結果表明,白地霉對丟糟的轉化較為理想,發酵后可使蛋白質含量高達18 . 6 % 。Geotrichum candidum, candida mycoderma and rhizopus were used as seed speices respectively and daqu distiller ' s grains used as main raw materials ( addition of adequate auxiliary materials as culture medium ) to produce single cell protein by solid fermentation
摘要以白地霉、假絲酵母和根霉為菌種,以大麴丟糟為主要原料,通過添加適當輔料為培養基,經固態發酵方法生產單細胞蛋白。Katie had more bad luck in the autumn of 1996 [ y16 ] when she was placed under the imperius curse by madam rosmerta to carry a cursed opal necklace to someone at hogwarts
凱蒂在1996年秋天為了幫助中了奪魂咒的羅斯默塔夫人,在攜帶被施咒的蛋白石項鏈給霍格沃茲中某人的時候霉運當頭。Doxycycline gums up the assembly line on which proteins are made, whereas ciprofloxacin stops the dna message about how to make each protein being read in the first place, so there is plenty of scope for interference between the two
脫氧土霉素是通過黏附在蛋白質生產線來阻止細菌蛋白質合成的,而環丙沙星是通過阻抑指導蛋白合成的dna遺傳信息的讀取過程來祈禱抗菌作用的。Surface plasmon resonance immunosensor is a relatively new immunoassay technique and has been receiving more and more attention in recent years. however, a major disadvantage of spr for bioanalytical applications is that low concentration or low molecular mass analytes could not be detected directly. therefore, it is a challenging task to develop strategies for improving the detection limit sensitivity of spr. in this paper, authors present a novel strategy for improving the sensitivity of spr immunosensing using streptavidinbiotinylated antibody complex. it is proven that the amplification strategy causes a dramatic improvement of the detection sensitivity. this amplification strategy is based on the construction of a molecular complex between streptavidin and biotin labeled protein. the complex can be formed in a crosslinking network of molecules so that the amplification of response signal will be realized due to the big molecular size of complex
將鏈霉親和素-生物素系統用於表面等離子體共振免疫傳感的信號放大,實時檢測了人免疫球蛋白g higg的蛋白濃度。發生免疫反應的傳感片和生物素化抗體反應后,傳感片表面的一層生物素分子隨后與鏈霉親和素-生物素化抗體復合物中的鏈霉親和素的活性位點發生親和反應,從而使傳感片表面特異健合的物質質量顯著增加,大大提高了免疫檢測的靈敏度和檢測限。免疫反應經放大后,可檢測0 . 00510g ml濃度區間內的higg 。Exposure of cells to the fungal inhibitor wortmannin eliminated focus formation by all repair factors examined, suggesting a role for the phosphoinositide ( pi ) - 3 family of protein kinases in mediating this response
抗真菌藥物渥曼青霉素作用於細胞,能通過檢測到的修復因子消除形成的病灶,這說明了蛋白激酶中磷酸肌醇家族在這一反映中所起的作用A polypeptide with sequence of qkvdssggggs was designed to be a linker between c terminal of penicillin g acylase and n terminal of the coat protein. the ribosome binding site ( rbs sequence ) of psurfscript is also replaced by rbs sequence originating from bacillus subtilis. it was demonstrated that constructed phagemid can still express penicillin g acylase
將包含信號肽和琥珀終止密碼子uag ( amber )的完整巨大芽孢桿菌青霉素g酰化酶基因克隆到噬菌粒psurfscript ,通過引入的11肽連接青霉素g酰化酶的c末端與噬菌體外殼蛋白gp3的n末端。分享友人